ES2059431T3 - Mutantes de la creatinamidinohidrolasa estables. - Google Patents

Mutantes de la creatinamidinohidrolasa estables.

Info

Publication number
ES2059431T3
ES2059431T3 ES88107636T ES88107636T ES2059431T3 ES 2059431 T3 ES2059431 T3 ES 2059431T3 ES 88107636 T ES88107636 T ES 88107636T ES 88107636 T ES88107636 T ES 88107636T ES 2059431 T3 ES2059431 T3 ES 2059431T3
Authority
ES
Spain
Prior art keywords
creatinamidinohydrolase
stable
wild
mutants
type enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88107636T
Other languages
English (en)
Inventor
Gunther Dr Rer Nat Schumacher
Peter Dr Rer Nat Buckel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2059431T3 publication Critical patent/ES2059431T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas
    • Y10S435/877—Pseudomonas putida

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

UNA NUEVA CREATINAMIDINOHIDROLASA , EN LA QUE EN LA POSICION 109 DE LA SECUENCIA DE AMINOACIDOS DE LA ENZIMA DE TIPO SALVAJE, LA ALANINA SE SUSTITUYE MEDIANTE VALINA Y, SI ES CASO, ADEMAS UNO O VARIOS AMINOACIDOS DE ANZIMA DE TIPO SALVAJE, SUFREN MUTACION, ES MAS ESTABLE QUE LA ENZIMA DE TIPO SALVAJE Y ES MAS APROPIADA PARA LOS REACTIVOS. PARA SU OBTENCION, SE ACUDE A METODOS DE TECNOLOGIA GENETICA CONOCIDOS EN UN SISTEMA DE EXPRESION APROPIADO PARA LLEVAR AL ADN RECOMBINANTE A EXPRESION, QUE CONTIENE UN GEN DE CREATINAMIDINOHIDROLASA, QUE CONTIENE EN SU POSICION 326 DEL GEN NATURAL EL DESOXIRIBONUCLEOTIDA, EN LUGAR DE C.
ES88107636T 1987-05-12 1988-05-11 Mutantes de la creatinamidinohidrolasa estables. Expired - Lifetime ES2059431T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3715841 1987-05-12
DE3803175A DE3803175A1 (de) 1987-05-12 1988-02-03 Stabile creatinamidinohydrolase-mutanten

Publications (1)

Publication Number Publication Date
ES2059431T3 true ES2059431T3 (es) 1994-11-16

Family

ID=25855502

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88107636T Expired - Lifetime ES2059431T3 (es) 1987-05-12 1988-05-11 Mutantes de la creatinamidinohidrolasa estables.

Country Status (12)

Country Link
US (1) US4990453A (es)
EP (1) EP0291055B1 (es)
JP (1) JPS63287484A (es)
KR (1) KR900007648B1 (es)
AU (1) AU593438B2 (es)
CA (1) CA1339103C (es)
CZ (1) CZ279006B6 (es)
DE (2) DE3803175A1 (es)
DK (1) DK251788A (es)
ES (1) ES2059431T3 (es)
HU (1) HU204093B (es)
IE (1) IE60307B1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6437292A (en) * 1987-08-04 1989-02-07 Toyo Jozo Kk Dna having genetic information of creatinase and use thereof
JP2000157279A (ja) 1998-11-25 2000-06-13 Kikkoman Corp クレアチンアミジノハイドロラーゼ及びその製造法
JP3773160B2 (ja) * 1999-01-01 2006-05-10 キッコーマン株式会社 耐熱性クレアチンアミジノハイドロラーゼ及びその製造法
CA2404293C (en) 2001-09-20 2007-05-15 F. Hoffmann-La Roche Ag Variants of an erwinia-type creatinase

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2122255B2 (de) * 1971-05-05 1979-01-11 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur spezifischen Bestimmung von Creatinin
US4039384A (en) * 1975-04-05 1977-08-02 Noda Institute For Scientific Research Creatinine amidohydrolase and creatine amidinohydrolase and process for producing them
JPS5736985A (ja) * 1980-08-14 1982-02-27 Toyobo Co Ltd Kosoanteikaho
JPS59232088A (ja) * 1983-06-15 1984-12-26 Tanabe Seiyaku Co Ltd Ｌ−アスパラギン酸の製法
DE3500184A1 (de) * 1985-01-04 1986-08-14 Boehringer Mannheim Gmbh, 6800 Mannheim Mikroorganismus und plasmid fuer konstitutive creatinamidinohydrolase-bildung sowie verfahren zur herstellung derselben
DE3707172C2 (de) * 1986-03-07 1995-09-21 Kikkoman Corp Rekombiniertes DNA-Molekül, das ein Kreatinasegen codiert, rekombinanter Vektor, der diese DNA-Sequenz umfaßt, sowie Verfahren zur Herstellung von Kreatinase unter Verwendung eines Stammes vom Genus Escherichia, der diesen rekombinanten DNA Vektor enthält

Also Published As

Publication number Publication date
DK251788A (da) 1988-11-13
DE3884045D1 (de) 1993-10-21
CZ279006B6 (en) 1994-11-16
JPH0336514B2 (es) 1991-05-31
EP0291055A3 (en) 1989-11-29
IE60307B1 (en) 1994-06-29
US4990453A (en) 1991-02-05
DK251788D0 (da) 1988-05-06
KR880014106A (ko) 1988-12-22
EP0291055B1 (de) 1993-09-15
EP0291055A2 (de) 1988-11-17
IE881016L (en) 1988-11-12
JPS63287484A (ja) 1988-11-24
CA1339103C (en) 1997-07-29
HUT46942A (en) 1988-12-28
CZ311888A3 (en) 1994-04-13
HU204093B (en) 1991-11-28
AU1518688A (en) 1988-11-17
KR900007648B1 (ko) 1990-10-17
AU593438B2 (en) 1990-02-08
DE3803175A1 (de) 1988-11-24

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