JP4331812B2 - 標的核酸の検出における標識プライマー - Google Patents
標的核酸の検出における標識プライマー Download PDFInfo
- Publication number
- JP4331812B2 JP4331812B2 JP35573398A JP35573398A JP4331812B2 JP 4331812 B2 JP4331812 B2 JP 4331812B2 JP 35573398 A JP35573398 A JP 35573398A JP 35573398 A JP35573398 A JP 35573398A JP 4331812 B2 JP4331812 B2 JP 4331812B2
- Authority
- JP
- Japan
- Prior art keywords
- primer
- nucleic acid
- label
- target nucleic
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19755642A DE19755642B4 (de) | 1997-12-15 | 1997-12-15 | Markierter Primer für die Polymerasekettenreaktion |
DE19806850A DE19806850A1 (de) | 1998-02-19 | 1998-02-19 | Markierter Primer für die Polymerasekettenreaktion |
DE19806850:6 | 1998-08-03 | ||
DE19834913:0 | 1998-08-03 | ||
DE19834913A DE19834913A1 (de) | 1998-08-03 | 1998-08-03 | Markierter Primer zum Nachweis amplifizierter Nukleinsäuren |
DE19755642:6 | 1998-08-03 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH11313685A JPH11313685A (ja) | 1999-11-16 |
JP4331812B2 true JP4331812B2 (ja) | 2009-09-16 |
Family
ID=27218019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP35573398A Expired - Fee Related JP4331812B2 (ja) | 1997-12-15 | 1998-12-15 | 標的核酸の検出における標識プライマー |
Country Status (9)
Country | Link |
---|---|
US (1) | US6248526B1 (de) |
EP (1) | EP0930370B1 (de) |
JP (1) | JP4331812B2 (de) |
KR (1) | KR100702338B1 (de) |
AT (1) | ATE369439T1 (de) |
AU (1) | AU741053B2 (de) |
CA (1) | CA2255531C (de) |
DE (1) | DE69838210T2 (de) |
ES (1) | ES2290979T3 (de) |
Families Citing this family (63)
Publication number | Priority date | Publication date | Assignee | Title |
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US20020188268A1 (en) * | 1999-06-30 | 2002-12-12 | Mark James Kline | Elastomeric side panel for use with convertible absorbent articles |
NZ516278A (en) | 1999-06-22 | 2004-03-26 | Invitrogen Corp | Improved primers and methods for the detection and discrimination of nucleic acids |
US20070134686A1 (en) * | 1999-10-29 | 2007-06-14 | Stratagene California | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap |
AU2462101A (en) * | 1999-12-29 | 2001-07-09 | Vanderbilt University | Genetic mutation underlying orthostatic intolerance and diagnostic and therapeutic methods relating thereto |
FI20000333A0 (fi) | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
JP2004532615A (ja) * | 2000-12-27 | 2004-10-28 | インヴィトロジェン コーポレーション | 核酸の検出および識別のためのプライマーおよび方法 |
KR20020089746A (ko) * | 2001-05-24 | 2002-11-30 | 주식회사 커벡스 | 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법 |
AU2002317973A1 (en) * | 2001-07-20 | 2003-03-03 | Cancer Research Technology Limited | Methods and polynukleotides for assaying the activity of a dna modifying enzyme |
FR2829148B1 (fr) * | 2001-09-06 | 2004-10-15 | Univ Aix Marseille Ii | Identification moleculaire des bacteries du genre staphylococcus |
US20030165859A1 (en) | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
AU2002351193A1 (en) * | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
GB0200526D0 (en) * | 2002-01-11 | 2002-02-27 | Science And Technology The | Methods |
US6818420B2 (en) * | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
CA2472554A1 (en) * | 2002-02-27 | 2003-09-04 | Biosource International Inc. | Methods of using fet labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same |
CN1414110A (zh) * | 2002-08-19 | 2003-04-30 | 张旭 | 利用具有3'至5'外切酶活性的多聚酶进行基因序列分析 |
KR20070121853A (ko) | 2003-03-31 | 2007-12-27 | 에프. 호프만-라 로슈 아게 | 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법 |
EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
ATE469984T1 (de) | 2003-12-01 | 2010-06-15 | Life Technologies Corp | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
GB0406015D0 (en) * | 2004-03-17 | 2004-04-21 | Dynal Biotech Asa | Improvements in magnetic polymer particles |
US7445898B2 (en) | 2004-04-01 | 2008-11-04 | Bio-Rad Laboratories, Inc. | Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity |
CA2502549C (en) * | 2004-04-23 | 2016-02-16 | Becton, Dickinson And Company | Use of an extraction control in a method of extracting nucleic acids |
US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
US20060275792A1 (en) * | 2004-11-15 | 2006-12-07 | Lee Jun E | Enhancement of nucleic acid amplification using double-stranded DNA binding proteins |
WO2006136621A1 (es) * | 2005-06-16 | 2006-12-28 | Biotools Biotechnological & Medical Laboratories, S.A. | Método de detección de ácidos nucleicos mediante generación directa de una señal medible |
DK2292788T3 (da) | 2005-06-23 | 2012-07-23 | Keygene Nv | Strategier til identifikation og detektion af polymorfismer med højt gennemløb |
US10316364B2 (en) | 2005-09-29 | 2019-06-11 | Keygene N.V. | Method for identifying the source of an amplicon |
EP1929039B2 (de) | 2005-09-29 | 2013-11-20 | Keygene N.V. | Screening mutagenisierter populationen mit hohem durchsatz |
US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
EP3045544A1 (de) | 2005-12-22 | 2016-07-20 | Keygene N.V. | Verfahren zur hochdurchsatz-polymorphismusdetektion basierend auf aflp |
EP1989324B1 (de) | 2006-02-27 | 2011-05-18 | Roche Diagnostics GmbH | Pcr-heissstart durch magnesium-sequestrierung |
GB0605584D0 (en) * | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
WO2007114693A2 (en) | 2006-04-04 | 2007-10-11 | Keygene N.V. | High throughput detection of molecular markers based on aflp and high throughput sequencing |
ES2650066T3 (es) * | 2008-05-28 | 2018-01-16 | Wako Pure Chemical Industries, Ltd. | Cebador y sonda para la detección de Mycobacterium intracellulare, y método para la detección de Mycobacterium intracellulare empleando el cebador o la sonda |
WO2011037306A1 (en) * | 2009-09-24 | 2011-03-31 | Seegene, Inc. | Detection of target nucleic acid sequences by cyclic exonucleolytic reactions |
AU2010315399B2 (en) | 2009-10-27 | 2016-01-28 | Swift Biosciences, Inc. | Bimolecular primers |
KR20110050327A (ko) * | 2009-11-07 | 2011-05-13 | 주식회사 씨젠 | Thd 프라이머 타겟 검출 |
WO2011078441A1 (en) | 2009-12-21 | 2011-06-30 | Seegene, Inc. | Tsg primer target detection |
GB201004292D0 (en) * | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
WO2012049316A1 (en) | 2010-10-15 | 2012-04-19 | Olink Ab | Dynamic range methods |
GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
EP2559774A1 (de) | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes |
GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
WO2013134693A1 (en) * | 2012-03-09 | 2013-09-12 | Insight Genetics, Inc. | Methods and compositions relating to diagnosing and treating receptor tyrosine kinase related cancers |
CN104955963A (zh) | 2012-11-14 | 2015-09-30 | 欧凌科公司 | Rca报告探针及其用于检测核酸分子的用途 |
CN104919057B (zh) | 2012-11-14 | 2018-09-25 | 欧凌科公司 | 基于rca的局部扩增方法 |
US20140341884A1 (en) * | 2012-12-04 | 2014-11-20 | Roche Molecular Systems, Inc. | Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain |
EP2948566A4 (de) | 2013-01-24 | 2016-10-05 | California Inst Of Techn | Chromophorenbasierte charakterisierungs- und erkennungsverfahren |
US10113201B2 (en) * | 2013-04-05 | 2018-10-30 | The Wistar Institute Of Anatomy And Biology | Methods and compositions for diagnosis of glioblastoma or a subtype thereof |
GB201320145D0 (en) | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
US20150153346A1 (en) * | 2013-11-15 | 2015-06-04 | The Regents Of The University Of Michigan | Lung cancer signature |
GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
EP3240913A1 (de) * | 2014-12-31 | 2017-11-08 | F. Hoffmann-La Roche AG | Neuartiges verfahren mit hohem durchsatz zur quantifizierung von hbv cccdna aus zell-lysat mittels echtzeit-pcr |
GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
WO2017132139A1 (en) * | 2016-01-25 | 2017-08-03 | University Of Utah Research Foundation | Methods and compositions for predicting a colon cancer subtype |
GB201621514D0 (en) | 2016-12-16 | 2017-02-01 | Q-Linea Ab | Padlock probe detection method |
US11597965B2 (en) | 2017-10-06 | 2023-03-07 | 10X Genomics, Inc. | RNA templated ligation |
US20190112636A1 (en) * | 2017-10-16 | 2019-04-18 | ChromaCode, Inc. | Methods and compositions for nucleic acid detection |
CN109694905A (zh) * | 2019-01-29 | 2019-04-30 | 深圳市亚辉龙生物科技股份有限公司 | 检测待测基因甲基化的核酸组合物及其试剂盒和待测基因甲基化的检测方法 |
GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU5640090A (en) | 1989-03-21 | 1990-11-05 | Collaborative Research Inc. | A dna diagnostic test using an exonuclease activity |
US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5804380A (en) * | 1993-11-12 | 1998-09-08 | Geron Corporation | Telomerase activity assays |
US5716784A (en) * | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
-
1998
- 1998-12-10 AT AT98123569T patent/ATE369439T1/de active
- 1998-12-10 DE DE69838210T patent/DE69838210T2/de not_active Expired - Lifetime
- 1998-12-10 ES ES98123569T patent/ES2290979T3/es not_active Expired - Lifetime
- 1998-12-10 EP EP98123569A patent/EP0930370B1/de not_active Expired - Lifetime
- 1998-12-14 CA CA002255531A patent/CA2255531C/en not_active Expired - Fee Related
- 1998-12-14 AU AU97088/98A patent/AU741053B2/en not_active Ceased
- 1998-12-14 US US09/210,657 patent/US6248526B1/en not_active Expired - Lifetime
- 1998-12-15 JP JP35573398A patent/JP4331812B2/ja not_active Expired - Fee Related
- 1998-12-15 KR KR1019980054960A patent/KR100702338B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ES2290979T3 (es) | 2008-02-16 |
KR100702338B1 (ko) | 2008-03-19 |
DE69838210T2 (de) | 2008-05-15 |
DE69838210D1 (de) | 2007-09-20 |
AU9708898A (en) | 1999-07-01 |
CA2255531A1 (en) | 1999-06-15 |
CA2255531C (en) | 2007-11-13 |
KR19990063053A (ko) | 1999-07-26 |
EP0930370B1 (de) | 2007-08-08 |
JPH11313685A (ja) | 1999-11-16 |
ATE369439T1 (de) | 2007-08-15 |
EP0930370A1 (de) | 1999-07-21 |
AU741053B2 (en) | 2001-11-22 |
US6248526B1 (en) | 2001-06-19 |
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