DK2530149T3 - Nukleinsyrestruktur indeholdende en pyripyropenbiosyntesegenklynge og et markørgen - Google Patents

Nukleinsyrestruktur indeholdende en pyripyropenbiosyntesegenklynge og et markørgen Download PDF

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DK2530149T3
DK2530149T3 DK11736900.9T DK11736900T DK2530149T3 DK 2530149 T3 DK2530149 T3 DK 2530149T3 DK 11736900 T DK11736900 T DK 11736900T DK 2530149 T3 DK2530149 T3 DK 2530149T3
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Hiroyuki Anzai
Sato Aihara
Naomi Sumida
Koichiro Murashima
Kouji Yanai
Kentaro Yamamoto
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Meiji Seika Pharma Co Ltd
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
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  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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  • Saccharide Compounds (AREA)

Claims (10)

1. Nukleinsyrekonstrukt omfattende en pyripyropenbiosyntesegenklynge og et markørgen, hvor pyripyropenbiosyntesegenklyngen består af den fulde længde af mindst én nukleotidsekvens udvalgt blandt nukleotidsekvenserne (I) og (II) nedenfor: (I) en nukleotidsekvens fra 2911 til 27797 i SEQ ID NO:266; og (II) en nukleotidsekvens fra 1 til 25000 eller fra 2446 til 27505 i SEQ ID NO:266.
2. Nukleinsyrekonstrukt ifølge krav 1, hvor pyripyropenbiosyntesegenklyngen omfatter et gen af interesse og en ekspressionsstyrende region.
3. Nukleinsyrekonstrukt ifølge krav 1, hvor markørgenet er et lægemiddelresistensgen eller et auxotrofigen.
4. Nukleinsyrekonstrukt ifølge krav 1, hvor markørgenet er et destomycinresistensgen eller hygromycinresistensgen.
5. Mikroorganismetransformant, som er en trådsvamp, der danner pyripyropener, hvor nukleinsyrekonstruktet ifølge krav 1 er blevet indbragt.
6. Mikroorganismetransformant ifølge krav 5, hvori et nukleinsyrekonstrukt omfattende en pyripyropenbiosyntesegenklynge og nukleinsyrekonstruktet omfattende et markørgen er blevet indbragt samtidigt eller særskilt.
7. Mikroorganismetransformant ifølge krav 5, hvor trådsvampen, der danner pyripyropener, tilhører slægten Penicillium. slægten Eupenicillium eller slægten Aspergillus.
8. Mikroorganismetransformant ifølge krav 5, hvor trådsvampen, der danner pyripyropener, er Penicillium coprobium, Penicillium griseofulvum. Eupenicillium reticulosporum eller Aspergillus fumigatus.
9. Mikroorganismetransformant ifølge krav 5, hvor trådsvampen, der danner pyripyropener, er Penicillium coprobium stamme PF 1169 eller Penicillium coprobium stamme ATCC58615.
10. Fremgangsmåde til dannelse af pyripyropener, hvilken fremgangsmåde omfatter at dyrke mikroorganismetransformanten ifølge et hvilket som helst af kravene 5 til 9 og at indvinde pyripyropenerne fra en kultur.
DK11736900.9T 2010-01-26 2011-01-19 Nukleinsyrestruktur indeholdende en pyripyropenbiosyntesegenklynge og et markørgen DK2530149T3 (da)

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JP2010014700 2010-01-26
JP2010253183 2010-11-11
PCT/JP2011/050852 WO2011093186A1 (ja) 2010-01-26 2011-01-19 ピリピロペン生合成遺伝子群およびマーカー遺伝子を含む核酸構築物

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DK2530149T3 true DK2530149T3 (da) 2017-12-04

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US (1) US9090924B2 (da)
EP (1) EP2530149B1 (da)
JP (1) JP5864267B2 (da)
KR (2) KR101831121B1 (da)
CN (1) CN102884187B (da)
AU (1) AU2011210968B2 (da)
BR (1) BR112012019458B8 (da)
CA (1) CA2788080C (da)
DK (1) DK2530149T3 (da)
ES (1) ES2655615T3 (da)
IL (1) IL221129B (da)
MX (1) MX340373B (da)
NZ (1) NZ601951A (da)
PL (1) PL2530149T3 (da)
RU (1) RU2576001C2 (da)
TW (1) TWI633185B (da)
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Publication number Priority date Publication date Assignee Title
AU2011210967B2 (en) * 2010-01-26 2016-06-23 Mitsui Chemicals Crop & Life Solutions, Inc. Method for manufacturing a pyripyropene
WO2013073689A1 (ja) * 2011-11-17 2013-05-23 Ｍｅｉｊｉ Ｓｅｉｋａファルマ株式会社 ピリピロペン生合成遺伝子発現植物体
CN116904328B (zh) * 2023-07-13 2024-08-02 山东大学 一种高表达啶南平a的工程菌及发酵培养基

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS631528A (ja) 1986-06-20 1988-01-06 Polyurethan Kasei Kk ガラス繊維入りポリウレタンフオ−ムの製造方法
JP2993767B2 (ja) 1991-06-06 1999-12-27 社団法人北里研究所 Ｆｏ−１２８９ａ物質およびその製造法
ES2131578T5 (es) * 1992-04-30 2010-03-31 Institut Pasteur Deteccion rapida de resistencia a antibioticos en mycobacterium tuber culosis.
JP3233476B2 (ja) 1992-10-22 2001-11-26 社団法人北里研究所 Ｆｏ−１２８９物質およびその製造法
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
ATE256119T1 (de) * 1994-05-26 2003-12-15 Canon Kk Verfahren zum nachweis von einer zielsubstanz in einer probe mit hilfe von pyryliumverbindung
JP4114958B2 (ja) * 1994-06-07 2008-07-09 キヤノン株式会社 核酸ハイブリッド体の検出方法及びそれに用いるプローブ
JPH08239385A (ja) 1995-03-03 1996-09-17 Kitasato Inst:The Ｆｏ−１２８９物質およびその製造法
JP3725198B2 (ja) 1995-03-27 2005-12-07 社団法人北里研究所 ピリピロペン誘導体
JPH08269063A (ja) 1995-03-28 1996-10-15 Kitasato Inst:The ピリピロペン誘導体
JPH08269062A (ja) 1995-03-30 1996-10-15 Kitasato Inst:The ピリピロペン誘導体
JPH08269064A (ja) 1995-04-03 1996-10-15 Kitasato Inst:The ピリピロペン誘導体
JPH08269065A (ja) 1995-04-03 1996-10-15 Kitasato Inst:The ピリピロペン誘導体
JPH08269066A (ja) 1995-04-03 1996-10-15 Kitasato Inst:The ピリピロペン誘導体
JP3688337B2 (ja) 1995-04-18 2005-08-24 社団法人北里研究所 ピリピロペン誘導体
ATE371741T1 (de) 1996-07-24 2007-09-15 Meiji Seika Kaisha Systeme zur massenherstellung von proteinen oder peptiden durch mikroorganismen der gattung humicola
US7285404B1 (en) 1999-09-07 2007-10-23 Meiji Seika Kaisha, Ltd. Cyclic depsipeptide synthetase and method for recombinant production
KR100522446B1 (ko) 2003-01-07 2005-10-18 한국생명공학연구원 아실 코에이:콜레스테롤 아실 트란스퍼라제의 저해활성을갖는 화합물 또는 그 염을 유효성분으로 하는 살충제
US20060135645A1 (en) 2004-12-17 2006-06-22 David Glassel Pest protection methods and compositions
WO2006083411A2 (en) 2004-12-17 2006-08-10 David Glassel Methods and compositions of matter for treatment of cellulose
US7491738B2 (en) 2005-06-01 2009-02-17 Meiji Seika Kaisha, Ltd. Pest control agents
TWI388282B (zh) 2005-06-01 2013-03-11 Meiji Seika Pharma Co Ltd 害蟲控制劑
WO2008066153A1 (fr) 2006-11-30 2008-06-05 Meiji Seika Kaisha, Ltd. Agent antiparasitaire
EP2592084B8 (en) 2007-08-13 2014-08-27 Basf Se Process for producing pyripyropene derivatives and intermediates for the production thereof.
US8557550B2 (en) * 2008-07-24 2013-10-15 Meija Seika Pharma Co., Ltd. Pyripyropene a biosynthetic gene

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Publication number Publication date
MX340373B (es) 2016-07-07
AU2011210968B2 (en) 2015-05-07
EP2530149A1 (en) 2012-12-05
ES2655615T3 (es) 2018-02-20
NZ601951A (en) 2015-01-30
BR112012019458B1 (pt) 2021-02-17
EP2530149B1 (en) 2017-10-25
BR112012019458A2 (pt) 2015-09-15
BR112012019458B8 (pt) 2022-08-02
AU2011210968A1 (en) 2012-09-13
RU2576001C2 (ru) 2016-02-27
RU2012136448A (ru) 2014-03-10
CA2788080C (en) 2018-04-17
JP5864267B2 (ja) 2016-02-17
US20130017581A1 (en) 2013-01-17
CA2788080A1 (en) 2011-08-04
EP2530149A4 (en) 2014-01-15
MX2012008687A (es) 2012-08-23
WO2011093186A1 (ja) 2011-08-04
CN102884187A (zh) 2013-01-16
IL221129B (en) 2018-06-28
JPWO2011093186A1 (ja) 2013-06-06
TWI633185B (zh) 2018-08-21
KR20170125947A (ko) 2017-11-15
KR101831121B1 (ko) 2018-02-21
PL2530149T3 (pl) 2018-04-30
KR20120107519A (ko) 2012-10-02
TW201139669A (en) 2011-11-16
CN102884187B (zh) 2017-03-01
US9090924B2 (en) 2015-07-28

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