DK2634243T3 - Forbedret fremgangsmåde til dyrkning af celler - Google Patents

Forbedret fremgangsmåde til dyrkning af celler Download PDF

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DK2634243T3
DK2634243T3 DK13169693.2T DK13169693T DK2634243T3 DK 2634243 T3 DK2634243 T3 DK 2634243T3 DK 13169693 T DK13169693 T DK 13169693T DK 2634243 T3 DK2634243 T3 DK 2634243T3
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cells
cell culture
biological substance
cell
batch
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Gerben Meile Zijlstra
Robert Patrick Hof
Jacob Schilder
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Patheon Holdings I B V
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
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    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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Claims (10)

  1. FORBEDRET FREMGANGSMÅDE TIL DYRKNING AF CELLER Patentkrav
    1. Fremgangsmåde til dyrkning af eukaryote celler i en reaktor i suspension i et celledyrkningsmedium, hvori cellerne producerer et ønsket biologisk stof udvalgt fra proteiner og vacciner, som kan anvendes som et aktivt stof i et farmaceutisk præparat, hvori mindst en celledyrkningsmediekomponent tilføres cellekulturen, og hvori cellekulturen, der omfatter cellerne, det ønskede biologiske stof og celledyrkningsmediet, cirkuleres over et filter ved anvendelse af et tangentialflow, og hvori filteret har en porestørrelse kendetegnet ved et molekylvægt-cutoff på mindst 30 kDa og højst 100 kDa, der er egnet til separering af det ønskede biologiske stof fra stoffer med en lavere molekylvægt end det ønskede biologiske stof, hvori udgangsflowet af væske fra filteret i det væsentlige kun indeholder komponenter med en molekylvægt, der er lavere end det ønskede biologiske stofs, og hvori det ønskede biologiske stof tilbageholdes i eller føres tilbage til reaktoren.
  2. 2. Fremgangsmåde ifølge krav 1, hvori det ønskede biologiske stof kodes for af mindst ét gen, der er transficeret ind i cellerne.
  3. 3. Fremgangsmåde ifølge et hvilket som helst af kravene 1-2, hvori det ønskede biologiske stof er et IgG.
  4. 4. Fremgangsmåde ifølge et hvilket som helst af kravene 1-3, hvori de eukaryote celler er pattedyrsceller.
  5. 5. Fremgangsmåde ifølge krav 4, hvori pattedyrscelleme er CHO-celler, hybridomer, BHK-celler, myelomceller, humane celler eller museceller.
  6. 6. Fremgangsmåde ifølge krav 5, hvori de humane celler er HEK-293-celler, humane lymfoblastoide celler eller El-immortaliserede HER-celler
  7. 7. Fremgangsmåde ifølge krav 5, hvori musecellerne er NSO-celler.
  8. 8. Fremgangsmåde ifølge krav 5, hvori pattedyrscelleme er CHO-celler.
  9. 9. Fremgangsmåde ifølge et hvilket som helst af kravene 1-8, hvori cellerne har en cellelevedygtighed på mindst 98 %.
  10. 10. Fremgangsmåde ifølge et hvilket som helst af kravene 1-9, hvori det ønskede biologiske stof høstes fra cellerne og/eller fra cellekulturen.
DK13169693.2T 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler DK2634243T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06014671 2006-07-14
EP07002571 2007-02-06
EP07765049.7A EP2041259B1 (en) 2006-07-14 2007-07-04 Improved process for the culturing of cells

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DK2634243T3 true DK2634243T3 (da) 2017-12-11

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Application Number Title Priority Date Filing Date
DK13169693.2T DK2634243T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler
DK13169685.8T DK2634242T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler
DK10183677.3T DK2343362T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler
DK07765049.7T DK2041259T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler

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DK13169685.8T DK2634242T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler
DK10183677.3T DK2343362T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler
DK07765049.7T DK2041259T3 (da) 2006-07-14 2007-07-04 Forbedret fremgangsmåde til dyrkning af celler

Country Status (21)

Country Link
US (7) US8119368B2 (da)
EP (5) EP2634243B1 (da)
JP (4) JP5092129B2 (da)
KR (3) KR101685246B1 (da)
CN (2) CN102876628A (da)
AR (1) AR062072A1 (da)
AU (1) AU2007272054C1 (da)
BR (1) BRPI0714347A2 (da)
CA (1) CA2657040C (da)
CL (1) CL2007002060A1 (da)
DK (4) DK2634243T3 (da)
EA (2) EA201101581A1 (da)
ES (5) ES2625062T3 (da)
HU (5) HUE032608T2 (da)
IL (2) IL196322A0 (da)
MX (2) MX2009000522A (da)
PL (5) PL2634243T3 (da)
PT (5) PT2634250T (da)
SI (5) SI2634250T1 (da)
TW (3) TWI512102B (da)
WO (1) WO2008006494A1 (da)

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