DK0550687T3 - 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser - Google Patents

5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser

Info

Publication number
DK0550687T3
DK0550687T3 DK91919358T DK91919358T DK0550687T3 DK 0550687 T3 DK0550687 T3 DK 0550687T3 DK 91919358 T DK91919358 T DK 91919358T DK 91919358 T DK91919358 T DK 91919358T DK 0550687 T3 DK0550687 T3 DK 0550687T3
Authority
DK
Denmark
Prior art keywords
thermostable dna
sequence
seq
dna
dna polymerases
Prior art date
Application number
DK91919358T
Other languages
Danish (da)
English (en)
Inventor
David H Gelfand
Richard D Abramson
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0550687T3 publication Critical patent/DK0550687T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK91919358T 1990-09-28 1991-09-30 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser DK0550687T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US59046690A 1990-09-28 1990-09-28
US59021390A 1990-09-28 1990-09-28
US59049090A 1990-09-28 1990-09-28
PCT/US1991/007035 WO1992006200A1 (en) 1990-09-28 1991-09-30 5' to 3' exonuclease mutations of thermostable dna polymerases

Publications (1)

Publication Number Publication Date
DK0550687T3 true DK0550687T3 (da) 1999-12-13

Family

ID=27416562

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91919358T DK0550687T3 (da) 1990-09-28 1991-09-30 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser

Country Status (10)

Country Link
EP (2) EP0894860B1 (de)
JP (4) JP2709311B2 (de)
AT (1) ATE181106T1 (de)
AU (2) AU663474B2 (de)
CA (1) CA2090614C (de)
DE (1) DE69131321T2 (de)
DK (1) DK0550687T3 (de)
ES (1) ES2134198T3 (de)
GR (1) GR3031152T3 (de)
WO (1) WO1992006200A1 (de)

Families Citing this family (172)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2582980B2 (ja) * 1990-09-28 1997-02-19 エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト モルモシポ・アフリカヌスからの純化された熱安定性核酸ポリメラーゼ
JPH06502303A (ja) * 1990-10-05 1994-03-17 バーネス,ウェーン・エム 熱安定性dnaポリメラーゼ
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US6673616B1 (en) 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US6372424B1 (en) 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US7785852B2 (en) 1992-12-07 2010-08-31 Third Wave Technologies, Inc. Cleavage of nucleic acids
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5888780A (en) * 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US6410277B1 (en) 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
US7312052B1 (en) 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
DK0789575T3 (da) 1994-11-02 2009-01-12 Nps Pharma Inc Perifer-nerve-system-specifikke natriumkanaler, DNA kodende derfor, screening af lægemidler og fremgangsmåder til fremstilling og anvendelse deraf
WO1996014405A2 (en) * 1994-11-04 1996-05-17 Molecular Biology Resources, Inc. Biologically active fragments of thermus flavus dna polymerase
CA2211316C (en) * 1995-02-03 2013-10-01 Novo Nordisk A/S Method of designing alpha-amylase mutants with predetermined properties
US6440716B1 (en) 1995-02-03 2002-08-27 Novozymes A/S α-amylase mutants
CA2222744C (en) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5770690A (en) * 1995-06-27 1998-06-23 Neurex Corporation Bax omega protein and methods
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
JP2000502882A (ja) * 1995-09-08 2000-03-14 ライフ・テクノロジーズ・インコーポレイテッド サーモトガ由来のクローン化dnaポリメラーゼ類およびそれらの変異体
EP0866868B1 (de) * 1995-12-15 2001-07-11 Amersham Life Science Inc Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US7195871B2 (en) 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
AU4065397A (en) 1996-08-14 1998-03-06 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
DK0966542T3 (da) 1996-11-29 2009-03-23 Third Wave Tech Inc FEN-1 endonukleaser, blandinger og spaltningsmethoder
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1998042860A1 (en) 1997-03-21 1998-10-01 Stratagene Polymerase enhancing factor (pef) extracts, pef protein complexes, isolated pef protein, and methods for purifying and identifying
DK1705253T3 (da) 1997-04-03 2011-02-21 Life Technologies Corp Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)
AU7360198A (en) 1997-04-22 1998-11-13 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6140086A (en) 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
AU9123598A (en) 1997-08-29 1999-03-16 Life Technologies, Inc. High fidelity polymerases and uses thereof
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
EP0921196A1 (de) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modifizierte DNA Polymerase aus Carboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-kettenreaktion
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
US7166745B1 (en) 1998-11-12 2007-01-23 Invitrogen Corporation Transfection reagents
WO2000071757A1 (en) 1999-05-21 2000-11-30 Invitrogen Corporation Compositions and methods for labeling of nucleic acid molecules
US7537886B1 (en) 1999-06-22 2009-05-26 Life Technologies Corporation Primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
EP1250453B1 (de) 1999-12-10 2008-04-09 Invitrogen Corporation Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
DE60133992D1 (de) 2000-02-17 2008-06-26 Qiagen Gmbh Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
CA2406939A1 (en) 2000-05-26 2001-12-06 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
EP1301205A1 (de) * 2000-07-12 2003-04-16 Invitrogen Corporation Hochempfindliche polymerasen und ihre verwendungen
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE10049211A1 (de) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
CA2428798C (en) 2000-11-15 2013-09-03 Third Wave Technologies, Inc. Fen-1 endonucleases from archaeoglobus veneficus
WO2002046372A1 (en) 2000-12-08 2002-06-13 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
JP2005511044A (ja) * 2001-11-30 2005-04-28 アプレラ コーポレイション Thermusoshimai核酸ポリメラーゼ
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
EP1540009B1 (de) 2002-08-05 2011-09-28 Quanta Biosciences, Inc. Verbesserte zusammensetzungen zur in-vitro-amplifikation von nukleinsäuren
EP2325303B1 (de) 2002-09-13 2013-11-06 Life Technologies Corporation Wärmestabile reverse Transkriptasen und Verwendungen davon
CA2498764C (en) * 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7704712B2 (en) 2003-03-25 2010-04-27 Stratagene California DNA polymerase fusions and uses thereof
ES2524048T3 (es) 2003-03-31 2014-12-03 F. Hoffmann-La Roche Ag Composiciones y métodos para la detección de ciertos flavivirus, incluidos los miembros del serogrupo del virus de la encefalitis japonesa
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
JP4896006B2 (ja) 2004-04-08 2012-03-14 バイオマトリカ, インコーポレイテッド ライフサイエンスのためのサンプル保存とサンプル管理との統合
US20060024757A1 (en) 2004-07-30 2006-02-02 Robert Hussa Detection of oncofetal fibronectin for selection of concepti
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
JP2007104909A (ja) * 2005-10-11 2007-04-26 Tama Tlo Kk 好熱性細菌サーマス・カワライェンシス由来耐熱性dnaポリメラーゼ
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
CA2639416C (en) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Diagnostic test for susceptibility to b-raf kinase inhibitors
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
US20090253142A1 (en) 2008-03-15 2009-10-08 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
EP2392680A1 (de) 2010-06-04 2011-12-07 Labor Diagnostik GmbH Leipzig Verfahren zum spezifischen Nachweis des klassischen Schweinepestvirus
US8722378B2 (en) 2010-06-18 2014-05-13 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
CA2802000C (en) 2010-06-18 2016-08-16 F. Hoffmann-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
WO2011157435A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
US8735120B2 (en) 2010-06-18 2014-05-27 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157430A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CA2802304C (en) 2010-06-18 2016-01-12 F.Hoffman-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
JP5876479B2 (ja) 2010-06-18 2016-03-02 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別を有するdnaポリメラーゼ
WO2011163249A2 (en) 2010-06-21 2011-12-29 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
EP2582839B1 (de) 2010-06-21 2016-05-18 Life Technologies Corporation Zusammensetzungen, verfahren und kits zur synthese und amplifikation von nukleinsäuren mit rt
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
US20120058481A1 (en) 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
US10301674B2 (en) 2010-08-20 2019-05-28 Life Technologies Corporation Polymerase assay with a FRET substrate
JP5847201B2 (ja) 2011-02-15 2016-01-20 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別能を有するdnaポリメラーゼ
WO2012139748A1 (en) 2011-04-11 2012-10-18 Roche Diagnostics Gmbh Dna polymerases with improved activity
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
EP2710146A2 (de) 2011-05-18 2014-03-26 Life Technologies Corporation Chromosomenkonformationsanalyse
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
JP5830286B2 (ja) * 2011-07-12 2015-12-09 アークレイ株式会社 核酸の増幅検出方法およびキット
JP5977000B2 (ja) 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
WO2013008898A1 (ja) * 2011-07-12 2013-01-17 国立大学法人九州大学 新規dnaポリメラーゼ
US9090883B2 (en) 2011-07-28 2015-07-28 Roche Molecular Systems, Inc. DNA polymerases with improved activity
CN107326079B (zh) 2011-08-10 2021-04-20 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
ES2569723T3 (es) 2011-12-08 2016-05-12 F. Hoffmann-La Roche Ag Polimerasas de DNA con actividad mejorada
EP2788480B1 (de) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
EP3366769B1 (de) 2012-10-16 2021-02-17 DNA Polymerase Technology, Inc. Hemmungsresistente polymerasen
EP2914741B1 (de) 2012-11-02 2017-08-16 Life Technologies Corporation Neuartige zusammensetzungen und verfahren zur verstärkung der pcr-spezifität
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
WO2014165210A2 (en) 2013-03-12 2014-10-09 Life Technologies Corporation Universal reporter-based genotyping methods and materials
US9828629B2 (en) 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Pcrシステムにおいて使用するための新規化合物及びその用途
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
US20150176060A1 (en) 2013-12-20 2015-06-25 Roche Molecular Systems, Inc. Method For Coding Of Multiple PCR Reactions For Assay Recognition
US9243289B2 (en) 2013-12-23 2016-01-26 Roche Molecular Systems, Inc. Method for screening reagents used in PCR assays
US20160053302A1 (en) 2014-04-22 2016-02-25 Roche Molecular Systems, Inc. Method for visual identification of pcr solutions for accurate reaction setup
CN106572650B (zh) 2014-06-10 2021-08-31 生物马特里卡公司 在环境温度下稳定凝血细胞
CN106572974B (zh) 2014-07-15 2021-04-23 生命技术公司 用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016126987A1 (en) * 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US10563250B2 (en) 2015-03-13 2020-02-18 Life Technologies Corporation Methods, compositions and kits for small RNA capture, detection and quantification
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
EP4141126A1 (de) 2015-10-01 2023-03-01 Life Technologies Corporation Polymerasezusammensetzungen und kits sowie verfahren zur verwendung und herstellung davon
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
EP4242628A3 (de) 2015-12-08 2023-11-08 Biomatrica, INC. Verringerung der erythrozytensedimentationsrate
CN109072291A (zh) 2016-04-06 2018-12-21 生命技术公司 用于合成和检测核酸的组合物、方法和试剂盒
WO2017201315A1 (en) 2016-05-18 2017-11-23 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
US20170362640A1 (en) 2016-06-16 2017-12-21 Life Technologies Corporation Novel compositions, methods and kits for microorganism detection
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11613777B2 (en) 2016-08-26 2023-03-28 Life Technologies Corporation Nucleic acid extraction and amplification controls and methods of use thereof
KR102468174B1 (ko) 2016-09-15 2022-11-17 에프. 호프만-라 로슈 아게 멀티플렉스 pcr 수행 방법
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592853A1 (de) 2017-03-09 2020-01-15 President and Fellows of Harvard College Unterdrückung von schmerzen durch geneditierung
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019094973A1 (en) 2017-11-13 2019-05-16 Life Technologies Corporation Compositions, methods and kits for urinary tract microorganism detection
JP2022500046A (ja) 2018-09-13 2022-01-04 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改善されたストランド置換能力を有する変異体dnaポリメラーゼ
WO2020127620A1 (en) 2018-12-20 2020-06-25 F. Hoffmann-La Roche Ag Detection of target nucleic acid by solid-phase molography
WO2020191153A2 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
KR20230019843A (ko) 2020-05-08 2023-02-09 더 브로드 인스티튜트, 인코퍼레이티드 표적 이중 가닥 뉴클레오티드 서열의 두 가닥의 동시 편집을 위한 방법 및 조성물
US20230265531A1 (en) 2020-07-22 2023-08-24 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
WO2022067130A2 (en) 2020-09-24 2022-03-31 The Broad Institute, Inc. Prime editing guide rnas, compositions thereof, and methods of using the same
JP2023543602A (ja) 2020-10-06 2023-10-17 キージーン ナムローゼ フェンノートシャップ 標的化された配列付加
EP4267764A1 (de) 2020-12-22 2023-11-01 F. Hoffmann-La Roche AG Verfahren zur durchführung von multiplexierter echtzeit-pcr unter verwendung von grossen stokes-verschiebungsfluoreszenzfarbstoffen
WO2022150790A2 (en) 2021-01-11 2022-07-14 The Broad Institute, Inc. Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
WO2023076898A1 (en) 2021-10-25 2023-05-04 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
WO2023104812A1 (en) 2021-12-09 2023-06-15 F. Hoffmann-La Roche Ag Mass based detection of pcr amplicons
WO2024108092A1 (en) 2022-11-17 2024-05-23 The Broad Institute, Inc. Prime editor delivery by aav

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
DE69032543T2 (de) * 1989-12-22 1999-04-15 Hoffmann La Roche Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus
DE69030386T2 (de) * 1989-12-22 1997-10-09 Hoffmann La Roche Bei hoher temperatur aktive reverse transkriptasen
JPH06502303A (ja) * 1990-10-05 1994-03-17 バーネス,ウェーン・エム 熱安定性dnaポリメラーゼ

Also Published As

Publication number Publication date
JPH104985A (ja) 1998-01-13
JP2886842B2 (ja) 1999-04-26
DE69131321T2 (de) 1999-12-16
JPH05506364A (ja) 1993-09-22
ATE181106T1 (de) 1999-06-15
AU8668891A (en) 1992-04-28
AU691374B2 (en) 1998-05-14
AU4086896A (en) 1996-04-26
JPH1095A (ja) 1998-01-06
JP3227102B2 (ja) 2001-11-12
EP0894860B1 (de) 2012-12-12
WO1992006200A1 (en) 1992-04-16
AU663474B2 (en) 1995-10-12
JP3227101B2 (ja) 2001-11-12
GR3031152T3 (en) 1999-12-31
EP0550687A1 (de) 1993-07-14
DE69131321D1 (de) 1999-07-15
EP0894860A1 (de) 1999-02-03
CA2090614A1 (en) 1992-03-29
CA2090614C (en) 2001-12-11
ES2134198T3 (es) 1999-10-01
JP2709311B2 (ja) 1998-02-04
JPH104965A (ja) 1998-01-13
EP0550687B1 (de) 1999-06-09

Similar Documents

Publication Publication Date Title
DK0550687T3 (da) 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser
DE69836779D1 (de) Mutierte chimäre DNS-Polymerasen
DE69426400D1 (de) Thermostable Nukleinsäure Polymerase
DK0823479T3 (da) Modificeret termostabil DNA-polymerase
WO1999020766A3 (en) NOVEL MODIFIED NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS
WO1992006188A3 (en) Thermostable dna polymerase
AU3062989A (en) Purified thermostable enzyme
DK0693078T3 (da) DNA-polymeraser med forøget termostabilitet og forøget længde og effektivitet af primerforlængelse
DE69133629D1 (de) nssystem zur Bestimmung von genetischen Krankheiten
ES2173867T3 (es) Uso de nucleotidos externos a la muestra en clonacion genetica.
NO20015587D0 (no) Ekspresjon og eksport av interferon-<alfa> proteiner som Fc- fusjonsproteiner
BG100188A (en) Dna sequence enzymes
FI934812A (fi) Stabiliserade entzymer
EP0875576A3 (de) Bacillus stearothermophilus DNS Polymerase I (Klenow) Klone einschliesslich derjenigen mit reduzierter 3' - 5' Exonuklease Aktivität
ATE346937T1 (de) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius