JPS55127986A - Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall - Google Patents

Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall

Info

Publication number
JPS55127986A
JPS55127986A JP3656479A JP3656479A JPS55127986A JP S55127986 A JPS55127986 A JP S55127986A JP 3656479 A JP3656479 A JP 3656479A JP 3656479 A JP3656479 A JP 3656479A JP S55127986 A JPS55127986 A JP S55127986A
Authority
JP
Japan
Prior art keywords
bacteria
culture
antibiotic
cell walls
cell wall
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3656479A
Other languages
Japanese (ja)
Other versions
JPS5654151B2 (en
Inventor
Yasuhiko Komatsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP3656479A priority Critical patent/JPS55127986A/en
Publication of JPS55127986A publication Critical patent/JPS55127986A/en
Publication of JPS5654151B2 publication Critical patent/JPS5654151B2/ja
Granted legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02P—CONTROL OR REGULATION OF ELECTRIC MOTORS, ELECTRIC GENERATORS OR DYNAMO-ELECTRIC CONVERTERS; CONTROLLING TRANSFORMERS, REACTORS OR CHOKE COILS
    • H02P29/00—Arrangements for regulating or controlling electric motors, appropriate for both AC and DC motors
    • H02P29/02—Providing protection against overload without automatic interruption of supply
    • H02P29/024—Detecting a fault condition, e.g. short circuit, locked rotor, open circuit or loss of load
    • H02P29/025—Detecting a fault condition, e.g. short circuit, locked rotor, open circuit or loss of load the fault being a power interruption

Landscapes

  • Engineering & Computer Science (AREA)
  • Power Engineering (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: To form protoplasts even from bacteria having sparingly soluble cell walls effectively, by adding a cell wall synthesis inhibitory antibiotic to a culture of bacteria (B) during incubation as a pretreatment for dissolving B with an enzyme.
CONSTITUTION: In dissolving coryneform bacteria, e.g. glutamic acid producing ones, having sparingly soluble cell walls with an enzyme, an antibiotic e.g. penicillin G (Takeda Yakuhin Kogyo K.K.), Bacitracin, or Griseofulvin, inhibiting the glycopeptide synthesis of the cell walls in an amount of usually 1W50,000 units, or several hundreds mg is added to 100ml of the culture of the bacteria, preferably in the later period of the logarithmic growth. About 30W60min after the addition, bacterium bodies are collected from the culture, and treated with cytolysin by the conventional method.
COPYRIGHT: (C)1980,JPO&Japio
JP3656479A 1979-03-26 1979-03-26 Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall Granted JPS55127986A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3656479A JPS55127986A (en) 1979-03-26 1979-03-26 Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3656479A JPS55127986A (en) 1979-03-26 1979-03-26 Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall

Publications (2)

Publication Number Publication Date
JPS55127986A true JPS55127986A (en) 1980-10-03
JPS5654151B2 JPS5654151B2 (en) 1981-12-23

Family

ID=12473244

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3656479A Granted JPS55127986A (en) 1979-03-26 1979-03-26 Bacteria pretreatment with antibiotic inhibiting synthesis of cell wall

Country Status (1)

Country Link
JP (1) JPS55127986A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0064680A2 (en) * 1981-04-30 1982-11-17 Kyowa Hakko Kogyo Co., Ltd. Novel lysozyme-sensitive microorganism
EP0405858A2 (en) * 1989-06-26 1991-01-02 Eli Lilly And Company Control of aberrant expression vector accumulation during fermentation procedures

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0064680A2 (en) * 1981-04-30 1982-11-17 Kyowa Hakko Kogyo Co., Ltd. Novel lysozyme-sensitive microorganism
EP0405858A2 (en) * 1989-06-26 1991-01-02 Eli Lilly And Company Control of aberrant expression vector accumulation during fermentation procedures

Also Published As

Publication number Publication date
JPS5654151B2 (en) 1981-12-23

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