EP2732033A1 - Lipasevarianten und dafür kodierende polynucleotide - Google Patents

Lipasevarianten und dafür kodierende polynucleotide

Info

Publication number
EP2732033A1
EP2732033A1 EP12733085.0A EP12733085A EP2732033A1 EP 2732033 A1 EP2732033 A1 EP 2732033A1 EP 12733085 A EP12733085 A EP 12733085A EP 2732033 A1 EP2732033 A1 EP 2732033A1
Authority
EP
European Patent Office
Prior art keywords
variant
seq
substitution
amino acid
amide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12733085.0A
Other languages
English (en)
French (fr)
Inventor
Werner Besenmatter
Allan Svendsen
Julie Bille RANNES
Christian JÄCKEL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to EP12733085.0A priority Critical patent/EP2732033A1/de
Publication of EP2732033A1 publication Critical patent/EP2732033A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
EP12733085.0A 2011-07-15 2012-07-03 Lipasevarianten und dafür kodierende polynucleotide Withdrawn EP2732033A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP12733085.0A EP2732033A1 (de) 2011-07-15 2012-07-03 Lipasevarianten und dafür kodierende polynucleotide

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11174169 2011-07-15
EP11184110 2011-10-06
EP12733085.0A EP2732033A1 (de) 2011-07-15 2012-07-03 Lipasevarianten und dafür kodierende polynucleotide
PCT/EP2012/062896 WO2013010783A1 (en) 2011-07-15 2012-07-03 Lipase variants and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
EP2732033A1 true EP2732033A1 (de) 2014-05-21

Family

ID=46466508

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12733085.0A Withdrawn EP2732033A1 (de) 2011-07-15 2012-07-03 Lipasevarianten und dafür kodierende polynucleotide

Country Status (3)

Country Link
EP (1) EP2732033A1 (de)
CN (1) CN103827298A (de)
WO (1) WO2013010783A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103881992B (zh) * 2012-12-21 2017-04-26 沈阳药科大学 一种脂肪酶突变体及其用途
US10035995B2 (en) 2015-12-07 2018-07-31 Eastman Chemical Company CALB variants
CN109182298B (zh) * 2018-08-14 2021-05-11 浙江工业大学 一种重组脂肪酶突变体、工程菌及应用
CN110358751B (zh) * 2019-07-05 2021-07-02 浙江工业大学 一种重组脂肪酶突变体、编码基因、重组工程菌及应用
CN113564144A (zh) * 2020-04-29 2021-10-29 上海奥博生物医药技术有限公司 脂肪酶突变体及其应用
WO2022168062A1 (en) * 2021-02-08 2022-08-11 Universidade De Coimbra Variants of hyperthermophilic carboxylesterase for polymer synthesis
CN117070496B (zh) * 2023-10-16 2023-12-19 深圳昇瑞生物科技有限公司 一种南极假丝酵母脂肪酶b突变体及其制备方法和应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
PT97110B (pt) 1990-03-23 1998-11-30 Gist Brocades Nv Processo para catalisar reaccoes acelaraveis por enzimas, mediante adicao ao meio reaccional de sementes de plantas transgenicas e para obtencao das referidas sementes
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK0765394T3 (da) 1994-06-03 2001-12-10 Novozymes As Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
ATE375388T1 (de) 2001-07-27 2007-10-15 Us Gov Health & Human Serv Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden
RU2474611C2 (ru) * 2006-11-28 2013-02-10 Новозимс А/С Варианты липолитического фермента
EP2507369A1 (de) * 2009-12-03 2012-10-10 Novozymes A/S Varianten eines polypeptids mit lipolytischer aktivität und verbesserter stabilität

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2013010783A1 *

Also Published As

Publication number Publication date
CN103827298A (zh) 2014-05-28
WO2013010783A1 (en) 2013-01-24

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