DK3152317T3 - Fremgangsmåder til at høste pattedyrscellekulturer - Google Patents

Fremgangsmåder til at høste pattedyrscellekulturer Download PDF

Info

Publication number
DK3152317T3
DK3152317T3 DK15730015.3T DK15730015T DK3152317T3 DK 3152317 T3 DK3152317 T3 DK 3152317T3 DK 15730015 T DK15730015 T DK 15730015T DK 3152317 T3 DK3152317 T3 DK 3152317T3
Authority
DK
Denmark
Prior art keywords
cell culture
cells
bioreactor
cell
recombinant protein
Prior art date
Application number
DK15730015.3T
Other languages
English (en)
Inventor
Chetan Goudar
Sean Cole
Henry Lin
Jonathan Lull
Tharmala Tharmalingam
Nicole Sabo
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53433304&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3152317(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of DK3152317T3 publication Critical patent/DK3152317T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Computer Hardware Design (AREA)
  • Water Supply & Treatment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Claims (15)

1. Fremgangsmåde til at høste et rekombinant protein, omfattende: i. at etablere en cellekultur ved at inokulere en bioreaktor med pattedyrsceller, som udtrykker et rekombinant protein, ii. at vedligeholde cellekulturen ved perfusion af cellekulturen med friske cellekulturmedium, som er formuleret eller suppleret for at opnå en koncentration på mindst 5 g/L af en ikke-ionisk blokcopolymer, iii. at lade cellekulturen passere gennem et filter af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der ikke tilbageholder det rekombinante protein i bioreaktoren, og iv. at opsamle et permeat, som indeholder det rekombinante protein.
2. Fremgangsmåde til at høste et rekombinant protein omfattende i. at etablere en cellekultur ved at inokulere en bioreaktor med pattedyrsceller, som udtrykker et rekombinant protein, ii. at vedligeholde cellekulturen ved perfusion af cellekulturen med friske cellekulturmedier, som er formuleret eller suppleret for at opnå en koncentration på mindst 1 g/L af en ikke-ionisk blokcopolymer og at lade cellekulturen passere gennem et filter af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der tilbageholder det rekombinante protein i bioreaktoren, og at opsamle et permeat, iii. når et forudbestemt parameter er nået, perfusion af cellekulturen med frisk cellekulturmedium, som er formuleret eller suppleret for at opnå en koncentration på mindst 5 g/L af en ikke-ionisk blokcopolymer og at lade cellekulturen passere gennem et filter af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der ikke tilbageholder det rekombinante protein i bioreaktoren og iv. at opsamle et permeat, som indeholde det rekombinante protein.
3. Fremgangsmåde ifølge krav 2, hvor filteret af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der tilbageholder det rekombinante protein i bioreaktoren og filteret af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der ikke tilbageholder det rekombinante protein i bioreaktoren er komponenter af et enkeltenhedsfiltersystem.
4. Fremgangsmåde ifølge kravene 1 -3, hvor filteret af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der ikke tilbageholder det rekombinante protein i bioreaktoren er et mikrofilter.
5. Fremgangsmåde ifølge krav 4, hvor molekylevægtgrænsen for mikrofilteret er mindst 500 kDa.
6. Fremgangsmåde ifølge krav 4, hvor molekylevægtgrænsen for mikrofilteret er 750 kDa.
7. Fremgangsmåde ifølge krav 4, hvor porestørrelsen på mikrofilteret er 0,1 mikrometer til 10 mikrometer.
8. Fremgangsmåde ifølge kravene 2-3, hvor filteret af hule fibre, som har en porestørrelse eller molekylevægtgrænse, der tilbageholder det rekombinante protein i bioreaktoren er et ultrafilter.
9. Fremgangsmåde ifølge krav 8, hvor molekylevægtgrænsen for ultrafilteret er 300 kDa eller mindre.
10. Fremgangsmåde ifølge krav 8, hvor molekylevægtgrænsen for ultrafilteret er 30 kDa.
11. Fremgangsmåde ifølge krav 8, hvor porestørrelsen på ultrafilteret er 0,01 mikrometer til 0,1 mikrometer.
12. Fremgangsmåde ifølge krav 3, hvor enkeltenhedsfiltersystemet er indeholdt i et hus.
13. Fremgangsmåde ifølge krav 3, hvor enkeltenhedsfiltersystemet yderligere omfatter et afstandsstykke mellem mindst to af filterkomponenterne af hule fibre.
14. Fremgangsmåde ifølge kravene 1-3, hvor den ikke-ioniske blokcopolymer er en polyoxypropylen-polyoxyethylen blokcopolymer.
15. Fremgangsmåde ifølge kravene 1-3, hvor den ikke-ioniske blokcopolymer er poloxamer 188.
DK15730015.3T 2014-06-04 2015-06-04 Fremgangsmåder til at høste pattedyrscellekulturer DK3152317T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462007588P 2014-06-04 2014-06-04
PCT/US2015/034297 WO2015188009A1 (en) 2014-06-04 2015-06-04 Methods for harvesting mammalian cell cultures

Publications (1)

Publication Number Publication Date
DK3152317T3 true DK3152317T3 (da) 2019-04-08

Family

ID=53433304

Family Applications (2)

Application Number Title Priority Date Filing Date
DK15730015.3T DK3152317T3 (da) 2014-06-04 2015-06-04 Fremgangsmåder til at høste pattedyrscellekulturer
DK21179944.0T DK3926051T3 (da) 2014-06-04 2015-06-04 Fremgangsmåder til høst af pattedyrecellekulturer

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK21179944.0T DK3926051T3 (da) 2014-06-04 2015-06-04 Fremgangsmåder til høst af pattedyrecellekulturer

Country Status (25)

Country Link
US (3) US11384378B2 (da)
EP (4) EP3926051B1 (da)
JP (3) JP6695814B2 (da)
KR (2) KR102598799B1 (da)
CN (1) CN106687575B (da)
AU (5) AU2015269365A1 (da)
BR (2) BR112016028285B1 (da)
CA (2) CA3195249A1 (da)
CL (2) CL2016003096A1 (da)
CY (1) CY1121217T1 (da)
DK (2) DK3152317T3 (da)
EA (2) EA039148B1 (da)
ES (3) ES2980493T3 (da)
FI (1) FI3926051T3 (da)
HU (2) HUE042277T2 (da)
IL (2) IL249150B (da)
LT (2) LT3152317T (da)
MX (3) MX2016015944A (da)
PL (2) PL3926051T3 (da)
PT (2) PT3152317T (da)
SG (2) SG10202113019XA (da)
SI (2) SI3926051T1 (da)
TR (1) TR201900776T4 (da)
WO (1) WO2015188009A1 (da)
ZA (1) ZA201608303B (da)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX369522B (es) 2013-02-22 2019-11-11 Genzyme Corp Metodos de cultivo por perfusión con microportadores y usos de los mismos.
SG11201506343QA (en) 2013-02-22 2015-09-29 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
SG11201705103WA (en) * 2014-12-22 2017-07-28 Genzyme Corp Methods of culturing a mammalian cell
JOP20160086B1 (ar) 2015-05-08 2021-08-17 2 Katholieke Univ Leuven Ku Leuven Research And Development مشتقات اندول مستبدلة احاديا او ثنائيا بصفتها مانعات للتكاثر الفيروسي لحمى الفنك
CN114058599A (zh) * 2016-07-25 2022-02-18 瑞普利金公司 交替切向流快速收获
JP7043125B2 (ja) * 2016-10-28 2022-03-29 旭化成メディカル株式会社 連続培養における有用物質の回収方法
CN107964037A (zh) * 2017-05-16 2018-04-27 浙江海隆生物科技有限公司 基于cho细胞表达系统的重组猪瘟e2蛋白的纯化方法及应用
EP3675988A2 (en) * 2017-08-31 2020-07-08 Massachusetts Institute of Technology Hollow fibre membrane filtration systems and methods for manufacturing biologically-produced products
IL273549B2 (en) * 2017-10-06 2023-10-01 Lonza Ag Automated control of cell culture using Raman spectroscopy
EP3700505A4 (en) * 2017-10-26 2021-01-06 Repligen Corporation MICRO-ALTERNATIVE TANGENTIAL FLOW INFUSION FILTER, PROCESSING CONTAINER, AND METHODS OF USE THEREOF
KR102589415B1 (ko) * 2017-11-20 2023-10-16 론자 리미티드 락테이트 축적을 방지하면서 세포 배양물을 증식하는 방법 및 시스템
CN111868249B (zh) * 2018-03-19 2024-06-18 富士胶片株式会社 生产物的制造方法
GB201810772D0 (en) * 2018-06-29 2018-08-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
KR102597919B1 (ko) * 2018-11-02 2023-11-06 우시 바이올로직스 아일랜드 리미티드 지속적으로 수확하고 세포 출혈없는 강화된 관류에 의한 세포 배양 과정
CA3121804A1 (en) * 2018-12-21 2020-06-25 Genentech, Inc. Methods of producing polypeptides using a cell line resistant to apoptosis
WO2020142445A1 (en) 2018-12-31 2020-07-09 Repligen Corporation Filter for mammalian cell culture perfusion and clarification with hydrophobic hollow fiber
EP4013849A4 (en) * 2019-08-13 2022-10-26 Repligen Corporation CONTROL SYSTEMS AND METHODS FOR AUTOMATED HIGH SOLIDS CELL CULTURE CLARIFICATION
KR20220123463A (ko) * 2020-01-10 2022-09-06 론자 워커스빌 아이엔씨. 생물학적 공정 및 세포 집단을 정제하기 위한 필터 장치 및 방법
WO2021143699A1 (en) * 2020-01-15 2021-07-22 Wuxi Biologics (Shanghai) Co. Ltd. An apparatus and a method for continuously harvesting a biological substance produced by a cultured cell
CN111808797A (zh) * 2020-07-28 2020-10-23 湖南赛诺生物科技股份有限公司 Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂
US20240043896A1 (en) * 2020-10-15 2024-02-08 Agc Biologics, Inc. Continuous high cell-density culture with dual-vessel tangential flow filtration
US20220119759A1 (en) * 2020-10-19 2022-04-21 Emd Millipore Corporation Cascade Tangential Flow Filtration Systems for Perfusion of Cell Culture
US20220268785A1 (en) * 2021-02-19 2022-08-25 Northwestern University Facile sample preparation for quantitative single-cell proteomics
JP2024507578A (ja) * 2021-02-25 2024-02-20 セキュアセル アクチェンゲゼルシャフト バイオテクノロジー用途のための流体サンプリングシステム、その操作方法および使用
FR3126713A1 (fr) * 2021-09-08 2023-03-10 Erypharm Procédé de production de cellules de culture à haute densité
CN114317392A (zh) * 2021-12-27 2022-04-12 无锡药明生物技术股份有限公司 优化细胞培养的方法
WO2023167584A1 (en) * 2022-03-01 2023-09-07 Biosanapharma B.V. Continuous manufacturing of a biological substance with process traceability
WO2023200918A1 (en) * 2022-04-14 2023-10-19 Terasaki Institute For Biomedical Innovation Systems and methods for recycling growth factors and other components
WO2023205075A1 (en) * 2022-04-22 2023-10-26 Coopersurgical, Inc. Methods of culturing umbilical cord mesenchymal stem cells
WO2024059235A2 (en) * 2022-09-16 2024-03-21 Amgen Inc. A method for harvesting products from perfusion cultures
WO2024173685A1 (en) * 2023-02-15 2024-08-22 The Board Of Trustees Of The Leland Stanford Junior University Metabolic selection for glycogen-storing cells in vitro

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936694B1 (en) 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
US5223428A (en) 1982-12-14 1993-06-29 Baxter International Inc. Method for in vitro culture of mammalian cells
AU588819B2 (en) 1984-10-29 1989-09-28 Immunex Corporation Cloning of human granulocyte-macrophage colony stimulating factor gene
US4968607A (en) 1987-11-25 1990-11-06 Immunex Corporation Interleukin-1 receptors
US5075222A (en) 1988-05-27 1991-12-24 Synergen, Inc. Interleukin-1 inhibitors
JP2507552B2 (ja) * 1988-08-29 1996-06-12 株式会社ニッショー 細胞培養産生物の取出しおよび培地の交換方法
WO1990005183A1 (en) 1988-10-31 1990-05-17 Immunex Corporation Interleukin-4 receptors
US4937196A (en) 1989-08-18 1990-06-26 Brunswick Corporation Membrane bioreactor system
US5395760A (en) 1989-09-05 1995-03-07 Immunex Corporation DNA encoding tumor necrosis factor-α and -β receptors
DK0417563T3 (da) 1989-09-12 2000-11-06 Hoffmann La Roche TNF-bindende proteiner
US6204363B1 (en) 1989-10-16 2001-03-20 Amgen Inc. Stem cell factor
US5272064A (en) 1989-12-19 1993-12-21 Amgen Inc. DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof
US5149792A (en) 1989-12-19 1992-09-22 Amgen Inc. Platelet-derived growth factor B chain analogs
US5378612A (en) 1990-05-11 1995-01-03 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Culture medium for production of recombinant protein
WO1991018982A1 (en) 1990-06-05 1991-12-12 Immunex Corporation Type ii interleukin-1 receptors
US5350683A (en) 1990-06-05 1994-09-27 Immunex Corporation DNA encoding type II interleukin-1 receptors
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
EP0672141B1 (en) 1992-10-23 2003-05-14 Immunex Corporation Methods of preparing soluble, oligomeric proteins
US5554512A (en) 1993-05-24 1996-09-10 Immunex Corporation Ligands for flt3 receptors
US5981713A (en) 1994-10-13 1999-11-09 Applied Research Systems Ars Holding N.V. Antibodies to intereleukin-1 antagonists
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
EP1666591B1 (en) 1995-06-29 2011-03-23 Immunex Corporation Cytokine that induces apoptosis
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US6096728A (en) 1996-02-09 2000-08-01 Amgen Inc. Composition and method for treating inflammatory diseases
US6271349B1 (en) 1996-12-23 2001-08-07 Immunex Corporation Receptor activator of NF-κB
US6235883B1 (en) 1997-05-05 2001-05-22 Abgenix, Inc. Human monoclonal antibodies to epidermal growth factor receptor
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US6337072B1 (en) 1998-04-03 2002-01-08 Hyseq, Inc. Interleukin-1 receptor antagonist and recombinant production thereof
US6068775A (en) * 1998-04-13 2000-05-30 Circe Biomedical, Inc. Removal of agent from cell suspension
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
WO2001036637A1 (en) 1999-11-17 2001-05-25 Immunex Corporation Receptor activator of nf-kappa b
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
AR031699A1 (es) 2000-05-26 2003-10-01 Bristol Myers Squibb Co Molecula mutante de ctla4 soluble que se enlaza a cd80 y/o cd86, molecula de acido nucleico que la codifica, vector y sistema vector que comprende a esta ultima, celula huesped que lo posee, metodo para producir una proteina mutante y dicha proteina, uso de la molecula mutante para preparar un medic
US20040185535A1 (en) 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
WO2002029025A2 (en) 2000-10-02 2002-04-11 Novo Nordisk A/S Factor vii glycoforms
MY143582A (en) * 2001-06-26 2011-05-31 Amgent Fremont Inc Antibodies to opgl
CA2491992A1 (en) 2002-07-09 2004-01-15 Baxter International, Inc. Animal protein free media for cultivation of cells
NZ593428A (en) * 2002-09-06 2013-01-25 Amgen Inc Therapeutic human anti-il-1r1 monoclonal antibody
JP4541157B2 (ja) * 2002-12-23 2010-09-08 ブリストル−マイヤーズ スクイブ カンパニー タンパク質を製造するための哺乳類細胞培養法
DE10312765A1 (de) 2003-03-21 2004-09-30 Bayer Technology Services Gmbh Vorrichtung und Verfahren zur Sterilisation flüssiger Medien mittels UV-Bestrahlung und Kurzzeiterhitzung
US20050100965A1 (en) * 2003-11-12 2005-05-12 Tariq Ghayur IL-18 binding proteins
US7795010B2 (en) * 2004-01-21 2010-09-14 Toray Industries, Inc. Fractionator and method of fractionation
JP2007525984A (ja) * 2004-03-05 2007-09-13 ディーエスエム アイピー アセッツ ビー．ブイ． 連続的灌流および交互接線流による細胞培養の方法
US7422875B2 (en) 2004-07-20 2008-09-09 Board Of Regents Of The University Of Nebraska Compositions and methods for increasing protein production
US8053238B2 (en) 2005-10-31 2011-11-08 Unhwa Corporation Isolated population of plant single cells and method of preparing the same
HUE034299T2 (en) 2006-07-14 2018-02-28 Patheon Holdings I B V An improved method for cell culture
WO2008024769A2 (en) 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Intensified perfusion production method
MY185887A (en) 2006-09-13 2021-06-14 Abbvie Inc Cell culture improvements
US20100221823A1 (en) 2007-06-11 2010-09-02 Amgen Inc. Method for culturing mammalian cells to improve recombinant protein production
EP2014760A1 (en) * 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
JP5579599B2 (ja) 2007-06-15 2014-08-27 アムジエン・インコーポレーテツド バイオリアクターにおいて使用する細胞培養培地の処理方法
EP3327132A3 (en) * 2007-08-09 2018-07-18 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
CN106222220A (zh) 2007-12-27 2016-12-14 百深有限责任公司 细胞培养方法
DE102008051574A1 (de) 2008-10-14 2010-04-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Herstellung von Interferon-beta und deren Varianten
EP2977461A1 (en) * 2008-11-12 2016-01-27 Baxalta Incorporated Method of producing serum-free insulin-free factor vii
EA023193B1 (ru) 2009-07-31 2016-05-31 Бакстер Интернэшнл Инк. Среда для культивирования клеток для экспрессии белков adamts
KR102223417B1 (ko) * 2009-10-26 2021-03-05 에프. 호프만-라 로슈 아게 글리코실화된 면역글로불린의 제조 방법
MX2012007117A (es) 2009-12-23 2012-07-23 Sanofi Pasteur Metodo para cultivar celulas adherentes.
CA2807768A1 (en) 2010-08-25 2012-03-01 Jerry Shevitz Enclosed filtration system with three chambers
JP5893051B2 (ja) 2011-02-10 2016-03-23 クルセル ホランド ベー ヴェー 交互空気圧膜式細胞分離システム
BR112013021351A2 (pt) 2011-02-23 2016-09-20 Amgen Inc meio de cultura de célula para exposição à luz ultravioleta e métodos relacionados ao mesmo
US9133493B2 (en) 2011-04-21 2015-09-15 Amgen Inc. Method for culturing mammalian cells to improve recombinant protein production
EP4353743A3 (en) 2011-05-13 2024-07-17 Octapharma AG A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii
SG10201702537RA (en) 2011-07-01 2017-04-27 Amgen Inc Mammalian cell culture
US9803166B2 (en) 2011-09-16 2017-10-31 Amgen Inc. Pre-programmed non-feedback controlled continuous feeding of cell cultures
AU2012328753A1 (en) 2011-10-26 2014-04-24 Amgen Inc. Methods of reducing or eliminating protein modification and degradation arising from exposure to UV light
WO2013138159A1 (en) 2012-03-15 2013-09-19 Amgen Inc. Methods of determining exposure to uv light
US10481164B2 (en) 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
WO2014022102A1 (en) 2012-08-01 2014-02-06 Amgen Inc. Methods of using anti-apoptotic compounds to modulate one or more properties of a cell culture
TWI637057B (zh) 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
PL2735326T3 (pl) 2012-11-26 2017-08-31 Gambro Lundia Ab Układ wspomagania wątroby
US10316282B2 (en) * 2013-03-19 2019-06-11 Cmc Biologics A/S Method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process

Also Published As

Publication number Publication date
US11427848B2 (en) 2022-08-30
KR102391259B1 (ko) 2022-04-26
JP2017519497A (ja) 2017-07-20
MX2021008143A (es) 2021-08-11
AU2019203538B2 (en) 2020-10-08
EP4372078A3 (en) 2024-09-04
IL249150B (en) 2022-09-01
CN106687575B (zh) 2021-04-30
PT3152317T (pt) 2019-04-18
IL249150A0 (en) 2017-01-31
JP2022126811A (ja) 2022-08-30
JP7097404B2 (ja) 2022-07-07
BR112016028285A2 (pt) 2018-06-26
AU2015269365A1 (en) 2016-12-15
BR122018011329A2 (pt) 2019-09-10
US20170114381A1 (en) 2017-04-27
ZA201608303B (en) 2023-06-28
HUE042277T2 (hu) 2019-06-28
WO2015188009A1 (en) 2015-12-10
JP7377316B2 (ja) 2023-11-09
EP3521440B1 (en) 2022-02-09
CL2019001641A1 (es) 2019-10-25
CA3195249A1 (en) 2015-12-10
KR102598799B1 (ko) 2023-11-07
LT3152317T (lt) 2019-03-12
KR20170015464A (ko) 2017-02-08
SI3152317T1 (sl) 2019-03-29
EA202192126A2 (ru) 2021-11-30
EP3926051A1 (en) 2021-12-22
EP3521440A1 (en) 2019-08-07
JP2020124207A (ja) 2020-08-20
EA201692498A1 (ru) 2017-05-31
AU2022224699B2 (en) 2024-08-29
US20220356502A1 (en) 2022-11-10
SI3926051T1 (sl) 2024-06-28
AU2020294155A1 (en) 2021-01-28
TR201900776T4 (tr) 2019-02-21
PT3926051T (pt) 2024-06-20
AU2020294155B2 (en) 2022-07-28
CA2951046A1 (en) 2015-12-10
EA039148B1 (ru) 2021-12-10
CY1121217T1 (el) 2020-05-29
EP3152317A1 (en) 2017-04-12
EA202192126A3 (ru) 2022-03-31
AU2024267090A1 (en) 2024-12-19
KR20220054903A (ko) 2022-05-03
ES2909630T3 (es) 2022-05-09
BR112016028285B1 (pt) 2023-02-23
US11384378B2 (en) 2022-07-12
MX2016015944A (es) 2017-08-15
ES2709994T3 (es) 2019-04-22
EP4372078A2 (en) 2024-05-22
SG11201610143VA (en) 2017-01-27
CA2951046C (en) 2023-06-13
ES2980493T3 (es) 2024-10-01
US20220136025A1 (en) 2022-05-05
AU2019203538A1 (en) 2019-06-06
PL3152317T3 (pl) 2019-08-30
MX2021008142A (es) 2021-08-11
AU2022224699A1 (en) 2022-09-22
PL3926051T3 (pl) 2024-08-26
HUE066803T2 (hu) 2024-09-28
JP6695814B2 (ja) 2020-05-20
CL2016003096A1 (es) 2017-11-03
DK3926051T3 (da) 2024-06-10
SG10202113019XA (en) 2021-12-30
EP3152317B1 (en) 2019-01-02
EP3926051B1 (en) 2024-05-08
IL294430A (en) 2022-09-01
FI3926051T3 (fi) 2024-06-18
LT3926051T (lt) 2024-06-10
CN106687575A (zh) 2017-05-17
WO2015188009A9 (en) 2017-01-26

Similar Documents

Publication Publication Date Title
US11427848B2 (en) Methods for harvesting mammalian cell cultures
AU2024204313A1 (en) Process For Manipulating The Level Of Glycan Content Of A Glycoprotein