DK173699B1 - Fremgangsmåde til fremstilling af en stamme af gæren Kluyveromyces, fremgangsmåde til fremstilling af et polypeptid i Kluyveromyces, anvendelse af Kluyveromyces som vært, transformet Kluyveromyces-celle og Kluyveromyces-ekspressionsvektor, samt plasmider - Google Patents

Fremgangsmåde til fremstilling af en stamme af gæren Kluyveromyces, fremgangsmåde til fremstilling af et polypeptid i Kluyveromyces, anvendelse af Kluyveromyces som vært, transformet Kluyveromyces-celle og Kluyveromyces-ekspressionsvektor, samt plasmider Download PDF

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Publication number
DK173699B1
DK173699B1 DK198400113A DK11384A DK173699B1 DK 173699 B1 DK173699 B1 DK 173699B1 DK 198400113 A DK198400113 A DK 198400113A DK 11384 A DK11384 A DK 11384A DK 173699 B1 DK173699 B1 DK 173699B1
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kluyveromyces
plasmid
dna
transformed
cells
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DK198400113A
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Danish (da)
English (en)
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DK11384A (da
DK11384D0 (da
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Cornelis P Hollenberg
Sunil Das
Albert De Leeuw
Johannes Abel Van Den Berg
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Dsm Nv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
DK198400113A 1982-05-19 1984-01-11 Fremgangsmåde til fremstilling af en stamme af gæren Kluyveromyces, fremgangsmåde til fremstilling af et polypeptid i Kluyveromyces, anvendelse af Kluyveromyces som vært, transformet Kluyveromyces-celle og Kluyveromyces-ekspressionsvektor, samt plasmider DK173699B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NL8202091 1982-05-19
NL8202091 1982-05-19
PCT/EP1983/000128 WO1983004050A1 (en) 1982-05-19 1983-05-19 Cloning system for kluyveromyces species
EP8300128 1983-05-19

Publications (3)

Publication Number Publication Date
DK11384A DK11384A (da) 1984-01-11
DK11384D0 DK11384D0 (da) 1984-01-11
DK173699B1 true DK173699B1 (da) 2001-07-02

Family

ID=19839766

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198400113A DK173699B1 (da) 1982-05-19 1984-01-11 Fremgangsmåde til fremstilling af en stamme af gæren Kluyveromyces, fremgangsmåde til fremstilling af et polypeptid i Kluyveromyces, anvendelse af Kluyveromyces som vært, transformet Kluyveromyces-celle og Kluyveromyces-ekspressionsvektor, samt plasmider

Country Status (20)

Country Link
US (2) US4657857A (de)
EP (2) EP0096430B2 (de)
JP (3) JPS59501572A (de)
AR (1) AR241796A1 (de)
AT (2) ATE49421T1 (de)
AU (1) AU571181B2 (de)
BR (1) BR8307525A (de)
CA (1) CA1224735A (de)
DE (2) DE3381090D1 (de)
DK (1) DK173699B1 (de)
ES (1) ES8402874A1 (de)
FI (1) FI81379C (de)
GR (1) GR78852B (de)
HU (1) HU204097B (de)
IE (1) IE55817B1 (de)
IL (1) IL68733A (de)
NO (1) NO172397C (de)
NZ (1) NZ204302A (de)
PT (1) PT76727B (de)
WO (2) WO1983004050A1 (de)

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Also Published As

Publication number Publication date
NZ204302A (en) 1987-03-06
JPS61275227A (ja) 1986-12-05
HU204097B (en) 1991-11-28
DK11384A (da) 1984-01-11
FI81379B (fi) 1990-06-29
ATE42338T1 (de) 1989-05-15
EP0096430B1 (de) 1990-01-10
NO840133L (no) 1984-01-13
WO1983004051A1 (en) 1983-11-24
GR78852B (de) 1984-10-02
DE3381090D1 (de) 1990-02-15
AR241796A1 (es) 1992-12-30
IE55817B1 (en) 1991-01-30
JP2608540B2 (ja) 1997-05-07
CA1224735A (en) 1987-07-28
EP0096910B1 (de) 1989-04-19
NO172397C (no) 1993-07-14
EP0096430B2 (de) 1998-04-01
US4859596A (en) 1989-08-22
PT76727A (en) 1983-06-01
IL68733A (en) 1989-01-31
EP0096430A1 (de) 1983-12-21
FI81379C (fi) 1990-10-10
US4657857A (en) 1987-04-14
DE3379665D1 (en) 1989-05-24
WO1983004050A1 (en) 1983-11-24
NO172397B (no) 1993-04-05
ATE49421T1 (de) 1990-01-15
PT76727B (en) 1985-12-27
FI840149A (fi) 1984-01-16
AU1553983A (en) 1983-12-02
ES522556A0 (es) 1984-03-01
JPS59500848A (ja) 1984-05-17
FI840149A0 (fi) 1984-01-16
DK11384D0 (da) 1984-01-11
AU571181B2 (en) 1988-04-14
ES8402874A1 (es) 1984-03-01
IE831185L (en) 1983-11-19
JPS59501572A (ja) 1984-09-06
EP0096910A1 (de) 1983-12-28
JPH0543348B2 (de) 1993-07-01
JPH0685720B2 (ja) 1994-11-02
BR8307525A (pt) 1984-08-21

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