EA010414B1 - Сочетание реагентов и способ непосредственного измерения при комнатной температуре содержания холестерина липопротеинов низкой плотности с использованием индикаторной полоски - Google Patents
Сочетание реагентов и способ непосредственного измерения при комнатной температуре содержания холестерина липопротеинов низкой плотности с использованием индикаторной полоски Download PDFInfo
- Publication number
- EA010414B1 EA010414B1 EA200601415A EA200601415A EA010414B1 EA 010414 B1 EA010414 B1 EA 010414B1 EA 200601415 A EA200601415 A EA 200601415A EA 200601415 A EA200601415 A EA 200601415A EA 010414 B1 EA010414 B1 EA 010414B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- layer
- cholesterol
- indicator strip
- indicator
- solution
- Prior art date
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims abstract description 114
- 235000012000 cholesterol Nutrition 0.000 title claims abstract description 56
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 32
- 108010007622 LDL Lipoproteins Proteins 0.000 title claims abstract description 18
- 102000007330 LDL Lipoproteins Human genes 0.000 title claims abstract description 18
- 238000005259 measurement Methods 0.000 title claims description 7
- 238000000034 method Methods 0.000 title description 29
- 108010028554 LDL Cholesterol Proteins 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims description 47
- SPIUHJINJWTDQE-UHFFFAOYSA-N cyclohexaamylose polysulfate Chemical compound O1C(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC(C(OS(O)(=O)=O)C2OS(O)(=O)=O)C(COS(=O)(=O)O)OC2OC(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC2C(OS(O)(=O)=O)C(OS(O)(=O)=O)C1OC2COS(O)(=O)=O SPIUHJINJWTDQE-UHFFFAOYSA-N 0.000 claims description 38
- 229960000633 dextran sulfate Drugs 0.000 claims description 38
- 239000004094 surface-active agent Substances 0.000 claims description 24
- 210000004369 blood Anatomy 0.000 claims description 22
- 239000008280 blood Substances 0.000 claims description 22
- 102000004895 Lipoproteins Human genes 0.000 claims description 15
- 108090001030 Lipoproteins Proteins 0.000 claims description 15
- 210000003743 erythrocyte Anatomy 0.000 claims description 11
- 229910052751 metal Inorganic materials 0.000 claims description 9
- 239000002184 metal Substances 0.000 claims description 9
- 210000002966 serum Anatomy 0.000 claims description 8
- 229920001577 copolymer Polymers 0.000 claims description 7
- 210000002381 plasma Anatomy 0.000 claims description 7
- 229920001400 block copolymer Polymers 0.000 claims description 6
- 230000000903 blocking effect Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229920000447 polyanionic polymer Polymers 0.000 claims description 6
- -1 polyoxypropylene Polymers 0.000 claims description 6
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical class O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 6
- 150000001768 cations Chemical class 0.000 claims description 5
- 229920001451 polypropylene glycol Polymers 0.000 claims description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 4
- 150000001412 amines Chemical class 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- 239000011777 magnesium Substances 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 108010022197 lipoprotein cholesterol Proteins 0.000 claims description 3
- GJMPSRSMBJLKKB-UHFFFAOYSA-N 3-methylphenylacetic acid Chemical group CC1=CC=CC(CC(O)=O)=C1 GJMPSRSMBJLKKB-UHFFFAOYSA-N 0.000 claims description 2
- 239000013543 active substance Substances 0.000 claims description 2
- 229940117957 triethanolamine hydrochloride Drugs 0.000 claims description 2
- 229920001353 Dextrin Polymers 0.000 claims 1
- 239000004375 Dextrin Substances 0.000 claims 1
- 235000019425 dextrin Nutrition 0.000 claims 1
- 238000002845 discoloration Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 abstract description 4
- 102100030797 Conserved oligomeric Golgi complex subunit 2 Human genes 0.000 abstract 1
- 101000920113 Homo sapiens Conserved oligomeric Golgi complex subunit 2 Proteins 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 91
- 239000000872 buffer Substances 0.000 description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 41
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 40
- 239000000600 sorbitol Substances 0.000 description 40
- 238000004458 analytical method Methods 0.000 description 38
- 239000008367 deionised water Substances 0.000 description 36
- 229910021641 deionized water Inorganic materials 0.000 description 36
- 239000000523 sample Substances 0.000 description 36
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 33
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 32
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 31
- 229930006000 Sucrose Natural products 0.000 description 31
- 239000005720 sucrose Substances 0.000 description 31
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 20
- 235000019341 magnesium sulphate Nutrition 0.000 description 20
- 239000000126 substance Substances 0.000 description 17
- 229910001629 magnesium chloride Inorganic materials 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 15
- 239000000463 material Substances 0.000 description 13
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 12
- 238000001035 drying Methods 0.000 description 7
- 229920000858 Cyclodextrin Polymers 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- 230000002708 enhancing effect Effects 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 6
- 230000007480 spreading Effects 0.000 description 6
- 238000003892 spreading Methods 0.000 description 6
- 239000002202 Polyethylene glycol Substances 0.000 description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 description 5
- 125000002091 cationic group Chemical group 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 208000029078 coronary artery disease Diseases 0.000 description 5
- 238000005470 impregnation Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 description 5
- 108010004103 Chylomicrons Proteins 0.000 description 4
- 238000001502 gel electrophoresis Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 229940051841 polyoxyethylene ether Drugs 0.000 description 4
- 229920000056 polyoxyethylene ether Polymers 0.000 description 4
- 108010089254 Cholesterol oxidase Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 239000011152 fibreglass Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 3
- 150000003626 triacylglycerols Chemical class 0.000 description 3
- 229920000428 triblock copolymer Polymers 0.000 description 3
- 238000005199 ultracentrifugation Methods 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 108010010234 HDL Lipoproteins Proteins 0.000 description 2
- 102000015779 HDL Lipoproteins Human genes 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 2
- 108010055297 Sterol Esterase Proteins 0.000 description 2
- 102000000019 Sterol Esterase Human genes 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 238000012993 chemical processing Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 2
- 229910052748 manganese Inorganic materials 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- 239000000693 micelle Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 229920002492 poly(sulfone) Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- ODLHGICHYURWBS-LKONHMLTSA-N trappsol cyclo Chemical compound CC(O)COC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)COCC(O)C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1COCC(C)O ODLHGICHYURWBS-LKONHMLTSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- ALSTYHKOOCGGFT-KTKRTIGZSA-N (9Z)-octadecen-1-ol Chemical compound CCCCCCCC\C=C/CCCCCCCCO ALSTYHKOOCGGFT-KTKRTIGZSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000001116 FEMA 4028 Substances 0.000 description 1
- 108010023302 HDL Cholesterol Proteins 0.000 description 1
- 101000581326 Homo sapiens Mediator of DNA damage checkpoint protein 1 Proteins 0.000 description 1
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
- 240000002329 Inga feuillei Species 0.000 description 1
- 102100027643 Mediator of DNA damage checkpoint protein 1 Human genes 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 108010069201 VLDL Cholesterol Proteins 0.000 description 1
- 108010062497 VLDL Lipoproteins Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 229920006318 anionic polymer Polymers 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000011175 beta-cyclodextrine Nutrition 0.000 description 1
- 229960004853 betadex Drugs 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 229960002086 dextran Drugs 0.000 description 1
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 1
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 125000000040 m-tolyl group Chemical group [H]C1=C([H])C(*)=C([H])C(=C1[H])C([H])([H])[H] 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000000820 nonprescription drug Substances 0.000 description 1
- 229940055577 oleyl alcohol Drugs 0.000 description 1
- XMLQWXUVTXCDDL-UHFFFAOYSA-N oleyl alcohol Natural products CCCCCCC=CCCCCCCCCCCO XMLQWXUVTXCDDL-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000005297 pyrex Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- DSDAICPXUXPBCC-MWDJDSKUSA-N trimethyl-β-cyclodextrin Chemical compound COC[C@H]([C@H]([C@@H]([C@H]1OC)OC)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O3)[C@H](OC)[C@H]2OC)COC)O[C@@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@@H]3O[C@@H]1COC DSDAICPXUXPBCC-MWDJDSKUSA-N 0.000 description 1
- 229960004418 trolamine Drugs 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
- G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54168104P | 2004-02-03 | 2004-02-03 | |
| US10/962,272 US7435577B2 (en) | 2004-02-03 | 2004-10-11 | Direct measurement of chlolesterol from low density lipoprotein with test strip |
| PCT/US2005/003234 WO2005074609A2 (en) | 2004-02-03 | 2005-02-03 | Test strip composition and method to measure cholesterol from low density lipoproteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200601415A1 EA200601415A1 (ru) | 2006-12-29 |
| EA010414B1 true EA010414B1 (ru) | 2008-08-29 |
Family
ID=34811458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200601415A EA010414B1 (ru) | 2004-02-03 | 2005-02-03 | Сочетание реагентов и способ непосредственного измерения при комнатной температуре содержания холестерина липопротеинов низкой плотности с использованием индикаторной полоски |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7435577B2 (enExample) |
| EP (1) | EP1725650B1 (enExample) |
| JP (1) | JP4785753B2 (enExample) |
| CN (1) | CN104931712A (enExample) |
| AU (1) | AU2005209856B2 (enExample) |
| BR (1) | BRPI0507386B1 (enExample) |
| CA (1) | CA2554776C (enExample) |
| EA (1) | EA010414B1 (enExample) |
| IL (1) | IL177226A (enExample) |
| PL (1) | PL1725650T3 (enExample) |
| WO (1) | WO2005074609A2 (enExample) |
| ZA (1) | ZA200606561B (enExample) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060062688A1 (en) * | 2004-02-03 | 2006-03-23 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| US7625721B2 (en) * | 2004-02-03 | 2009-12-01 | Polymer Technology Systems, Inc. | Non-precipitating bodily fluid analysis system |
| US8465696B2 (en) * | 2004-02-03 | 2013-06-18 | Polymer Technology Systems, Inc. | Dry test strip with controlled flow and method of manufacturing same |
| US7435577B2 (en) | 2004-02-03 | 2008-10-14 | Polymer Technology Systems, Inc. | Direct measurement of chlolesterol from low density lipoprotein with test strip |
| US7491542B2 (en) * | 2004-07-12 | 2009-02-17 | Kim Scheuringer | Test device for determining the concentration of LDL-cholesterol in a sample |
| US20060062690A1 (en) * | 2004-08-17 | 2006-03-23 | Polymer Technology Systems, Inc. | Apparatus and method of manufacturing bodily fluid test strip |
| WO2006033733A1 (en) * | 2004-08-17 | 2006-03-30 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| JP5247158B2 (ja) * | 2008-01-16 | 2013-07-24 | パナソニック株式会社 | 試料溶液分析方法および試料溶液分析装置 |
| US20110200498A1 (en) * | 2008-06-17 | 2011-08-18 | Polymer Technology Systems, Inc. | System and method for packaging dry test strips |
| US8685663B2 (en) * | 2008-11-14 | 2014-04-01 | Kyowa Medex Co., Ltd. | Method, reagent and kit for measuring cholesterol in low-density lipoproteins |
| KR101058743B1 (ko) * | 2009-06-04 | 2011-08-24 | 주식회사 인포피아 | 콜레스테롤 측정 스트립 및 이를 이용한 콜레스테롤 검출 방법 |
| KR101110561B1 (ko) * | 2009-06-04 | 2012-02-15 | 주식회사 인포피아 | 바이오 센서 |
| KR101203385B1 (ko) * | 2009-06-04 | 2012-11-21 | 주식회사 인포피아 | 혈액의 퍼짐성이 향상된 측정 스트립 |
| JP5460183B2 (ja) * | 2009-08-28 | 2014-04-02 | パナソニック株式会社 | 分析用試薬および分析用デバイス |
| US20120107850A1 (en) * | 2009-07-15 | 2012-05-03 | Panasonic Corporation | Analysis reagent and analysis device having the analysis reagent carried therein |
| US8012770B2 (en) | 2009-07-31 | 2011-09-06 | Invisible Sentinel, Inc. | Device for detection of antigens and uses thereof |
| WO2011044574A1 (en) | 2009-10-09 | 2011-04-14 | Invisible Sentinel | Device for detection of antigens and uses thereof |
| JP5285000B2 (ja) * | 2010-02-23 | 2013-09-11 | 富士フイルム株式会社 | 低密度リポ蛋白コレステロールの測定方法 |
| JP5832995B2 (ja) * | 2010-04-30 | 2015-12-16 | 協和メデックス株式会社 | 低密度リポ蛋白中のコレステロールの測定方法、測定用試薬及び測定用キット |
| AU2012211141B2 (en) | 2011-01-27 | 2016-11-03 | Invisible Sentinel, Inc. | Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof |
| JP6018177B2 (ja) * | 2011-04-22 | 2016-11-02 | ポリマー テクノロジー システムズ インコーポレーテッド | 乾燥試験ストリップのための血液分離システムおよび方法 |
| CA2866379C (en) | 2012-03-09 | 2020-10-13 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| DE112013002316T5 (de) * | 2012-05-04 | 2015-04-23 | Polymer Technology Systems, Inc. | Systeme und Verfahren für Assays von im nicht-nüchternen Zustand gewonnenem LDL-Cholesterin |
| EP3114482B1 (en) | 2014-03-07 | 2020-10-07 | The Regents of the University of California | Devices for integrating analyte extraction, concentration and detection |
| PT3164213T (pt) * | 2014-07-03 | 2024-03-25 | Abionic Sa | Cápsula para quantificação molecular rápida de uma amostra de fluido tal como sangue total |
| BR112018001256A2 (pt) * | 2015-07-23 | 2018-09-11 | Gentian Diagnostics As | método de ensaio para avaliação de uma ou mais subclasses de células sanguíneas de interesse (bcoi), método para avaliar a quantidade de receptores de cd4 localizados nas superfícies de células cd4+ e opcionalmente, para avaliar a quantidade de receptores de cd8 localizados nas superfícies de células, dispositivo de ensaio de fluxo vertical para realizar um método, e, uso de um dispositivo. |
| EP3345001A4 (en) | 2015-09-04 | 2019-07-17 | The Regents of the University of California | METHOD AND DEVICES FOR ANALYTICAL COLLECTION, EXTRACTION, CONCENTRATION AND DETECTION FOR CLINICAL APPLICATIONS |
| EP4202439B1 (en) | 2016-06-09 | 2025-02-19 | The Regents of the University of California | Method of purifying and amplifying a nucleic acid |
| WO2018039139A1 (en) | 2016-08-22 | 2018-03-01 | The Regents Of The University Of California | Hydrogel platform for aqueous two-phase concentration of a target to enhance its detection |
| WO2018138139A1 (en) * | 2017-01-24 | 2018-08-02 | Gentian As | Method for assessing cell surface receptors of blood cells |
| US11209427B2 (en) | 2017-03-27 | 2021-12-28 | The Regents Of The University Of California | Semi-quantitative lateral-flow immunoassay for the detection of CSF leaks |
| US11338294B2 (en) | 2017-04-27 | 2022-05-24 | Polybiomics, Inc. | Orthogonal polybiosensing and imaging systems |
| EP3677688B1 (en) | 2017-09-01 | 2024-08-07 | Hitachi Chemical Diagnostics Systems Co., Ltd. | Method for measuring cholesterol in low-density lipoprotein, use of a measurement reagent, and of a measurement kit |
| CN107402209B (zh) * | 2017-09-15 | 2020-04-07 | 光景生物科技(苏州)有限公司 | 一种用于检测血清中低密度脂蛋白胆固醇的试纸条及其制备方法 |
| JP6454950B1 (ja) * | 2018-03-29 | 2019-01-23 | 株式会社明日香特殊検査研究所 | 粥状動脈硬化による心筋梗塞や脳梗塞のリスクが高いと判定する易酸化性ＶＬＤＬ（ＶＬＤＬ ｓｕｓｃｅｐｔｉｂｉｌｉｔｙ ｔｏ ｏｘｉｄａｔｉｏｎ）および易酸化性ＬＤＬ（ＬＤＬ ｓｕｓｃｅｐｔｉｂｉｌｉｔｙ ｔｏ ｏｘｉｄａｔｉｏｎ）の簡便な測定方法および測定装置 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6342364B1 (en) * | 1999-11-22 | 2002-01-29 | Matsushita Electric Industrial Co., Ltd. | Cholesterol sensor and method of determining cholesterol |
| US20030003522A1 (en) * | 2001-06-29 | 2003-01-02 | International Business Machines Corporation | Method, system, and apparatus for measurement and recording of blood chemistry and other physiological measurements |
| US20030092102A1 (en) * | 2000-01-31 | 2003-05-15 | Human Genome Sciences, Inc. | Nucleic acids, proteins, and antibodies |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3809617A (en) | 1972-11-15 | 1974-05-07 | American Cyanamid Co | Device for detecting anticholinesterase materials |
| US4178153A (en) | 1977-11-21 | 1979-12-11 | Damon Corporation | Method and apparatus for chemical spot test analysis |
| DE3029579C2 (de) | 1980-08-05 | 1985-12-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Mittel zur Abtrennung von Plasma oder Serum aus Vollblut |
| DE3217925A1 (de) | 1982-05-13 | 1983-11-17 | B. Braun Melsungen Ag, 3508 Melsungen | Verfahren zur selektiven extrakorporalen praezipitation von low-density lipoproteinen aus vollserum oder plasma fuer diagnostische zwecke |
| DE3401932A1 (de) | 1984-01-20 | 1985-08-01 | Collmann GmbH & Co, Spezialmaschinenbau KG, 2400 Lübeck | Verfahren und vorrichtung zum darbieten von verstaerkungseinlagen fuer schleifscheiben |
| DE3530993A1 (de) | 1985-08-30 | 1987-03-05 | Miles Lab | Teststreifen mit festlegbarer probenaufnahmekapazitaet |
| TW203120B (enExample) | 1985-10-04 | 1993-04-01 | Abbott Lab | |
| DE3782597T3 (de) | 1986-09-18 | 2002-09-05 | Pacific Biotech Inc., San Diego | Immunodiagnostische Vorrichtung. |
| DE3787078T2 (de) | 1986-11-24 | 1993-12-09 | Abbott Lab | Proberichtwirkung für analytisches Festphasengerät. |
| US4774192A (en) | 1987-01-28 | 1988-09-27 | Technimed Corporation | A dry reagent delivery system with membrane having porosity gradient |
| JPH0264455A (ja) | 1988-08-31 | 1990-03-05 | Konica Corp | 体液検査素子及びその製造方法 |
| US5135716A (en) | 1989-07-12 | 1992-08-04 | Kingston Diagnostics, L.P. | Direct measurement of HDL cholesterol via dry chemistry strips |
| DE3929032C2 (de) | 1989-09-01 | 1998-09-03 | Boehringer Mannheim Gmbh | Verfahren zur Bestimmung von HDL-Cholesterin mittels eines Schnelldiagnostikums mit integriertem Fraktionierschritt |
| AU634814B2 (en) | 1989-09-14 | 1993-03-04 | Terumo Kabushiki Kaisha | Method for determination of ion concentration, specific gravity, or osmotic pressure of solution and apparatus therefor |
| JPH04324347A (ja) | 1991-04-24 | 1992-11-13 | Terumo Corp | 試験具 |
| GR1001410B (el) * | 1991-08-02 | 1993-11-30 | Ortho Pharma Corp | Επινόημα & μέ?οδος διεξαγωγής βιολογικών δοκιμασιών περιέχοντας σύστημα μοριακής διαλογής. |
| US5286626A (en) * | 1991-12-13 | 1994-02-15 | Actimed Laboratories, Inc. | Process and apparatus for direct determination of low density lipoprotein |
| US5460974A (en) | 1992-10-13 | 1995-10-24 | Miles Inc. | Method of assaying whole blood for HDL cholesterol |
| US5401466A (en) * | 1993-06-01 | 1995-03-28 | Miles Inc. | Device for the direct measurement of low density lipoprotein cholesterol |
| US5597532A (en) | 1994-10-20 | 1997-01-28 | Connolly; James | Apparatus for determining substances contained in a body fluid |
| JP2799835B2 (ja) | 1995-01-31 | 1998-09-21 | 第一化学薬品株式会社 | コレステロールの定量方法 |
| CN1148891A (zh) | 1995-03-20 | 1997-04-30 | 协和梅迪克斯株式会社 | 低密度脂蛋白中或极低密度脂蛋白中的胆甾醇的定量法 |
| JPH10160724A (ja) * | 1996-12-03 | 1998-06-19 | Kdk Corp | 試験片 |
| US6479249B2 (en) * | 1996-12-09 | 2002-11-12 | Denka Seiken Co., Ltd. | Method of determining cholesterol content of high-density lipoproteins |
| DE69737108T2 (de) | 1997-04-14 | 2007-07-05 | Denka Seiken Co., Ltd. | Methode zur quantifizierung von cholesterol in lipoproteinen geringer dichte |
| DE29715019U1 (de) | 1997-08-21 | 1997-10-16 | LRE Technology Partner GmbH, 80807 München | Teststreifen für ein Meßgerät zur optischen Bestimmung der Konzentration einer Substanz in einer Körperflüssigkeit |
| US6794157B1 (en) * | 1998-09-18 | 2004-09-21 | Kyowa Medex Co., Ltd. | Methods for fractional quatification of cholesterol in lipoproteins and quantification reagents |
| EP1183535A2 (en) | 1999-05-28 | 2002-03-06 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES | Homogeneous tests for sequentially determining lipoprotein fractions |
| DE19942928C2 (de) | 1999-09-08 | 2001-10-31 | Ernst Markart | Teststreifen |
| JP2001124780A (ja) * | 1999-10-28 | 2001-05-11 | Showa Denko Kk | リポ蛋白質コレステロールの測定方法 |
| JP2005526953A (ja) | 2001-09-17 | 2005-09-08 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | エンボス加工されたテストストリップシステム |
| US7214504B2 (en) | 2001-12-28 | 2007-05-08 | Polymer Technology Systems, Inc. | Test strip for determining concentration of triglycerides |
| DE10204606C1 (de) | 2002-01-18 | 2003-10-16 | Cholestech Corp | Vorrichtung und Verfahren für Lipoproteine hoher Dichte |
| ATE309544T1 (de) * | 2002-04-09 | 2005-11-15 | Cholestech Corp | Verfahren und vorrichtung zur quantifizierung von lipoprotein-cholesterol hoher dichte |
| US6759190B2 (en) | 2002-06-15 | 2004-07-06 | Acon Laboratories, Inc. | Test strip for detection of analyte and methods of use |
| US20040126830A1 (en) | 2002-09-16 | 2004-07-01 | Bruce Shull | Test strip and method for determining LDL cholesterol concentration from whole blood |
| US7435577B2 (en) | 2004-02-03 | 2008-10-14 | Polymer Technology Systems, Inc. | Direct measurement of chlolesterol from low density lipoprotein with test strip |
-
2004
- 2004-10-11 US US10/962,272 patent/US7435577B2/en not_active Expired - Lifetime
-
2005
- 2005-02-03 WO PCT/US2005/003234 patent/WO2005074609A2/en not_active Ceased
- 2005-02-03 BR BRPI0507386A patent/BRPI0507386B1/pt not_active IP Right Cessation
- 2005-02-03 EP EP05712615.3A patent/EP1725650B1/en not_active Expired - Lifetime
- 2005-02-03 CN CN201510349145.7A patent/CN104931712A/zh active Pending
- 2005-02-03 AU AU2005209856A patent/AU2005209856B2/en not_active Ceased
- 2005-02-03 EA EA200601415A patent/EA010414B1/ru not_active IP Right Cessation
- 2005-02-03 JP JP2006552209A patent/JP4785753B2/ja not_active Expired - Fee Related
- 2005-02-03 CA CA2554776A patent/CA2554776C/en not_active Expired - Lifetime
- 2005-02-03 PL PL05712615T patent/PL1725650T3/pl unknown
-
2006
- 2006-08-02 IL IL177226A patent/IL177226A/en not_active IP Right Cessation
- 2006-08-07 ZA ZA2006/06561A patent/ZA200606561B/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6342364B1 (en) * | 1999-11-22 | 2002-01-29 | Matsushita Electric Industrial Co., Ltd. | Cholesterol sensor and method of determining cholesterol |
| US20030092102A1 (en) * | 2000-01-31 | 2003-05-15 | Human Genome Sciences, Inc. | Nucleic acids, proteins, and antibodies |
| US20030003522A1 (en) * | 2001-06-29 | 2003-01-02 | International Business Machines Corporation | Method, system, and apparatus for measurement and recording of blood chemistry and other physiological measurements |
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0507386A (pt) | 2007-07-10 |
| JP2007523325A (ja) | 2007-08-16 |
| EA200601415A1 (ru) | 2006-12-29 |
| WO2005074609A3 (en) | 2006-01-19 |
| WO2005074609A2 (en) | 2005-08-18 |
| EP1725650A4 (en) | 2012-03-21 |
| IL177226A0 (en) | 2006-12-10 |
| IL177226A (en) | 2011-09-27 |
| BRPI0507386B1 (pt) | 2017-01-24 |
| US7435577B2 (en) | 2008-10-14 |
| CA2554776A1 (en) | 2005-08-18 |
| AU2005209856B2 (en) | 2010-06-10 |
| CA2554776C (en) | 2012-12-04 |
| ZA200606561B (en) | 2008-01-08 |
| EP1725650A2 (en) | 2006-11-29 |
| CN104931712A (zh) | 2015-09-23 |
| AU2005209856A1 (en) | 2005-08-18 |
| EP1725650B1 (en) | 2014-11-26 |
| PL1725650T3 (pl) | 2015-07-31 |
| US20050170447A1 (en) | 2005-08-04 |
| JP4785753B2 (ja) | 2011-10-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA010414B1 (ru) | Сочетание реагентов и способ непосредственного измерения при комнатной температуре содержания холестерина липопротеинов низкой плотности с использованием индикаторной полоски | |
| US5215886A (en) | HDL determination in whole blood | |
| US5213964A (en) | High-density lipoprotein solid-base precipitation assay method | |
| AU666821B2 (en) | Analyte detection device and process | |
| CN1168988C (zh) | 用于血液中葡萄糖测定的试剂测试条 | |
| EP2126587B1 (en) | Device and method for measuring ldl-associated cholesterol | |
| JPH0766001B2 (ja) | 一体化された分画工程を用いる高速診断器具によるｈｄｌコレステロールの測定方法 | |
| EP2116613B1 (en) | Method and test piece for measuring low density lipoprotein (ldl) cholesterol | |
| EP2108961B1 (en) | Dry analytical element for measurement of high density lipoprotein cholesterol | |
| JP4194992B2 (ja) | 抽出層を用いてアナライトを測定する方法 | |
| CN103562726B (zh) | 用于非空腹的ldl胆固醇检测的体系和方法 | |
| JPH0755810A (ja) | 被検体を検出する装置および方法 | |
| CN101001946B (zh) | 用于在环境温度下进行低密度脂蛋白胆固醇的直接测试条测量的试剂组合及方法 | |
| MXPA06008714A (en) | Reagent combination and method for direct test strip measurement of cholesterol from low density lipoproteins at ambient temperatures | |
| CN100569348C (zh) | 涂布膜的方法 | |
| JP2005257308A (ja) | 高比重リポ蛋白中コレステロール分析用乾式分析素子 | |
| HK1138367B (en) | Device and method for measuring ldl-associated cholesterol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG MD TJ TM RU |