CN111217897B - Os516蛋白及其编码基因和应用 - Google Patents
Os516蛋白及其编码基因和应用 Download PDFInfo
- Publication number
- CN111217897B CN111217897B CN202010142560.6A CN202010142560A CN111217897B CN 111217897 B CN111217897 B CN 111217897B CN 202010142560 A CN202010142560 A CN 202010142560A CN 111217897 B CN111217897 B CN 111217897B
- Authority
- CN
- China
- Prior art keywords
- plant
- protein
- sequence
- gene
- glu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 95
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 58
- 241000196324 Embryophyta Species 0.000 claims abstract description 110
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 27
- 235000009566 rice Nutrition 0.000 claims abstract description 23
- 238000010362 genome editing Methods 0.000 claims abstract description 22
- 230000009261 transgenic effect Effects 0.000 claims abstract description 16
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 6
- 108020004414 DNA Proteins 0.000 claims description 32
- 102000053602 DNA Human genes 0.000 claims description 24
- 241000209094 Oryza Species 0.000 claims description 23
- 108091033409 CRISPR Proteins 0.000 claims description 17
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 108091026890 Coding region Proteins 0.000 claims description 4
- 108020001507 fusion proteins Proteins 0.000 claims description 4
- 102000037865 fusion proteins Human genes 0.000 claims description 4
- 230000001276 controlling effect Effects 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 230000005764 inhibitory process Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 15
- 238000003976 plant breeding Methods 0.000 abstract description 8
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 240000007594 Oryza sativa Species 0.000 abstract description 5
- 230000006872 improvement Effects 0.000 abstract description 3
- 108091027544 Subgenomic mRNA Proteins 0.000 description 20
- 239000013612 plasmid Substances 0.000 description 14
- 239000002773 nucleotide Substances 0.000 description 7
- 125000003729 nucleotide group Chemical group 0.000 description 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 239000013604 expression vector Substances 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 5
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 5
- 239000004009 herbicide Substances 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- 241000589158 Agrobacterium Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000002363 herbicidal effect Effects 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 108010049041 glutamylalanine Proteins 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- RMAWDDRDTRSZIR-ZLUOBGJFSA-N Ala-Ser-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O RMAWDDRDTRSZIR-ZLUOBGJFSA-N 0.000 description 2
- ZJIFRAPZHAGLGR-MELADBBJSA-N Asn-Phe-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CC=CC=C2)NC(=O)[C@H](CC(=O)N)N)C(=O)O ZJIFRAPZHAGLGR-MELADBBJSA-N 0.000 description 2
- IKFZXRLDMYWNBU-YUMQZZPRSA-N Gln-Gly-Arg Chemical compound NC(=O)CC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCN=C(N)N IKFZXRLDMYWNBU-YUMQZZPRSA-N 0.000 description 2
- YKLNMGJYMNPBCP-ACZMJKKPSA-N Glu-Asn-Asp Chemical compound C(CC(=O)O)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(=O)O)C(=O)O)N YKLNMGJYMNPBCP-ACZMJKKPSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 108010068380 arginylarginine Proteins 0.000 description 2
- 108010040443 aspartyl-aspartic acid Proteins 0.000 description 2
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 2
- 108010093581 aspartyl-proline Proteins 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 108010034529 leucyl-lysine Proteins 0.000 description 2
- 108010064235 lysylglycine Proteins 0.000 description 2
- 108010038320 lysylphenylalanine Proteins 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 108010069117 seryl-lysyl-aspartic acid Proteins 0.000 description 2
- 108010071207 serylmethionine Proteins 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- IMIZPWSVYADSCN-UHFFFAOYSA-N 4-methyl-2-[[4-methyl-2-[[4-methyl-2-(pyrrolidine-2-carbonylamino)pentanoyl]amino]pentanoyl]amino]pentanoic acid Chemical compound CC(C)CC(C(O)=O)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C1CCCN1 IMIZPWSVYADSCN-UHFFFAOYSA-N 0.000 description 1
- YLTKNGYYPIWKHZ-ACZMJKKPSA-N Ala-Ala-Glu Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCC(O)=O YLTKNGYYPIWKHZ-ACZMJKKPSA-N 0.000 description 1
- CXRCVCURMBFFOL-FXQIFTODSA-N Ala-Ala-Pro Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(O)=O CXRCVCURMBFFOL-FXQIFTODSA-N 0.000 description 1
- NHCPCLJZRSIDHS-ZLUOBGJFSA-N Ala-Asp-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(O)=O NHCPCLJZRSIDHS-ZLUOBGJFSA-N 0.000 description 1
- RXTBLQVXNIECFP-FXQIFTODSA-N Ala-Gln-Gln Chemical compound C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O RXTBLQVXNIECFP-FXQIFTODSA-N 0.000 description 1
- VGPWRRFOPXVGOH-BYPYZUCNSA-N Ala-Gly-Gly Chemical compound C[C@H](N)C(=O)NCC(=O)NCC(O)=O VGPWRRFOPXVGOH-BYPYZUCNSA-N 0.000 description 1
- BTBUEVAGZCKULD-XPUUQOCRSA-N Ala-Gly-His Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC1=CN=CN1 BTBUEVAGZCKULD-XPUUQOCRSA-N 0.000 description 1
- DVJSJDDYCYSMFR-ZKWXMUAHSA-N Ala-Ile-Gly Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O DVJSJDDYCYSMFR-ZKWXMUAHSA-N 0.000 description 1
- BLTRAARCJYVJKV-QEJZJMRPSA-N Ala-Lys-Phe Chemical compound C[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(O)=O BLTRAARCJYVJKV-QEJZJMRPSA-N 0.000 description 1
- MDNAVFBZPROEHO-UHFFFAOYSA-N Ala-Lys-Val Natural products CC(C)C(C(O)=O)NC(=O)C(NC(=O)C(C)N)CCCCN MDNAVFBZPROEHO-UHFFFAOYSA-N 0.000 description 1
- VHEVVUZDDUCAKU-FXQIFTODSA-N Ala-Met-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(O)=O VHEVVUZDDUCAKU-FXQIFTODSA-N 0.000 description 1
- CNQAFFMNJIQYGX-DRZSPHRISA-N Ala-Phe-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=CC=C1 CNQAFFMNJIQYGX-DRZSPHRISA-N 0.000 description 1
- VNFSAYFQLXPHPY-CIQUZCHMSA-N Ala-Thr-Ile Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VNFSAYFQLXPHPY-CIQUZCHMSA-N 0.000 description 1
- JJHBEVZAZXZREW-LFSVMHDDSA-N Ala-Thr-Phe Chemical compound C[C@@H](O)[C@H](NC(=O)[C@H](C)N)C(=O)N[C@@H](Cc1ccccc1)C(O)=O JJHBEVZAZXZREW-LFSVMHDDSA-N 0.000 description 1
- NLYYHIKRBRMAJV-AEJSXWLSSA-N Ala-Val-Pro Chemical compound C[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N NLYYHIKRBRMAJV-AEJSXWLSSA-N 0.000 description 1
- REWSWYIDQIELBE-FXQIFTODSA-N Ala-Val-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O REWSWYIDQIELBE-FXQIFTODSA-N 0.000 description 1
- XPSGESXVBSQZPL-SRVKXCTJSA-N Arg-Arg-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O XPSGESXVBSQZPL-SRVKXCTJSA-N 0.000 description 1
- VWVPYNGMOCSSGK-GUBZILKMSA-N Arg-Arg-Asn Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(O)=O VWVPYNGMOCSSGK-GUBZILKMSA-N 0.000 description 1
- BEXGZLUHRXTZCC-CIUDSAMLSA-N Arg-Gln-Ser Chemical compound C(C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CO)C(=O)O)N)CN=C(N)N BEXGZLUHRXTZCC-CIUDSAMLSA-N 0.000 description 1
- HQIZDMIGUJOSNI-IUCAKERBSA-N Arg-Gly-Arg Chemical compound N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O HQIZDMIGUJOSNI-IUCAKERBSA-N 0.000 description 1
- IRRMIGDCPOPZJW-ULQDDVLXSA-N Arg-His-Phe Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O IRRMIGDCPOPZJW-ULQDDVLXSA-N 0.000 description 1
- FRMQITGHXMUNDF-GMOBBJLQSA-N Arg-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N FRMQITGHXMUNDF-GMOBBJLQSA-N 0.000 description 1
- HCIUUZGFTDTEGM-NAKRPEOUSA-N Arg-Ile-Cys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N HCIUUZGFTDTEGM-NAKRPEOUSA-N 0.000 description 1
- OTZMRMHZCMZOJZ-SRVKXCTJSA-N Arg-Leu-Glu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O OTZMRMHZCMZOJZ-SRVKXCTJSA-N 0.000 description 1
- UZGFHWIJWPUPOH-IHRRRGAJSA-N Arg-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N UZGFHWIJWPUPOH-IHRRRGAJSA-N 0.000 description 1
- YVTHEZNOKSAWRW-DCAQKATOSA-N Arg-Lys-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O YVTHEZNOKSAWRW-DCAQKATOSA-N 0.000 description 1
- NPAVRDPEFVKELR-DCAQKATOSA-N Arg-Lys-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O NPAVRDPEFVKELR-DCAQKATOSA-N 0.000 description 1
- XKDYWGLNSCNRGW-WDSOQIARSA-N Arg-Lys-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CCCN=C(N)N)CCCCN)C(O)=O)=CNC2=C1 XKDYWGLNSCNRGW-WDSOQIARSA-N 0.000 description 1
- HGKHPCFTRQDHCU-IUCAKERBSA-N Arg-Pro-Gly Chemical compound NC(N)=NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O HGKHPCFTRQDHCU-IUCAKERBSA-N 0.000 description 1
- YCYXHLZRUSJITQ-SRVKXCTJSA-N Arg-Pro-Pro Chemical compound NC(=N)NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 YCYXHLZRUSJITQ-SRVKXCTJSA-N 0.000 description 1
- URAUIUGLHBRPMF-NAKRPEOUSA-N Arg-Ser-Ile Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O URAUIUGLHBRPMF-NAKRPEOUSA-N 0.000 description 1
- QMQZYILAWUOLPV-JYJNAYRXSA-N Arg-Tyr-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)CC1=CC=C(O)C=C1 QMQZYILAWUOLPV-JYJNAYRXSA-N 0.000 description 1
- VLIJAPRTSXSGFY-STQMWFEESA-N Arg-Tyr-Gly Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@H](C(=O)NCC(O)=O)CC1=CC=C(O)C=C1 VLIJAPRTSXSGFY-STQMWFEESA-N 0.000 description 1
- HZPSDHRYYIORKR-WHFBIAKZSA-N Asn-Ala-Gly Chemical compound OC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](N)CC(N)=O HZPSDHRYYIORKR-WHFBIAKZSA-N 0.000 description 1
- DMLSCRJBWUEALP-LAEOZQHASA-N Asn-Glu-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O DMLSCRJBWUEALP-LAEOZQHASA-N 0.000 description 1
- PNHQRQTVBRDIEF-CIUDSAMLSA-N Asn-Leu-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)N)N PNHQRQTVBRDIEF-CIUDSAMLSA-N 0.000 description 1
- UHGUKCOQUNPSKK-CIUDSAMLSA-N Asn-Leu-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CC(=O)N)N UHGUKCOQUNPSKK-CIUDSAMLSA-N 0.000 description 1
- BZWRLDPIWKOVKB-ZPFDUUQYSA-N Asn-Leu-Ile Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O BZWRLDPIWKOVKB-ZPFDUUQYSA-N 0.000 description 1
- YRTOMUMWSTUQAX-FXQIFTODSA-N Asn-Pro-Asp Chemical compound NC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O YRTOMUMWSTUQAX-FXQIFTODSA-N 0.000 description 1
- XMHFCUKJRCQXGI-CIUDSAMLSA-N Asn-Pro-Gln Chemical compound C1C[C@H](N(C1)C(=O)[C@H](CC(=O)N)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O XMHFCUKJRCQXGI-CIUDSAMLSA-N 0.000 description 1
- GMUOCGCDOYYWPD-FXQIFTODSA-N Asn-Pro-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O GMUOCGCDOYYWPD-FXQIFTODSA-N 0.000 description 1
- HNXWVVHIGTZTBO-LKXGYXEUSA-N Asn-Ser-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O HNXWVVHIGTZTBO-LKXGYXEUSA-N 0.000 description 1
- BCADFFUQHIMQAA-KKHAAJSZSA-N Asn-Thr-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O BCADFFUQHIMQAA-KKHAAJSZSA-N 0.000 description 1
- LMIWYCWRJVMAIQ-NHCYSSNCSA-N Asn-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)N)N LMIWYCWRJVMAIQ-NHCYSSNCSA-N 0.000 description 1
- WSWYMRLTJVKRCE-ZLUOBGJFSA-N Asp-Ala-Asp Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(O)=O WSWYMRLTJVKRCE-ZLUOBGJFSA-N 0.000 description 1
- OERMIMJQPQUIPK-FXQIFTODSA-N Asp-Arg-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(O)=O OERMIMJQPQUIPK-FXQIFTODSA-N 0.000 description 1
- AXXCUABIFZPKPM-BQBZGAKWSA-N Asp-Arg-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(O)=O AXXCUABIFZPKPM-BQBZGAKWSA-N 0.000 description 1
- ZELQAFZSJOBEQS-ACZMJKKPSA-N Asp-Asn-Glu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O ZELQAFZSJOBEQS-ACZMJKKPSA-N 0.000 description 1
- PXLNPFOJZQMXAT-BYULHYEWSA-N Asp-Asp-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O PXLNPFOJZQMXAT-BYULHYEWSA-N 0.000 description 1
- WLKVEEODTPQPLI-ACZMJKKPSA-N Asp-Gln-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O WLKVEEODTPQPLI-ACZMJKKPSA-N 0.000 description 1
- QCLHLXDWRKOHRR-GUBZILKMSA-N Asp-Glu-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)N QCLHLXDWRKOHRR-GUBZILKMSA-N 0.000 description 1
- DGKCOYGQLNWNCJ-ACZMJKKPSA-N Asp-Glu-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O DGKCOYGQLNWNCJ-ACZMJKKPSA-N 0.000 description 1
- SNDBKTFJWVEVPO-WHFBIAKZSA-N Asp-Gly-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(O)=O SNDBKTFJWVEVPO-WHFBIAKZSA-N 0.000 description 1
- LBFYTUPYYZENIR-GHCJXIJMSA-N Asp-Ile-Cys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CC(=O)O)N LBFYTUPYYZENIR-GHCJXIJMSA-N 0.000 description 1
- UMHUHHJMEXNSIV-CIUDSAMLSA-N Asp-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(O)=O UMHUHHJMEXNSIV-CIUDSAMLSA-N 0.000 description 1
- IMGLJMRIAFKUPZ-FXQIFTODSA-N Asp-Met-Cys Chemical compound CSCC[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CC(=O)O)N IMGLJMRIAFKUPZ-FXQIFTODSA-N 0.000 description 1
- RNAQPBOOJRDICC-BPUTZDHNSA-N Asp-Met-Trp Chemical compound CSCC[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CC(=O)O)N RNAQPBOOJRDICC-BPUTZDHNSA-N 0.000 description 1
- AHWRSSLYSGLBGD-CIUDSAMLSA-N Asp-Pro-Glu Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O AHWRSSLYSGLBGD-CIUDSAMLSA-N 0.000 description 1
- CUQDCPXNZPDYFQ-ZLUOBGJFSA-N Asp-Ser-Asp Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O CUQDCPXNZPDYFQ-ZLUOBGJFSA-N 0.000 description 1
- NBKLEMWHDLAUEM-CIUDSAMLSA-N Asp-Ser-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)N NBKLEMWHDLAUEM-CIUDSAMLSA-N 0.000 description 1
- DRCOAZZDQRCGGP-GHCJXIJMSA-N Asp-Ser-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O DRCOAZZDQRCGGP-GHCJXIJMSA-N 0.000 description 1
- PLOKOIJSGCISHE-BYULHYEWSA-N Asp-Val-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O PLOKOIJSGCISHE-BYULHYEWSA-N 0.000 description 1
- UXRVDHVARNBOIO-QSFUFRPTSA-N Asp-Val-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(=O)O)N UXRVDHVARNBOIO-QSFUFRPTSA-N 0.000 description 1
- QPDUWAUSSWGJSB-NGZCFLSTSA-N Asp-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC(=O)O)N QPDUWAUSSWGJSB-NGZCFLSTSA-N 0.000 description 1
- XSQAWJCVYDEWPT-GUBZILKMSA-N Cys-Met-Arg Chemical compound SC[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@H](C(O)=O)CCCN=C(N)N XSQAWJCVYDEWPT-GUBZILKMSA-N 0.000 description 1
- NRVQLLDIJJEIIZ-VZFHVOOUSA-N Cys-Thr-Ala Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](C)C(=O)O)NC(=O)[C@H](CS)N)O NRVQLLDIJJEIIZ-VZFHVOOUSA-N 0.000 description 1
- JTEGHEWKBCTIAL-IXOXFDKPSA-N Cys-Thr-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)NC(=O)[C@H](CS)N)O JTEGHEWKBCTIAL-IXOXFDKPSA-N 0.000 description 1
- PXEGEYISOXISDV-XIRDDKMYSA-N Cys-Trp-Lys Chemical compound C1=CC=C2C(C[C@@H](C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@@H](N)CS)=CNC2=C1 PXEGEYISOXISDV-XIRDDKMYSA-N 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- HHWQMFIGMMOVFK-WDSKDSINSA-N Gln-Ala-Gly Chemical compound OC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](N)CCC(N)=O HHWQMFIGMMOVFK-WDSKDSINSA-N 0.000 description 1
- KVYVOGYEMPEXBT-GUBZILKMSA-N Gln-Ala-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCC(N)=O KVYVOGYEMPEXBT-GUBZILKMSA-N 0.000 description 1
- WMOMPXKOKASNBK-PEFMBERDSA-N Gln-Asn-Ile Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O WMOMPXKOKASNBK-PEFMBERDSA-N 0.000 description 1
- CITDWMLWXNUQKD-FXQIFTODSA-N Gln-Gln-Asn Chemical compound C(CC(=O)N)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)O)N CITDWMLWXNUQKD-FXQIFTODSA-N 0.000 description 1
- PKVWNYGXMNWJSI-CIUDSAMLSA-N Gln-Gln-Gln Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O PKVWNYGXMNWJSI-CIUDSAMLSA-N 0.000 description 1
- DOQUICBEISTQHE-CIUDSAMLSA-N Gln-Pro-Asp Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O DOQUICBEISTQHE-CIUDSAMLSA-N 0.000 description 1
- OGMQXTXGLDNBSS-FXQIFTODSA-N Glu-Ala-Gln Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(O)=O OGMQXTXGLDNBSS-FXQIFTODSA-N 0.000 description 1
- LKDIBBOKUAASNP-FXQIFTODSA-N Glu-Ala-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O LKDIBBOKUAASNP-FXQIFTODSA-N 0.000 description 1
- LXAUHIRMWXQRKI-XHNCKOQMSA-N Glu-Asn-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCC(=O)O)N)C(=O)O LXAUHIRMWXQRKI-XHNCKOQMSA-N 0.000 description 1
- PCBBLFVHTYNQGG-LAEOZQHASA-N Glu-Asn-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCC(=O)O)N PCBBLFVHTYNQGG-LAEOZQHASA-N 0.000 description 1
- XHUCVVHRLNPZSZ-CIUDSAMLSA-N Glu-Gln-Glu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O XHUCVVHRLNPZSZ-CIUDSAMLSA-N 0.000 description 1
- WLIPTFCZLHCNFD-LPEHRKFASA-N Glu-Gln-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCC(=O)O)N)C(=O)O WLIPTFCZLHCNFD-LPEHRKFASA-N 0.000 description 1
- CGOHAEBMDSEKFB-FXQIFTODSA-N Glu-Glu-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(O)=O CGOHAEBMDSEKFB-FXQIFTODSA-N 0.000 description 1
- NKLRYVLERDYDBI-FXQIFTODSA-N Glu-Glu-Asp Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O NKLRYVLERDYDBI-FXQIFTODSA-N 0.000 description 1
- PHONAZGUEGIOEM-GLLZPBPUSA-N Glu-Glu-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PHONAZGUEGIOEM-GLLZPBPUSA-N 0.000 description 1
- AIGROOHQXCACHL-WDSKDSINSA-N Glu-Gly-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](C)C(O)=O AIGROOHQXCACHL-WDSKDSINSA-N 0.000 description 1
- OGNJZUXUTPQVBR-BQBZGAKWSA-N Glu-Gly-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O OGNJZUXUTPQVBR-BQBZGAKWSA-N 0.000 description 1
- OAGVHWYIBZMWLA-YFKPBYRVSA-N Glu-Gly-Gly Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)NCC(O)=O OAGVHWYIBZMWLA-YFKPBYRVSA-N 0.000 description 1
- LRPXYSGPOBVBEH-IUCAKERBSA-N Glu-Gly-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(O)=O LRPXYSGPOBVBEH-IUCAKERBSA-N 0.000 description 1
- ZWQVYZXPYSYPJD-RYUDHWBXSA-N Glu-Gly-Phe Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 ZWQVYZXPYSYPJD-RYUDHWBXSA-N 0.000 description 1
- WVTIBGWZUMJBFY-GUBZILKMSA-N Glu-His-Ser Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(O)=O WVTIBGWZUMJBFY-GUBZILKMSA-N 0.000 description 1
- ZWABFSSWTSAMQN-KBIXCLLPSA-N Glu-Ile-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O ZWABFSSWTSAMQN-KBIXCLLPSA-N 0.000 description 1
- OCJRHJZKGGSPRW-IUCAKERBSA-N Glu-Lys-Gly Chemical compound NCCCC[C@@H](C(=O)NCC(O)=O)NC(=O)[C@@H](N)CCC(O)=O OCJRHJZKGGSPRW-IUCAKERBSA-N 0.000 description 1
- ILWHFUZZCFYSKT-AVGNSLFASA-N Glu-Lys-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O ILWHFUZZCFYSKT-AVGNSLFASA-N 0.000 description 1
- QNJNPKSWAHPYGI-JYJNAYRXSA-N Glu-Phe-Leu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(O)=O)CC1=CC=CC=C1 QNJNPKSWAHPYGI-JYJNAYRXSA-N 0.000 description 1
- ALMBZBOCGSVSAI-ACZMJKKPSA-N Glu-Ser-Asn Chemical compound C(CC(=O)O)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)N)C(=O)O)N ALMBZBOCGSVSAI-ACZMJKKPSA-N 0.000 description 1
- QOXDAWODGSIDDI-GUBZILKMSA-N Glu-Ser-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)O)N QOXDAWODGSIDDI-GUBZILKMSA-N 0.000 description 1
- QXUPRMQJDWJDFR-NRPADANISA-N Glu-Val-Ser Chemical compound CC(C)[C@H](NC(=O)[C@@H](N)CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O QXUPRMQJDWJDFR-NRPADANISA-N 0.000 description 1
- OGCIHJPYKVSMTE-YUMQZZPRSA-N Gly-Arg-Glu Chemical compound [H]NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O OGCIHJPYKVSMTE-YUMQZZPRSA-N 0.000 description 1
- OCQUNKSFDYDXBG-QXEWZRGKSA-N Gly-Arg-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCN=C(N)N OCQUNKSFDYDXBG-QXEWZRGKSA-N 0.000 description 1
- KFMBRBPXHVMDFN-UWVGGRQHSA-N Gly-Arg-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCNC(N)=N KFMBRBPXHVMDFN-UWVGGRQHSA-N 0.000 description 1
- WJZLEENECIOOSA-WDSKDSINSA-N Gly-Asn-Gln Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)O WJZLEENECIOOSA-WDSKDSINSA-N 0.000 description 1
- BGVYNAQWHSTTSP-BYULHYEWSA-N Gly-Asn-Ile Chemical compound [H]NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O BGVYNAQWHSTTSP-BYULHYEWSA-N 0.000 description 1
- GRIRDMVMJJDZKV-RCOVLWMOSA-N Gly-Asn-Val Chemical compound [H]NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O GRIRDMVMJJDZKV-RCOVLWMOSA-N 0.000 description 1
- TZOVVRJYUDETQG-RCOVLWMOSA-N Gly-Asp-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CN TZOVVRJYUDETQG-RCOVLWMOSA-N 0.000 description 1
- YDWZGVCXMVLDQH-WHFBIAKZSA-N Gly-Cys-Asn Chemical compound NCC(=O)N[C@@H](CS)C(=O)N[C@H](C(O)=O)CC(N)=O YDWZGVCXMVLDQH-WHFBIAKZSA-N 0.000 description 1
- SOEATRRYCIPEHA-BQBZGAKWSA-N Gly-Glu-Glu Chemical compound [H]NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O SOEATRRYCIPEHA-BQBZGAKWSA-N 0.000 description 1
- JUBDONGMHASUCN-IUCAKERBSA-N Gly-Glu-His Chemical compound NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](Cc1cnc[nH]1)C(O)=O JUBDONGMHASUCN-IUCAKERBSA-N 0.000 description 1
- UFPXDFOYHVEIPI-BYPYZUCNSA-N Gly-Gly-Asp Chemical compound NCC(=O)NCC(=O)N[C@H](C(O)=O)CC(O)=O UFPXDFOYHVEIPI-BYPYZUCNSA-N 0.000 description 1
- FSPVILZGHUJOHS-QWRGUYRKSA-N Gly-His-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CNC=N1 FSPVILZGHUJOHS-QWRGUYRKSA-N 0.000 description 1
- LIXWIUAORXJNBH-QWRGUYRKSA-N Gly-Leu-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)CN LIXWIUAORXJNBH-QWRGUYRKSA-N 0.000 description 1
- GMTXWRIDLGTVFC-IUCAKERBSA-N Gly-Lys-Glu Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O GMTXWRIDLGTVFC-IUCAKERBSA-N 0.000 description 1
- FXGRXIATVXUAHO-WEDXCCLWSA-N Gly-Lys-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCCN FXGRXIATVXUAHO-WEDXCCLWSA-N 0.000 description 1
- POJJAZJHBGXEGM-YUMQZZPRSA-N Gly-Ser-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)CN POJJAZJHBGXEGM-YUMQZZPRSA-N 0.000 description 1
- ABPRMMYHROQBLY-NKWVEPMBSA-N Gly-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)CN)C(=O)O ABPRMMYHROQBLY-NKWVEPMBSA-N 0.000 description 1
- RHRLHXQWHCNJKR-PMVVWTBXSA-N Gly-Thr-His Chemical compound NCC(=O)N[C@@H]([C@H](O)C)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 RHRLHXQWHCNJKR-PMVVWTBXSA-N 0.000 description 1
- BXDLTKLPPKBVEL-FJXKBIBVSA-N Gly-Thr-Met Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(O)=O BXDLTKLPPKBVEL-FJXKBIBVSA-N 0.000 description 1
- CUVBTVWFVIIDOC-YEPSODPASA-N Gly-Thr-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)CN CUVBTVWFVIIDOC-YEPSODPASA-N 0.000 description 1
- DNVDEMWIYLVIQU-RCOVLWMOSA-N Gly-Val-Asp Chemical compound NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O DNVDEMWIYLVIQU-RCOVLWMOSA-N 0.000 description 1
- JWLWNCVBBSBCEM-NKIYYHGXSA-N His-Gln-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC1=CN=CN1)N)O JWLWNCVBBSBCEM-NKIYYHGXSA-N 0.000 description 1
- MPXGJGBXCRQQJE-MXAVVETBSA-N His-Ile-Leu Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O MPXGJGBXCRQQJE-MXAVVETBSA-N 0.000 description 1
- JUIOPCXACJLRJK-AVGNSLFASA-N His-Lys-Glu Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N JUIOPCXACJLRJK-AVGNSLFASA-N 0.000 description 1
- QICVAHODWHIWIS-HTFCKZLJSA-N Ile-Ala-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)O)N QICVAHODWHIWIS-HTFCKZLJSA-N 0.000 description 1
- DMHGKBGOUAJRHU-RVMXOQNASA-N Ile-Arg-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N1CCC[C@@H]1C(=O)O)N DMHGKBGOUAJRHU-RVMXOQNASA-N 0.000 description 1
- DMHGKBGOUAJRHU-UHFFFAOYSA-N Ile-Arg-Pro Natural products CCC(C)C(N)C(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(O)=O DMHGKBGOUAJRHU-UHFFFAOYSA-N 0.000 description 1
- NCSIQAFSIPHVAN-IUKAMOBKSA-N Ile-Asn-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N NCSIQAFSIPHVAN-IUKAMOBKSA-N 0.000 description 1
- NYEYYMLUABXDMC-NHCYSSNCSA-N Ile-Gly-Leu Chemical compound CC[C@H](C)[C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)O)N NYEYYMLUABXDMC-NHCYSSNCSA-N 0.000 description 1
- HYLIOBDWPQNLKI-HVTMNAMFSA-N Ile-His-Gln Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N HYLIOBDWPQNLKI-HVTMNAMFSA-N 0.000 description 1
- UWLHDGMRWXHFFY-HPCHECBXSA-N Ile-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1CCC[C@@H]1C(=O)O)N UWLHDGMRWXHFFY-HPCHECBXSA-N 0.000 description 1
- SAVXZJYTTQQQDD-QEWYBTABSA-N Ile-Phe-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N SAVXZJYTTQQQDD-QEWYBTABSA-N 0.000 description 1
- VGSPNSSCMOHRRR-BJDJZHNGSA-N Ile-Ser-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O)N VGSPNSSCMOHRRR-BJDJZHNGSA-N 0.000 description 1
- JNLSTRPWUXOORL-MMWGEVLESA-N Ile-Ser-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N JNLSTRPWUXOORL-MMWGEVLESA-N 0.000 description 1
- WCNWGAUZWWSYDG-SVSWQMSJSA-N Ile-Thr-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)O)N WCNWGAUZWWSYDG-SVSWQMSJSA-N 0.000 description 1
- RQZFWBLDTBDEOF-RNJOBUHISA-N Ile-Val-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N RQZFWBLDTBDEOF-RNJOBUHISA-N 0.000 description 1
- FADYJNXDPBKVCA-UHFFFAOYSA-N L-Phenylalanyl-L-lysin Natural products NCCCCC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FADYJNXDPBKVCA-UHFFFAOYSA-N 0.000 description 1
- RCFDOSNHHZGBOY-UHFFFAOYSA-N L-isoleucyl-L-alanine Natural products CCC(C)C(N)C(=O)NC(C)C(O)=O RCFDOSNHHZGBOY-UHFFFAOYSA-N 0.000 description 1
- TYYLDKGBCJGJGW-UHFFFAOYSA-N L-tryptophan-L-tyrosine Natural products C=1NC2=CC=CC=C2C=1CC(N)C(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 TYYLDKGBCJGJGW-UHFFFAOYSA-N 0.000 description 1
- LZDNBBYBDGBADK-UHFFFAOYSA-N L-valyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)C(C)C)C(O)=O)=CNC2=C1 LZDNBBYBDGBADK-UHFFFAOYSA-N 0.000 description 1
- 241000880493 Leptailurus serval Species 0.000 description 1
- KWTVLKBOQATPHJ-SRVKXCTJSA-N Leu-Ala-Lys Chemical compound C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(C)C)N KWTVLKBOQATPHJ-SRVKXCTJSA-N 0.000 description 1
- YOZCKMXHBYKOMQ-IHRRRGAJSA-N Leu-Arg-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCCN)C(=O)O)N YOZCKMXHBYKOMQ-IHRRRGAJSA-N 0.000 description 1
- TWQIYNGNYNJUFM-NHCYSSNCSA-N Leu-Asn-Val Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O TWQIYNGNYNJUFM-NHCYSSNCSA-N 0.000 description 1
- QCSFMCFHVGTLFF-NHCYSSNCSA-N Leu-Asp-Val Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O QCSFMCFHVGTLFF-NHCYSSNCSA-N 0.000 description 1
- RRSLQOLASISYTB-CIUDSAMLSA-N Leu-Cys-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(O)=O)C(O)=O RRSLQOLASISYTB-CIUDSAMLSA-N 0.000 description 1
- QDSKNVXKLPQNOJ-GVXVVHGQSA-N Leu-Gln-Val Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O QDSKNVXKLPQNOJ-GVXVVHGQSA-N 0.000 description 1
- PRZVBIAOPFGAQF-SRVKXCTJSA-N Leu-Glu-Met Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(O)=O PRZVBIAOPFGAQF-SRVKXCTJSA-N 0.000 description 1
- ZFNLIDNJUWNIJL-WDCWCFNPSA-N Leu-Glu-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O ZFNLIDNJUWNIJL-WDCWCFNPSA-N 0.000 description 1
- HYIFFZAQXPUEAU-QWRGUYRKSA-N Leu-Gly-Leu Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(C)C HYIFFZAQXPUEAU-QWRGUYRKSA-N 0.000 description 1
- VGPCJSXPPOQPBK-YUMQZZPRSA-N Leu-Gly-Ser Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O VGPCJSXPPOQPBK-YUMQZZPRSA-N 0.000 description 1
- VZBIUJURDLFFOE-IHRRRGAJSA-N Leu-His-Arg Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O VZBIUJURDLFFOE-IHRRRGAJSA-N 0.000 description 1
- HNDWYLYAYNBWMP-AJNGGQMLSA-N Leu-Ile-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(C)C)N HNDWYLYAYNBWMP-AJNGGQMLSA-N 0.000 description 1
- QNBVTHNJGCOVFA-AVGNSLFASA-N Leu-Leu-Glu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCC(O)=O QNBVTHNJGCOVFA-AVGNSLFASA-N 0.000 description 1
- XVZCXCTYGHPNEM-UHFFFAOYSA-N Leu-Leu-Pro Natural products CC(C)CC(N)C(=O)NC(CC(C)C)C(=O)N1CCCC1C(O)=O XVZCXCTYGHPNEM-UHFFFAOYSA-N 0.000 description 1
- FKQPWMZLIIATBA-AJNGGQMLSA-N Leu-Lys-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O FKQPWMZLIIATBA-AJNGGQMLSA-N 0.000 description 1
- LZHJZLHSRGWBBE-IHRRRGAJSA-N Leu-Lys-Val Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O LZHJZLHSRGWBBE-IHRRRGAJSA-N 0.000 description 1
- RRVCZCNFXIFGRA-DCAQKATOSA-N Leu-Pro-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O RRVCZCNFXIFGRA-DCAQKATOSA-N 0.000 description 1
- VULJUQZPSOASBZ-SRVKXCTJSA-N Leu-Pro-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O VULJUQZPSOASBZ-SRVKXCTJSA-N 0.000 description 1
- JLYUZRKPDKHUTC-WDSOQIARSA-N Leu-Pro-Trp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O JLYUZRKPDKHUTC-WDSOQIARSA-N 0.000 description 1
- BRTVHXHCUSXYRI-CIUDSAMLSA-N Leu-Ser-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O BRTVHXHCUSXYRI-CIUDSAMLSA-N 0.000 description 1
- FBNPMTNBFFAMMH-AVGNSLFASA-N Leu-Val-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N FBNPMTNBFFAMMH-AVGNSLFASA-N 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- JGAMUXDWYSXYLM-SRVKXCTJSA-N Lys-Arg-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O JGAMUXDWYSXYLM-SRVKXCTJSA-N 0.000 description 1
- VHNOAIFVYUQOOY-XUXIUFHCSA-N Lys-Arg-Ile Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VHNOAIFVYUQOOY-XUXIUFHCSA-N 0.000 description 1
- CKSXSQUVEYCDIW-AVGNSLFASA-N Lys-Arg-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCCN)N CKSXSQUVEYCDIW-AVGNSLFASA-N 0.000 description 1
- LZWNAOIMTLNMDW-NHCYSSNCSA-N Lys-Asn-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCCCN)N LZWNAOIMTLNMDW-NHCYSSNCSA-N 0.000 description 1
- KWUKZRFFKPLUPE-HJGDQZAQSA-N Lys-Asp-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KWUKZRFFKPLUPE-HJGDQZAQSA-N 0.000 description 1
- ULUQBUKAPDUKOC-GVXVVHGQSA-N Lys-Glu-Val Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O ULUQBUKAPDUKOC-GVXVVHGQSA-N 0.000 description 1
- PRSBSVAVOQOAMI-BJDJZHNGSA-N Lys-Ile-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H](N)CCCCN PRSBSVAVOQOAMI-BJDJZHNGSA-N 0.000 description 1
- ONPDTSFZAIWMDI-AVGNSLFASA-N Lys-Leu-Gln Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O ONPDTSFZAIWMDI-AVGNSLFASA-N 0.000 description 1
- YDDDRTIPNTWGIG-SRVKXCTJSA-N Lys-Lys-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O YDDDRTIPNTWGIG-SRVKXCTJSA-N 0.000 description 1
- SVSQSPICRKBMSZ-SRVKXCTJSA-N Lys-Pro-Gln Chemical compound [H]N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(O)=O SVSQSPICRKBMSZ-SRVKXCTJSA-N 0.000 description 1
- YTJFXEDRUOQGSP-DCAQKATOSA-N Lys-Pro-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O YTJFXEDRUOQGSP-DCAQKATOSA-N 0.000 description 1
- DIBZLYZXTSVGLN-CIUDSAMLSA-N Lys-Ser-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O DIBZLYZXTSVGLN-CIUDSAMLSA-N 0.000 description 1
- OHXUUQDOBQKSNB-AVGNSLFASA-N Lys-Val-Arg Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O OHXUUQDOBQKSNB-AVGNSLFASA-N 0.000 description 1
- RPWQJSBMXJSCPD-XUXIUFHCSA-N Lys-Val-Ile Chemical compound CC[C@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CCCCN)C(C)C)C(O)=O RPWQJSBMXJSCPD-XUXIUFHCSA-N 0.000 description 1
- OZVXDDFYCQOPFD-XQQFMLRXSA-N Lys-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCCCN)N OZVXDDFYCQOPFD-XQQFMLRXSA-N 0.000 description 1
- DSWOTZCVCBEPOU-IUCAKERBSA-N Met-Arg-Gly Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(=O)NCC(O)=O)CCCNC(N)=N DSWOTZCVCBEPOU-IUCAKERBSA-N 0.000 description 1
- DBOMZJOESVYERT-GUBZILKMSA-N Met-Asn-Met Chemical compound CSCC[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCSC)C(=O)O)N DBOMZJOESVYERT-GUBZILKMSA-N 0.000 description 1
- IHITVQKJXQQGLJ-LPEHRKFASA-N Met-Asn-Pro Chemical compound CSCC[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N1CCC[C@@H]1C(=O)O)N IHITVQKJXQQGLJ-LPEHRKFASA-N 0.000 description 1
- GODBLDDYHFTUAH-CIUDSAMLSA-N Met-Asp-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCC(O)=O GODBLDDYHFTUAH-CIUDSAMLSA-N 0.000 description 1
- VOOINLQYUZOREH-SRVKXCTJSA-N Met-Gln-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCSC)N VOOINLQYUZOREH-SRVKXCTJSA-N 0.000 description 1
- CHQWUYSNAOABIP-ZPFDUUQYSA-N Met-Glu-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCSC)N CHQWUYSNAOABIP-ZPFDUUQYSA-N 0.000 description 1
- WWWGMQHQSAUXBU-BQBZGAKWSA-N Met-Gly-Asn Chemical compound CSCC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(N)=O WWWGMQHQSAUXBU-BQBZGAKWSA-N 0.000 description 1
- UZWMJZSOXGOVIN-LURJTMIESA-N Met-Gly-Gly Chemical compound CSCC[C@H](N)C(=O)NCC(=O)NCC(O)=O UZWMJZSOXGOVIN-LURJTMIESA-N 0.000 description 1
- YYEIFXZOBZVDPH-DCAQKATOSA-N Met-Lys-Asp Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(O)=O YYEIFXZOBZVDPH-DCAQKATOSA-N 0.000 description 1
- HOZNVKDCKZPRER-XUXIUFHCSA-N Met-Lys-Ile Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O HOZNVKDCKZPRER-XUXIUFHCSA-N 0.000 description 1
- VYDLZDRMOFYOGV-TUAOUCFPSA-N Met-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCSC)N VYDLZDRMOFYOGV-TUAOUCFPSA-N 0.000 description 1
- WUGMRIBZSVSJNP-UHFFFAOYSA-N N-L-alanyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)C)C(O)=O)=CNC2=C1 WUGMRIBZSVSJNP-UHFFFAOYSA-N 0.000 description 1
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 1
- XMBSYZWANAQXEV-UHFFFAOYSA-N N-alpha-L-glutamyl-L-phenylalanine Natural products OC(=O)CCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XMBSYZWANAQXEV-UHFFFAOYSA-N 0.000 description 1
- 108010002311 N-glycylglutamic acid Proteins 0.000 description 1
- 108010066427 N-valyltryptophan Proteins 0.000 description 1
- BKWJQWJPZMUWEG-LFSVMHDDSA-N Phe-Ala-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=CC=C1 BKWJQWJPZMUWEG-LFSVMHDDSA-N 0.000 description 1
- XMPUYNHKEPFERE-IHRRRGAJSA-N Phe-Asp-Arg Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 XMPUYNHKEPFERE-IHRRRGAJSA-N 0.000 description 1
- OQTDZEJJWWAGJT-KKUMJFAQSA-N Phe-Lys-Asp Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(O)=O OQTDZEJJWWAGJT-KKUMJFAQSA-N 0.000 description 1
- FENSZYFJQOFSQR-FIRPJDEBSA-N Phe-Phe-Ile Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(O)=O)NC(=O)[C@@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FENSZYFJQOFSQR-FIRPJDEBSA-N 0.000 description 1
- AAERWTUHZKLDLC-IHRRRGAJSA-N Phe-Pro-Asp Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O AAERWTUHZKLDLC-IHRRRGAJSA-N 0.000 description 1
- WEDZFLRYSIDIRX-IHRRRGAJSA-N Phe-Ser-Arg Chemical compound NC(=N)NCCC[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CC=CC=C1 WEDZFLRYSIDIRX-IHRRRGAJSA-N 0.000 description 1
- MCIXMYKSPQUMJG-SRVKXCTJSA-N Phe-Ser-Ser Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O MCIXMYKSPQUMJG-SRVKXCTJSA-N 0.000 description 1
- QUUCAHIYARMNBL-FHWLQOOXSA-N Phe-Tyr-Gln Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N QUUCAHIYARMNBL-FHWLQOOXSA-N 0.000 description 1
- JSGWNFKWZNPDAV-YDHLFZDLSA-N Phe-Val-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CC1=CC=CC=C1 JSGWNFKWZNPDAV-YDHLFZDLSA-N 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- SSSFPISOZOLQNP-GUBZILKMSA-N Pro-Arg-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O SSSFPISOZOLQNP-GUBZILKMSA-N 0.000 description 1
- SWXSLPHTJVAWDF-VEVYYDQMSA-N Pro-Asn-Thr Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O SWXSLPHTJVAWDF-VEVYYDQMSA-N 0.000 description 1
- FEVDNIBDCRKMER-IUCAKERBSA-N Pro-Gly-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)CNC(=O)[C@@H]1CCCN1 FEVDNIBDCRKMER-IUCAKERBSA-N 0.000 description 1
- BBFRBZYKHIKFBX-GMOBBJLQSA-N Pro-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@@H]1CCCN1 BBFRBZYKHIKFBX-GMOBBJLQSA-N 0.000 description 1
- HFNPOYOKIPGAEI-SRVKXCTJSA-N Pro-Leu-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1 HFNPOYOKIPGAEI-SRVKXCTJSA-N 0.000 description 1
- DRKAXLDECUGLFE-ULQDDVLXSA-N Pro-Leu-Phe Chemical compound CC(C)C[C@H](NC(=O)[C@@H]1CCCN1)C(=O)N[C@@H](Cc1ccccc1)C(O)=O DRKAXLDECUGLFE-ULQDDVLXSA-N 0.000 description 1
- MCWHYUWXVNRXFV-RWMBFGLXSA-N Pro-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@@H]2CCCN2 MCWHYUWXVNRXFV-RWMBFGLXSA-N 0.000 description 1
- OFGUOWQVEGTVNU-DCAQKATOSA-N Pro-Lys-Ala Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O OFGUOWQVEGTVNU-DCAQKATOSA-N 0.000 description 1
- POQFNPILEQEODH-FXQIFTODSA-N Pro-Ser-Ala Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O POQFNPILEQEODH-FXQIFTODSA-N 0.000 description 1
- BXHRXLMCYSZSIY-STECZYCISA-N Pro-Tyr-Ile Chemical compound CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H]1CCCN1)C(O)=O BXHRXLMCYSZSIY-STECZYCISA-N 0.000 description 1
- ZXLUWXWISXIFIX-ACZMJKKPSA-N Ser-Asn-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O ZXLUWXWISXIFIX-ACZMJKKPSA-N 0.000 description 1
- VAUMZJHYZQXZBQ-WHFBIAKZSA-N Ser-Asn-Gly Chemical compound OC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O VAUMZJHYZQXZBQ-WHFBIAKZSA-N 0.000 description 1
- TUYBIWUZWJUZDD-ACZMJKKPSA-N Ser-Cys-Gln Chemical compound OC[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@H](C(O)=O)CCC(N)=O TUYBIWUZWJUZDD-ACZMJKKPSA-N 0.000 description 1
- OJPHFSOMBZKQKQ-GUBZILKMSA-N Ser-Gln-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CO OJPHFSOMBZKQKQ-GUBZILKMSA-N 0.000 description 1
- UQFYNFTYDHUIMI-WHFBIAKZSA-N Ser-Gly-Ala Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](N)CO UQFYNFTYDHUIMI-WHFBIAKZSA-N 0.000 description 1
- MUARUIBTKQJKFY-WHFBIAKZSA-N Ser-Gly-Asp Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O MUARUIBTKQJKFY-WHFBIAKZSA-N 0.000 description 1
- WSTIOCFMWXNOCX-YUMQZZPRSA-N Ser-Gly-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)CNC(=O)[C@H](CO)N WSTIOCFMWXNOCX-YUMQZZPRSA-N 0.000 description 1
- YIUWWXVTYLANCJ-NAKRPEOUSA-N Ser-Ile-Arg Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O YIUWWXVTYLANCJ-NAKRPEOUSA-N 0.000 description 1
- PPNPDKGQRFSCAC-CIUDSAMLSA-N Ser-Lys-Asp Chemical compound NCCCC[C@H](NC(=O)[C@@H](N)CO)C(=O)N[C@@H](CC(O)=O)C(O)=O PPNPDKGQRFSCAC-CIUDSAMLSA-N 0.000 description 1
- XUDRHBPSPAPDJP-SRVKXCTJSA-N Ser-Lys-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CO XUDRHBPSPAPDJP-SRVKXCTJSA-N 0.000 description 1
- FPCGZYMRFFIYIH-CIUDSAMLSA-N Ser-Lys-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O FPCGZYMRFFIYIH-CIUDSAMLSA-N 0.000 description 1
- HEYZPTCCEIWHRO-IHRRRGAJSA-N Ser-Met-Phe Chemical compound OC[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 HEYZPTCCEIWHRO-IHRRRGAJSA-N 0.000 description 1
- FKYWFUYPVKLJLP-DCAQKATOSA-N Ser-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO FKYWFUYPVKLJLP-DCAQKATOSA-N 0.000 description 1
- SQHKXWODKJDZRC-LKXGYXEUSA-N Ser-Thr-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(O)=O SQHKXWODKJDZRC-LKXGYXEUSA-N 0.000 description 1
- PCJLFYBAQZQOFE-KATARQTJSA-N Ser-Thr-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CO)N)O PCJLFYBAQZQOFE-KATARQTJSA-N 0.000 description 1
- OJFFAQFRCVPHNN-JYBASQMISA-N Ser-Thr-Trp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O OJFFAQFRCVPHNN-JYBASQMISA-N 0.000 description 1
- VAIWUNAAPZZGRI-IHPCNDPISA-N Ser-Trp-Phe Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)O)NC(=O)[C@H](CC2=CNC3=CC=CC=C32)NC(=O)[C@H](CO)N VAIWUNAAPZZGRI-IHPCNDPISA-N 0.000 description 1
- BEBVVQPDSHHWQL-NRPADANISA-N Ser-Val-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O BEBVVQPDSHHWQL-NRPADANISA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- LHUBVKCLOVALIA-HJGDQZAQSA-N Thr-Arg-Gln Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(O)=O LHUBVKCLOVALIA-HJGDQZAQSA-N 0.000 description 1
- MMTOHPRBJKEZHT-BWBBJGPYSA-N Thr-Cys-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(O)=O MMTOHPRBJKEZHT-BWBBJGPYSA-N 0.000 description 1
- UZJDBCHMIQXLOQ-HEIBUPTGSA-N Thr-Cys-Thr Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N)O UZJDBCHMIQXLOQ-HEIBUPTGSA-N 0.000 description 1
- QILPDQCTQZDHFM-HJGDQZAQSA-N Thr-Gln-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O QILPDQCTQZDHFM-HJGDQZAQSA-N 0.000 description 1
- VGYBYGQXZJDZJU-XQXXSGGOSA-N Thr-Glu-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(O)=O VGYBYGQXZJDZJU-XQXXSGGOSA-N 0.000 description 1
- AHOLTQCAVBSUDP-PPCPHDFISA-N Thr-Ile-Lys Chemical compound CC[C@H](C)[C@H](NC(=O)[C@@H](N)[C@@H](C)O)C(=O)N[C@@H](CCCCN)C(O)=O AHOLTQCAVBSUDP-PPCPHDFISA-N 0.000 description 1
- CJXURNZYNHCYFD-WDCWCFNPSA-N Thr-Lys-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N)O CJXURNZYNHCYFD-WDCWCFNPSA-N 0.000 description 1
- JWQNAFHCXKVZKZ-UVOCVTCTSA-N Thr-Lys-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O JWQNAFHCXKVZKZ-UVOCVTCTSA-N 0.000 description 1
- PZSDPRBZINDEJV-HTUGSXCWSA-N Thr-Phe-Gln Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(O)=O PZSDPRBZINDEJV-HTUGSXCWSA-N 0.000 description 1
- SCQBNMKLZVCXNX-ZFWWWQNUSA-N Trp-Arg-Gly Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)NCC(=O)O)N SCQBNMKLZVCXNX-ZFWWWQNUSA-N 0.000 description 1
- VEYXZZGMIBKXCN-UBHSHLNASA-N Trp-Asp-Asp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)O)N VEYXZZGMIBKXCN-UBHSHLNASA-N 0.000 description 1
- DXHHCIYKHRKBOC-BHYGNILZSA-N Trp-Gln-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC2=CNC3=CC=CC=C32)N)C(=O)O DXHHCIYKHRKBOC-BHYGNILZSA-N 0.000 description 1
- RXEQOXHCHQJMSO-IHPCNDPISA-N Trp-His-Leu Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(O)=O RXEQOXHCHQJMSO-IHPCNDPISA-N 0.000 description 1
- OWSRIUBVJOQHNY-IHPCNDPISA-N Trp-Lys-His Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC3=CN=CN3)C(=O)O)N OWSRIUBVJOQHNY-IHPCNDPISA-N 0.000 description 1
- FNWGDMZVYBVAGJ-XEGUGMAKSA-N Tyr-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)CNC(=O)[C@H](CC1=CC=C(C=C1)O)N FNWGDMZVYBVAGJ-XEGUGMAKSA-N 0.000 description 1
- SZEIFUXUTBBQFQ-STQMWFEESA-N Tyr-Pro-Gly Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O SZEIFUXUTBBQFQ-STQMWFEESA-N 0.000 description 1
- QVYFTFIBKCDHIE-ACRUOGEOSA-N Tyr-Tyr-Lys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)N[C@@H](CCCCN)C(=O)O)N)O QVYFTFIBKCDHIE-ACRUOGEOSA-N 0.000 description 1
- UUJHRSTVQCFDPA-UFYCRDLUSA-N Tyr-Tyr-Val Chemical compound C([C@@H](C(=O)N[C@@H](C(C)C)C(O)=O)NC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 UUJHRSTVQCFDPA-UFYCRDLUSA-N 0.000 description 1
- ZMDCGGKHRKNWKD-LAEOZQHASA-N Val-Asn-Glu Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N ZMDCGGKHRKNWKD-LAEOZQHASA-N 0.000 description 1
- DDNIHOWRDOXXPF-NGZCFLSTSA-N Val-Asp-Pro Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@@H]1C(=O)O)N DDNIHOWRDOXXPF-NGZCFLSTSA-N 0.000 description 1
- QHFQQRKNGCXTHL-AUTRQRHGSA-N Val-Gln-Glu Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O QHFQQRKNGCXTHL-AUTRQRHGSA-N 0.000 description 1
- CVIXTAITYJQMPE-LAEOZQHASA-N Val-Glu-Asn Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O CVIXTAITYJQMPE-LAEOZQHASA-N 0.000 description 1
- VVZDBPBZHLQPPB-XVKPBYJWSA-N Val-Glu-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O VVZDBPBZHLQPPB-XVKPBYJWSA-N 0.000 description 1
- WFENBJPLZMPVAX-XVKPBYJWSA-N Val-Gly-Glu Chemical compound CC(C)[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCC(O)=O WFENBJPLZMPVAX-XVKPBYJWSA-N 0.000 description 1
- APEBUJBRGCMMHP-HJWJTTGWSA-N Val-Ile-Phe Chemical compound CC(C)[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 APEBUJBRGCMMHP-HJWJTTGWSA-N 0.000 description 1
- HGJRMXOWUWVUOA-GVXVVHGQSA-N Val-Leu-Gln Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](C(C)C)N HGJRMXOWUWVUOA-GVXVVHGQSA-N 0.000 description 1
- LYERIXUFCYVFFX-GVXVVHGQSA-N Val-Leu-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N LYERIXUFCYVFFX-GVXVVHGQSA-N 0.000 description 1
- MJFSRZZJQWZHFQ-SRVKXCTJSA-N Val-Met-Val Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(=O)O)N MJFSRZZJQWZHFQ-SRVKXCTJSA-N 0.000 description 1
- LGXUZJIQCGXKGZ-QXEWZRGKSA-N Val-Pro-Asn Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)N)C(=O)O)N LGXUZJIQCGXKGZ-QXEWZRGKSA-N 0.000 description 1
- HPOSMQWRPMRMFO-GUBZILKMSA-N Val-Pro-Cys Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CS)C(=O)O)N HPOSMQWRPMRMFO-GUBZILKMSA-N 0.000 description 1
- QZKVWWIUSQGWMY-IHRRRGAJSA-N Val-Ser-Phe Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 QZKVWWIUSQGWMY-IHRRRGAJSA-N 0.000 description 1
- GBIUHAYJGWVNLN-UHFFFAOYSA-N Val-Ser-Pro Natural products CC(C)C(N)C(=O)NC(CO)C(=O)N1CCCC1C(O)=O GBIUHAYJGWVNLN-UHFFFAOYSA-N 0.000 description 1
- MNSSBIHFEUUXNW-RCWTZXSCSA-N Val-Thr-Arg Chemical compound CC(C)[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N MNSSBIHFEUUXNW-RCWTZXSCSA-N 0.000 description 1
- PQSNETRGCRUOGP-KKHAAJSZSA-N Val-Thr-Asn Chemical compound CC(C)[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CC(N)=O PQSNETRGCRUOGP-KKHAAJSZSA-N 0.000 description 1
- IECQJCJNPJVUSB-IHRRRGAJSA-N Val-Tyr-Ser Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CO)C(O)=O IECQJCJNPJVUSB-IHRRRGAJSA-N 0.000 description 1
- 108010076324 alanyl-glycyl-glycine Proteins 0.000 description 1
- 108010069020 alanyl-prolyl-glycine Proteins 0.000 description 1
- 108010041407 alanylaspartic acid Proteins 0.000 description 1
- 108010005233 alanylglutamic acid Proteins 0.000 description 1
- 108010087924 alanylproline Proteins 0.000 description 1
- 108010013835 arginine glutamate Proteins 0.000 description 1
- 108010069926 arginyl-glycyl-serine Proteins 0.000 description 1
- 108010038850 arginyl-isoleucyl-tyrosine Proteins 0.000 description 1
- 108010029539 arginyl-prolyl-proline Proteins 0.000 description 1
- 108010094001 arginyl-tryptophyl-arginine Proteins 0.000 description 1
- 108010062796 arginyllysine Proteins 0.000 description 1
- 108010010430 asparagine-proline-alanine Proteins 0.000 description 1
- 108010092854 aspartyllysine Proteins 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- FSXRLASFHBWESK-UHFFFAOYSA-N dipeptide phenylalanyl-tyrosine Natural products C=1C=C(O)C=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FSXRLASFHBWESK-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000000408 embryogenic effect Effects 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 108010006664 gamma-glutamyl-glycyl-glycine Proteins 0.000 description 1
- 108010078144 glutaminyl-glycine Proteins 0.000 description 1
- 108010008237 glutamyl-valyl-glycine Proteins 0.000 description 1
- VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 description 1
- JYPCXBJRLBHWME-UHFFFAOYSA-N glycyl-L-prolyl-L-arginine Natural products NCC(=O)N1CCCC1C(=O)NC(CCCN=C(N)N)C(O)=O JYPCXBJRLBHWME-UHFFFAOYSA-N 0.000 description 1
- 108010075431 glycyl-alanyl-phenylalanine Proteins 0.000 description 1
- 108010072405 glycyl-aspartyl-glycine Proteins 0.000 description 1
- 108010062266 glycyl-glycyl-argininal Proteins 0.000 description 1
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 1
- 108010050848 glycylleucine Proteins 0.000 description 1
- 108010015792 glycyllysine Proteins 0.000 description 1
- 108010037850 glycylvaline Proteins 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 108010060857 isoleucyl-valyl-tyrosine Proteins 0.000 description 1
- 108010083708 leucyl-aspartyl-valine Proteins 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 108010018625 phenylalanylarginine Proteins 0.000 description 1
- 108010073025 phenylalanylphenylalanine Proteins 0.000 description 1
- 108010004914 prolylarginine Proteins 0.000 description 1
- 108010070643 prolylglutamic acid Proteins 0.000 description 1
- 108010029020 prolylglycine Proteins 0.000 description 1
- 108010090894 prolylleucine Proteins 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 108010048397 seryl-lysyl-leucine Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 108010071097 threonyl-lysyl-proline Proteins 0.000 description 1
- 108010072986 threonyl-seryl-lysine Proteins 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 108010044292 tryptophyltyrosine Proteins 0.000 description 1
- 108010015385 valyl-prolyl-proline Proteins 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明公开了Os516蛋白及其编码基因和应用。本发明提供的蛋白质，命名为Os516蛋白，为序列表中序列1所示的蛋白质。编码Os516蛋白的DNA分子(即Os516基因)也属于本发明的保护范围。本发明还保护一种制备转基因植物的方法，包括如下步骤：在受体植物中导入抑制Os516基因表达的物质，得到转基因植物；与所述受体植物相比，所述转基因植物的株高增高和/或穗长增长。本发明还保护一种植物育种方法，包括如下步骤：对受体植物中的Os516基因进行基因编辑，得到基因编辑植株；与所述受体植物相比，所述基因编辑植株的株高增高和/或穗长增长。本发明可用于植物的株高/穗长性状改良，对于植物育种特别是水稻育种，具有重大的应用推广价值。
Description
技术领域
本发明涉及Os516蛋白及其编码基因和应用。
背景技术
水稻原产于中国和印度。是世界主要粮食作物之一。中国水稻播种面占全国粮食作物的1/4，而产量则占一半以上。栽培历史已有14000～18000年。为重要粮食作物；除食用颖果外，可制淀粉、酿酒、制醋，米糠可制糖、榨油、提取糠醛，供工业及医药用；稻秆为良好饲料及造纸原料和编织材料，谷芽和稻根可供药用。
近年来，生物技术的迅速发展大大地推动了植物育种研究手段的革新与研究水平的不断提高，植物抗病虫、抗除草剂生物技术育种已开始进入实用化阶段。利用生物技术手段将外源杀虫、抗除草剂基因导入植物基因组内，打破了植物种属甚至物种之间难以杂交的天然屏障，实现了抗虫、抗除草剂基因的转移，从而使植物迅速地、定向地获得抗虫性及机械化除草，同时又能保留原有的良好农艺性状。由于转基因玉米的每株植株都具有相当程度的抗性，因而其抗虫、抗除草剂效果比人工防治防治效果要好且稳定，还能够节省人力和物力的投入，有效的节约社会资源。其他农艺性状改良转基因植物进展及应用不如抗虫、抗除草剂性状理想，主要是由于没有优良的性状改良基因，大多农艺性状主要是有一些微效的多基因控制所致，一直没有理想的基因进行操作。
发明内容
本发明的目的是提供Os516蛋白及其编码基因和应用。
本发明提供的蛋白质，命名为Os516蛋白，获自水稻(Oryza sativa)，为如下(a1)或(a2)或(a3)或(a4)：
(a1)序列表中序列1所示的蛋白质；
(a2)在(a1)所述蛋白质的N端或/和C端连接标签得到的融合蛋白；
(a3)将(a1)经过一个或几个氨基酸残基的取代和/或缺失和/或添加得到的与植物的株高和/或穗长相关的蛋白质；
(a4)来源于水稻且与(a1)具有98％以上同一性且与植物株高的和/或穗长相关的蛋白质。
标签具体如表1所示。
表1标签的序列
编码Os516蛋白的核酸分子也属于本发明的保护范围。
编码Os516蛋白的核酸分子具体可为RNA分子或DNA分子。
编码Os516蛋白的DNA分子，即Os516基因。
编码Os516蛋白的RNA分子，即Os516基因转录得到的RNA分子。
所述Os516基因为如下(b1)或(b2)或(b3)或(b4)：
(b1)编码区如序列表中序列2所示的DNA分子；
(b2)序列表中序列3所示的DNA分子；
(b3)来源于水稻且与(b1)或(b2)具有95％以上同一性且编码所述蛋白质的DNA分子；
(b4)在严格条件下与(b1)或(b2)限定的核苷酸序列杂交且编码所述蛋白质的DNA分子。
上述严格条件可为用6×SSC，0.5％SDS的溶液，在65℃下杂交，然后用2×SSC，0.1％SDS和1×SSC，0.1％SDS各洗膜一次。
含有所述Os516基因的表达盒、重组载体或重组微生物均属于本发明的保护范围。
本发明还保护Os516蛋白的应用，为如下：调控植物的株高性状和/或穗长性状。
本发明还保护Os516基因的应用，为如下：培育株高性状和/或穗长性状改变的转基因植物。
本发明还保护抑制Os516蛋白的物质在植物育种的应用，所述育种的目标为如下：培育株高增高和/或穗长增长的植物。所述抑制Os516蛋白为抑制Os516蛋白的活性和/或降低Os516蛋白的丰度。所述降低Os516蛋白的丰度可通过使Os516蛋白不能被表达实现。
本发明还保护抑制编码Os516蛋白的核酸分子的物质在植物育种的应用，所述育种的目标为如下：培育株高增高和/或穗长增长的植物。所述抑制编码Os516蛋白的核酸分子为抑制编码Os516蛋白的核酸分子的活性和/或降低编码Os516蛋白的核酸分子的丰度。降低编码Os516蛋白的核酸分子的丰度可通过使RNA不能被转录实现。降低编码Os516蛋白的核酸分子的丰度可通过基因编辑实现。所述基因编辑具体可为基于Cas9系统的基因编辑。Cas9系统中，sgRNA中的靶序列结合区如序列表的序列7所示。Cas9系统中，sgRNA如序列表的序列6所示。所述基因编辑具体通过在植物中导入重组质粒SG2027实现。
本发明还保护对Os516基因进行基因编辑的物质在植物育种的应用，所述育种的目标为如下：培育株高增高和/或穗长增长的基因编辑植株。所述基因编辑具体可为基于Cas9系统的基因编辑。对Os516基因进行基因编辑的物质具体可为sgRNA和Cas9蛋白。对Os516基因进行基因编辑的物质具体可为编码sgRNA的DNA分子和编码Cas9蛋白的DNA分子。对Os516基因进行基因编辑的物质具体可为具有编码sgRNA的DNA分子的表达载体和具有编码Cas9蛋白的DNA分子的表达载体。对Os516基因进行基因编辑的物质具体可为具有编码sgRNA的DNA分子和编码Cas9蛋白的DNA分子的表达载体。sgRNA中的靶序列结合区如序列表的序列7所示。sgRNA如序列表的序列6所示。编码sgRNA的DNA分子如序列表的序列5所示。对Os516基因进行基因编辑的物质具体为重组质粒SG2027。
本发明还保护一种制备转基因植物的方法，包括如下步骤：在受体植物中导入抑制Os516基因表达的物质，得到转基因植物；与所述受体植物相比，所述转基因植物株高增高和/或穗长增长。所述抑制Os516基因表达的物质可为编码sgRNA的DNA分子和编码Cas9蛋白的DNA分子。所述抑制Os516基因表达的物质具有编码sgRNA的DNA分子的表达载体和具有编码Cas9蛋白的DNA分子的表达载体。所述抑制Os516基因表达的物质可为具有编码sgRNA的DNA分子和编码Cas9蛋白的DNA分子的表达载体。所述抑制Os516基因表达的物质具体可为重组质粒SG2027。sgRNA中的靶序列结合区如序列表的序列7所示。sgRNA如序列表的序列6所示。编码sgRNA的DNA分子如序列表的序列5所示。对Os516基因进行基因编辑的物质具体为重组质粒SG2027。
本发明还保护一种植物育种方法，包括如下步骤：对受体植物中的Os516基因进行基因编辑，得到基因编辑植株；与所述受体植物相比，所述基因编辑植株的株高增高和/或穗长增长。对受体植物中的Os516基因进行基因编辑具体通过Cas9系统实现。Cas9系统中，sgRNA中的靶序列结合区如序列表的序列7所示。Cas9系统中，sgRNA如序列表的序列6所示。对受体植物中的Os516基因进行基因编辑具体通过导入重组质粒SG2027实现。所述基因编辑植株具体可为用“GCACAGCAAATTACGGA”替换基因组DNA中的Os516基因的“GCACAGCAAATTATTCCGGA”，且为纯合型的植株。
以上任一所述株高为抽穗后期株高。
以上任一所述Cas9蛋白为序列表的序列4中第2697-6968位核苷酸编码的蛋白质。
以上任一所述重组质粒SG2027如序列表的序列4所示。
以上任一所述植物为单子叶植物或双子叶植物。以上任一所述植物为禾本科植物。以上任一所述植物为稻属植物。以上任一所述植物为水稻，例如水稻日本晴。
本发明可用于植物的株高/穗长性状改良，对于植物育种特别是水稻育种，具有重大的应用推广价值。
附图说明
图1为重组质粒SG2027的结构示意图。
图2为突变位点及其周边核苷酸的测序结果。
图3为植株的照片。
图4为植株的株高测量结果。
图5为植株的穗的照片。
图6为植株的穗长测量结果。
具体实施方式
以下的实施例便于更好地理解本发明，但并不限定本发明。下述实施例中的实验方法，如无特殊说明，均为常规方法。下述实施例中所用的试验材料，如无特殊说明，均为自常规生化试剂商店购买得到的。以下实施例中的定量试验，均设置三次重复实验，结果取平均值。
实施例
Os516蛋白如序列表的序列1所示。水稻日本晴的cDNA中，编码Os516蛋白的CDS如序列表的序列2所示。水稻日本晴的基因组DNA中，编码Os516蛋白的基因如序列表的序列3所示。
一、构建重组质粒
构建重组质粒SG2027。重组质粒SG2027的结构示意图见图1。经全质粒测序，重组质粒SG2027如序列表的序列4所示。序列表的序列4中，第2697-6968位核苷酸编码Cas9蛋白。重组质粒SG2027中，sgRNA的编码区如序列表的序列5所示。相应的，sgRNA如序列表的序列6所示，sgRNA中的靶序列结合区如序列表的序列7所示。
二、进行遗传转化并获得再生植株
将重组质粒SG2027导入农杆菌EHA105，得到重组农杆菌。采用农杆菌浸染法，将重组农杆菌对水稻日本晴的胚性愈伤组织进行遗传转化，然后筛选抗性愈伤组织(抗性筛选采用100mg/L潮霉素)，然后进行分化再生培养，然后进行生根培养，得到再生植株。
三、获得转基因植株及其后代植株
将步骤二得到的再生植株进行如下鉴定：取叶片，提取基因组DNA，采用引物F和引物R组成的引物对进行PCR扩增，然后将PCR扩增产物进行测序。
F：5’-TAGCGGCATCTCGGTTTTGA-3’；
R：5’-AGGCCAAACATGTGGATGGA-3’。
通过以上鉴定，从步骤二得到的再生植株中筛选得到一个纯合突变型植株(即两条染色体发生的突变一致)，命名为Os516-1植株。经测序鉴定，与水稻日本晴(用Nip表示)的基因组DNA相比，Os516-1植株(用Os516表示)的差异仅在于在编码Os516蛋白的基因中发生了三个核苷酸的缺失(引起三个核苷酸的缺失，具体为“ATTATTCCG”突变为“ATTACG”，相应编码的氨基酸残基由“IIP”突变为“IT”)，该突变位点及其周边核苷酸的测序结果见图2。
Os516-1植株自交并收获种子，将种子培育为植株，即为T1代植株。T1代植株自交并收获种子，即为T2代种子。Os516-1植株及其自交后代，称为Os516-1株系。
四、性状比较
供试种子为：水稻日本晴种子、Os516-1株系的T2代种子。
在平行条件下培养供试植株，具体为：取种子，在温室萌发并育苗(从露白开始计时，共培养3周)，得到3周幼苗；将3周幼苗移栽至河北廊坊的大田并正常栽培管理。
在大田中栽培管理110天后植株(处于抽穗后期)的照片见图3。在大田中栽培管理110天后植株的株高见图4(每个株系取10株植株的平均值)。图3和图4中，Nip表示水稻日本晴，Os516表示Os516-1株系。日本晴植株的平均株高为108cm，Os516-1株系植株的平均株高为140cm。结果表明，与水稻日本晴植株相比，Os516-1株系植株的株高显著增加，增加幅度约为29.6％。
在大田中培养110天后(也就是从移栽开始计时培养110天后)植株的穗的照片见图5。穗长见图6(每个株系取10株植株的穗长的平均值)。图5和图6中，Nip表示水稻日本晴，Os516表示Os516-1株系。水稻日本晴植株的平均穗长为23.6cm，Os516-1株系植株的平均穗长为27.8cm。结果表明，与水稻日本晴植株相比，Os516-1株系植株的穗长显著增加，增加幅度约为17.8％。
SEQUENCE LISTING
<110> 中国农业科学院生物技术研究所
<120> Os516蛋白及其编码基因和应用
<130> GNCYX200529
<160> 7
<170> PatentIn version 3.5
<210> 1
<211> 880
<212> PRT
<213> Oryza sativa
<400> 1
Met Gly Gly Gly Arg Lys Arg Gly Arg Thr Gln Arg Arg His Phe Lys
1 5 10 15
Gln Gly Arg Glu Asn Val Trp Lys His Asn Pro Gln Arg Pro Pro Ala
20 25 30
Ala Gly Gly Glu Gly Ala Glu Gly Gly Ala Ala Glu Gly Arg Glu Gly
35 40 45
Asn Pro Ser Trp Gln Pro Phe Ala Thr Glu Asn Pro Ala Phe Glu Asp
50 55 60
Tyr Tyr Lys Ala Gln Gln Ile Ile Pro Glu Gly Glu Trp Asp Asp Phe
65 70 75 80
Met Asn Met Leu Arg Lys Pro Leu Pro Ala Thr Phe Arg Ile Asn Ala
85 90 95
Ser Cys Gln Phe Tyr Gln Asp Ile Cys Ser Gln Leu Glu Asn Asp Phe
100 105 110
Arg Lys Ser Leu Glu Thr Glu Val Ser Asp Glu His Glu Glu Asp Ala
115 120 125
Ile Arg Pro Leu Pro Trp Tyr Pro Gly Asn Leu Ala Trp His Leu Asn
130 135 140
Phe Ser Arg Met Gln Leu Arg Arg Asn Gln Ala Leu Glu Gly Phe His
145 150 155 160
Glu Phe Leu Lys Arg Glu Asn Glu Val Gly Asn Ile Thr Arg Gln Glu
165 170 175
Ala Val Ser Met Val Pro Pro Leu Phe Leu Asn Val Gln Pro Asp His
180 185 190
His Ile Leu Asp Met Cys Ala Ala Pro Gly Ser Lys Thr Phe Gln Leu
195 200 205
Leu Glu Met Ile His Gln Ser Thr Lys Pro Gly Met Leu Pro Asn Ala
210 215 220
Leu Val Val Ala Asn Asp Val Asp Val Gln Arg Cys Asn Leu Leu Ile
225 230 235 240
His Gln Thr Lys Arg Met Cys Thr Ala Asn Leu Ile Val Thr Asn His
245 250 255
Glu Ala Gln Asn Phe Pro Gly Cys Asn Leu Ala Lys Phe Ser Ser Glu
260 265 270
Thr Cys Thr Asp Glu Ser Lys Leu Gln Arg Leu Glu Phe Asp Arg Val
275 280 285
Leu Cys Asp Val Pro Cys Ser Gly Asp Gly Thr Val Arg Lys Ala Pro
290 295 300
Asp Met Trp Arg Lys Trp Asn Ala Gly Met Gly Asn Gly Leu His Arg
305 310 315 320
Leu Gln Val Glu Ile Ala Met Arg Gly Ile Gly Leu Leu Lys Val Gly
325 330 335
Gly Arg Ile Val Tyr Ser Thr Cys Ser Met Asn Pro Val Glu Asn Glu
340 345 350
Ala Val Val Ala Glu Ile Leu Arg Arg Cys Gly Asp Ser Val Glu Leu
355 360 365
Leu Asp Val Ser Asn Glu Leu Pro Glu Leu Val Arg Arg Pro Gly Leu
370 375 380
Ser Thr Trp Lys Val Arg Asp Arg Gly Ser Trp Phe Gly Thr His Glu
385 390 395 400
Asp Val Pro Arg Tyr Arg Lys Asn Val Ile Ser Pro Ser Met Phe Pro
405 410 415
Ser Gly Lys Gly Thr Met Asp Ser His Val Ala Ile Gly Ser Val Glu
420 425 430
Ile Asn Thr Asp Val Ile Asp Ala Asp Met Lys Asp Ser Thr Asn Met
435 440 445
Val Glu Gly Glu Gln Glu Thr Lys Thr Ala Ser Asp Asp Val Asn Asn
450 455 460
Gly Gly Asp Pro Asn Thr Glu Glu Thr Ser Lys Leu Glu Ser Asn Glu
465 470 475 480
Val Pro Asn Asp Ser Asp Lys Lys Ser Asn Ser Thr Ser Ile Arg Thr
485 490 495
Glu His Ser Asn Phe Pro Leu His Arg Cys Met Arg Ile Val Pro His
500 505 510
Asp Gln Asn Ser Gly Ala Phe Phe Ile Ala Val Leu Gln Lys Ile Ser
515 520 525
Pro Ile Asn Gly Asn Gln Glu Ala Glu Leu Ile Lys Gly Glu His Asn
530 535 540
Ile Ser Lys Asp Arg Ala Glu Lys Leu Glu Lys Gly Leu Gly Ser Asp
545 550 555 560
Lys Val Pro His Lys Glu Asn Thr Val Gln Gln Gln Gly Val Asp Asp
565 570 575
Gly Asn Val Met Asp Glu Gln Gln Asn Gly Asp Val Asp Asn Glu Thr
580 585 590
Ser Asn Gly Lys Ser Ser Glu Glu Ala Lys Val Ile Val Asn Glu Ala
595 600 605
Glu Asn Asp Gln Ala Gly Pro Arg Asp Arg Arg Arg Lys Pro Gln Asn
610 615 620
Gln Gly Arg Trp Arg Gly Val Asp Pro Val Ile Phe Phe Lys Asp Glu
625 630 635 640
Ala Thr Ile Arg Ser Ile Val Ser Phe Tyr Gly Ile Lys Asp Thr Phe
645 650 655
Pro Leu Glu Gly His Leu Val Thr Arg Asn Pro Asp Ala Gly His Val
660 665 670
Lys Arg Ile Tyr Tyr Val Ser Lys Ser Val Gln Glu Val Leu Glu Leu
675 680 685
Asn Val Lys Val Gly Glu Arg Leu Lys Ile Thr Ser Leu Gly Leu Lys
690 695 700
Ile Phe Glu Arg Gln Ser Ser Lys Asp Gly Ser Pro Cys Thr Phe Arg
705 710 715 720
Leu Ser Ser Glu Gly Leu Pro Leu Leu Leu Pro Tyr Ile Thr Lys Gln
725 730 735
Ile Leu Tyr Ala Ser Ala Ile Asp Phe Gln His Leu Leu Gln Tyr Arg
740 745 750
Thr Ile Lys Phe Pro Asp Phe Val Asp Ala Lys Phe Gly Glu Glu Ala
755 760 765
Ser Ala Leu Leu Pro Gly Cys Cys Val Val Val Leu Trp Glu Gly His
770 775 780
Gln Asn Ile Asp Ser Ile Ala Met Asp Pro Ser Ala Ile Ala Ile Val
785 790 795 800
Cys Trp Lys Gly Lys Thr Asn Leu Cys Val Met Val Ser Pro Leu Asp
805 810 815
Gly Lys Glu Leu Leu Glu Arg Ile Cys Leu Arg Tyr Gly Leu Lys Ile
820 825 830
Pro Lys Ala Asp Asp Val Lys Pro Ser Met Lys Ile Asp Gly Ser Asp
835 840 845
Glu Gln Pro Asp Leu Ser Thr Glu Ala Val Asp Pro Glu Ala Val Pro
850 855 860
Glu Ser Lys Ala Ser Asp Met Glu Ile Ala Asp Ala Lys Glu Val Glu
865 870 875 880
<210> 2
<211> 2643
<212> DNA
<213> Oryza sativa
<400> 2
atgggaggcg gcaggaagcg cgggcgcacg cagcgccgcc acttcaagca ggggcgggag 60
aacgtctgga agcacaaccc gcagcgcccc ccggcggctg gcggcgaagg agccgaagga 120
ggcgctgccg aggggcgcga agggaacccc tcgtggcagc ccttcgccac cgagaaccca 180
gccttcgagg actactacaa ggcacagcaa attattccgg agggagagtg ggacgacttc 240
atgaacatgc tccggaaacc gctgccagcc actttcagga ttaatgcgag ctgtcaattt 300
tatcaagata tttgctcaca gttagaaaat gacttcagga agtcattgga aactgaggtt 360
agtgatgagc atgaagaaga tgctattcgg cctttgcctt ggtaccctgg caatcttgca 420
tggcatttga atttttctcg aatgcagctg aggagaaacc aggcacttga gggttttcat 480
gaattcttga agcgagagaa tgaagttggc aatataacta ggcaagaggc tgtcagcatg 540
gttcctcctt tgtttctgaa tgtgcaacct gatcatcata ttcttgacat gtgcgctgct 600
ccaggatcaa agaccttcca gttacttgag atgatccatc agtctacaaa gcctggaatg 660
cttccaaatg cccttgtggt agctaatgat gttgatgtgc aaagatgcaa tcttcttatt 720
catcagacaa agagaatgtg cacagccaac ttgattgtga cgaatcatga agcacagaac 780
tttcctggct gtaatcttgc aaagtttagt tcagaaacat gcacggatga gtccaaactg 840
cagaggttgg aatttgatcg tgtattgtgt gatgtgcctt gtagtggtga tggaactgtg 900
cgtaaagctc ctgatatgtg gagaaagtgg aatgctggta tggggaatgg actccatcgt 960
ctccaagtag aaattgcaat gcgcggtatt ggtttgctta aagtgggtgg aaggattgtt 1020
tactcaacat gttcaatgaa tcctgttgaa aatgaagcag ttgtcgcgga gattctacgg 1080
agatgtgggg attctgttga acttcttgat gtttctaatg agctacctga attagtcagg 1140
cgtcctggac ttagcacctg gaaggtacgg gatagagggt cttggtttgg cactcacgaa 1200
gatgtccctc gctacagaaa gaacgtgata tcaccaagca tgtttccttc agggaagggc 1260
accatggata gccatgtggc tattggcagt gttgagatca acacagatgt aattgatgct 1320
gatatgaaag attcaacaaa catggtagag ggagaacaag aaacgaaaac agcatctgat 1380
gatgtcaata atggtggtga tcccaatact gaagagacga gcaaacttga gtctaatgaa 1440
gttccaaacg actctgataa aaagtcaaat tctacgtcta tccgcacaga acattcaaat 1500
ttccctctgc atcgctgcat gagaattgtt ccgcatgatc aaaacagtgg ggcatttttt 1560
attgcagtcc ttcagaaaat ctcccctatt aatgggaacc aagaggcaga acttataaaa 1620
ggcgagcaca atatttcaaa ggatagggct gagaaacttg agaaaggtct cggatcagat 1680
aaggtgccac ataaagaaaa taccgtgcag cagcaaggag ttgatgatgg taatgttatg 1740
gatgaacagc aaaatggaga tgtggataat gaaacatcaa atggtaaaag ctcagaggaa 1800
gctaaagtaa ttgttaatga ggcagaaaat gatcaagcag gaccaagaga taggaggagg 1860
aagccacaga accaaggaag gtggagaggg gttgatccag tgatattttt caaagatgaa 1920
gctacaataa gaagtatagt atctttctat ggtatcaagg atacatttcc gcttgagggt 1980
caccttgtga ctaggaatcc tgatgctggc catgttaaaa gaatatacta cgtgtcaaaa 2040
tcagtgcaag aagttttgga gctcaatgta aaagttggtg agcggcttaa gattacctca 2100
cttggcctaa agatatttga aagacagtca tcaaaggatg gctcaccatg cacatttagg 2160
ttgtcttcag agggtttacc actgctgctt ccatacatca ccaaacagat tctctacgct 2220
tctgcaattg acttccagca ccttttacaa tacagaacta ttaaatttcc tgattttgtg 2280
gatgcaaaat tcggtgaaga agcttcagct ttgttgcctg gttgctgtgt tgtagtactt 2340
tgggaagggc atcagaacat agattccatc gccatggatc cttctgcaat cgccattgtt 2400
tgctggaaag ggaagaccaa tttgtgcgtc atggtttctc ccctggatgg gaaggagctg 2460
cttgagagga tttgtttacg ttatgggctc aaaatcccca aagcggatga tgtaaaaccc 2520
agcatgaaga ttgatggatc agacgagcag cctgatctca gcactgaggc agttgatcca 2580
gaagctgtgc ccgaaagcaa agcatctgac atggagattg cagatgctaa agaggtggag 2640
taa 2643
<210> 3
<211> 7408
<212> DNA
<213> Oryza sativa
<400> 3
cctccccacc ccgcatcact cgccgccgct cctaaacccc tcgaagagaa ccccctcctc 60
ccctcgcgcc accgccgccc gcatccgcgc cgtcaccggg agggagagca gcagcggcgg 120
aagcaggagc agctcgcgcg agagagggag gaagccatgg gaggcggcag gaagcgcggg 180
cgcacgcagc gccgccactt caagcagggg cgggagaacg tctggaagca caacccgcag 240
cgccccccgg cggctggcgg cgaaggagcc gaaggaggcg ctgccgaggg gcgcgaaggg 300
aacccctcgt ggcagccctt cgccaccgag aacccagcct tcgaggacta ctacaaggtg 360
cggttgctta atattttact ttttttgggt gtgatttggt tgtgttagta aaaaaattga 420
gctgagctag cggcatctcg gttttgatcg tatggtctcg ctctaggtat gtgtacttcg 480
tttagaatgt gatgatttgt tgtcttgttg attgcggata ttatagcttt caagcaagaa 540
gcaactgctc tattttgatt gtgatgctga gtagcgagta ctgatctcta gacttacctt 600
acgataagag aagcctaact ggacgcactg cattagctta tactgttgtt gcttattagc 660
ttagtttttc agttggatgc ataacttctg aattagaatg tttatacatg tttatggctt 720
ataggcacag caaattattc cggagggaga gtgggacgac ttcatgaaca tgctccggaa 780
accgctgcca gccactttca ggattaatgc gaggtaatgt tgttttcatc tgaagcaaca 840
tgactccatc cacatgtttg gcctaatgat ttattgtttg ttggtcacat tgtattgaat 900
ggcagtgcag ttagcttgtt tatgacacta ccttgattat tgcaatatag cagcatcaca 960
ttttaaagaa aatcattcct tgagttctga tgattgtgta atgtagcttt tcatgaccaa 1020
acagcttgcg atattgctcg aaatcaccaa gaaggcaaaa attccttggt ggtgttgtat 1080
atgttctggg tgaaatatct ataagctctc aaaactttgt tccaacaaaa atgaatctgg 1140
gggattatcc tagcttataa aaagatatag atatagattt tatactgttt tctatacaat 1200
caaaactttt aatcttatct tgctaactct ctattgctgt tatttaaact ctaatcatat 1260
ataggttcta gatatctgcc tattgtttga attgataaaa accatgctct gtttgaataa 1320
tcttttactt gactccatat aactttcact gtccttttgt aaaagaaaag gacattatct 1380
ctatcctgat tctcctgttt tttccctgcc atggttcatt cttcagatat aaccatctcc 1440
agttctatgt gcaattgatc atatagttag aacaaaagca tttgcatgaa atgcactggt 1500
gattttactg ataccttatc atgtctggta tcattgcaca taactaattc ttgttttatg 1560
atgattattc tgttctagta tgctaatgtt gtccttacct ttccagctgt caattttatc 1620
aagatatttg ctcacagtta gaaaatgact tcaggaagtc attggaaact gaggtagctt 1680
ctatattttg tgcataaatt ttagttatgt ccttcacaat tctattttgt aatccttttt 1740
tttttttttg catggacagg ttagtgatga gcatgaagaa gatgctattc ggcctttgcc 1800
ttggtaccct ggcaatcttg catggcattt gaatttttct cgaatgcagc tgaggagaaa 1860
ccaggcactt gaggggtatg cataatgttt atcttgggag aattgtgtca acaatgcgca 1920
gtatatagga tgattgtttt ttaggagtgt ggtaaactga tgtttttgtt acaataaaac 1980
tcccttcacc tggtttttgt catctcatgt atataccatc attactaaat atcaccatag 2040
cgttagcacg ggcacattac tagtttgtgt aaattgatga atttattgag aacatggttg 2100
ccccttaaat gatgaattac taaccaattt atggctttat agatcagtga ttgccttgaa 2160
ttctctacta aaccatcacc aaaagttcac caaaacttct caaatatttg tgtttggctt 2220
tttgttgcag atttagtgtt atatcactgt tagcagtttt tcataagatt ttttagggct 2280
agtttctttt gaaaaagaaa ctgaaaaaaa ttgcacttaa caattgctgc cattgaaaca 2340
ttggaggcgc tcatttaata ttctattgag acataatact tttttgtaat atttgtttcc 2400
ctctccagtt ttcatgaatt cttgaagcga gagaatgaag ttggcaatat aactaggcaa 2460
gaggctgtca gcatggtaag ttccttatat atattaagat gagctatatg agtatattgt 2520
ttattgatga tggtctatgt atattgcgga tattcttctt gttaaaatga tatgtaatat 2580
ttttacaggt tcctcctttg tttctgaatg tgcaacctga tcatcatatt cttgacagta 2640
tgtatatcca cgttcttttc catcattcct catattgtgc tatttatgca gctggctgtc 2700
tgtctcttga cctgcatgtt tgacctaact ttgctcttct gtagtccagc ttgtaatgtt 2760
taaaactaat tgctcaataa agcatctggc gcaatagttc ctaataaaag tttataagtc 2820
tcaggaacat gttaatttat tagtaccagt aaaaaggaaa aaaatgagaa caccagaata 2880
ccccgtagtt atcatttaga cctagccttt tctgtttggc tttagcttta tatcagactt 2940
agcttttgag gcatttgagc ttaaaagtag gttacacatg gactatttga aaattttatt 3000
tttagaggag gacttctcca atattggtct tgtgaatttt tgtggtatta tcctatgcag 3060
tgtgcgctgc tccaggatca aagaccttcc agttacttga gatgatccat cagtctacaa 3120
agcctggaat gcttccaaat gcccttgtat gtttcacatt ctattacaca tatctatagt 3180
ttctggtaac gatagaaaaa aaaactttga atcaaaacct tttttctgat tttgtgtttc 3240
atactttgta ctcaggtggt agctaatgat gttgatgtgc aaagatgcaa tcttcttatt 3300
catcagacaa agagaatgtg cacagccaac ttgattgtga cgaatcatga agcacagaac 3360
tttcctggct gtaatcttgc aaagtttagt tcagaaacat gcacggatga gtccaaactg 3420
cagaggttgg aatttgatcg tgtattgtgt gatgtgcctt gtagtggtga tggaactgtg 3480
cgtaaagctc ctgatatgtg gagaaagtgg taagtagtga aattttttag ccgtggtgct 3540
actgtcatcg tcttccctat ttccttatca attttatttg ccactcagga atgctggtat 3600
ggggaatgga ctccatcgtc tccaagtaga aattgcaatg cgcggtaatg atttgaagag 3660
gctaaaacta tcatatgttc caatgaactg ttaacttttt tcgctatgtt ggacttttgt 3720
ttcttgaaat gtaaatgtaa ctaaccattg taaagcaatt tttatgtagt tttctcctcc 3780
ttagacttgt gattttatca attcactatc tattattata ttccgtattt tactatgaac 3840
caggtccttg tagctgtggt gtgtcattaa atgttccatg aactgagatt gataaaatag 3900
ctaactggtt agtcttataa gttcatttac atacatgata gcaagatatg tgcatctggt 3960
atggattcct tttcaattac cgatcatgtg taatcaagta ttaaatcagc acaaatttga 4020
tactttgtaa tgtgtaacat gatacgttag ttgctaattg ccatcaatgc tatgctacct 4080
caagagatta agattctttg ttttattgtt caggtattgg tttgcttaaa gtgggtggaa 4140
ggattgttta ctcaacatgt tcaatgaatc ctgttgaaaa tgaagcagtt gtcgcggagg 4200
tattttttgt tgcttgattt aactttactt cctttgcccc tctctaatat taatgtagta 4260
atgtttgatt gatcaaatta tttttgttgc ctttttcttt gtcatacact tatacttgat 4320
gagaccctaa aatgttttca gtgtgtcaaa tctaccatgg gagtaacttt tgttgagatt 4380
agaccatcta ggaatgttca tttttcaagt gtagcacttg ttttcatgtc ctgtgtacct 4440
tgttgtgaca ttgaccatat ttttcataac taaatagatt ctacggagat gtggggattc 4500
tgttgaactt cttgatgttt ctaatgagct acctgaatta gtcaggcgtc ctggacttag 4560
cacctggaag gtcagtgtac atttctctga atcatgcata tttccttgta tattttgtat 4620
cactttgcaa ttttgcataa ttagctgcga ctgtgatctt ctgtggcctt agagccaaaa 4680
aagaaaaaaa aaagtcaccc taggttcacc caatctgctg taatgtgctg aactgcgatt 4740
actacatgtt ttaattatca atatcatgga cacatggttc atcttccatt tatatggtct 4800
agattagatc tgtctgagtt tttcactgga taaagtggtg actgagtttg catttacatg 4860
tagcaataca caactatatc ttgaacatct ttttatttac tgttttgatc aatgttgcta 4920
ctttgtttgt tttagtagtt tcttagatgt gatatgaaga gtagtggatg ggtgtccaat 4980
tttgtaaata ttctactgtg cttttcagtt tatgaaactt gtgcaaggac aatggaggta 5040
gcagccattg ttttagtttt tttggagtta tttcttagtc agagttgttt gcattctgct 5100
ctgttggttt tgttattctg tcacagaagt attctccgtt ggggggcccc tctaaatttg 5160
atatcccaca ctctggagta tttgattctt ttgcagttat tgccttccta actgttgcag 5220
gtacgggata gagggtcttg gtttggcact cacgaagatg tccctcgcta cagaaagaac 5280
gtgatatcac caagcatgtt tccttcaggg aagggcacca tggatagcca tgtggctatt 5340
ggcagtgttg agatcaacac agatgtaatt gatgctgata tgaaagattc aacaaacatg 5400
gtagagggag aacaagaaac gaaaacagca tctgatgatg tcaataatgg tggtgatccc 5460
aatactgaag agacgagcaa acttgagtct aatgaagttc caaacgactc tgataaaaag 5520
tcaaattcta cgtctatccg cacagaacat tcaaatttcc ctctgcatcg ctgcatgaga 5580
attgttccgc atgatcaaaa cagtggggca ttttttattg cagtccttca gaaaatctcc 5640
cctattaatg gtaatcttta ggaatttatt ccatgtattt catgacttgt atcaaattgc 5700
ataaacttat tagccactgc ttattaatgt tcaagtgtgc ataccttaat atctttggtt 5760
ctttgcaggg aaccaagagg cagaacttat aaaaggcgag cacaatattt caaaggatag 5820
ggctgagaaa cttgagaaag gtctcggatc agataaggtg ccacataaag aaaataccgt 5880
gcagcagcaa ggagttgatg atggtaatgt tatggatgaa cagcaaaatg gagatgtgga 5940
taatgaaaca tcaaatggta aaagctcaga ggaagctaaa gtaattgtta atgaggcaga 6000
aaatgatcaa gcaggaccaa gagataggag gaggaagcca cagaaccaag gaaggtggag 6060
aggggttgat ccagtgatat ttttcaaaga tgaagctaca ataagaagta tagtatcttt 6120
ctatggtatc aaggatacat ttccgcttga gggtcacctt gtgactagga atcctgatgc 6180
tggccatgtt aaaagaatat actacgtgtc aaaatcagtg caagaagttt tggagctcaa 6240
tgtaaaagtt ggtgagcggc ttaagattac ctcacttggc ctaaagatat ttgtaagtgt 6300
ctccttaatt tatgttttct ttatcaattt tagcttttat cagtggggca aatggcacga 6360
cactaagctg tgattcttgc aatatctttc aggaaagaca gtcatcaaag gatggctcac 6420
catgcacatt taggttgtct tcagagggtt taccactgct gcttccatac atcaccaaac 6480
agattctcta cgcttctgca attgacttcc agcacctttt acaatacaga actattaaat 6540
ttcctgattt tgtggatgca aaattcggtg aagaagcttc agctttgttg cctggttgct 6600
gtgttgtagt actttgggaa ggtaacatca catcccactt ataagcttta tagagaaaca 6660
ccacaacttg atgacagcta aacatgtttg aaatatttca gggcatcaga acatagattc 6720
catcgccatg gatccttctg caatcgccat tgtttgctgg aaagggaaga ccaatttgtg 6780
cgtcatggtt tctcccctgg atgggaagga gctgcttgag aggatttgtt tacgttatgg 6840
gctcaaaatc cccaaagcgg atgatgtaaa acccagcatg aagattgatg gatcagacga 6900
gcagcctgat ctcagcactg aggcagttga tccagaagct gtgcccgaaa gcaaagcatc 6960
tgacatggag attgcagatg ctaaagaggt ggagtaatcc tgctgaatgc tgatgctatc 7020
caaggccaca tattatagct ggatacaaca tcgccccatc atttgcctac cggtattagt 7080
tgagcgatga gccgccattt ttcattgatt ctggcattta cgccgttttc ctgtttgatt 7140
tatcatattc tgtttctgaa gaatgggtga atgaactcgc ccattagtga aagggcacca 7200
cgttgctcta aagcccatct gtccatgtca tctggtaatc gtgcacgtat tgccccctcc 7260
atacttccgg ctggctcatc cacctgtaac ctatcagtat cagcttattt ttcttcgaag 7320
agaatgtatt acagggagtc ttaacctgaa ctcttgaacc tgataaattt tgatatgaca 7380
atatttcaga cacatcctct tgactaaa 7408
<210> 4
<211> 15898
<212> DNA
<213> Artificial sequence
<400> 4
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttggatcat gaaccaacgg cctggctgta 300
tttggtggtt gtgtagggag atggggagaa gaaaagcccg attctcttcg ctgtgatggg 360
ctggatgcat gcgggggagc gggaggccca agtacgtgca cggtgagcgg cccacagggc 420
gagtgtgagc gcgagaggcg ggaggaacag tttagtacca cattgcccag ctaactcgaa 480
cgcgaccaac ttataaaccc gcgcgctgtc gcttgtgtgc acagcaaatt attccggagt 540
tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg 600
caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc 660
cgtagcgcgt gcgccaattc tgcagacaaa tggccccggg cctgcaggtg cagcgtgacc 720
cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca 780
catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta 840
aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa 900
tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac 960
tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac 1020
ctatataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt 1080
ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa 1140
actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa 1200
agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt 1260
cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca 1320
accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc 1380
gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc 1440
atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct 1500
cctctcacgg cacggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 1560
ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 1620
tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 1680
caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc 1740
ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt 1800
gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc 1860
tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg 1920
cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt 1980
tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt 2040
tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct 2100
gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt 2160
catagttacg aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg 2220
atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt 2280
ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac 2340
ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag 2400
tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg 2460
atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat 2520
ctattataat aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg 2580
catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct 2640
tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcagccatgg 2700
actataagga ccacgacgga gactacaagg atcatgatat tgattacaaa gacgatgacg 2760
ataagatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca 2820
agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg 2880
acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca 2940
tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc 3000
ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc 3060
aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag 3120
agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg 3180
tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg 3240
tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca 3300
agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca 3360
agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg 3420
ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg 3480
aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg 3540
ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca 3600
aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg 3660
gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga 3720
gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca 3780
agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc 3840
tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca 3900
ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga 3960
tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc 4020
ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc 4080
tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga 4140
tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg 4200
cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg 4260
acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc 4320
ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg 4380
agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg 4440
agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc 4500
agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg 4560
tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg 4620
acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc 4680
tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt 4740
tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga 4800
gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc 4860
tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga 4920
cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc 4980
acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg 5040
tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa 5100
tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc 5160
ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa 5220
acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt 5280
acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc 5340
ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga 5400
accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact 5460
ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg 5520
ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg 5580
aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt 5640
acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg 5700
tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc 5760
acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta 5820
agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg 5880
ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca 5940
tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga 6000
tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg 6060
tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag 6120
gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa 6180
agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg 6240
tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc 6300
tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg 6360
aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc 6420
tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg 6480
gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg 6540
agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca 6600
agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg 6660
ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca 6720
gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg 6780
ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc 6840
tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt 6900
ctcagctggg aggcgacaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga 6960
aaaagtaagg atcctgattg atcgatagag ctcgaatttc cccgatcgtt caaacatttg 7020
gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt 7080
tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag 7140
atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat 7200
atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga 7260
attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 7320
acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 7380
tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7440
tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct 7500
tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc 7560
agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg 7620
attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac 7680
ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag 7740
tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac 7800
cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca ctctcgtcta 7860
ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca 7920
aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa 7980
aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc 8040
tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag 8100
catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat 8160
ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc ttcctctata 8220
taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat 8280
ctatctctct cgagctttcg cagatcccgg ggggcaatga gatatgaaaa agcctgaact 8340
caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat 8400
gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata 8460
tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca 8520
ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt ttagcgagag 8580
cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac 8640
cgaactgccc gctgttctac aaccggtcgc ggaggctatg gatgcgatcg ctgcggccga 8700
tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac 8760
atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat 8820
ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga 8880
ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac 8940
ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca 9000
atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac 9060
gcgctacttc gagcggaggc atccggagct tgcaggatcg ccacgactcc gggcgtatat 9120
gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc 9180
agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg 9240
tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc 9300
cgatagtgga aaccgacgcc ccagcactcg tccgagggca aagaaataga gtagatgccg 9360
accggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag 9420
ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt 9480
agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa 9540
aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa 9600
acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc 9660
tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata 9720
caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt 9780
tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg 9840
gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga 9900
tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa 9960
ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga 10020
gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg 10080
aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac 10140
cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa 10200
aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg 10260
gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg 10320
cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt 10380
tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag 10440
tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac 10500
agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa 10560
aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg 10620
gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt 10680
gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag 10740
tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat 10800
gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat 10860
attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag 10920
ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 10980
ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 11040
tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 11100
cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 11160
gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 11220
gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 11280
tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 11340
ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 11400
gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 11460
ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 11520
tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 11580
ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 11640
gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 11700
acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt 11760
ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc 11820
tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg 11880
ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 11940
catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 12000
cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc 12060
gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag 12120
cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga 12180
agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc 12240
aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt 12300
ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc 12360
ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc 12420
acaggaaaga gtccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc 12480
tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact 12540
aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac 12600
ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt 12660
tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg 12720
cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc 12780
acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg 12840
atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca 12900
ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg 12960
tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg 13020
gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg 13080
tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt 13140
accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc 13200
ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg 13260
aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga 13320
acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct 13380
gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg 13440
gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc 13500
accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca 13560
cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc 13620
gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc 13680
tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta 13740
ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg 13800
tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa 13860
agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc 13920
tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac 13980
ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc 14040
agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta 14100
gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc 14160
ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg 14220
tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc 14280
ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc 14340
ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc 14400
gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg 14460
atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag 14520
aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc 14580
tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt 14640
ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat 14700
acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag 14760
gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg 14820
tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta 14880
atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt 14940
cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc 15000
gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg 15060
agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca 15120
gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc 15180
ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga 15240
actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg 15300
cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt 15360
ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc 15420
cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga 15480
tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca 15540
agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt 15600
tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg 15660
cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt 15720
gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc 15780
agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac 15840
atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccgt tattctaa 15898
<210> 5
<211> 96
<212> DNA
<213> Artificial sequence
<400> 5
gcacagcaaa ttattccgga gttttagagc tagaaatagc aagttaaaat aaggctagtc 60
cgttatcaac ttgaaaaagt ggcaccgagt cggtgc 96
<210> 6
<211> 96
<212> RNA
<213> Artificial sequence
<400> 6
gcacagcaaa uuauuccgga guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugc 96
<210> 7
<211> 20
<212> RNA
<213> Artificial sequence
<400> 7
gcacagcaaa uuauuccgga 20
Claims (5)
1.Os516蛋白的应用，为如下：调控植物的株高性状和/或穗长性状；
Os516蛋白为如下（a1）或（a2）：
（a1）序列表中序列1所示的蛋白质；
（a2）在（a1）所述蛋白质的N端或/和C端连接标签得到的融合蛋白；
所述应用为通过抑制所述Os516蛋白的表达使得植物株高增高和/或穗长增长；
所述植物为水稻。
2.编码Os516蛋白的核酸分子的应用，为如下：培育株高性状和/或穗长性状改变的转基因植物；
Os516蛋白为如下（a1）或（a2）：
（a1）序列表中序列1所示的蛋白质；
（a2）在（a1）所述蛋白质的N端或/和C端连接标签得到的融合蛋白；
所述应用为通过抑制编码Os516蛋白的核酸分子表达使得植物株高增高和/或穗长增长；
所述植物为水稻。
3.如权利要求2所述的应用，其特征在于：
编码Os516蛋白的核酸分子为如下（b1）或（b2）：
（b1）编码区如序列表中序列2所示的DNA分子；
（b2）序列表中序列3所示的DNA分子。
4.一种制备转基因植物的方法，包括如下步骤：在受体植物中抑制编码Os516蛋白的核酸分子的表达，得到转基因植物；与所述受体植物相比，所述转基因植物的株高增高和/或穗长增长；
Os516蛋白为如下（a1）或（a2）：
（a1）序列表中序列1所示的蛋白质；
（a2）在（a1）所述蛋白质的N端或/和C端连接标签得到的融合蛋白；
所述植物为水稻；
所述抑制编码Os516蛋白的核酸分子的表达为：通过基于Cas9系统的基因编辑降低编码Os516蛋白的核酸分子的丰度。
5.如权利要求4所述的方法，其特征在于：
编码Os516蛋白的核酸分子为如下（b1）或（b2）：
（b1）编码区如序列表中序列2所示的DNA分子；
（b2）序列表中序列3所示的DNA分子。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010142560.6A CN111217897B (zh) | 2020-03-04 | 2020-03-04 | Os516蛋白及其编码基因和应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010142560.6A CN111217897B (zh) | 2020-03-04 | 2020-03-04 | Os516蛋白及其编码基因和应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111217897A CN111217897A (zh) | 2020-06-02 |
| CN111217897B true CN111217897B (zh) | 2021-06-25 |
Family
ID=70811611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010142560.6A Active CN111217897B (zh) | 2020-03-04 | 2020-03-04 | Os516蛋白及其编码基因和应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111217897B (zh) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110092821A (zh) * | 2019-05-30 | 2019-08-06 | 中国农业科学院生物技术研究所 | OsABCB1蛋白及其编码基因和应用 |
| CN110183523A (zh) * | 2019-05-30 | 2019-08-30 | 中国农业科学院生物技术研究所 | OsMYB36蛋白及其编码基因和应用 |
-
2020
- 2020-03-04 CN CN202010142560.6A patent/CN111217897B/zh active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110092821A (zh) * | 2019-05-30 | 2019-08-06 | 中国农业科学院生物技术研究所 | OsABCB1蛋白及其编码基因和应用 |
| CN110183523A (zh) * | 2019-05-30 | 2019-08-30 | 中国农业科学院生物技术研究所 | OsMYB36蛋白及其编码基因和应用 |
Non-Patent Citations (7)
| Title |
|---|
| "GenBank: EEC84738.1, hypothetical protein OsI_ 31727 [Oryza sativa Indica Group];Yu,J.等;《GenBank: EEC84738.1》;20150323;FEATURES和ORIGIN部分 * |
| 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana;Xuean Cui等;《Molecular Plant》;20171130;第10卷;全文 * |
| Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana;Takeshi Itoh等;《Genome Research》;20071230;第17卷;全文 * |
| NCBI Reference Sequence: XM_015756914.2, PREDICTED: Oryza sativa Japonica Group tRNA (cytosine(34)-C(5))-methyltransferase (LOC4347343), mRNA;Unknown;《NCBI Reference Sequence: XM_015756914.2》;20180807;FEATURES和ORIGIN部分 * |
| Plant cytosine-5 DNA methyltransferases: Structure, function and molecular evolution;Athanasia Pavlopoulou等;《Genomics》;20070803;第90卷;全文 * |
| Unknown.NCBI Reference Sequence: XM_015756914.2, PREDICTED: Oryza sativa Japonica Group tRNA (cytosine(34)-C(5))-methyltransferase (LOC4347343), mRNA.《NCBI Reference Sequence: XM_015756914.2》.2018,FEATURES和ORIGIN部分. * |
| Yu,J.等."GenBank: EEC84738.1, hypothetical protein OsI_ 31727 [Oryza sativa Indica Group].《GenBank: EEC84738.1》.2015,FEATURES和ORIGIN部分. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111217897A (zh) | 2020-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110183523B (zh) | OsMYB36蛋白及其编码基因和应用 | |
| AU2016380351A1 (en) | Novel CRISPR-associated transposases and uses thereof | |
| CN113684169B (zh) | 聚(3-羟基丁酸-4-羟基丁酸-5-羟基戊酸)三聚物及其微生物生产菌株构建 | |
| CN113980964B (zh) | 一种甘蓝型油菜BnHBBD基因定点突变的方法及应用 | |
| CA2730126C (en) | Plant regulatory sequence | |
| CN101310020A (zh) | 用于在植物中遗传控制昆虫侵袭的方法和组合物 | |
| CN113481136B (zh) | 重组嗜盐单胞菌及构建方法及催化柠檬酸制备衣康酸的应用 | |
| CN110066829B (zh) | 一种CRISPR/Cas9基因编辑系统及其应用 | |
| CN110092821B (zh) | OsABCB1蛋白及其编码基因和应用 | |
| CN110714022B (zh) | 对花粉竞争力基因stk1；2的表达调控及在提高扩繁植物核雄性不育系效率中的应用 | |
| CN114846022A (zh) | 通过阻抑基因的下调增强作物的疾病抗性 | |
| CN113912686B (zh) | OsRBP2蛋白及其编码基因和应用 | |
| CN109402151A (zh) | 大麦基因HvHVP10及其在提高植物耐盐性方面的应用 | |
| WO2020169221A1 (en) | Production of plant-based active substances (e.g. cannabinoids) by recombinant microorganisms | |
| CN111217897B (zh) | Os516蛋白及其编码基因和应用 | |
| CN114560921B (zh) | OsR5BP1蛋白及其编码基因对植物的耐逆性以及生长性能以及生产性能的调控作用 | |
| KR20220154786A (ko) | 식물의 수율 특성을 개선하기 위한 폴리뉴클레오타이드 분자의 국소 적용 | |
| CN111534578A (zh) | 一种高通量筛选真核生物细胞与农药互作的靶点基因的方法 | |
| CN111549053B (zh) | 一种花椰菜单核苷酸突变的方法 | |
| KR101831121B1 (ko) | 피리피로펜 생합성 유전자 클러스터 및 표지 유전자를 포함하는 핵산 구성체 | |
| CN111793639B (zh) | 一种利用与RNAi工程菌混配提高Bt杀虫活性的方法 | |
| CN112195190B (zh) | 来源于贝莱斯芽孢杆菌质粒的复制元件及其应用 | |
| CN113943741B (zh) | 水稻品质性状基因的基因编辑及应用 | |
| CN114836429B (zh) | PhCLV3和PhCLV1基因在调控矮牵牛植株观赏性中的应用 | |
| CN114573671B (zh) | 闭花授粉性状调控基因BnaC03.FBA、花器官特异性表达启动子PFBA及其应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |