DE63763T1 - Plasmide. - Google Patents

Plasmide.

Info

Publication number
DE63763T1
DE63763T1 DE198282103222T DE82103222T DE63763T1 DE 63763 T1 DE63763 T1 DE 63763T1 DE 198282103222 T DE198282103222 T DE 198282103222T DE 82103222 T DE82103222 T DE 82103222T DE 63763 T1 DE63763 T1 DE 63763T1
Authority
DE
Germany
Prior art keywords
plasmid
pcg
microorganism
atcc
corynebacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282103222T
Other languages
English (en)
Inventor
Akira Kawasaki-Shi Kanagawa-Ken Furuya
Ryoichi Machida-Shi Tokyo Katsumata
Tetsuo Yokohama-Shi Kanagawa-Ken Oka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Publication of DE63763T1 publication Critical patent/DE63763T1/de
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15Corynebacterium
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/84Brevibacterium
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/843Corynebacterium

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Claims (10)

Patentansprüche
1. Ein Plasmid, das autonom in einem Mikroorganismus der Gattung Corynebacterium oder Brevibacterium repliziert und das ein Gen für die Resistenz gegenüber mindestens Streptomycin und/oder Spectinomycin trägt.
2. Ein Plasmid nach Anspruch 1, das aus einem Mikroorganismus der Gattung Corynebacterium isoliert ist.
3. Ein Plasmid nach Anspruch 2, wobei der Mikroorganismus Corynebacterium glutamicum ist.
4. Ein Plasmid nach Anspruch 3, wobei der Mikroorganismus Corynebacterium glutamicum 225-250 ist.
5. Plasmid pCG 4, gekennzeichnet durch ein Molekulargewicht von etwa 19 Megadalton, eine Anzahl von Spaltstellen für die Restriktionsendonukleasen Eco RI, Bam HI, Hind III, Pst I und Sal I von 4, 7, 9, 6 bzw. 6, und daö ein Gen für die Resistenz gegenüber mindestens Streptomycin und/oder Spectinomycin trägt.
6. Ein Derivat des Plasmids gemäß Anspruch 1, wobei ein Bereich des Plasmids entfernt und bzw. oder ein anderes DNA-Fragment eingesetzt ist.
7. Ein Derivat nach Anspruch 6, wobei das Plasmid pCG 11 ist.
_2_
8. Eine biologisch reine Kultur des Mikroorganismus Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022, der das Plasmid pCG 11 enthält.
9. Eine biologisch reine Kultur des Mikroorganismus Corynebacterium glutamicum 225-250 mit den identifizierenden Merkmalen von ATCC 31830, der das Plasmid pCG 4 enthält.
10. Eine biologisch reine Kultur eines Mikroorganismus aus der Gruppe Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 und Brevibacterium lactofermentum ATCC 13655/pCG 4 mit den identifizierenden Eigenschaften von ATCC 31837, 31838 bzw. 31839, der das Plasmid pCG 4 enthält.
DE198282103222T 1981-04-17 1982-04-16 Plasmide. Pending DE63763T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56058186A JPS57183799A (en) 1981-04-17 1981-04-17 Novel plasmid

Publications (1)

Publication Number Publication Date
DE63763T1 true DE63763T1 (de) 1983-09-01

Family

ID=13076978

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198282103222T Pending DE63763T1 (de) 1981-04-17 1982-04-16 Plasmide.
DE8282103222T Expired DE3274559D1 (en) 1981-04-17 1982-04-16 Novel plasmids

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8282103222T Expired DE3274559D1 (en) 1981-04-17 1982-04-16 Novel plasmids

Country Status (7)

Country Link
US (1) US4500640A (de)
EP (1) EP0063763B1 (de)
JP (1) JPS57183799A (de)
AU (2) AU564571B2 (de)
CA (1) CA1188236A (de)
DE (2) DE63763T1 (de)
IL (1) IL65571A (de)

Families Citing this family (126)

* Cited by examiner, † Cited by third party
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IL65571A (en) 1985-06-30
EP0063763B1 (de) 1986-12-03
JPS57183799A (en) 1982-11-12
DE3274559D1 (en) 1987-01-15
CA1188236A (en) 1985-06-04
EP0063763A1 (de) 1982-11-03
AU564571B2 (en) 1987-08-20
AU602364B2 (en) 1990-10-11
US4500640A (en) 1985-02-19
AU8268582A (en) 1982-10-21
IL65571A0 (en) 1982-07-30
JPH028714B2 (de) 1990-02-26
AU8063187A (en) 1988-03-10

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