DE64680T1 - Lysozym-sensitiver mikroorganismus. - Google Patents

Lysozym-sensitiver mikroorganismus.

Info

Publication number
DE64680T1
DE64680T1 DE198282103677T DE82103677T DE64680T1 DE 64680 T1 DE64680 T1 DE 64680T1 DE 198282103677 T DE198282103677 T DE 198282103677T DE 82103677 T DE82103677 T DE 82103677T DE 64680 T1 DE64680 T1 DE 64680T1
Authority
DE
Germany
Prior art keywords
microorganism
brevibacterium
corynebacterium
lysozyme
ferm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282103677T
Other languages
English (en)
Inventor
Akira Kawasaki-Shi Kanagawa-Ken Furuya
Ryoichi Machida-Shi Tokyo Katsumata
Tetsuo Yokohama-Shi Kanagawa-Ken Oka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP6577781A external-priority patent/JPS57186489A/ja
Priority claimed from JP56151464A external-priority patent/JPS5856678A/ja
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Publication of DE64680T1 publication Critical patent/DE64680T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/84—Brevibacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/843—Corynebacterium

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (9)

Γ 006468Q EP 82 10 3677.9 Kyowa Hakko Kogyo Co., Ltd u.Z.: R 832 EP Patentansprüche
1. Ein neuer Lysozym-empfindlicher Mikroorganismus der Gattung Corynebacterium oder Brevibacterium mit einer Empfindlichkeit gegenüber Lysozym bei einer Konzentration von weniger als 25 pg/ml.
2. Ein Mikroorganismus nach Anspruch 1, wobei der Mikro-Organismus Corynebacterium glutamicum, Corynebacterium herculis, Brevibacterium divaricatum oder Brevibacterium lactofermentum ist.
3. Ein Mikroorganismus nach Anspruch 2, wobei der Mikro-Organismus Corynebacterium glutamicum L-15 (FERM P-5946,
ATCC 31834), Corynebacterium herculis L-103 (FERM P-5947, ATCC 31866), Brevibacterium divaricatum L-204 (FERM P-5948, ATCC 31867) oder Brevibacterium lactofermentum L-312 (FERM P-5949, ATCC 31868) ist.
30
4. Verfahren zur Herstellung eines Protoplasten eines Mikroorganismus der Gattung Corynebacterium oder Brevibacterium, dadurch gekennzeichnet, daß man einen Mikroorganismus der Gattung Corynebacterium oder Brevibacterium mit einer Empfindlichkeit gegenüber Lysozym bei einer Konzentration von weniger als 25μg/ml mit Lysozym behandelt
L J
und einen Protoplasten des Mikroorganismus bildet.
5. Verfahren zur Transformation eines Mikroorganismus der Gattung Corynebacterium oder Brevibacterium, dadurch gekennzeichnet, daß man einen Mikroorganismus der Gattung Corynebacterium oder Brevibacterium mit einer Empfindlichkeit gegenüber Lysozym bei einer Konzentration von weniger als 25 ug/ml mit Lysozym behandelt, in den erhaltenen Proto plast Desoxyribonucleinsäure einschleust und den Protoplast regeneriert.
6. Verfahren zur Verwendung eines Mikroorganismus der Gattung Corynebacterium oder Brevibacterium mit einer Empfindlichkeit gegenüber Lysozym bei einer Konzentration von weniger als 25 ug/ml als Wirt in der Rekombinant-DNA-Technologie .
7. Ein neuer Lysozym-empfindlicher Mikroorganismus der Gattung Corynebacterium oder Brevibacterium mit einer Empfindlichkeit gegenüber Lysozym bei einer Konzentration von weniger als 25 ug/ml, der ein pCG4 Plasmid enthält.
8. Ein Mikroorganismus nach Anspruch 7, wobei der Mikroorganismus Corynebacterium glutamicum, Corynebacterium herculis, Brevibacterium divaricatum oder Brevibacterium lactofermentum ist.
9. Ein Mikroorganismus nach Anspruch 8, wobei der Mikroorganismus Corynebacterium glutamicum Li5/pCG4 (FERM P-5950, ATCC 31835), Corynebacterium herculis L103/pCG4 (FERM P-5951, ATCC 31869) Brevibacterium divaricatum L204/pCG4 (FERM P-5952, ATCC 31870) oder Brevibacterium lactofermentum L312/ pCG4 (FERM P-5953, ATCC 31871) ist.
L J
DE198282103677T 1981-04-30 1982-04-29 Lysozym-sensitiver mikroorganismus. Pending DE64680T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP6577781A JPS57186489A (en) 1981-04-30 1981-04-30 Novel lysozyme sensitive bacterium
JP56151464A JPS5856678A (ja) 1981-09-25 1981-09-25 新規リゾチ−ム感受性微生物

Publications (1)

Publication Number Publication Date
DE64680T1 true DE64680T1 (de) 1983-09-01

Family

ID=26406923

Family Applications (2)

Application Number Title Priority Date Filing Date
DE8282103677T Expired DE3277306D1 (en) 1981-04-30 1982-04-29 Novel lysozyme-sensitive microorganism
DE198282103677T Pending DE64680T1 (de) 1981-04-30 1982-04-29 Lysozym-sensitiver mikroorganismus.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE8282103677T Expired DE3277306D1 (en) 1981-04-30 1982-04-29 Novel lysozyme-sensitive microorganism

Country Status (6)

Country Link
US (1) US4681847A (de)
EP (1) EP0064680B1 (de)
AU (1) AU553125B2 (de)
CA (1) CA1185197A (de)
DE (2) DE3277306D1 (de)
IL (1) IL65650A0 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4637980A (en) * 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
JPH0783714B2 (ja) * 1983-08-29 1995-09-13 味の素株式会社 発酵法によるｌ―アミノ酸の製造法
EP0155594A3 (de) * 1984-03-06 1987-10-07 Massachusetts Institute Of Technology Klonierte Plasmide von Corynebacterium
JP2967996B2 (ja) 1989-06-06 1999-10-25 協和醗酵工業株式会社 Ｌ―トリプトファンの製造法
EP0469517A3 (en) * 1990-07-30 1992-10-28 Kyowa Hakko Kogyo Co., Ltd. Process for producing l-glutamic acid
JP3078312B2 (ja) * 1990-11-22 2000-08-21 協和醗酵工業株式会社 物質の製造法
ATE368114T1 (de) 1998-09-04 2007-08-15 Kyowa Hakko Kogyo Kk Lysozym-empfindliches c. glutamicum
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
JP3876310B2 (ja) 2002-08-20 2007-01-31 独立行政法人産業技術総合研究所 リゾチーム感受性新規微生物
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
ATE525457T1 (de) * 2004-12-06 2011-10-15 Sage Biosciences Inc Verfahren zur züchtung von bakterien zur abgabe von bioaktiven verbindungen an das intestinum von wiederkäuern

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
JPS55127986A (en) * 1979-03-26 1980-10-03 Agency Of Ind Science & Technol Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall
JPS5642593A (en) * 1979-09-18 1981-04-20 Kyowa Hakko Kogyo Co Ltd Production of l-glutamine
US4346169A (en) * 1980-09-15 1982-08-24 Ajinomoto Company, Incorporated Method for production of L-arginine by fermentation

Also Published As

Publication number Publication date
EP0064680A2 (de) 1982-11-17
US4681847A (en) 1987-07-21
AU553125B2 (en) 1986-07-03
EP0064680A3 (en) 1984-04-04
CA1185197A (en) 1985-04-09
DE3277306D1 (en) 1987-10-22
EP0064680B1 (de) 1987-09-16
AU8312282A (en) 1982-11-04
IL65650A0 (en) 1982-07-30

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