DE69838210D1 - Markierter Primer, geeignet für die Detektion von Nukleinsäuren - Google Patents
Markierter Primer, geeignet für die Detektion von NukleinsäurenInfo
- Publication number
- DE69838210D1 DE69838210D1 DE69838210T DE69838210T DE69838210D1 DE 69838210 D1 DE69838210 D1 DE 69838210D1 DE 69838210 T DE69838210 T DE 69838210T DE 69838210 T DE69838210 T DE 69838210T DE 69838210 D1 DE69838210 D1 DE 69838210D1
- Authority
- DE
- Germany
- Prior art keywords
- labeled
- labeled primer
- primer
- dna sequence
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19755642 | 1997-12-15 | ||
DE19755642A DE19755642B4 (de) | 1997-12-15 | 1997-12-15 | Markierter Primer für die Polymerasekettenreaktion |
DE19806850A DE19806850A1 (de) | 1998-02-19 | 1998-02-19 | Markierter Primer für die Polymerasekettenreaktion |
DE19806850 | 1998-02-19 | ||
DE19834913 | 1998-08-03 | ||
DE19834913A DE19834913A1 (de) | 1998-08-03 | 1998-08-03 | Markierter Primer zum Nachweis amplifizierter Nukleinsäuren |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69838210D1 true DE69838210D1 (de) | 2007-09-20 |
DE69838210T2 DE69838210T2 (de) | 2008-05-15 |
Family
ID=27218019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69838210T Expired - Lifetime DE69838210T2 (de) | 1997-12-15 | 1998-12-10 | Markierter Primer, geeignet für die Detektion von Nukleinsäuren |
Country Status (9)
Country | Link |
---|---|
US (1) | US6248526B1 (de) |
EP (1) | EP0930370B1 (de) |
JP (1) | JP4331812B2 (de) |
KR (1) | KR100702338B1 (de) |
AT (1) | ATE369439T1 (de) |
AU (1) | AU741053B2 (de) |
CA (1) | CA2255531C (de) |
DE (1) | DE69838210T2 (de) |
ES (1) | ES2290979T3 (de) |
Families Citing this family (63)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020188268A1 (en) * | 1999-06-30 | 2002-12-12 | Mark James Kline | Elastomeric side panel for use with convertible absorbent articles |
AU5882000A (en) | 1999-06-22 | 2001-01-09 | Invitrogen Corporation | Improved primers and methods for the detection and discrimination of nucleic acids |
US20070134686A1 (en) * | 1999-10-29 | 2007-06-14 | Stratagene California | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap |
WO2001048246A1 (en) * | 1999-12-29 | 2001-07-05 | Vanderbilt University | Genetic mutation underlying orthostatic intolerance and diagnostic and therapeutic methods relating thereto |
FI20000333A0 (fi) | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
CA2433330A1 (en) * | 2000-12-27 | 2002-07-25 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
KR20020089746A (ko) * | 2001-05-24 | 2002-11-30 | 주식회사 커벡스 | 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법 |
AU2002317973A1 (en) * | 2001-07-20 | 2003-03-03 | Cancer Research Technology Limited | Methods and polynukleotides for assaying the activity of a dna modifying enzyme |
FR2829148B1 (fr) * | 2001-09-06 | 2004-10-15 | Univ Aix Marseille Ii | Identification moleculaire des bacteries du genre staphylococcus |
US20030165859A1 (en) | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
US7163790B2 (en) * | 2001-11-28 | 2007-01-16 | Bio-Rad Laboratories, Inc. | Parallel polymorphism scoring by amplification and error correction |
GB0200526D0 (en) * | 2002-01-11 | 2002-02-27 | Science And Technology The | Methods |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
JP4481656B2 (ja) * | 2002-02-27 | 2010-06-16 | ライフ テクノロジーズ コーポレーション | 3’→5’エキソヌクレアーゼ耐性クエンチャードメインを含むfet標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物 |
CN1414110A (zh) * | 2002-08-19 | 2003-04-30 | 张旭 | 利用具有3'至5'外切酶活性的多聚酶进行基因序列分析 |
BRPI0408967B8 (pt) | 2003-03-31 | 2021-07-27 | Hoffmann La Roche | kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas |
EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
EP2287341B1 (de) | 2003-12-01 | 2013-02-13 | Life Technologies Corporation | Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon |
GB0406015D0 (en) * | 2004-03-17 | 2004-04-21 | Dynal Biotech Asa | Improvements in magnetic polymer particles |
AU2005230833B2 (en) | 2004-04-01 | 2011-04-14 | Bio-Rad Laboratories, Inc. | Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity |
CA2502549C (en) * | 2004-04-23 | 2016-02-16 | Becton, Dickinson And Company | Use of an extraction control in a method of extracting nucleic acids |
US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
US20060275792A1 (en) * | 2004-11-15 | 2006-12-07 | Lee Jun E | Enhancement of nucleic acid amplification using double-stranded DNA binding proteins |
EP1892302A1 (de) * | 2005-06-16 | 2008-02-27 | BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. | Nukleinsäurenachweisverfahren mit direkter erzeugung eines messbaren signals |
JP5220597B2 (ja) | 2005-06-23 | 2013-06-26 | キージーン ナムローゼ フェンノートシャップ | 1つ又は複数の多型性を同定する方法およびその使用方法 |
CA2910861C (en) | 2005-09-29 | 2018-08-07 | Michael Josephus Theresia Van Eijk | High throughput screening of mutagenized populations |
US10316364B2 (en) | 2005-09-29 | 2019-06-11 | Keygene N.V. | Method for identifying the source of an amplicon |
US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
EP3404114B1 (de) | 2005-12-22 | 2021-05-05 | Keygene N.V. | Verfahren zur auf aflp basierenden hochdurchsatz-polymorphismusdetektion |
CA2638854C (en) | 2006-02-27 | 2012-05-15 | F. Hoffmann-La Roche Ag | Pcr hot start by magnesium sequestration |
GB0605584D0 (en) * | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
DK2002017T3 (en) | 2006-04-04 | 2015-09-07 | Keygene Nv | High-capacity detection of molecular markers based on restriction fragments |
CN102046788B (zh) * | 2008-05-28 | 2013-07-10 | 和光纯药工业株式会社 | 胞内分枝杆菌检测用引物和探针、以及使用该引物和探针检测胞内分枝杆菌的方法 |
KR101590175B1 (ko) * | 2009-09-24 | 2016-01-29 | 주식회사 씨젠 | 반복적 엑소핵산 절단 반응에 의한 타겟 핵산서열의 검출 |
JP6147502B2 (ja) * | 2009-10-27 | 2017-06-14 | スウィフト バイオサイエンシーズ, インコーポレイテッド | ポリヌクレオチドプライマー及びプローブ |
KR20110050327A (ko) | 2009-11-07 | 2011-05-13 | 주식회사 씨젠 | Thd 프라이머 타겟 검출 |
CN102762744B (zh) | 2009-12-21 | 2017-07-25 | Seegene株式会社 | 利用靶信号产生引物进行的靶检测 |
GB201004292D0 (en) * | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
US10669569B2 (en) | 2010-10-15 | 2020-06-02 | Navinci Diagnostics Ab | Dynamic range methods |
GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
EP2559774A1 (de) | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes |
GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
US20150232940A1 (en) * | 2012-03-09 | 2015-08-20 | Insight Genetics, Inc | Methods and compositions relating to diagnosing and treating receptor tyrosine kinase related cancers |
CN104919057B (zh) | 2012-11-14 | 2018-09-25 | 欧凌科公司 | 基于rca的局部扩增方法 |
EP2920320B1 (de) | 2012-11-14 | 2016-12-28 | Olink Bioscience AB | Rca-reportersonden und deren verwendung zur detektion von nukleinsäuremolekülen |
US20140341884A1 (en) * | 2012-12-04 | 2014-11-20 | Roche Molecular Systems, Inc. | Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain |
CA2897474A1 (en) | 2013-01-24 | 2014-07-31 | California Institute Of Technology | Chromophore-based characterization and detection methods |
US10113201B2 (en) * | 2013-04-05 | 2018-10-30 | The Wistar Institute Of Anatomy And Biology | Methods and compositions for diagnosis of glioblastoma or a subtype thereof |
GB201320145D0 (en) | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
US20150153346A1 (en) * | 2013-11-15 | 2015-06-04 | The Regents Of The University Of Michigan | Lung cancer signature |
GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
WO2016107833A1 (en) * | 2014-12-31 | 2016-07-07 | F. Hoffmann-La Roche Ag | A novel high-throughput method for quantification of hbv cccdna from cell lysate by real-time pcr |
GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
WO2017132139A1 (en) * | 2016-01-25 | 2017-08-03 | University Of Utah Research Foundation | Methods and compositions for predicting a colon cancer subtype |
GB201621514D0 (en) | 2016-12-16 | 2017-02-01 | Q-Linea Ab | Padlock probe detection method |
WO2019068880A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | RNA MATRIX LIGATION |
US20190112636A1 (en) * | 2017-10-16 | 2019-04-18 | ChromaCode, Inc. | Methods and compositions for nucleic acid detection |
CN109694905A (zh) * | 2019-01-29 | 2019-04-30 | 深圳市亚辉龙生物科技股份有限公司 | 检测待测基因甲基化的核酸组合物及其试剂盒和待测基因甲基化的检测方法 |
GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990011372A1 (en) * | 1989-03-21 | 1990-10-04 | Collaborative Research, Inc. | Multiplex dna diagnostic test |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5804380A (en) * | 1993-11-12 | 1998-09-08 | Geron Corporation | Telomerase activity assays |
US5716784A (en) * | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
-
1998
- 1998-12-10 ES ES98123569T patent/ES2290979T3/es not_active Expired - Lifetime
- 1998-12-10 AT AT98123569T patent/ATE369439T1/de active
- 1998-12-10 DE DE69838210T patent/DE69838210T2/de not_active Expired - Lifetime
- 1998-12-10 EP EP98123569A patent/EP0930370B1/de not_active Expired - Lifetime
- 1998-12-14 AU AU97088/98A patent/AU741053B2/en not_active Ceased
- 1998-12-14 US US09/210,657 patent/US6248526B1/en not_active Expired - Lifetime
- 1998-12-14 CA CA002255531A patent/CA2255531C/en not_active Expired - Fee Related
- 1998-12-15 JP JP35573398A patent/JP4331812B2/ja not_active Expired - Fee Related
- 1998-12-15 KR KR1019980054960A patent/KR100702338B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AU741053B2 (en) | 2001-11-22 |
AU9708898A (en) | 1999-07-01 |
KR100702338B1 (ko) | 2008-03-19 |
JP4331812B2 (ja) | 2009-09-16 |
CA2255531A1 (en) | 1999-06-15 |
ATE369439T1 (de) | 2007-08-15 |
US6248526B1 (en) | 2001-06-19 |
ES2290979T3 (es) | 2008-02-16 |
KR19990063053A (ko) | 1999-07-26 |
CA2255531C (en) | 2007-11-13 |
DE69838210T2 (de) | 2008-05-15 |
EP0930370B1 (de) | 2007-08-08 |
EP0930370A1 (de) | 1999-07-21 |
JPH11313685A (ja) | 1999-11-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |