ATE530652T1 - Fen-1-endonucleasen, mischungen und spaltungsverfahren - Google Patents

Fen-1-endonucleasen, mischungen und spaltungsverfahren

Info

Publication number
ATE530652T1
ATE530652T1 AT08019704T AT08019704T ATE530652T1 AT E530652 T1 ATE530652 T1 AT E530652T1 AT 08019704 T AT08019704 T AT 08019704T AT 08019704 T AT08019704 T AT 08019704T AT E530652 T1 ATE530652 T1 AT E530652T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid sequences
fen
endonucleases
mixtures
Prior art date
Application number
AT08019704T
Other languages
English (en)
Inventor
Michael W Kaiser
Victor I Lyamichev
Natasha Lyamicheva
Original Assignee
Third Wave Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/757,653 external-priority patent/US5843669A/en
Priority claimed from US08/758,314 external-priority patent/US6090606A/en
Application filed by Third Wave Tech Inc filed Critical Third Wave Tech Inc
Application granted granted Critical
Publication of ATE530652T1 publication Critical patent/ATE530652T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT08019704T 1996-11-29 1997-11-26 Fen-1-endonucleasen, mischungen und spaltungsverfahren ATE530652T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/757,653 US5843669A (en) 1996-01-24 1996-11-29 Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
US08/758,314 US6090606A (en) 1996-01-24 1996-12-02 Cleavage agents

Publications (1)

Publication Number Publication Date
ATE530652T1 true ATE530652T1 (de) 2011-11-15

Family

ID=27116427

Family Applications (2)

Application Number Title Priority Date Filing Date
AT08019704T ATE530652T1 (de) 1996-11-29 1997-11-26 Fen-1-endonucleasen, mischungen und spaltungsverfahren
AT97952237T ATE414167T1 (de) 1996-11-29 1997-11-26 Fen-1 endonukleasen, gemische und spaltmethoden

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT97952237T ATE414167T1 (de) 1996-11-29 1997-11-26 Fen-1 endonukleasen, gemische und spaltmethoden

Country Status (10)

Country Link
US (2) US6562611B1 (de)
EP (3) EP2055781B1 (de)
JP (4) JP4362150B2 (de)
AT (2) ATE530652T1 (de)
AU (1) AU737449B2 (de)
CA (1) CA2273204C (de)
DE (1) DE69739108D1 (de)
DK (1) DK0966542T3 (de)
ES (2) ES2375764T3 (de)
WO (1) WO1998023774A1 (de)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1998023774A1 (en) * 1996-11-29 1998-06-04 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
US6210880B1 (en) 1997-09-19 2001-04-03 Third Wave Technologies, Inc. Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
JP2001523111A (ja) 1997-05-05 2001-11-20 サード ウェーブ テクノロジーズ,インコーポレーテッド 構造体架橋オリゴヌクレオチドを使用した標的依存型反応
CA2375060A1 (en) * 1999-05-24 2000-11-30 Life Technologies Corporation Method for deblocking of labeled oligonucleotides
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US7534568B2 (en) 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
WO2001061015A2 (de) * 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
WO2001071044A1 (en) * 2000-03-22 2001-09-27 Quantum Dot Corporation Methods of using semiconductor nanocrystals in bead-based nucleic acid assays
WO2001073127A2 (en) * 2000-03-27 2001-10-04 Applera Corporation Improved invasion assay
JP2004521606A (ja) * 2000-05-24 2004-07-22 サード・ウェーブ・テクノロジーズ・インク Rnaの検出法
JP2004507230A (ja) 2000-07-03 2004-03-11 アプレラ コーポレイション ポリヌクレオチド配列アッセイ
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
CA2428798C (en) * 2000-11-15 2013-09-03 Third Wave Technologies, Inc. Fen-1 endonucleases from archaeoglobus veneficus
DE10131441A1 (de) * 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7312033B2 (en) 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
EP1573009B1 (de) 2002-12-18 2011-09-21 Third Wave Technologies, Inc. Nachweis kleiner nukleinsäuren
US7700750B2 (en) 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
US20040161749A1 (en) 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
AU2004259952B2 (en) 2003-07-29 2008-10-16 Otsuka Pharmaceutical Co., Ltd. Method of judging risk for onset of drug-induced granulocytopenia
WO2005067643A2 (en) 2004-01-07 2005-07-28 Third Wave Technologies, Inc. Determination of hepatitis c virus genotype
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
CN102242196A (zh) 2004-10-28 2011-11-16 大塚制药株式会社 对干扰素治疗肾细胞癌反应的鉴定标记
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
WO2007044727A2 (en) 2005-10-07 2007-04-19 California Institute Of Technology Pkr activation via hybridization chain reaction
US7482127B2 (en) 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
WO2007111324A1 (ja) * 2006-03-29 2007-10-04 Kyoto University 遺伝子中の5-メチルシトシン検出方法および検出キット
EP2522751B1 (de) 2006-06-01 2018-11-21 Third Wave Technologies, Inc. Nachweis von Nukleinsäuren durch einem verbesserten INVADER-assay
US7759062B2 (en) 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
WO2008045251A2 (en) 2006-10-04 2008-04-17 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8497364B2 (en) 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
WO2009117327A2 (en) 2008-03-15 2009-09-24 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
WO2009155271A1 (en) * 2008-06-17 2009-12-23 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
EP2691543B1 (de) 2011-03-29 2017-11-01 Seegene, Inc. Erkennung von zielnukleinsäuresequenzen durch pto-spaltung und verlängerungsabhängige spaltung
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
WO2013058868A2 (en) 2011-10-18 2013-04-25 Exact Sciences Corporation Multiplexed kras mutation detection assay
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
JP5976849B2 (ja) * 2012-03-05 2016-08-24 シージーン アイエヌシー PTO切断及び伸長アッセイによるターゲット核酸配列でのヌクレオチド変異検出{DetectionofNucleotideVariationonTargetNucleicAcidSequencebyPTOCleavageandExtensionAssay}
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
US9856472B2 (en) 2013-07-01 2018-01-02 California Institute Of Technology Small conditional RNAs
TWI707038B (zh) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
AU2016257426B2 (en) 2015-05-01 2020-01-16 Gen-Probe Incorporated Multiplex invasive cleavage assays
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
IL324487A (en) 2016-07-05 2026-01-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
CA3034617A1 (en) 2016-08-30 2018-03-08 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
KR102514213B1 (ko) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 면역 시냅스의 변이체 라이브러리 및 그의 합성
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN107012241B (zh) * 2017-05-09 2020-11-20 东莞微量精准检测研究院有限公司 一种U6、miR 92a及miR 21的三重RT-qPCR检测方法及试剂盒
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN110770355A (zh) * 2017-07-31 2020-02-07 凸版印刷株式会社 核酸检测方法及检测用试剂
US11859257B2 (en) 2017-08-11 2024-01-02 Gen-Probe Incorporated Compositions and methods for detecting Staphylococcus aureus
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
US11401546B2 (en) 2017-09-29 2022-08-02 Seegene, Inc. Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
US11662281B2 (en) 2017-12-13 2023-05-30 Gen-Probe Incorporated Compositions and methods for biological sample processing
EP3724354A1 (de) 2017-12-15 2020-10-21 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von toxigenem clostridium difficile
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
EP3914732A1 (de) 2019-01-25 2021-12-01 Gen-Probe Incorporated Nachweis von arzneimittelresistentem mycoplasma genitalium
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021055508A1 (en) 2019-09-16 2021-03-25 Exact Sciences Development Company, Llc Structure and temperature-dependent flap endonuclease substrates
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Crth2のバリアント核酸ライブラリー
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
IL293670A (en) 2019-12-09 2022-08-01 Twist Bioscience Corp Variable nucleic acid libraries for adenosine receptors
US11873485B2 (en) 2021-01-26 2024-01-16 California Institute Of Technology Allosteric conditional guide RNAs for cell-selective regulation of CRISPR/Cas
US20240327899A1 (en) 2021-09-30 2024-10-03 Gen-Probe Incorporated Temperature-selectable fret cassette signaling
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4512922A (en) 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4775619A (en) 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
WO1992006200A1 (en) 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
EP0365627B1 (de) 1988-03-24 1993-12-22 University Of Iowa Research Foundation Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
WO1990010691A1 (en) 1989-03-10 1990-09-20 Grace-Sierra Horticultural Products Company Tissue culturing
US5144019A (en) 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
CA1337639C (en) 1989-08-01 1995-11-28 Joseph Eugene Celebuski Dna probe assay using neutrally charged probe strands
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
DE69032543T2 (de) 1989-12-22 1999-04-15 F. Hoffmann-La Roche Ag, Basel Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6083686A (en) 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5888780A (en) 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5422253A (en) 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
AU693198B2 (en) 1994-04-25 1998-06-25 Avitech Diagnostics, Inc. Detection of mutation by resolvase cleavage
ES2165402T3 (es) 1994-07-16 2002-03-16 Roche Diagnostics Gmbh Procedimiento para la deteccion sensible de acidos nucleicos.
WO1996015267A1 (en) * 1994-11-09 1996-05-23 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants and pathogens
DE4441626A1 (de) 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5545729A (en) 1994-12-22 1996-08-13 Hybridon, Inc. Stabilized ribozyme analogs
WO1996020287A2 (en) 1994-12-23 1996-07-04 Behringwerke Aktiengesellschaft Detection of nucleic acids by target-catalyzed product formation
US5874283A (en) 1995-05-30 1999-02-23 John Joseph Harrington Mammalian flap-specific endonuclease
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
ATE414793T1 (de) * 1996-01-24 2008-12-15 Third Wave Tech Inc Invasive spaltung von nukleinsäuren
WO1998023774A1 (en) * 1996-11-29 1998-06-04 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods

Also Published As

Publication number Publication date
US6555357B1 (en) 2003-04-29
EP0966542B1 (de) 2008-11-12
EP2055781B1 (de) 2011-10-26
EP0966542A4 (de) 2004-04-14
CA2273204A1 (en) 1998-06-04
EP2055781A2 (de) 2009-05-06
HK1163158A1 (en) 2012-09-07
DK0966542T3 (da) 2009-03-23
WO1998023774A1 (en) 1998-06-04
HK1130837A1 (en) 2010-01-08
ES2317658T3 (es) 2009-04-16
EP2055781A3 (de) 2009-10-21
JP2008206521A (ja) 2008-09-11
JP4191228B2 (ja) 2008-12-03
JP4362150B2 (ja) 2009-11-11
EP0966542A1 (de) 1999-12-29
ES2375764T3 (es) 2012-03-06
EP2374890B1 (de) 2012-11-14
JP2001526526A (ja) 2001-12-18
JP2009297032A (ja) 2009-12-24
AU5589898A (en) 1998-06-22
CA2273204C (en) 2008-10-14
JP2007259869A (ja) 2007-10-11
DE69739108D1 (de) 2008-12-24
EP2374890A2 (de) 2011-10-12
EP2374890A3 (de) 2011-11-09
US6562611B1 (en) 2003-05-13
AU737449B2 (en) 2001-08-23
ATE414167T1 (de) 2008-11-15

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