DK2261230T3 - Fremgangsmåde til proteinoprensning. - Google Patents

Fremgangsmåde til proteinoprensning. Download PDF

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Publication number
DK2261230T3
DK2261230T3 DK10180577.8T DK10180577T DK2261230T3 DK 2261230 T3 DK2261230 T3 DK 2261230T3 DK 10180577 T DK10180577 T DK 10180577T DK 2261230 T3 DK2261230 T3 DK 2261230T3
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DK
Denmark
Prior art keywords
antibody
dna
protein
physiologically active
aqueous solution
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DK10180577.8T
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English (en)
Inventor
Kozo Takeda
Norimichi Ochi
Kimie Ishii
Manabu Matsuhashi
Akinori Imamura
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Chugai Pharmaceutical Co Ltd
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31986593&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2261230(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
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Publication of DK2261230T3 publication Critical patent/DK2261230T3/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3061—Blood cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Water Supply & Treatment (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (14)

1. Fremgangsmåde til fjernelse af vira i en antistofholdig prøve, hvilken fremgangsmåde omfatter følgende trin: 1) at påføre den antistofholdige prøve på en Protein A- eller G-affinitetskromato-grafisøjle; 2) at eluere prøven med en sur vandig opløsning med en lav ledningsevne på 0 til 50 mM; 3) at indstille det fremkomne eluat med en buffer til en pH-værdi svarende til eller lavere end antistoffets isoelektriske punkt, hvor molariteten af det indstillede eluat er 0 til 50 mM; og 4) at fjerne de fremkomne partikler.
2. Fremgangsmåde ifølge krav 1, hvor den sure vandige opløsning med lav ledningsevne har en ledningsevne på 0 til 30 mM som udtrykt i molaritet.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor den sure vandige opløsning med lav ledningsevne har en ledningsevne på 0 til 300 mS/m.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor den sure vandige opløsning er udvalgt blandt vandige opløsninger af saltsyre, citronsyre og eddikesyre.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor pH-vær-dien af det fremkomne eluat indstilles til at svare til eller være lavere end antistoffets isoelektriske punkt og svare til eller være højere end pH 2,0.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, hvor den antistofholdige prøve har vira med en virustiter på 1,03 (logio/ml) eller derunder efter behandling med fjernelse af vira.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, hvor antistoffet er et IgG-antistof.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor antistoffet er et humaniseret monoklonalt antistof.
9. Fremgangsmåde ifølge krav 8, hvor antistoffet er et humaniseret anti-IL-6-receptor-antistof.
10. Fremgangsmåde ifølge krav 8, hvor antistoffet er et humaniseret monoklonalt anti-HM 1.24-antigen-antistof.
11. Fremgangsmåde ifølge krav 8, hvor antistoffet er et humaniseret anti-para-thyroidhormonrelateret peptidantistof (anti-PTHrP-antistof).
12. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 11, hvor partiklerne fjernes ved filtrering gennem et filter.
13. Fremgangsmåde ifølge krav 1, hvor bufferen er en vandig Tris-opløsning.
14. Fremgangsmåde til fremstilling af en medicinsk antistofformulering, hvilken fremgangsmåde omfatter et oprensningstrin, hvori der gøres brug af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 13.
DK10180577.8T 2002-09-11 2003-09-11 Fremgangsmåde til proteinoprensning. DK2261230T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002265609 2002-09-11
EP03795400.5A EP1561756B1 (en) 2002-09-11 2003-09-11 Method of purifying protein

Publications (1)

Publication Number Publication Date
DK2261230T3 true DK2261230T3 (da) 2017-06-12

Family

ID=31986593

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03795400.5T DK1561756T3 (da) 2002-09-11 2003-09-11 Fremgangsmåde til rensning af protein.
DK10180577.8T DK2261230T3 (da) 2002-09-11 2003-09-11 Fremgangsmåde til proteinoprensning.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK03795400.5T DK1561756T3 (da) 2002-09-11 2003-09-11 Fremgangsmåde til rensning af protein.

Country Status (13)

Country Link
US (5) US8420789B2 (da)
EP (3) EP1561756B1 (da)
JP (2) JP5525118B2 (da)
KR (1) KR101119448B1 (da)
CN (1) CN100522989C (da)
AU (1) AU2003262087B2 (da)
CA (1) CA2497364C (da)
DK (2) DK1561756T3 (da)
ES (2) ES2626268T3 (da)
HK (1) HK1077836A1 (da)
HU (1) HUE027134T2 (da)
SI (2) SI2261230T1 (da)
WO (1) WO2004024752A1 (da)

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