SG11202104417UA - Cell culture process by intensified perfusion with continuous harvest and without cell bleeding - Google Patents

Cell culture process by intensified perfusion with continuous harvest and without cell bleeding

Info

Publication number
SG11202104417UA
SG11202104417UA SG11202104417UA SG11202104417UA SG11202104417UA SG 11202104417U A SG11202104417U A SG 11202104417UA SG 11202104417U A SG11202104417U A SG 11202104417UA SG 11202104417U A SG11202104417U A SG 11202104417UA SG 11202104417U A SG11202104417U A SG 11202104417UA
Authority
SG
Singapore
Prior art keywords
cell
culture process
bleeding
cell culture
continuous harvest
Prior art date
Application number
SG11202104417UA
Inventor
Weichang Zhou
Hang Zhou
Mingyue Fang
Siyuan Tang
Original Assignee
Wuxi Biologics Ireland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuxi Biologics Ireland Ltd filed Critical Wuxi Biologics Ireland Ltd
Publication of SG11202104417UA publication Critical patent/SG11202104417UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
SG11202104417UA 2018-11-02 2019-09-29 Cell culture process by intensified perfusion with continuous harvest and without cell bleeding SG11202104417UA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2018113776 2018-11-02
CN2019089993 2019-06-04
PCT/CN2019/108921 WO2020088180A1 (en) 2018-11-02 2019-09-29 Cell culture process by intensified perfusion with continuous harvest and without cell bleeding

Publications (1)

Publication Number Publication Date
SG11202104417UA true SG11202104417UA (en) 2021-05-28

Family

ID=70463469

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202104417UA SG11202104417UA (en) 2018-11-02 2019-09-29 Cell culture process by intensified perfusion with continuous harvest and without cell bleeding

Country Status (10)

Country Link
US (1) US20220364034A1 (en)
EP (1) EP3874023A4 (en)
JP (2) JP2022506413A (en)
KR (1) KR102597919B1 (en)
CN (1) CN111406105A (en)
CA (1) CA3118398A1 (en)
MA (1) MA54093A (en)
SG (1) SG11202104417UA (en)
TW (1) TW202035681A (en)
WO (1) WO2020088180A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022007897A1 (en) * 2020-07-10 2022-01-13 青岛卓云海智医疗科技有限公司 Method for activating follicles by means of using small-molecule compound, and preparation thereof
JP2023546736A (en) * 2020-10-15 2023-11-07 エージーシー バイオロジクス，インコーポレーテッド Continuous high cell density culture using double-vessel tangential flow filtration
CN112592948B (en) * 2020-12-16 2023-05-09 广州汉腾生物科技有限公司 Perfusion culture method of animal cells
CN117561325A (en) * 2021-02-25 2024-02-13 安全电池股份有限公司 Fluid sampling system for biotechnology applications and methods of operation and use thereof
CN114230669B (en) * 2021-12-24 2024-01-30 天士力生物医药股份有限公司 Production method of bispecific antibody
WO2024006893A1 (en) * 2022-06-30 2024-01-04 Genentech, Inc. Systems, apparatus, and methods for cell culture
WO2024040519A1 (en) * 2022-08-25 2024-02-29 Wuxi Biologics Co., Ltd. Intermittent perfusion fed-batch culture

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2507552B2 (en) * 1988-08-29 1996-06-12 株式会社ニッショー Method for removing cell culture product and changing medium
PT2634250T (en) * 2006-07-14 2017-07-13 Patheon Holdings I B V Improved process for the culturing of cells
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US20090042253A1 (en) * 2007-08-09 2009-02-12 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
DE102008064279A1 (en) * 2008-12-20 2010-06-24 Bayer Technology Services Gmbh bioreactor
CN103305417A (en) * 2012-03-07 2013-09-18 无锡药明康德生物技术有限公司 High-yield reactor for protein production, and production method and application thereof
KR20220031937A (en) * 2013-09-16 2022-03-14 젠자임 코포레이션 Methods and systems for processing a cell culture
WO2015075070A1 (en) * 2013-11-20 2015-05-28 Cmc Biologics A/S A bioreactor system and method for producing a biopolymer
US20160312168A1 (en) * 2013-12-30 2016-10-27 Ge Healthcare Bio-Sciences Corp. Apparatus for cell cultivation
US10106829B2 (en) * 2014-01-29 2018-10-23 Amgen Inc. Overexpression of N-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
DK3152317T3 (en) * 2014-06-04 2019-04-08 Amgen Inc Methods for Harvesting Mammalian Cell Cultures
TWI797060B (en) * 2015-08-04 2023-04-01 美商再生元醫藥公司 Taurine supplemented cell culture medium and methods of use
JP7039477B2 (en) * 2015-11-10 2022-03-22 レプリゲン・コーポレイション Disposable filtration unit with alternating tangential flow
MX2019007564A (en) * 2016-12-23 2019-09-06 Serum Institute Of India Pvt Ltd Improved methods for enhancing antibody productivity in mammalian cell culture and minimizing aggregation during downstream, formulation processes and stable antibody formulations obtained thereof.
CN106987559A (en) * 2017-03-22 2017-07-28 上海药明生物技术有限公司 A kind of construction method of recombinant C HOK1 cell lines and its application
SG11201908891YA (en) * 2017-03-31 2019-10-30 Boehringer Ingelheim Int Perfusion medium
EP3601586A1 (en) * 2017-03-31 2020-02-05 Boehringer Ingelheim International GmbH Perfusion medium
CN108504562A (en) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 A kind of system of production of L-threonine by fermentation and its application

Also Published As

Publication number Publication date
CN111406105A (en) 2020-07-10
KR102597919B1 (en) 2023-11-06
US20220364034A1 (en) 2022-11-17
WO2020088180A1 (en) 2020-05-07
EP3874023A1 (en) 2021-09-08
MA54093A (en) 2021-09-08
TW202035681A (en) 2020-10-01
KR20210086655A (en) 2021-07-08
CA3118398A1 (en) 2020-05-07
JP2023109835A (en) 2023-08-08
EP3874023A4 (en) 2022-08-17
JP2022506413A (en) 2022-01-17

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