AU2005209856B2 - Test strip composition and method to measure cholesterol from low density lipoproteins - Google Patents
Test strip composition and method to measure cholesterol from low density lipoproteins Download PDFInfo
- Publication number
- AU2005209856B2 AU2005209856B2 AU2005209856A AU2005209856A AU2005209856B2 AU 2005209856 B2 AU2005209856 B2 AU 2005209856B2 AU 2005209856 A AU2005209856 A AU 2005209856A AU 2005209856 A AU2005209856 A AU 2005209856A AU 2005209856 B2 AU2005209856 B2 AU 2005209856B2
- Authority
- AU
- Australia
- Prior art keywords
- test strip
- cholesterol
- membrane
- ldl
- layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims description 122
- 235000012000 cholesterol Nutrition 0.000 title claims description 61
- 238000012360 testing method Methods 0.000 title claims description 57
- 108010007622 LDL Lipoproteins Proteins 0.000 title claims description 43
- 102000007330 LDL Lipoproteins Human genes 0.000 title claims description 43
- 238000000034 method Methods 0.000 title description 21
- 239000000203 mixture Substances 0.000 title description 4
- 239000012528 membrane Substances 0.000 claims description 68
- 108010028554 LDL Cholesterol Proteins 0.000 claims description 40
- 229960000633 dextran sulfate Drugs 0.000 claims description 38
- 239000003153 chemical reaction reagent Substances 0.000 claims description 34
- 239000004094 surface-active agent Substances 0.000 claims description 26
- 210000004369 blood Anatomy 0.000 claims description 24
- 239000008280 blood Substances 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 21
- SPIUHJINJWTDQE-UHFFFAOYSA-N cyclohexaamylose polysulfate Chemical compound O1C(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC(C(OS(O)(=O)=O)C2OS(O)(=O)=O)C(COS(=O)(=O)O)OC2OC(C(C2OS(O)(=O)=O)OS(O)(=O)=O)C(COS(O)(=O)=O)OC2OC2C(OS(O)(=O)=O)C(OS(O)(=O)=O)C1OC2COS(O)(=O)=O SPIUHJINJWTDQE-UHFFFAOYSA-N 0.000 claims description 16
- 210000003743 erythrocyte Anatomy 0.000 claims description 15
- 102000004895 Lipoproteins Human genes 0.000 claims description 11
- 108090001030 Lipoproteins Proteins 0.000 claims description 11
- 229920001577 copolymer Polymers 0.000 claims description 11
- 210000002381 plasma Anatomy 0.000 claims description 11
- 210000002966 serum Anatomy 0.000 claims description 11
- 230000000903 blocking effect Effects 0.000 claims description 10
- -1 polyoxypropylene Polymers 0.000 claims description 10
- 229910052751 metal Inorganic materials 0.000 claims description 8
- 239000002184 metal Substances 0.000 claims description 8
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 6
- 229920000447 polyanionic polymer Polymers 0.000 claims description 6
- 150000001768 cations Chemical class 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 229920000642 polymer Polymers 0.000 claims description 5
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical class O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- 150000001412 amines Chemical class 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- 239000011777 magnesium Substances 0.000 claims description 4
- 229920001451 polypropylene glycol Polymers 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 1
- VFOSDIOKPAJPOS-UHFFFAOYSA-N hydroxysulfanylmethanamine Chemical group NCSO VFOSDIOKPAJPOS-UHFFFAOYSA-N 0.000 claims 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 108
- 239000000243 solution Substances 0.000 description 56
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 42
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 42
- 239000000523 sample Substances 0.000 description 42
- 229960002920 sorbitol Drugs 0.000 description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 41
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 40
- 239000000600 sorbitol Substances 0.000 description 33
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 32
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 31
- 229930006000 Sucrose Natural products 0.000 description 31
- 239000005720 sucrose Substances 0.000 description 31
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 30
- 229920002359 Tetronic® Polymers 0.000 description 26
- 239000008367 deionised water Substances 0.000 description 25
- 229910021641 deionized water Inorganic materials 0.000 description 25
- 239000007987 MES buffer Substances 0.000 description 22
- 238000003556 assay Methods 0.000 description 22
- HFHDHCJBZVLPGP-RWMJIURBSA-N alpha-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO HFHDHCJBZVLPGP-RWMJIURBSA-N 0.000 description 21
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 20
- 235000019341 magnesium sulphate Nutrition 0.000 description 20
- 239000007993 MOPS buffer Substances 0.000 description 19
- 238000006243 chemical reaction Methods 0.000 description 18
- 229910001629 magnesium chloride Inorganic materials 0.000 description 16
- 239000000126 substance Substances 0.000 description 15
- 239000000463 material Substances 0.000 description 13
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 12
- 238000005259 measurement Methods 0.000 description 8
- 230000007480 spreading Effects 0.000 description 8
- 238000003892 spreading Methods 0.000 description 8
- 229920000858 Cyclodextrin Polymers 0.000 description 7
- 108010023302 HDL Cholesterol Proteins 0.000 description 7
- 230000002255 enzymatic effect Effects 0.000 description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- 229920001983 poloxamer Polymers 0.000 description 6
- 239000004372 Polyvinyl alcohol Substances 0.000 description 5
- 125000002091 cationic group Chemical group 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 239000003365 glass fiber Substances 0.000 description 5
- 238000005470 impregnation Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 108010046315 IDL Lipoproteins Proteins 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 208000029078 coronary artery disease Diseases 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000000979 retarding effect Effects 0.000 description 4
- 108010089254 Cholesterol oxidase Proteins 0.000 description 3
- 108010010234 HDL Lipoproteins Proteins 0.000 description 3
- 102000015779 HDL Lipoproteins Human genes 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 108010055297 Sterol Esterase Proteins 0.000 description 3
- 102000000019 Sterol Esterase Human genes 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- COWYTPMAAISPHT-SWSWVKNJSA-A chembl411368 Chemical compound [K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].[K+].O1C(COS([O-])(=O)=O)[C@@H]2C(O)C(OS([O-])(=O)=O)[C@@H]1O[C@H](C(COS([O-])(=O)=O)O1)C(O)C(OS([O-])(=O)=O)[C@H]1O[C@H](C(COS([O-])(=O)=O)O1)C(O)C(OS([O-])(=O)=O)[C@H]1O[C@H](C(COS([O-])(=O)=O)O1)C(O)C(OS([O-])(=O)=O)[C@H]1O[C@H](C(COS([O-])(=O)=O)O1)C(O)C(OS([O-])(=O)=O)[C@H]1O[C@H](C(COS([O-])(=O)=O)O1)C(O)C(OS([O-])(=O)=O)[C@H]1O[C@H](C(COS([O-])(=O)=O)O1)C(O)C(OS([O-])(=O)=O)[C@H]1O2 COWYTPMAAISPHT-SWSWVKNJSA-A 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 229920000428 triblock copolymer Polymers 0.000 description 3
- 238000005199 ultracentrifugation Methods 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 108010004103 Chylomicrons Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000008214 LDL Cholesterol Methods 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 108010062497 VLDL Lipoproteins Proteins 0.000 description 2
- 229920001400 block copolymer Polymers 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 230000003292 diminished effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052748 manganese Inorganic materials 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000000693 micelle Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920002492 poly(sulfone) Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- FFJCNSLCJOQHKM-CLFAGFIQSA-N (z)-1-[(z)-octadec-9-enoxy]octadec-9-ene Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCCCCCCC\C=C/CCCCCCCC FFJCNSLCJOQHKM-CLFAGFIQSA-N 0.000 description 1
- JQRXTVACLHSVTF-UHFFFAOYSA-N 3-(2-methoxyethoxy)propyl-methyl-bis(trimethylsilyloxy)silane Chemical compound COCCOCCC[Si](C)(O[Si](C)(C)C)O[Si](C)(C)C JQRXTVACLHSVTF-UHFFFAOYSA-N 0.000 description 1
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
- GJMPSRSMBJLKKB-UHFFFAOYSA-N 3-methylphenylacetic acid Chemical compound CC1=CC=CC(CC(O)=O)=C1 GJMPSRSMBJLKKB-UHFFFAOYSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 229920006318 anionic polymer Polymers 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229960002086 dextran Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 1
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 108010022197 lipoprotein cholesterol Proteins 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 229940113115 polyethylene glycol 200 Drugs 0.000 description 1
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000005297 pyrex Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 229940117957 triethanolamine hydrochloride Drugs 0.000 description 1
- DSDAICPXUXPBCC-MWDJDSKUSA-N trimethyl-β-cyclodextrin Chemical compound COC[C@H]([C@H]([C@@H]([C@H]1OC)OC)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O[C@H]3O[C@H](COC)[C@H]([C@@H]([C@H]3OC)OC)O3)[C@H](OC)[C@H]2OC)COC)O[C@@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@@H]3O[C@@H]1COC DSDAICPXUXPBCC-MWDJDSKUSA-N 0.000 description 1
- 229960004418 trolamine Drugs 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000007704 wet chemistry method Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
- G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54168104P | 2004-02-03 | 2004-02-03 | |
| US60/541,681 | 2004-02-03 | ||
| US10/962,272 US7435577B2 (en) | 2004-02-03 | 2004-10-11 | Direct measurement of chlolesterol from low density lipoprotein with test strip |
| US10/962,272 | 2004-10-11 | ||
| PCT/US2005/003234 WO2005074609A2 (en) | 2004-02-03 | 2005-02-03 | Test strip composition and method to measure cholesterol from low density lipoproteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2005209856A1 AU2005209856A1 (en) | 2005-08-18 |
| AU2005209856B2 true AU2005209856B2 (en) | 2010-06-10 |
Family
ID=34811458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2005209856A Ceased AU2005209856B2 (en) | 2004-02-03 | 2005-02-03 | Test strip composition and method to measure cholesterol from low density lipoproteins |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7435577B2 (enExample) |
| EP (1) | EP1725650B1 (enExample) |
| JP (1) | JP4785753B2 (enExample) |
| CN (1) | CN104931712A (enExample) |
| AU (1) | AU2005209856B2 (enExample) |
| BR (1) | BRPI0507386B1 (enExample) |
| CA (1) | CA2554776C (enExample) |
| EA (1) | EA010414B1 (enExample) |
| IL (1) | IL177226A (enExample) |
| PL (1) | PL1725650T3 (enExample) |
| WO (1) | WO2005074609A2 (enExample) |
| ZA (1) | ZA200606561B (enExample) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7625721B2 (en) | 2004-02-03 | 2009-12-01 | Polymer Technology Systems, Inc. | Non-precipitating bodily fluid analysis system |
| US8465696B2 (en) * | 2004-02-03 | 2013-06-18 | Polymer Technology Systems, Inc. | Dry test strip with controlled flow and method of manufacturing same |
| US7435577B2 (en) | 2004-02-03 | 2008-10-14 | Polymer Technology Systems, Inc. | Direct measurement of chlolesterol from low density lipoprotein with test strip |
| US20060062688A1 (en) * | 2004-02-03 | 2006-03-23 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| US7491542B2 (en) * | 2004-07-12 | 2009-02-17 | Kim Scheuringer | Test device for determining the concentration of LDL-cholesterol in a sample |
| PL1779119T3 (pl) * | 2004-08-17 | 2012-12-31 | Polymer Technology Systems Inc | Sposób bezstrąceniowej analizy płynów ustrojowych |
| US20060062690A1 (en) * | 2004-08-17 | 2006-03-23 | Polymer Technology Systems, Inc. | Apparatus and method of manufacturing bodily fluid test strip |
| JP5247158B2 (ja) * | 2008-01-16 | 2013-07-24 | パナソニック株式会社 | 試料溶液分析方法および試料溶液分析装置 |
| WO2009155339A1 (en) * | 2008-06-17 | 2009-12-23 | Polymer Technology Systems, Inc. | System and method for packaging dry test strips |
| CN102209789B (zh) * | 2008-11-14 | 2015-07-22 | 协和梅迪克斯株式会社 | 低密度脂蛋白中的胆固醇的测定方法、测定用试剂及测定用试剂盒 |
| KR101058743B1 (ko) * | 2009-06-04 | 2011-08-24 | 주식회사 인포피아 | 콜레스테롤 측정 스트립 및 이를 이용한 콜레스테롤 검출 방법 |
| KR101203385B1 (ko) * | 2009-06-04 | 2012-11-21 | 주식회사 인포피아 | 혈액의 퍼짐성이 향상된 측정 스트립 |
| KR101110561B1 (ko) * | 2009-06-04 | 2012-02-15 | 주식회사 인포피아 | 바이오 센서 |
| JP5460183B2 (ja) * | 2009-08-28 | 2014-04-02 | パナソニック株式会社 | 分析用試薬および分析用デバイス |
| WO2011007514A1 (ja) * | 2009-07-15 | 2011-01-20 | パナソニック株式会社 | 分析用試薬およびこれを担持した分析用デバイス |
| US8012770B2 (en) | 2009-07-31 | 2011-09-06 | Invisible Sentinel, Inc. | Device for detection of antigens and uses thereof |
| ES2461992T3 (es) | 2009-10-09 | 2014-05-22 | Invisible Sentinel, Inc. | Dispositivo para la detección de antígenos y usos de los mismos |
| JP5285000B2 (ja) * | 2010-02-23 | 2013-09-11 | 富士フイルム株式会社 | 低密度リポ蛋白コレステロールの測定方法 |
| WO2011136316A1 (ja) * | 2010-04-30 | 2011-11-03 | 協和メデックス株式会社 | 低密度リポ蛋白中のコレステロールの測定方法、測定用試薬及び測定用キット |
| WO2012103511A2 (en) | 2011-01-27 | 2012-08-02 | Invisible Sentinel, Inc. | Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof |
| GB2503601A (en) * | 2011-04-22 | 2014-01-01 | Polymer Technology Systems Inc | Blood Separation system and method for a dry test strip |
| WO2013134503A2 (en) | 2012-03-09 | 2013-09-12 | Invisible Sentinel, Inc. | Methods And Compositions For Detecting Multiple Analytes With A Single Signal |
| DE112013002316T5 (de) * | 2012-05-04 | 2015-04-23 | Polymer Technology Systems, Inc. | Systeme und Verfahren für Assays von im nicht-nüchternen Zustand gewonnenem LDL-Cholesterin |
| KR102630880B1 (ko) | 2014-03-07 | 2024-01-29 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 분석물 추출, 농축 및 검출을 통합하기 위한 장치 |
| AU2015283689B2 (en) * | 2014-07-03 | 2020-07-16 | Abionic Sa | Capsule for rapid molecular quantification of a fluid sample such as whole blood |
| MA41883B1 (fr) * | 2015-07-23 | 2019-03-29 | Gentian Diagnostics As | Procédé pour évaluer les récepteurs de surface cellulaire des cellules sanguines |
| WO2017041030A1 (en) | 2015-09-04 | 2017-03-09 | The Regents Of The University Of California | Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications |
| EP4202439B1 (en) | 2016-06-09 | 2025-02-19 | The Regents of the University of California | Method of purifying and amplifying a nucleic acid |
| US11327075B2 (en) | 2016-08-22 | 2022-05-10 | The Regents Of The University Of California | Hydrogel platform for aqueous two-phase concentration of a target to enhance its detection |
| WO2018138139A1 (en) * | 2017-01-24 | 2018-08-02 | Gentian As | Method for assessing cell surface receptors of blood cells |
| US11209427B2 (en) | 2017-03-27 | 2021-12-28 | The Regents Of The University Of California | Semi-quantitative lateral-flow immunoassay for the detection of CSF leaks |
| US11338294B2 (en) | 2017-04-27 | 2022-05-24 | Polybiomics, Inc. | Orthogonal polybiosensing and imaging systems |
| US12168795B2 (en) | 2017-09-01 | 2024-12-17 | Hitachi Chemical Diagnostics Systems Co., Ltd. | Method for measuring cholesterol in low-density lipoprotein, measurement reagent, and measurement kit |
| CN107402209B (zh) * | 2017-09-15 | 2020-04-07 | 光景生物科技(苏州)有限公司 | 一种用于检测血清中低密度脂蛋白胆固醇的试纸条及其制备方法 |
| JP6454950B1 (ja) * | 2018-03-29 | 2019-01-23 | 株式会社明日香特殊検査研究所 | 粥状動脈硬化による心筋梗塞や脳梗塞のリスクが高いと判定する易酸化性ＶＬＤＬ（ＶＬＤＬ ｓｕｓｃｅｐｔｉｂｉｌｉｔｙ ｔｏ ｏｘｉｄａｔｉｏｎ）および易酸化性ＬＤＬ（ＬＤＬ ｓｕｓｃｅｐｔｉｂｉｌｉｔｙ ｔｏ ｏｘｉｄａｔｉｏｎ）の簡便な測定方法および測定装置 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6342364B1 (en) * | 1999-11-22 | 2002-01-29 | Matsushita Electric Industrial Co., Ltd. | Cholesterol sensor and method of determining cholesterol |
| US20030003522A1 (en) * | 2001-06-29 | 2003-01-02 | International Business Machines Corporation | Method, system, and apparatus for measurement and recording of blood chemistry and other physiological measurements |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3809617A (en) | 1972-11-15 | 1974-05-07 | American Cyanamid Co | Device for detecting anticholinesterase materials |
| US4178153A (en) | 1977-11-21 | 1979-12-11 | Damon Corporation | Method and apparatus for chemical spot test analysis |
| DE3029579C2 (de) | 1980-08-05 | 1985-12-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Mittel zur Abtrennung von Plasma oder Serum aus Vollblut |
| DE3217925A1 (de) | 1982-05-13 | 1983-11-17 | B. Braun Melsungen Ag, 3508 Melsungen | Verfahren zur selektiven extrakorporalen praezipitation von low-density lipoproteinen aus vollserum oder plasma fuer diagnostische zwecke |
| DE3401932A1 (de) | 1984-01-20 | 1985-08-01 | Collmann GmbH & Co, Spezialmaschinenbau KG, 2400 Lübeck | Verfahren und vorrichtung zum darbieten von verstaerkungseinlagen fuer schleifscheiben |
| DE3530993A1 (de) | 1985-08-30 | 1987-03-05 | Miles Lab | Teststreifen mit festlegbarer probenaufnahmekapazitaet |
| TW203120B (enExample) | 1985-10-04 | 1993-04-01 | Abbott Lab | |
| DE3782597T3 (de) | 1986-09-18 | 2002-09-05 | Pacific Biotech Inc., San Diego | Immunodiagnostische Vorrichtung. |
| EP0269876B1 (en) | 1986-11-24 | 1993-08-18 | Abbott Laboratories | Sample focuser for solid-phase analytical device |
| US4774192A (en) | 1987-01-28 | 1988-09-27 | Technimed Corporation | A dry reagent delivery system with membrane having porosity gradient |
| JPH0264455A (ja) | 1988-08-31 | 1990-03-05 | Konica Corp | 体液検査素子及びその製造方法 |
| US5135716A (en) | 1989-07-12 | 1992-08-04 | Kingston Diagnostics, L.P. | Direct measurement of HDL cholesterol via dry chemistry strips |
| DE3929032C2 (de) | 1989-09-01 | 1998-09-03 | Boehringer Mannheim Gmbh | Verfahren zur Bestimmung von HDL-Cholesterin mittels eines Schnelldiagnostikums mit integriertem Fraktionierschritt |
| AU634814B2 (en) | 1989-09-14 | 1993-03-04 | Terumo Kabushiki Kaisha | Method for determination of ion concentration, specific gravity, or osmotic pressure of solution and apparatus therefor |
| JPH04324347A (ja) | 1991-04-24 | 1992-11-13 | Terumo Corp | 試験具 |
| GR1001410B (el) * | 1991-08-02 | 1993-11-30 | Ortho Pharma Corp | Επινόημα & μέ?οδος διεξαγωγής βιολογικών δοκιμασιών περιέχοντας σύστημα μοριακής διαλογής. |
| US5286626A (en) * | 1991-12-13 | 1994-02-15 | Actimed Laboratories, Inc. | Process and apparatus for direct determination of low density lipoprotein |
| US5460974A (en) | 1992-10-13 | 1995-10-24 | Miles Inc. | Method of assaying whole blood for HDL cholesterol |
| US5401466A (en) * | 1993-06-01 | 1995-03-28 | Miles Inc. | Device for the direct measurement of low density lipoprotein cholesterol |
| US5597532A (en) | 1994-10-20 | 1997-01-28 | Connolly; James | Apparatus for determining substances contained in a body fluid |
| JP2799835B2 (ja) | 1995-01-31 | 1998-09-21 | 第一化学薬品株式会社 | コレステロールの定量方法 |
| AU702445B2 (en) | 1995-03-20 | 1999-02-18 | Kyowa Medex Co., Ltd. | Method for determination of cholesterol in low-density lipoprotein or very low-density lipoprotein |
| JPH10160724A (ja) * | 1996-12-03 | 1998-06-19 | Kdk Corp | 試験片 |
| DE69734194T2 (de) * | 1996-12-09 | 2006-06-29 | Denka Seiken Co., Ltd. | Verfahren zur bestimmung des cholesteringehalts von high-density-lipoproteinen |
| ES2278395T3 (es) | 1997-04-14 | 2007-08-01 | Denka Seiken Co., Ltd. | Procedimiento para cuantificar el colesterol en lipoproteinas de baja densidad. |
| DE29715019U1 (de) | 1997-08-21 | 1997-10-16 | LRE Technology Partner GmbH, 80807 München | Teststreifen für ein Meßgerät zur optischen Bestimmung der Konzentration einer Substanz in einer Körperflüssigkeit |
| EP1114870B1 (en) * | 1998-09-18 | 2004-10-06 | Kyowa Medex Co., Ltd. | Methods for fractional quantification of cholesterol in lipoproteins and quantification reagents |
| EP1183535A2 (en) | 1999-05-28 | 2002-03-06 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES | Homogeneous tests for sequentially determining lipoprotein fractions |
| DE19942928C2 (de) | 1999-09-08 | 2001-10-31 | Ernst Markart | Teststreifen |
| JP2001124780A (ja) * | 1999-10-28 | 2001-05-11 | Showa Denko Kk | リポ蛋白質コレステロールの測定方法 |
| US20030013649A1 (en) | 2000-01-31 | 2003-01-16 | Rosen Craig A. | Nucleic acids, proteins, and antibodies |
| CA2460073A1 (en) | 2001-09-17 | 2003-03-27 | F. Hoffmann-La Roche Ag | Embossed test strip system |
| US7494818B1 (en) | 2001-12-28 | 2009-02-24 | Polymer Technology Systems, Inc. | Method for determining concentration of multiple analytes in a single fluid sample |
| DE10204606C1 (de) | 2002-01-18 | 2003-10-16 | Cholestech Corp | Vorrichtung und Verfahren für Lipoproteine hoher Dichte |
| EP1357383B1 (en) * | 2002-04-09 | 2005-11-09 | Cholestech Corporation | High-density lipoprotein assay device and method |
| US6759190B2 (en) | 2002-06-15 | 2004-07-06 | Acon Laboratories, Inc. | Test strip for detection of analyte and methods of use |
| US20040126830A1 (en) | 2002-09-16 | 2004-07-01 | Bruce Shull | Test strip and method for determining LDL cholesterol concentration from whole blood |
| US7435577B2 (en) | 2004-02-03 | 2008-10-14 | Polymer Technology Systems, Inc. | Direct measurement of chlolesterol from low density lipoprotein with test strip |
-
2004
- 2004-10-11 US US10/962,272 patent/US7435577B2/en not_active Expired - Lifetime
-
2005
- 2005-02-03 CA CA2554776A patent/CA2554776C/en not_active Expired - Lifetime
- 2005-02-03 JP JP2006552209A patent/JP4785753B2/ja not_active Expired - Fee Related
- 2005-02-03 AU AU2005209856A patent/AU2005209856B2/en not_active Ceased
- 2005-02-03 EA EA200601415A patent/EA010414B1/ru not_active IP Right Cessation
- 2005-02-03 PL PL05712615T patent/PL1725650T3/pl unknown
- 2005-02-03 EP EP05712615.3A patent/EP1725650B1/en not_active Expired - Lifetime
- 2005-02-03 BR BRPI0507386A patent/BRPI0507386B1/pt not_active IP Right Cessation
- 2005-02-03 CN CN201510349145.7A patent/CN104931712A/zh active Pending
- 2005-02-03 WO PCT/US2005/003234 patent/WO2005074609A2/en not_active Ceased
-
2006
- 2006-08-02 IL IL177226A patent/IL177226A/en not_active IP Right Cessation
- 2006-08-07 ZA ZA2006/06561A patent/ZA200606561B/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6342364B1 (en) * | 1999-11-22 | 2002-01-29 | Matsushita Electric Industrial Co., Ltd. | Cholesterol sensor and method of determining cholesterol |
| US20030003522A1 (en) * | 2001-06-29 | 2003-01-02 | International Business Machines Corporation | Method, system, and apparatus for measurement and recording of blood chemistry and other physiological measurements |
Non-Patent Citations (2)
| Title |
|---|
| NAUCK, M. et al., Clin Chem., 2002, vol 48, pages 236-254 * |
| SANTEE, J., Am. J. Health-Sys. Pharm, 2002, vol. 59, pages 1774-1779 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2005209856A1 (en) | 2005-08-18 |
| EP1725650A2 (en) | 2006-11-29 |
| CA2554776C (en) | 2012-12-04 |
| EP1725650B1 (en) | 2014-11-26 |
| CN104931712A (zh) | 2015-09-23 |
| EA010414B1 (ru) | 2008-08-29 |
| IL177226A (en) | 2011-09-27 |
| BRPI0507386A (pt) | 2007-07-10 |
| CA2554776A1 (en) | 2005-08-18 |
| WO2005074609A2 (en) | 2005-08-18 |
| JP4785753B2 (ja) | 2011-10-05 |
| PL1725650T3 (pl) | 2015-07-31 |
| JP2007523325A (ja) | 2007-08-16 |
| WO2005074609A3 (en) | 2006-01-19 |
| BRPI0507386B1 (pt) | 2017-01-24 |
| US7435577B2 (en) | 2008-10-14 |
| EA200601415A1 (ru) | 2006-12-29 |
| EP1725650A4 (en) | 2012-03-21 |
| IL177226A0 (en) | 2006-12-10 |
| ZA200606561B (en) | 2008-01-08 |
| US20050170447A1 (en) | 2005-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2005209856B2 (en) | Test strip composition and method to measure cholesterol from low density lipoproteins | |
| JP2981774B2 (ja) | 固相沈澱アッセイ装置および方法 | |
| JP3437647B2 (ja) | 被検体を検出する装置および方法 | |
| US5215886A (en) | HDL determination in whole blood | |
| US7824879B2 (en) | Device and method for measuring LDL-associated cholesterol | |
| KR20090125794A (ko) | 소립자 저비중 리포 단백의 정량 시약 | |
| US8304204B2 (en) | Method for measuring low-density lipoprotein (LDL) cholesterol | |
| EP2108961B1 (en) | Dry analytical element for measurement of high density lipoprotein cholesterol | |
| JP4261369B2 (ja) | 高密度リポタンパク質アッセイデバイスおよび方法 | |
| JP2006521823A (ja) | 高密度リポタンパク質に関連する血清コレステロールを測定するためのアッセイデバイス中の多孔性および生物学的活性を保持する接着膜 | |
| EP2922964A2 (en) | Systems and methods for monitoring biological fluids | |
| JP3439836B2 (ja) | 被検体を検出する装置および方法 | |
| CA2573231A1 (en) | Test device for ldl-cholesterol | |
| JP4889670B2 (ja) | 溶血の影響を低減した体液成分測定用乾式分析素子 | |
| CN101001946B (zh) | 用于在环境温度下进行低密度脂蛋白胆固醇的直接测试条测量的试剂组合及方法 | |
| CN115436615A (zh) | 一种总胆固醇检测用干式分析试剂 | |
| MXPA06008714A (en) | Reagent combination and method for direct test strip measurement of cholesterol from low density lipoproteins at ambient temperatures | |
| JP4879185B2 (ja) | 膜のコーティング方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTION TITLE TO READ TEST STRIP COMPOSITION AND METHOD TO MEASURE CHOLESTEROL FROM LOW DENSITY LIPOPROTEINS |
|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |