WO2000044389A3 - Procede de preparation de vesicules membranaires - Google Patents

Procede de preparation de vesicules membranaires Download PDF

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Publication number
WO2000044389A3
WO2000044389A3 PCT/FR2000/000105 FR0000105W WO0044389A3 WO 2000044389 A3 WO2000044389 A3 WO 2000044389A3 FR 0000105 W FR0000105 W FR 0000105W WO 0044389 A3 WO0044389 A3 WO 0044389A3
Authority
WO
WIPO (PCT)
Prior art keywords
membrane vesicles
vesicles
preparing membrane
preparing
concerns
Prior art date
Application number
PCT/FR2000/000105
Other languages
English (en)
Other versions
WO2000044389A2 (fr
Inventor
Olivier Dhellin
Sebastian Amigorena
Philippe Rameau
Joel Crouzet
Original Assignee
Ap Cells Inc
Inst Nat Sante Rech Med
Inst Curie
Olivier Dhellin
Sebastian Amigorena
Philippe Rameau
Joel Crouzet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ap Cells Inc, Inst Nat Sante Rech Med, Inst Curie, Olivier Dhellin, Sebastian Amigorena, Philippe Rameau, Joel Crouzet filed Critical Ap Cells Inc
Priority to CA002360752A priority Critical patent/CA2360752A1/fr
Priority to JP2000595691A priority patent/JP2002535665A/ja
Priority to US09/890,319 priority patent/US6899863B1/en
Priority to IL14369200A priority patent/IL143692A0/xx
Priority to EA200100819A priority patent/EA003235B1/ru
Priority to AU30561/00A priority patent/AU768322B2/en
Priority to EP00900609A priority patent/EP1143982A2/fr
Publication of WO2000044389A2 publication Critical patent/WO2000044389A2/fr
Publication of WO2000044389A3 publication Critical patent/WO2000044389A3/fr
Priority to HK02102905.8A priority patent/HK1042647A1/zh

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0005—Vertebrate antigens
    • A61K39/0011—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5154—Antigen presenting cells [APCs], e.g. dendritic cells or macrophages

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)

Abstract

La présente invention concerne un procédé de préparation de vésicules membranaires à partir d'un échantillon biologique caractérisé en ce qu'il comprend au moins une étape de traitement de l'échantillon par chromatographie d'échange d'anions et/ou de perméation de gel. L'invention est utilisée pour la préparation de vésicules d'origines et de natures variées, notamment à partir de cellules présentatrices d'antigènes ou de cellules tumorales. L'invention concerne aussi les vésicules ainsi obtenues et leurs utilisations.
PCT/FR2000/000105 1999-01-27 2000-01-19 Procede de preparation de vesicules membranaires WO2000044389A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CA002360752A CA2360752A1 (fr) 1999-01-27 2000-01-19 Procede de preparation de vesicules membranaires
JP2000595691A JP2002535665A (ja) 1999-01-27 2000-01-19 膜小胞を調製するための方法
US09/890,319 US6899863B1 (en) 1999-01-27 2000-01-19 Method for preparing membrane vesicles
IL14369200A IL143692A0 (en) 1999-01-27 2000-01-19 Methods for preparing membrane vesicles
EA200100819A EA003235B1 (ru) 1999-01-27 2000-01-19 Способ получения мембранных пузырьков
AU30561/00A AU768322B2 (en) 1999-01-27 2000-01-19 Method for preparing membrane vesicles
EP00900609A EP1143982A2 (fr) 1999-01-27 2000-01-19 Procede de preparation de vesicules membranaires
HK02102905.8A HK1042647A1 (zh) 1999-01-27 2002-04-17 製備膜囊的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR99/00886 1999-01-27
FR9900886A FR2788780B1 (fr) 1999-01-27 1999-01-27 Procede de preparation de vesicules membranaires

Publications (2)

Publication Number Publication Date
WO2000044389A2 WO2000044389A2 (fr) 2000-08-03
WO2000044389A3 true WO2000044389A3 (fr) 2000-11-16

Family

ID=9541269

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2000/000105 WO2000044389A2 (fr) 1999-01-27 2000-01-19 Procede de preparation de vesicules membranaires

Country Status (11)

Country Link
US (1) US6899863B1 (fr)
EP (1) EP1143982A2 (fr)
JP (1) JP2002535665A (fr)
CN (1) CN1355704A (fr)
AU (1) AU768322B2 (fr)
CA (1) CA2360752A1 (fr)
EA (1) EA003235B1 (fr)
FR (1) FR2788780B1 (fr)
HK (1) HK1042647A1 (fr)
IL (1) IL143692A0 (fr)
WO (1) WO2000044389A2 (fr)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7704964B2 (en) 2001-08-17 2010-04-27 Exothera L.L.C. Methods and compounds for the targeting of protein to exosomes
EP1766053A4 (fr) * 2004-06-02 2007-12-12 Sourcepharm Inc Microvesicules contenant de l'arn et methodes associees
US8021847B2 (en) 2004-06-02 2011-09-20 Proxy Life Science Holdings, Inc. Microvesicle-based compositions and methods
WO2007127848A1 (fr) * 2006-04-26 2007-11-08 University Of Louisville Research Foundation, Inc Isolation de vésicules membranaires de fluides biologiques et procédés d'utilisation
US8455188B2 (en) * 2007-01-26 2013-06-04 University Of Louisville Research Foundation, Inc. Modification of exosomal components for use as a vaccine
EP2176665B1 (fr) 2007-08-16 2016-03-02 The Royal Institution for the Advancement of Learning / McGill University Microvesicules issues d'une cellule tumorale
US20100255514A1 (en) * 2007-08-16 2010-10-07 The Royal Institution For The Advancement Of Learning/Mcgill University Tumor cell-derived microvesicles
WO2011009104A1 (fr) 2009-07-16 2011-01-20 The General Hospital Corporation Analyse d'acides nucléiques
DK2245199T3 (da) * 2008-02-01 2014-02-03 Gen Hospital Corp Anvendelse af mikrovesikler i diagnosticering, prognosticering og behandling af medicinske sygdomme og tilstande
US20220162704A1 (en) * 2008-02-01 2022-05-26 The General Hospital Corporation Methods of preparing microvesicle micrornas from bodily fluids
US11266736B2 (en) 2008-04-17 2022-03-08 Vin De Bona Trading Company Pte Ltd Method of painting micro vesicles
EP2358912B1 (fr) 2008-10-30 2016-10-12 Caris Life Sciences Switzerland Holdings GmbH Procédés d'évaluation de motifs arn
EP2730662A1 (fr) 2008-11-12 2014-05-14 Caris Life Sciences Luxembourg Holdings Procédés et systèmes permettant d'utiliser des exosomes pour déterminer des phénotypes
WO2011031892A1 (fr) 2009-09-09 2011-03-17 The General Hospital Corporation Utilisation de microvésicules dans l'analyse de mutations kras
WO2011031877A1 (fr) 2009-09-09 2011-03-17 The General Hospital Corporation Utilisation de microvésicules dans l'analyse de profils d'acide nucléique
EP2496711B1 (fr) 2009-11-02 2016-02-17 Agency For Science, Technology And Research Procedes de surveillance des etats cellulaires et de immortaliser des cellules souches mésenchymateuses
AU2010324594B2 (en) 2009-11-30 2016-09-15 Caris Life Sciences Switzerland Holdings Gmbh Methods and systems for isolating, storing, and analyzing vesicles
US20120315324A1 (en) * 2010-02-05 2012-12-13 University Of Louisville Research Foundation, Inc. Exosomal compositions and methods for the treatment of disease
CN103237901B (zh) 2010-03-01 2016-08-03 卡里斯生命科学瑞士控股有限责任公司 用于治疗诊断的生物标志物
AU2011237669B2 (en) 2010-04-06 2016-09-08 Caris Life Sciences Switzerland Holdings Gmbh Circulating biomarkers for disease
EP2591359B1 (fr) 2010-07-07 2017-03-01 Aethlon Medical Inc Procédés permettant de quantifier des exosomes
WO2012031008A2 (fr) 2010-08-31 2012-03-08 The General Hospital Corporation Matières biologiques liées au cancer dans des microvésicules
AU2011326366B2 (en) 2010-11-10 2017-02-23 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
SG190450A1 (en) * 2010-12-20 2013-06-28 Agency Science Tech & Res Method of purifying exosomes
WO2012155014A1 (fr) 2011-05-11 2012-11-15 Exosome Diagnostics, Inc. Extraction d'acide nucléiques à partir de matériaux biologiques hétérogènes
EP3564393A1 (fr) 2011-06-21 2019-11-06 Alnylam Pharmaceuticals, Inc. Analyses et procédés pour déterminer l'activité d'un agent thérapeutique chez un sujet
GB201110777D0 (en) * 2011-06-24 2011-08-10 Aqua Bio Technology Asa Methods and uses
GB201110783D0 (en) 2011-06-24 2011-08-10 Aqua Bio Technology Asa Methods and uses
ES2395793B1 (es) * 2011-07-28 2013-12-27 Consejo Superior De Investigaciones Científicas (Csic) Procedimiento de obtención y usos de vesículas de membrana de un organismo marino para su utilización industrial.
EP2748335B1 (fr) 2011-08-22 2018-10-03 Exosome Diagnostics, Inc. Marqueurs biologiques d'urine
EP2776587A4 (fr) 2011-11-10 2015-07-15 Exosome Diagnostics Inc Dosage du liquide céphalorachidien
WO2013079701A2 (fr) 2011-11-30 2013-06-06 University Of Bremen Expression d'arnmi dans le tissu placentaire
CN103479682B (zh) * 2012-06-14 2015-03-11 苏州恒宇生物科技有限公司 一种植物来源活性成分-纳米级膜性囊泡的制备方法
EP3299814A3 (fr) * 2012-07-19 2018-04-11 Atlantic Cancer Research Institute Procédé d'isolement de microvésicules
JP2015529450A (ja) * 2012-07-19 2015-10-08 リニューロン・リミテッドＲｅＮｅｕｒｏｎ Ｌｉｍｉｔｅｄ 幹細胞微粒子
US10231997B2 (en) 2012-08-15 2019-03-19 The University Of Chicago Exosome-based therapeutics against neurodegenerative disorders
CA2883220C (fr) 2012-08-30 2022-09-20 Exosome Diagnostics, Inc. Temoins pour dosages d'acide nucleique
EP2903597B1 (fr) 2012-10-03 2019-12-18 Exosome Diagnostics Inc. Utilisation de microvésicules dans le diagnostic, le pronostic et le traitement de maladies et d'états médicaux
DK2906717T3 (en) 2012-10-11 2019-04-01 Vin De Bona Trading Company Pte Ltd PROCEDURE FOR PAINTING MICROVESICS
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
US20150353920A1 (en) 2013-01-03 2015-12-10 Exosome Diagnostics, Inc. Methods for isolating microvesicles
US11513125B2 (en) 2013-03-14 2022-11-29 Pioma, Inc. Microvesicle histone H2AX as a biomarker for genotoxic stress
US11274139B2 (en) * 2013-04-12 2022-03-15 Evox Therapeutics Ltd Therapeutic delivery vesicles
AU2014305994B2 (en) 2013-08-06 2019-02-21 Exosome Diagnostics, Inc. Urine biomarker cohorts, gene expression signatures, and methods of use thereof
GB201317887D0 (en) 2013-10-09 2013-11-20 Reneuron Ltd Product
CN105980615A (zh) 2013-08-28 2016-09-28 卡里斯生命科学瑞士控股有限公司 寡核苷酸探针及其用途
EP3052616A4 (fr) * 2013-10-02 2017-03-29 Paradigm Biopharmaceuticals Limited Procédé de production d'exosomes
WO2015060446A1 (fr) 2013-10-25 2015-04-30 凸版印刷株式会社 Dispositif de récupération de vésicule à membrane, procédé de récupération de vésicule à membrane et procédé d'analyse de vésicule à membrane
FR3014198B1 (fr) * 2013-12-03 2017-03-03 Biomerieux Sa Procede d'isolement d'exosomes
WO2015088286A1 (fr) * 2013-12-12 2015-06-18 사회복지법인 삼성생명공익재단 Composition pharmaceutique pour traiter des maladies cérébrovasculaires, contenant un exosome dérivé de cellules souches en tant que substance active
US9919011B2 (en) 2014-03-18 2018-03-20 Samsung Life Public Welfare Foundation Method for treating an inflammatory brain disease comprising administering a stem cell-derived exosome
US10844436B2 (en) 2014-04-01 2020-11-24 Cornell University Use of double-stranded DNA in exosomes: a novel biomarker in cancer detection
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
AU2015287763B2 (en) 2014-07-09 2019-08-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
EP3808756A1 (fr) 2014-07-17 2021-04-21 The Trustees of The University of Pennsylvania Procédés permettant d'utiliser des exosomes pour surveiller l'état d'un organe transplanté
JP6597614B2 (ja) 2014-07-24 2019-10-30 凸版印刷株式会社 脂質膜構造体、脂質膜構造体固定化担体、及び胞体の融合方法
EP3112474A1 (fr) 2015-06-29 2017-01-04 Evonik Degussa GmbH Procédé de détection d'entérite nécrotique aviaire
WO2016145128A1 (fr) 2015-03-09 2016-09-15 Caris Science, Inc. Sondes oligonucléotidiques et utilisations de celles-ci
EP4218780A1 (fr) * 2015-06-12 2023-08-02 Hudson Institute of Medical Research Exosomes allogènes amniotiques pour le traitement de maladies
US10590425B2 (en) 2015-06-29 2020-03-17 Caris Science, Inc. Therapeutic oligonucleotides
AU2016298317B2 (en) 2015-07-28 2021-02-18 Caris Science, Inc. Targeted oligonucleotides
EP3165926A1 (fr) 2015-11-06 2017-05-10 Humanitas Mirasole S.p.A. Procédé de caractérisation des microvésicules spécifiques de cellule
CN106811428A (zh) * 2015-11-30 2017-06-09 复旦大学 一种提取细菌外排膜囊泡的方法
RU2608509C1 (ru) * 2016-02-11 2017-01-18 Елена Сергеевна Кастарнова Способ получения экзосом из крови
US20190091307A1 (en) 2016-03-03 2019-03-28 Institut Gustave Roussy Ptps-based vaccines against cancer
US11702702B2 (en) 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
EP3443117A1 (fr) 2016-04-15 2019-02-20 Exosome Diagnostics, Inc. Détection par plasma d'acides nucléiques de kinase de lymphome anaplasique (alk) et de transcrits de fusion d'alk et leurs utilisations dans le diagnostic et le traitement du cancer
WO2018057820A1 (fr) 2016-09-21 2018-03-29 Predicine, Inc. Systèmes et procédés de détection combinée d'altérations génétiques
EP3452613B1 (fr) 2016-05-05 2021-12-29 Exosome Diagnostics, Inc. Profilage d'acides nucléiques de microvésicules et leurs utilisations en tant que signatures en diagnostic de rejet de greffe de rein
WO2017197399A1 (fr) 2016-05-13 2017-11-16 Exosome Diagnostics, Inc. Procédés automatisés et manuels pour l'isolement de vésicules extracellulaires et l'isolement conjoint d'adn acellulaire à partir de bioliquides
EP3467059B1 (fr) 2016-05-24 2022-02-23 Japanese Foundation For Cancer Research Procédé de récupération de vésicules extracellulaires et récipient destiné à des vésicules extracellulaires
US11299733B2 (en) 2016-07-15 2022-04-12 University Of Massachusetts Process of delivering small RNAs to sperm
IL247368A0 (en) 2016-08-18 2016-11-30 Yeda Res & Dev Diagnostic and therapeutic uses of exosomes
CN110191962A (zh) 2016-10-21 2019-08-30 外来体诊断公司 外来体相关核酸的测序和分析
AU2017366813B2 (en) 2016-11-30 2023-04-20 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal RNA and cell free DNA from non-small cell lung cancer patients
RU2748234C2 (ru) * 2016-12-23 2021-05-21 Эксофарм Лтд Способы и композиции для очистки или выделения микровезикул и экзосом
EP3562959A2 (fr) 2017-01-02 2019-11-06 Exosome Diagnostics, Inc. Procédés de distinction d'arn et d'adn dans une préparation combinée
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
KR20200006108A (ko) 2017-05-12 2020-01-17 에보니크 오퍼레이션즈 게엠베하 동물에서 씨. 퍼프린젠스 유발된 질환을 검출하는 방법
JP7225121B2 (ja) 2017-05-17 2023-02-20 エクソサム ダイアグノスティクス，インコーポレイティド 微小胞核酸および／またはタンパク質、並びに腎移植拒絶反応マーカーとしてのその使用
EP3652315A4 (fr) 2017-07-12 2021-09-01 Exosome Diagnostics, Inc. Procédés d'isolement et d'enrichissement de populations de vésicules extracellulaires dérivées de biofluides, et procédés d'utilisation associés
US11345957B2 (en) 2017-07-18 2022-05-31 Exosome Diagnostics, Inc. Methods of treating glioblastoma in a subject informed by exosomal RNA signatures
US11904259B2 (en) * 2017-07-26 2024-02-20 Rosetta Exosome Method for isolating extracellular vesicles using cations
TR201716062A2 (tr) * 2017-10-18 2019-05-21 Tuerkiye Bilimsel Ve Teknolojik Arastirma Kurumu Tuebitak Bi̇r eksozomal terapöti̇k taşiyici üreti̇m yöntemi̇ ve bu yönteme uygun bi̇r şeki̇lde elde edi̇len bi̇r eksozomal terapöti̇k taşiyici
GB201717446D0 (en) 2017-10-24 2017-12-06 Evox Therapeutics Ltd Affinity purification of engineering extracellular vesicles
US20200339987A1 (en) 2017-11-09 2020-10-29 Alnylam Pharmaceuticals, Inc. Assays and methods for determining expression of the lect2 gene
WO2019104169A1 (fr) * 2017-11-21 2019-05-31 Purina Animal Nutrition Llc Procédés de purification d'exosomes
WO2019160519A2 (fr) 2018-02-15 2019-08-22 Yeditepe Universitesi Procédé d'isolement d'exosomes par un système de fluide à deux phases
US20200378932A1 (en) * 2018-02-20 2020-12-03 Korea University Research And Business Foundation Multi-column for isolating exosomes and exosome isolation method
CA3092519A1 (fr) 2018-03-02 2019-09-06 Evonik Operations Gmbh Procede in vitro de detection d'une defaillance de la barriere intestinale chez des animaux par determination de l'ovotransferrine
US20210230701A1 (en) 2018-06-06 2021-07-29 Exosome Diagnostics, Inc. Methods for developing urine biomarkers and for detecting bladder cancer
GB201809622D0 (en) 2018-06-12 2018-07-25 Evox Therapeutics Ltd Engineering extracellular vesicles for affinity purification
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
WO2020027185A1 (fr) * 2018-07-31 2020-02-06 国立大学法人三重大学 Procédé de production d'exosomes
EP3884045B1 (fr) 2018-11-20 2023-01-11 Exosome Diagnostics, Inc. Procédés pour le contrôle interne d'isolation de microvésicules
IT201800021226A1 (it) 2018-12-27 2020-06-27 Supsi Scuola Univ Professionale Della Svizzera Italiana Processo e dispositivo per produrre esosomi
CN109856259A (zh) * 2018-12-29 2019-06-07 南通大学附属医院 一种血清胰腺癌外泌体特异蛋白及其再验证过程
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JP2023104650A (ja) * 2022-01-18 2023-07-28 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
WO2024024336A1 (fr) * 2022-07-28 2024-02-01 株式会社ダイセル Dispositif de purification et de concentration d'un liquide comprenant une substance utile infime et procédé de production d'un liquide purifié et concentré d'une substance utile infime l'utilisant
CN117070450B (zh) * 2023-10-12 2024-01-16 哈药集团生物工程有限公司 一种外泌体的纯化方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005900A1 (fr) * 1995-08-03 1997-02-20 Rijksuniversiteit Te Leiden Vesicules presentant des antigenes obtenues a partir de cellules
FR2766205A1 (fr) * 1997-07-16 1999-01-22 Inst Nat Sante Rech Med Nouveau procede de sensibilisation de cellules presentatrices d'antigene et nouveaux moyens pour la mise en oeuvre du procede

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2877160B2 (ja) * 1987-07-29 1999-03-31 ザ リポソーム カンパニー，インコーポレイテッド 粒子のサイズによる分離方法
US6165785A (en) * 1996-05-24 2000-12-26 University Of Cincinnati Bone marrow cultures for developing suppressor and stimulator cells for research and therapeutic applications
AU2710897A (en) * 1996-05-31 1998-01-05 Whitaker Corporation, The Rechargeable battery connector

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997005900A1 (fr) * 1995-08-03 1997-02-20 Rijksuniversiteit Te Leiden Vesicules presentant des antigenes obtenues a partir de cellules
FR2766205A1 (fr) * 1997-07-16 1999-01-22 Inst Nat Sante Rech Med Nouveau procede de sensibilisation de cellules presentatrices d'antigene et nouveaux moyens pour la mise en oeuvre du procede
WO1999003499A1 (fr) * 1997-07-16 1999-01-28 Institut National De La Sante Et De La Recherche Medicale Vesicule cellulaire denommee ''exosome'', leur preparation et utilisation dans la stimulation d'une reponse immunitaire

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
G. RAPOSO ET AL.: "B LYMPHOCYTES SECRETE ANTIGEN-PRESENTING VESICLES.", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 183, March 1996 (1996-03-01), NEW YORK, N.Y., US, pages 1161 - 1172, XP002060486 *

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EP1143982A2 (fr) 2001-10-17
IL143692A0 (en) 2002-04-21
EA200100819A1 (ru) 2001-12-24
CN1355704A (zh) 2002-06-26
FR2788780B1 (fr) 2001-03-30
CA2360752A1 (fr) 2000-08-03
JP2002535665A (ja) 2002-10-22
WO2000044389A2 (fr) 2000-08-03
HK1042647A1 (zh) 2002-08-23
US6899863B1 (en) 2005-05-31
EA003235B1 (ru) 2003-02-27

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