DE60222914D1 - Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden - Google Patents

Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden

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Publication number
DE60222914D1
DE60222914D1 DE60222914T DE60222914T DE60222914D1 DE 60222914 D1 DE60222914 D1 DE 60222914D1 DE 60222914 T DE60222914 T DE 60222914T DE 60222914 T DE60222914 T DE 60222914T DE 60222914 D1 DE60222914 D1 DE 60222914D1
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Prior art keywords
methods
mutagenesis
oligonucleotides
systems
adjusted
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Expired - Fee Related
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DE60222914T
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English (en)
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DE60222914T2 (de
Inventor
Francesca Storici
Michael A Resnick
Lysle Kevin Lewis
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US Department of Health and Human Services
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US Department of Health and Human Services
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
DE60222914T 2001-07-27 2002-07-26 Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden Expired - Fee Related DE60222914T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30842601P 2001-07-27 2001-07-27
US308426P 2001-07-27
PCT/US2002/023634 WO2003012036A2 (en) 2001-07-27 2002-07-26 Systems for in vivo site-directed mutagenesis using oligonucleotides

Publications (2)

Publication Number Publication Date
DE60222914D1 true DE60222914D1 (de) 2007-11-22
DE60222914T2 DE60222914T2 (de) 2008-07-24

Family

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Family Applications (1)

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DE60222914T Expired - Fee Related DE60222914T2 (de) 2001-07-27 2002-07-26 Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden

Country Status (8)

Country Link
US (1) US7314712B2 (de)
EP (1) EP1421187B1 (de)
JP (1) JP2005500841A (de)
AT (1) ATE375388T1 (de)
AU (1) AU2002319696B2 (de)
CA (1) CA2455013A1 (de)
DE (1) DE60222914T2 (de)
WO (1) WO2003012036A2 (de)

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