JP4785753B2 - 低密度リポプロテインからのコレステロールの室温での直接テストストリップ測定のための試薬組み合わせ、および方法 - Google Patents
低密度リポプロテインからのコレステロールの室温での直接テストストリップ測定のための試薬組み合わせ、および方法 Download PDFInfo
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- JP4785753B2 JP4785753B2 JP2006552209A JP2006552209A JP4785753B2 JP 4785753 B2 JP4785753 B2 JP 4785753B2 JP 2006552209 A JP2006552209 A JP 2006552209A JP 2006552209 A JP2006552209 A JP 2006552209A JP 4785753 B2 JP4785753 B2 JP 4785753B2
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- test strip
- cholesterol
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Classifications
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
- G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
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- Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
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Applications Claiming Priority (5)
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| US60/541,681 | 2004-02-03 | ||
| US10/962,272 US7435577B2 (en) | 2004-02-03 | 2004-10-11 | Direct measurement of chlolesterol from low density lipoprotein with test strip |
| US10/962,272 | 2004-10-11 | ||
| PCT/US2005/003234 WO2005074609A2 (en) | 2004-02-03 | 2005-02-03 | Test strip composition and method to measure cholesterol from low density lipoproteins |
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| JP2007523325A JP2007523325A (ja) | 2007-08-16 |
| JP2007523325A5 JP2007523325A5 (enExample) | 2008-01-24 |
| JP4785753B2 true JP4785753B2 (ja) | 2011-10-05 |
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| EP (1) | EP1725650B1 (enExample) |
| JP (1) | JP4785753B2 (enExample) |
| CN (1) | CN104931712A (enExample) |
| AU (1) | AU2005209856B2 (enExample) |
| BR (1) | BRPI0507386B1 (enExample) |
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| PL (1) | PL1725650T3 (enExample) |
| WO (1) | WO2005074609A2 (enExample) |
| ZA (1) | ZA200606561B (enExample) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060062688A1 (en) * | 2004-02-03 | 2006-03-23 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| US7625721B2 (en) * | 2004-02-03 | 2009-12-01 | Polymer Technology Systems, Inc. | Non-precipitating bodily fluid analysis system |
| US8465696B2 (en) * | 2004-02-03 | 2013-06-18 | Polymer Technology Systems, Inc. | Dry test strip with controlled flow and method of manufacturing same |
| US7435577B2 (en) | 2004-02-03 | 2008-10-14 | Polymer Technology Systems, Inc. | Direct measurement of chlolesterol from low density lipoprotein with test strip |
| US7491542B2 (en) * | 2004-07-12 | 2009-02-17 | Kim Scheuringer | Test device for determining the concentration of LDL-cholesterol in a sample |
| US20060062690A1 (en) * | 2004-08-17 | 2006-03-23 | Polymer Technology Systems, Inc. | Apparatus and method of manufacturing bodily fluid test strip |
| WO2006033733A1 (en) * | 2004-08-17 | 2006-03-30 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| JP5247158B2 (ja) * | 2008-01-16 | 2013-07-24 | パナソニック株式会社 | 試料溶液分析方法および試料溶液分析装置 |
| US20110200498A1 (en) * | 2008-06-17 | 2011-08-18 | Polymer Technology Systems, Inc. | System and method for packaging dry test strips |
| US8685663B2 (en) * | 2008-11-14 | 2014-04-01 | Kyowa Medex Co., Ltd. | Method, reagent and kit for measuring cholesterol in low-density lipoproteins |
| KR101058743B1 (ko) * | 2009-06-04 | 2011-08-24 | 주식회사 인포피아 | 콜레스테롤 측정 스트립 및 이를 이용한 콜레스테롤 검출 방법 |
| KR101110561B1 (ko) * | 2009-06-04 | 2012-02-15 | 주식회사 인포피아 | 바이오 센서 |
| KR101203385B1 (ko) * | 2009-06-04 | 2012-11-21 | 주식회사 인포피아 | 혈액의 퍼짐성이 향상된 측정 스트립 |
| JP5460183B2 (ja) * | 2009-08-28 | 2014-04-02 | パナソニック株式会社 | 分析用試薬および分析用デバイス |
| US20120107850A1 (en) * | 2009-07-15 | 2012-05-03 | Panasonic Corporation | Analysis reagent and analysis device having the analysis reagent carried therein |
| US8012770B2 (en) | 2009-07-31 | 2011-09-06 | Invisible Sentinel, Inc. | Device for detection of antigens and uses thereof |
| WO2011044574A1 (en) | 2009-10-09 | 2011-04-14 | Invisible Sentinel | Device for detection of antigens and uses thereof |
| JP5285000B2 (ja) * | 2010-02-23 | 2013-09-11 | 富士フイルム株式会社 | 低密度リポ蛋白コレステロールの測定方法 |
| JP5832995B2 (ja) * | 2010-04-30 | 2015-12-16 | 協和メデックス株式会社 | 低密度リポ蛋白中のコレステロールの測定方法、測定用試薬及び測定用キット |
| AU2012211141B2 (en) | 2011-01-27 | 2016-11-03 | Invisible Sentinel, Inc. | Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof |
| JP6018177B2 (ja) * | 2011-04-22 | 2016-11-02 | ポリマー テクノロジー システムズ インコーポレーテッド | 乾燥試験ストリップのための血液分離システムおよび方法 |
| CA2866379C (en) | 2012-03-09 | 2020-10-13 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| DE112013002316T5 (de) * | 2012-05-04 | 2015-04-23 | Polymer Technology Systems, Inc. | Systeme und Verfahren für Assays von im nicht-nüchternen Zustand gewonnenem LDL-Cholesterin |
| EP3114482B1 (en) | 2014-03-07 | 2020-10-07 | The Regents of the University of California | Devices for integrating analyte extraction, concentration and detection |
| PT3164213T (pt) * | 2014-07-03 | 2024-03-25 | Abionic Sa | Cápsula para quantificação molecular rápida de uma amostra de fluido tal como sangue total |
| BR112018001256A2 (pt) * | 2015-07-23 | 2018-09-11 | Gentian Diagnostics As | método de ensaio para avaliação de uma ou mais subclasses de células sanguíneas de interesse (bcoi), método para avaliar a quantidade de receptores de cd4 localizados nas superfícies de células cd4+ e opcionalmente, para avaliar a quantidade de receptores de cd8 localizados nas superfícies de células, dispositivo de ensaio de fluxo vertical para realizar um método, e, uso de um dispositivo. |
| EP3345001A4 (en) | 2015-09-04 | 2019-07-17 | The Regents of the University of California | METHOD AND DEVICES FOR ANALYTICAL COLLECTION, EXTRACTION, CONCENTRATION AND DETECTION FOR CLINICAL APPLICATIONS |
| EP4202439B1 (en) | 2016-06-09 | 2025-02-19 | The Regents of the University of California | Method of purifying and amplifying a nucleic acid |
| WO2018039139A1 (en) | 2016-08-22 | 2018-03-01 | The Regents Of The University Of California | Hydrogel platform for aqueous two-phase concentration of a target to enhance its detection |
| WO2018138139A1 (en) * | 2017-01-24 | 2018-08-02 | Gentian As | Method for assessing cell surface receptors of blood cells |
| US11209427B2 (en) | 2017-03-27 | 2021-12-28 | The Regents Of The University Of California | Semi-quantitative lateral-flow immunoassay for the detection of CSF leaks |
| US11338294B2 (en) | 2017-04-27 | 2022-05-24 | Polybiomics, Inc. | Orthogonal polybiosensing and imaging systems |
| EP3677688B1 (en) | 2017-09-01 | 2024-08-07 | Hitachi Chemical Diagnostics Systems Co., Ltd. | Method for measuring cholesterol in low-density lipoprotein, use of a measurement reagent, and of a measurement kit |
| CN107402209B (zh) * | 2017-09-15 | 2020-04-07 | 光景生物科技(苏州)有限公司 | 一种用于检测血清中低密度脂蛋白胆固醇的试纸条及其制备方法 |
| JP6454950B1 (ja) * | 2018-03-29 | 2019-01-23 | 株式会社明日香特殊検査研究所 | 粥状動脈硬化による心筋梗塞や脳梗塞のリスクが高いと判定する易酸化性ＶＬＤＬ（ＶＬＤＬ ｓｕｓｃｅｐｔｉｂｉｌｉｔｙ ｔｏ ｏｘｉｄａｔｉｏｎ）および易酸化性ＬＤＬ（ＬＤＬ ｓｕｓｃｅｐｔｉｂｉｌｉｔｙ ｔｏ ｏｘｉｄａｔｉｏｎ）の簡便な測定方法および測定装置 |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3809617A (en) | 1972-11-15 | 1974-05-07 | American Cyanamid Co | Device for detecting anticholinesterase materials |
| US4178153A (en) | 1977-11-21 | 1979-12-11 | Damon Corporation | Method and apparatus for chemical spot test analysis |
| DE3029579C2 (de) | 1980-08-05 | 1985-12-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Mittel zur Abtrennung von Plasma oder Serum aus Vollblut |
| DE3217925A1 (de) | 1982-05-13 | 1983-11-17 | B. Braun Melsungen Ag, 3508 Melsungen | Verfahren zur selektiven extrakorporalen praezipitation von low-density lipoproteinen aus vollserum oder plasma fuer diagnostische zwecke |
| DE3401932A1 (de) | 1984-01-20 | 1985-08-01 | Collmann GmbH & Co, Spezialmaschinenbau KG, 2400 Lübeck | Verfahren und vorrichtung zum darbieten von verstaerkungseinlagen fuer schleifscheiben |
| DE3530993A1 (de) | 1985-08-30 | 1987-03-05 | Miles Lab | Teststreifen mit festlegbarer probenaufnahmekapazitaet |
| TW203120B (enExample) | 1985-10-04 | 1993-04-01 | Abbott Lab | |
| DE3782597T3 (de) | 1986-09-18 | 2002-09-05 | Pacific Biotech Inc., San Diego | Immunodiagnostische Vorrichtung. |
| DE3787078T2 (de) | 1986-11-24 | 1993-12-09 | Abbott Lab | Proberichtwirkung für analytisches Festphasengerät. |
| US4774192A (en) | 1987-01-28 | 1988-09-27 | Technimed Corporation | A dry reagent delivery system with membrane having porosity gradient |
| JPH0264455A (ja) | 1988-08-31 | 1990-03-05 | Konica Corp | 体液検査素子及びその製造方法 |
| US5135716A (en) | 1989-07-12 | 1992-08-04 | Kingston Diagnostics, L.P. | Direct measurement of HDL cholesterol via dry chemistry strips |
| DE3929032C2 (de) | 1989-09-01 | 1998-09-03 | Boehringer Mannheim Gmbh | Verfahren zur Bestimmung von HDL-Cholesterin mittels eines Schnelldiagnostikums mit integriertem Fraktionierschritt |
| AU634814B2 (en) | 1989-09-14 | 1993-03-04 | Terumo Kabushiki Kaisha | Method for determination of ion concentration, specific gravity, or osmotic pressure of solution and apparatus therefor |
| JPH04324347A (ja) | 1991-04-24 | 1992-11-13 | Terumo Corp | 試験具 |
| GR1001410B (el) * | 1991-08-02 | 1993-11-30 | Ortho Pharma Corp | Επινόημα & μέ?οδος διεξαγωγής βιολογικών δοκιμασιών περιέχοντας σύστημα μοριακής διαλογής. |
| US5286626A (en) * | 1991-12-13 | 1994-02-15 | Actimed Laboratories, Inc. | Process and apparatus for direct determination of low density lipoprotein |
| US5460974A (en) | 1992-10-13 | 1995-10-24 | Miles Inc. | Method of assaying whole blood for HDL cholesterol |
| US5401466A (en) * | 1993-06-01 | 1995-03-28 | Miles Inc. | Device for the direct measurement of low density lipoprotein cholesterol |
| US5597532A (en) | 1994-10-20 | 1997-01-28 | Connolly; James | Apparatus for determining substances contained in a body fluid |
| JP2799835B2 (ja) | 1995-01-31 | 1998-09-21 | 第一化学薬品株式会社 | コレステロールの定量方法 |
| CN1148891A (zh) | 1995-03-20 | 1997-04-30 | 协和梅迪克斯株式会社 | 低密度脂蛋白中或极低密度脂蛋白中的胆甾醇的定量法 |
| JPH10160724A (ja) * | 1996-12-03 | 1998-06-19 | Kdk Corp | 試験片 |
| US6479249B2 (en) * | 1996-12-09 | 2002-11-12 | Denka Seiken Co., Ltd. | Method of determining cholesterol content of high-density lipoproteins |
| DE69737108T2 (de) | 1997-04-14 | 2007-07-05 | Denka Seiken Co., Ltd. | Methode zur quantifizierung von cholesterol in lipoproteinen geringer dichte |
| DE29715019U1 (de) | 1997-08-21 | 1997-10-16 | LRE Technology Partner GmbH, 80807 München | Teststreifen für ein Meßgerät zur optischen Bestimmung der Konzentration einer Substanz in einer Körperflüssigkeit |
| US6794157B1 (en) * | 1998-09-18 | 2004-09-21 | Kyowa Medex Co., Ltd. | Methods for fractional quatification of cholesterol in lipoproteins and quantification reagents |
| EP1183535A2 (en) | 1999-05-28 | 2002-03-06 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES | Homogeneous tests for sequentially determining lipoprotein fractions |
| DE19942928C2 (de) | 1999-09-08 | 2001-10-31 | Ernst Markart | Teststreifen |
| JP2001124780A (ja) * | 1999-10-28 | 2001-05-11 | Showa Denko Kk | リポ蛋白質コレステロールの測定方法 |
| US6342364B1 (en) | 1999-11-22 | 2002-01-29 | Matsushita Electric Industrial Co., Ltd. | Cholesterol sensor and method of determining cholesterol |
| US20030013649A1 (en) | 2000-01-31 | 2003-01-16 | Rosen Craig A. | Nucleic acids, proteins, and antibodies |
| US6844149B2 (en) | 2001-06-29 | 2005-01-18 | International Business Machines Corporation | Method, system, and apparatus for measurement and recording of blood chemistry and other physiological measurements |
| JP2005526953A (ja) | 2001-09-17 | 2005-09-08 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | エンボス加工されたテストストリップシステム |
| US7214504B2 (en) | 2001-12-28 | 2007-05-08 | Polymer Technology Systems, Inc. | Test strip for determining concentration of triglycerides |
| DE10204606C1 (de) | 2002-01-18 | 2003-10-16 | Cholestech Corp | Vorrichtung und Verfahren für Lipoproteine hoher Dichte |
| ATE309544T1 (de) * | 2002-04-09 | 2005-11-15 | Cholestech Corp | Verfahren und vorrichtung zur quantifizierung von lipoprotein-cholesterol hoher dichte |
| US6759190B2 (en) | 2002-06-15 | 2004-07-06 | Acon Laboratories, Inc. | Test strip for detection of analyte and methods of use |
| US20040126830A1 (en) | 2002-09-16 | 2004-07-01 | Bruce Shull | Test strip and method for determining LDL cholesterol concentration from whole blood |
| US7435577B2 (en) | 2004-02-03 | 2008-10-14 | Polymer Technology Systems, Inc. | Direct measurement of chlolesterol from low density lipoprotein with test strip |
-
2004
- 2004-10-11 US US10/962,272 patent/US7435577B2/en not_active Expired - Lifetime
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2005
- 2005-02-03 WO PCT/US2005/003234 patent/WO2005074609A2/en not_active Ceased
- 2005-02-03 BR BRPI0507386A patent/BRPI0507386B1/pt not_active IP Right Cessation
- 2005-02-03 EP EP05712615.3A patent/EP1725650B1/en not_active Expired - Lifetime
- 2005-02-03 CN CN201510349145.7A patent/CN104931712A/zh active Pending
- 2005-02-03 AU AU2005209856A patent/AU2005209856B2/en not_active Ceased
- 2005-02-03 EA EA200601415A patent/EA010414B1/ru not_active IP Right Cessation
- 2005-02-03 JP JP2006552209A patent/JP4785753B2/ja not_active Expired - Fee Related
- 2005-02-03 CA CA2554776A patent/CA2554776C/en not_active Expired - Lifetime
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Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0507386A (pt) | 2007-07-10 |
| JP2007523325A (ja) | 2007-08-16 |
| EA200601415A1 (ru) | 2006-12-29 |
| WO2005074609A3 (en) | 2006-01-19 |
| WO2005074609A2 (en) | 2005-08-18 |
| EP1725650A4 (en) | 2012-03-21 |
| IL177226A0 (en) | 2006-12-10 |
| IL177226A (en) | 2011-09-27 |
| EA010414B1 (ru) | 2008-08-29 |
| BRPI0507386B1 (pt) | 2017-01-24 |
| US7435577B2 (en) | 2008-10-14 |
| CA2554776A1 (en) | 2005-08-18 |
| AU2005209856B2 (en) | 2010-06-10 |
| CA2554776C (en) | 2012-12-04 |
| ZA200606561B (en) | 2008-01-08 |
| EP1725650A2 (en) | 2006-11-29 |
| CN104931712A (zh) | 2015-09-23 |
| AU2005209856A1 (en) | 2005-08-18 |
| EP1725650B1 (en) | 2014-11-26 |
| PL1725650T3 (pl) | 2015-07-31 |
| US20050170447A1 (en) | 2005-08-04 |
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