DK2279263T3 - Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer - Google Patents

Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer

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Publication number
DK2279263T3
DK2279263T3 DK09739895.2T DK09739895T DK2279263T3 DK 2279263 T3 DK2279263 T3 DK 2279263T3 DK 09739895 T DK09739895 T DK 09739895T DK 2279263 T3 DK2279263 T3 DK 2279263T3
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Denmark
Prior art keywords
rnase
based assays
modified rna
rna monomers
monomers
Prior art date
Application number
DK09739895.2T
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Danish (da)
English (en)
Inventor
Joseph Alan Walder
Mark Aaron Behlke
Scott Rose
Joseph Dobosy
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Integrated Dna Tech Inc
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Publication of DK2279263T3 publication Critical patent/DK2279263T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Saccharide Compounds (AREA)
DK09739895.2T 2008-04-30 2009-04-30 Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer DK2279263T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4920408P 2008-04-30 2008-04-30
PCT/US2009/042454 WO2009135093A2 (en) 2008-04-30 2009-04-30 Rnase-h-based assays utilizing modified rna monomers

Publications (1)

Publication Number Publication Date
DK2279263T3 true DK2279263T3 (da) 2013-11-11

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK13173388.3T DK2644707T3 (en) 2008-04-30 2009-04-30 RNase-H-based assays using modified RNA monomers.
DK09739895.2T DK2279263T3 (da) 2008-04-30 2009-04-30 Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK13173388.3T DK2644707T3 (en) 2008-04-30 2009-04-30 RNase-H-based assays using modified RNA monomers.

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US (4) US20090325169A1 (enExample)
EP (6) EP2644709B1 (enExample)
JP (6) JP5539325B2 (enExample)
AU (1) AU2009242546B2 (enExample)
CA (1) CA2722541C (enExample)
DK (2) DK2644707T3 (enExample)
WO (1) WO2009135093A2 (enExample)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644709B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
EP2411543B1 (en) 2009-03-27 2017-04-19 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP2013507910A (ja) * 2009-10-13 2013-03-07 シンテッツァ モレキュラー ディテクション イスラエル リミテッド 核酸試料から標的配列を増幅するための方法および組成物
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
JP2011188855A (ja) * 2010-03-11 2011-09-29 Samsung Techwin Co Ltd 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
SG185776A1 (en) 2010-06-14 2013-01-30 Univ Singapore Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr
WO2012021493A2 (en) 2010-08-13 2012-02-16 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
US20120052482A1 (en) * 2010-08-27 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting hepatitis c virus and method of detecting hepatitis c virus using the same
US20120052495A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof
US20120052500A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting chlamydia trachomatis strains and method for detecting chlamydia trachomatis strains using the same
US20120052501A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting htlv strains and use thereof
US20120052503A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting neisseria gonorrhoeae strains and method for detecting neisseria gonorrhoeae strains using the same
US20120052492A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria spp. and use thereof
US20120088246A1 (en) * 2010-10-07 2012-04-12 Samsung Techwin Co., Ltd. Real time pcr detection of single nucleotide polymorphisms
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US20120214160A1 (en) * 2011-01-14 2012-08-23 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012135053A2 (en) * 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2012174484A2 (en) * 2011-06-15 2012-12-20 Nse Products, Inc. Identifying markers of caloric restriction and caloric restriction mimetics
EP2751567B1 (en) * 2011-09-01 2019-05-08 University of Iowa Research Foundation Oligonucleotide-based probes for detection of bacterial nucleases
WO2013123238A1 (en) 2012-02-14 2013-08-22 Great Basin Scientific Methods of isothermal amplification using blocked primers
EP2836609B2 (en) 2012-04-09 2022-06-15 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2013166466A1 (en) * 2012-05-04 2013-11-07 Bio-Rad Laboratories, Inc. Hot-start digital pcr
PT2855692T (pt) 2012-05-24 2019-10-25 Univ Utah Res Found Pcr extrema.
EP3428290B1 (en) * 2012-07-26 2022-04-06 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2943590A4 (en) 2013-01-13 2017-02-15 Unitaq Bio Methods and compositions for pcr using blocked and universal primers
EP2961386B1 (en) 2013-02-28 2019-07-10 The General Hospital Corporation Mirna profiling compositions and methods of use
AU2013382098B2 (en) 2013-03-13 2019-02-07 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
SG11201507512QA (en) * 2013-03-15 2015-10-29 Integrated Dna Tech Inc Rnase h-based assays utilizing modified rna monomers
US9347095B2 (en) 2013-03-15 2016-05-24 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
AU2014348323B2 (en) * 2013-11-14 2020-09-24 Integrated Dna Technologies, Inc. DNA polymerase mutants having enhanced template discrimination activity
WO2015085183A2 (en) * 2013-12-06 2015-06-11 Swift Biosciences, Inc. Cleavable competitor polynucleotides
ES2890078T3 (es) 2013-12-20 2022-01-17 Illumina Inc Conservación de la información de conectividad genómica en muestras de ADN genómico fragmentado
CA2939124C (en) 2014-02-07 2023-08-29 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
RU2723079C2 (ru) 2014-04-22 2020-06-08 Инвайролоджикс, Инк. Композиции и способы для увеличения и/или прогнозирования амплификации днк
KR102465287B1 (ko) 2014-08-11 2022-11-09 루미넥스 코포레이션 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브
EP3180448A4 (en) * 2014-08-14 2018-01-17 Abbott Molecular Inc. Multifunctional oligonucleotides
ES2727656T3 (es) 2014-09-15 2019-10-17 Abvitro Llc Secuenciación de alto rendimiento de banco de nucleótidos
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
EP4141128A1 (en) 2014-10-20 2023-03-01 Envirologix Inc. Compositions and methods for detecting an rna virus
CN114606228B (zh) * 2014-11-20 2025-01-07 杭州智灵龙生物科技有限公司 用于核酸扩增的组合物和方法
WO2016144810A1 (en) * 2015-03-06 2016-09-15 Sigma-Aldrich Co. Llc Nucleic acid amplification and library preparation
WO2017032808A1 (en) * 2015-08-24 2017-03-02 Qiagen Gmbh Method for generating a rna-sequencing library
WO2018057051A1 (en) 2016-09-24 2018-03-29 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
WO2017053903A1 (en) 2015-09-24 2017-03-30 Abvitro Llc Single amplicon activated exclusion pcr
KR20180085717A (ko) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
EP3353550A1 (en) 2015-09-25 2018-08-01 AbVitro LLC High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
CN108367287B (zh) * 2015-11-05 2021-04-02 犹他州大学研究基金会 极端逆转录pcr
JP6744917B2 (ja) * 2015-11-25 2020-08-19 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド バリアント検出のための方法
US20210395799A1 (en) * 2015-11-25 2021-12-23 Integrated Dna Technologies, Inc. Methods for variant detection
US20170218438A1 (en) * 2016-02-01 2017-08-03 Integrated Dna Technologies, Inc. Cleavable primers for isothermal amplification
CN107083427B (zh) * 2016-04-01 2021-07-30 广州市基准医疗有限责任公司 Dna连接酶介导的dna扩增技术
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
WO2018190894A1 (en) 2017-04-13 2018-10-18 Integrated Dna Technologies, Inc. Methods for variant detection
MA49352A (fr) 2017-05-26 2020-04-08 Abvitro Llc Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome
WO2018231985A1 (en) * 2017-06-13 2018-12-20 Genetics Research, Llc, D/B/A Zs Genetics, Inc. Polynucleic acid molecule enrichment methodologies
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
US10527608B2 (en) 2017-06-13 2020-01-07 Genetics Research, Llc Methods for rare event detection
WO2019051335A1 (en) 2017-09-07 2019-03-14 Juno Therapeutics, Inc. METHODS OF IDENTIFYING CELLULAR CHARACTERISTICS RELATED TO RESPONSES ASSOCIATED WITH CELL THERAPY
WO2019067711A1 (en) 2017-09-29 2019-04-04 University Of Iowa Research Foundation DIGITAL NUCLEASE DETECTION COMPOSITIONS AND METHODS
WO2019226734A1 (en) * 2018-05-22 2019-11-28 Biochain Institute, Inc. Target enrichment and sequencing of modified nucleic acid for human cancer detection
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN109913538A (zh) * 2018-09-06 2019-06-21 湖南融健基因生物科技有限公司 一种快速检测snp位点的荧光定量试剂盒
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
KR20210109745A (ko) * 2020-02-28 2021-09-07 주식회사 누리바이오 단일 표적 유전자의 유전적 변이 실시간 검출용 단일핵산 및 이를 이용한 검출 방법
EP4169931A4 (en) * 2020-06-22 2025-05-07 Nuhigh Biotechnologies Co., Ltd Nucleic acid ligand and its use
CN113897420A (zh) * 2020-06-22 2022-01-07 上海思路迪生物医学科技有限公司 基于扩增子测序的大片段重排检测的引物组合物、试剂盒和检测方法
CN112063689A (zh) * 2020-08-21 2020-12-11 上海思路迪生物医学科技有限公司 一种高效rna反转录方法及rna和dna同步建库方法
EP4267762B1 (en) * 2020-12-24 2025-09-17 Integrated DNA Technologies, Inc. Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases
CN113981069B (zh) * 2021-11-10 2023-08-15 郑州华沃生物科技有限公司 检测adrb1基因g1165c多态性的引物、试剂盒及其检测方法和应用
CN115948388A (zh) * 2022-12-30 2023-04-11 纳昂达(南京)生物科技有限公司 特异性捕获引物、靶向捕获探针组合物、靶向捕获文库的构建方法及应用
WO2025096569A1 (en) * 2023-10-30 2025-05-08 The Penn State Research Foundation Methods and compositions for real-time antimicrobial resistance profiling
WO2025207561A1 (en) * 2024-03-27 2025-10-02 Cepheid Isothermal amplification using novel synthetic oligonucleotides
WO2025259863A1 (en) * 2024-06-12 2025-12-18 Orbital Therapeutics, Inc. Thermostable ribonuclease r for circular rna purification

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1340522C (en) 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US6031091A (en) 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5516663A (en) * 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
AU653712B2 (en) 1990-02-16 1994-10-13 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JPH0515439A (ja) 1991-07-08 1993-01-26 Matsushita Electric Ind Co Ltd ジヤー炊飯器の制御回路
JP2001507921A (ja) 1992-04-27 2001-06-19 トラスティーズ オブ ダートマス カレッジ 生物学的液体中の遺伝子配列の検出
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
EP0705905B1 (de) * 1994-07-16 2001-10-10 Roche Diagnostics GmbH Verfahren zum sensitiven Nachweis von Nukleinsäuren
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
EP0803058B1 (en) 1994-12-30 2008-11-05 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5898031A (en) 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
ATE339514T1 (de) 1996-07-16 2006-10-15 Gen Probe Inc Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
DE69827060T2 (de) 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
WO1998046793A1 (en) 1997-04-16 1998-10-22 Trevigen, Inc. Detection and mapping of point mutations using partial digestion
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO1999002728A1 (en) 1997-07-11 1999-01-21 Brax Group Limited Characterising nucleic acids
JPH1132772A (ja) * 1997-07-24 1999-02-09 Mitsubishi Chem Corp 耐熱性リボヌクレアーゼh及びそれをコードするdna
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
AU1707099A (en) 1997-12-04 1999-06-16 Isis Pharmaceuticals, Inc. Human rnase h and compositions and uses thereof
WO2005021776A2 (en) 2003-08-21 2005-03-10 Isis Pharmaceuticals, Inc. METHODS OF USING MAMMALIAN RNase H AND COMPOSITIONS THEREOF
US6248877B1 (en) 1998-04-07 2001-06-19 Biolink Partners Solid phase synthesis of organic compounds via phosphitylating reagents
WO2000053805A1 (en) * 1999-03-10 2000-09-14 Asm Scientific, Inc. A method for direct nucleic acid sequencing
DE60009323T2 (de) 1999-09-13 2005-02-10 Nugen Technologies, Inc., San Carlos Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
AU1239901A (en) 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
EP1925669B1 (en) 2000-02-17 2010-12-08 Qiagen GmbH Thermostable chimeric nucleic acid polymerases and uses thereof
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
AU4162101A (en) 2000-02-23 2001-09-03 Hope City Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
KR100578476B1 (ko) 2000-09-14 2006-05-11 다카라 바이오 가부시키가이샤 내열성 리보뉴클레아제 h
US6858413B2 (en) 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
WO2002064833A1 (en) * 2001-02-15 2002-08-22 Takara Bio Inc. Method of detecting nucleotide polymorphism
US6596489B2 (en) 2001-03-30 2003-07-22 Applied Gene Technologies Methods and compositions for analyzing nucleotide sequence mismatches using RNase H
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
EP1420069A4 (en) 2001-08-20 2005-11-02 Takara Bio Inc nucleic acid amplification
CA2409775C (en) * 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
DE60323611D1 (de) * 2002-03-01 2008-10-30 Integrated Dna Tech Inc Polynomiale amplifikation von nukleinsäuren
US7629152B2 (en) 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
ES2550513T3 (es) * 2002-08-23 2015-11-10 Illumina Cambridge Limited Nucleótidos modificados para secuenciación de polinucleótidos
WO2004020621A1 (ja) * 2002-08-30 2004-03-11 Takara Bio Inc. 耐熱性リボヌクレアーゼh
CN1738914A (zh) 2002-11-21 2006-02-22 震源技术公司 使用编码双链启动子一条链的引物的方法
US20040175737A1 (en) * 2002-12-23 2004-09-09 Wyeth Assay for RNase H activity
JP2005006587A (ja) * 2003-06-20 2005-01-13 Takara Bio Inc 標的核酸の増幅及び/又は検出方法
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
KR101041106B1 (ko) 2003-11-25 2011-06-13 한면기 핵산과 단백질의 신규한 실시간 탐지방법
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
US7344834B2 (en) 2003-12-08 2008-03-18 Cytyc Corporation Method for DNA amplification using DNA blocking probes
EP1715037B1 (en) * 2003-12-10 2010-02-17 Takara Bio Inc. Method of amplifying nucleic acid
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same
JP2008520245A (ja) 2004-11-23 2008-06-19 ワン,シャオ・ビン 切断−増幅法による核酸多様性の検出
WO2006070666A1 (ja) * 2004-12-28 2006-07-06 Takara Bio Inc. 遺伝子多型の同時検出方法
EP2208796B1 (en) * 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
WO2006081426A2 (en) * 2005-01-28 2006-08-03 Applera Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template
US20080003571A1 (en) 2005-02-01 2008-01-03 Mckernan Kevin Reagents, methods, and libraries for bead-based sequencing
US20060211024A1 (en) 2005-03-10 2006-09-21 Gwc Technologies Incorporated Methods for analysis of a nucleic acid sample
US20090068643A1 (en) 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US20070238096A1 (en) 2006-03-09 2007-10-11 The Regents Of The University Of California Hybrid energy transfer for nucleic acid detection
US20070218490A1 (en) 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
DE602007013223D1 (de) 2006-06-01 2011-04-28 Trilink Biotechnologies San Diego Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
GB0611444D0 (en) * 2006-06-09 2006-07-19 Medical Res Council Technology Rnase H2 complex and genes therefor
EP2029780A4 (en) * 2006-06-16 2010-03-31 Pacific Biosciences California CONTROLLED INITIATION OF A PRIMARY EXTENSION
JP2008072427A (ja) 2006-09-14 2008-03-27 Konica Minolta Business Technologies Inc 画像形成装置及びクライアント/サーバ型情報処理システム並びに情報処理方法
EP1921156A1 (en) 2006-11-10 2008-05-14 bioMerieux B.V. Improved multiplex nucleic acid amplification using blocked primers
EP2644709B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
US20110117559A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
US20120052501A1 (en) 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting htlv strains and use thereof
US20120052495A1 (en) 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof

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US20090325169A1 (en) 2009-12-31
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