ES2173867T3 - Uso de nucleotidos externos a la muestra en clonacion genetica. - Google Patents

Uso de nucleotidos externos a la muestra en clonacion genetica.

Info

Publication number
ES2173867T3
ES2173867T3 ES92914436T ES92914436T ES2173867T3 ES 2173867 T3 ES2173867 T3 ES 2173867T3 ES 92914436 T ES92914436 T ES 92914436T ES 92914436 T ES92914436 T ES 92914436T ES 2173867 T3 ES2173867 T3 ES 2173867T3
Authority
ES
Spain
Prior art keywords
clonation
nucleotides
genetic
sample
external
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92914436T
Other languages
English (en)
Inventor
Mark S Berninger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ES2173867T3 publication Critical patent/ES2173867T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

LA INVENCION SUMINISTRA METODOS MEJORADOS PARA MANIPULAR DNA RECOMBINANTE EN LA CLONACION Y EXPRESION DE GENES. MAS ESPECIFICAMENTE, LA INVENCION SUMINISTRA METODOS CAPACES DE ALTERAR UNA SECUENCIA DE ACIDO NUCLEICO PRESENTE EN EL TERMINAL DE UNA SECUENCIA DESEADA.
ES92914436T 1991-06-14 1992-06-12 Uso de nucleotidos externos a la muestra en clonacion genetica. Expired - Lifetime ES2173867T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/715,623 US5137814A (en) 1991-06-14 1991-06-14 Use of exo-sample nucleotides in gene cloning

Publications (1)

Publication Number Publication Date
ES2173867T3 true ES2173867T3 (es) 2002-11-01

Family

ID=24874814

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92914436T Expired - Lifetime ES2173867T3 (es) 1991-06-14 1992-06-12 Uso de nucleotidos externos a la muestra en clonacion genetica.

Country Status (9)

Country Link
US (1) US5137814A (es)
EP (2) EP0544894B1 (es)
JP (1) JP3395905B2 (es)
AT (1) ATE214095T1 (es)
CA (1) CA2088766A1 (es)
DE (1) DE69232457T2 (es)
DK (1) DK0544894T3 (es)
ES (1) ES2173867T3 (es)
WO (1) WO1992022649A1 (es)

Families Citing this family (50)

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US5683896A (en) * 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5229283A (en) * 1991-06-14 1993-07-20 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
WO1993018175A1 (en) * 1992-03-10 1993-09-16 Life Technologies, Inc. Udg-facilitated mutagenesis
US5426039A (en) * 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
US6680378B1 (en) * 1993-10-28 2004-01-20 Invitrogen Corporation Nucleic acid marker ladder for estimating mass
ES2118534T3 (es) * 1993-10-28 1998-09-16 Life Technologies Inc Escalera de marcadores de acido nucleico para estimacion de la masa.
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
AU3399797A (en) * 1996-06-17 1998-01-07 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
IL120339A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6365731B1 (en) 1997-08-06 2002-04-02 Ambion, Inc. Stripping nucleic acids with iodine and sodium thiosulfate
US6815185B2 (en) * 1997-11-17 2004-11-09 Robert D. Klein Methods of creating constructs useful for introducing sequences into embryonic stem cells
CA2374515A1 (en) 1999-05-21 2000-11-30 Christian E. Gruber Compositions and methods for labeling of nucleic acid molecules
EP1190097A2 (en) 1999-06-22 2002-03-27 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) * 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6998273B1 (en) * 2000-02-09 2006-02-14 A-Fem Medical Corporation Collection device for lateral flow chromatography
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US7435572B2 (en) 2002-04-12 2008-10-14 New England Biolabs, Inc. Methods and compositions for DNA manipulation
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
WO2005049848A2 (en) * 2003-11-12 2005-06-02 Geneohm Sciences, Inc. Nucleic acid hybridization methods
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
US7754944B2 (en) * 2006-04-03 2010-07-13 Monsanto Technology Llc Polynucleotide constructs
CN101979592B (zh) * 2010-10-27 2012-05-09 上海交通大学 固氮鱼腥藻乙酰乳酸合酶基因的核苷酸序列
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
DK3030682T3 (da) 2013-08-05 2020-09-14 Twist Bioscience Corp De novo synthesized gene libraries
WO2016022557A1 (en) * 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016183100A1 (en) * 2015-05-11 2016-11-17 Twist Bioscience Corporation Compositions and methods for nucleic acid amplification
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods to regulate the activity of proteins and cells and a method for the production of nucleic acids
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND THEIR PREPARATION
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN118116478A (zh) 2017-02-22 2024-05-31 特韦斯特生物科学公司 基于核酸的数据存储
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
WO2019136175A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based digital information storage
AU2019270243A1 (en) 2018-05-18 2021-01-07 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CN113766930A (zh) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Glp1受体的变异核酸文库
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873192A (en) * 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
EP0397463A3 (en) * 1989-05-10 1991-03-06 Eastman Kodak Company Construction of synthetic double-stranded dna sequences
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions

Also Published As

Publication number Publication date
DK0544894T3 (da) 2002-04-22
DE69232457T2 (de) 2002-10-10
DE69232457D1 (de) 2002-04-11
ATE214095T1 (de) 2002-03-15
CA2088766A1 (en) 1992-12-15
EP0544894A1 (en) 1993-06-09
JPH06500702A (ja) 1994-01-27
EP1156108A3 (en) 2001-12-12
US5137814A (en) 1992-08-11
EP1156108A2 (en) 2001-11-21
EP0544894B1 (en) 2002-03-06
JP3395905B2 (ja) 2003-04-14
EP0544894A4 (en) 1994-10-19
WO1992022649A1 (en) 1992-12-23

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