DE602006016739D1 - Verfahren zur quantifizierung kleiner rna-moleküle - Google Patents

Verfahren zur quantifizierung kleiner rna-moleküle

Info

Publication number
DE602006016739D1
DE602006016739D1 DE602006016739T DE602006016739T DE602006016739D1 DE 602006016739 D1 DE602006016739 D1 DE 602006016739D1 DE 602006016739 T DE602006016739 T DE 602006016739T DE 602006016739 T DE602006016739 T DE 602006016739T DE 602006016739 D1 DE602006016739 D1 DE 602006016739D1
Authority
DE
Germany
Prior art keywords
molecule
rna molecules
small rna
primer
quantifying small
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
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DE602006016739T
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English (en)
Inventor
Christopher K Raymond
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rosetta Inpharmatics LLC
Original Assignee
Rosetta Inpharmatics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rosetta Inpharmatics LLC filed Critical Rosetta Inpharmatics LLC
Publication of DE602006016739D1 publication Critical patent/DE602006016739D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE602006016739T 2005-01-25 2006-01-25 Verfahren zur quantifizierung kleiner rna-moleküle Active DE602006016739D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64717805P 2005-01-25 2005-01-25
PCT/US2006/002591 WO2006081284A2 (en) 2005-01-25 2006-01-25 Methods for quantitating small rna molecules

Publications (1)

Publication Number Publication Date
DE602006016739D1 true DE602006016739D1 (de) 2010-10-21

Family

ID=36608666

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006016739T Active DE602006016739D1 (de) 2005-01-25 2006-01-25 Verfahren zur quantifizierung kleiner rna-moleküle

Country Status (8)

Country Link
US (1) US20090123912A1 (de)
EP (1) EP1851336B1 (de)
JP (1) JP2008528003A (de)
AT (1) ATE480644T1 (de)
AU (1) AU2006208134A1 (de)
CA (1) CA2595716A1 (de)
DE (1) DE602006016739D1 (de)
WO (1) WO2006081284A2 (de)

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ES2465600T3 (es) 2006-01-05 2014-06-06 The Ohio State University Research Foundation Métodos basados en microARN y composiciones para el diagnóstico y tratamiento de cánceres sólidos del páncreas
WO2007109236A2 (en) 2006-03-20 2007-09-27 The Ohio State University Research Foundation Microrna fingerprints during human megakaryocytopoiesis
CN101657547B (zh) 2006-07-13 2016-04-27 俄亥俄州立大学研究基金会 用于诊断和治疗结肠癌相关疾病的基于微小rna的方法和组合物
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WO2008088858A2 (en) * 2007-01-17 2008-07-24 The Johns Hopkins University Compositions and methods featuring micronas for treating neoplasia
EP2109687B1 (de) * 2007-01-31 2014-06-04 The Ohio State University Research Foundation Micro-rna-basierte verfahren zur behandlung von akuter myeloischer leukämie (aml)
US9096906B2 (en) 2007-03-27 2015-08-04 Rosetta Genomics Ltd. Gene expression signature for classification of tissue of origin of tumor samples
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US20100273172A1 (en) 2007-03-27 2010-10-28 Rosetta Genomics Ltd. Micrornas expression signature for determination of tumors origin
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AU2008262252B2 (en) * 2007-06-08 2013-09-12 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for determining hepatocellular carcinoma subtype and detecting hepatic cancer stem cells
US8053186B2 (en) * 2007-06-15 2011-11-08 The Ohio State University Research Foundation Oncogenic ALL-1 fusion proteins for targeting Drosha-mediated microRNA processing
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US8440636B2 (en) 2007-07-31 2013-05-14 The Board Of Regents, The University Of Texas System Micro-RNA family that modulates fibrosis and uses thereof
ES2627059T3 (es) * 2007-08-03 2017-07-26 The Ohio State University Research Foundation Regiones ultraconservadas que codifican ARNnc
CA2926831A1 (en) * 2007-08-22 2009-02-26 The Ohio State University Research Foundation Methods and compositions for inducing deregulation of epha7 and erk phosphorylation in human acute leukemias
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US8911998B2 (en) 2007-10-26 2014-12-16 The Ohio State University Methods for identifying fragile histidine triad (FHIT) interaction and uses thereof
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CN104031984A (zh) * 2008-02-28 2014-09-10 俄亥俄州立大学研究基金会 用于前列腺相关病症的诊断、预后和治疗的基于微rna的方法和组合物
JP2011517932A (ja) * 2008-02-28 2011-06-23 ジ・オハイオ・ステイト・ユニバーシティ・リサーチ・ファウンデイション ヒト慢性リンパ球性白血病（ｃｃｌ）と関連するマイクロｒｎａシグネチャーおよびその使用
EP2265291B1 (de) 2008-03-17 2016-10-19 The Board of Regents of The University of Texas System Identifizierung von mikro-rna bei der erhaltung und regeneration von neuromuskulären synapsen
US8927207B2 (en) * 2008-06-05 2015-01-06 Research Foundation Of State University Of New York miRNAs as therapeutic targets in cancer
CN102149827B (zh) 2008-06-11 2014-08-20 由卫生与公众服务部代表的美利坚合众国政府 MiR-26家族作为肝细胞癌和对治疗的应答性的预测性标志物的用途
EP2641976A3 (de) 2009-01-16 2014-01-08 Cepheid Verfahren zur Erkennung von Gebärmutterhalskrebs
CN102301011B (zh) * 2009-02-02 2014-05-07 埃克西库恩公司 对小rna进行定量的方法
US9493832B2 (en) 2009-02-02 2016-11-15 Cepheid Methods of detecting sepsis
JP5755569B2 (ja) 2009-02-25 2015-07-29 セファイド 肺癌を検出する方法
JP5960060B2 (ja) 2009-11-23 2016-08-02 ジ・オハイオ・ステート・ユニバーシティ 腫瘍細胞の増殖、遊走および浸潤に影響を与えるために有用な物質および方法
WO2011110644A1 (en) * 2010-03-11 2011-09-15 National University Of Ireland, Galway Detection and quantification of micrornas in the circulation and the use of circulating micrornas as biomarkers for cancer
US8642342B2 (en) 2010-08-19 2014-02-04 Regents Of The University Of Michigan Methods for regulating neural differentiation
WO2012065049A1 (en) 2010-11-12 2012-05-18 The Ohio State University Research Foundation Materials and methods related to microrna-21, mismatch repair, and colorectal cancer
CA2817982C (en) 2010-11-15 2020-06-30 The Regents Of The University Of Michigan Controlled release mucoadhesive systems
CN103561750A (zh) 2011-03-07 2014-02-05 俄亥俄州立大学 通过microRNA-155(miR-155)诱导的增变活性使炎症与癌症关联
US8945829B2 (en) 2011-03-22 2015-02-03 Cornell University Distinguishing benign and malignant indeterminate thyroid lesions
CN104364390B (zh) 2011-10-14 2016-08-24 俄亥俄州立大学 与卵巢癌相关的方法和材料
CN104619353A (zh) 2011-12-13 2015-05-13 俄亥俄州国家创新基金会 与miR-21和miR-29a相关的方法和组合物、外切体抑制和癌症转移
CN104685065B (zh) 2012-01-20 2017-02-22 俄亥俄州立大学 浸润性和预后的乳腺癌生物标志物标签
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EP2971103A1 (de) 2013-03-14 2016-01-20 Cepheid Verfahren für den nachweis von lungenkrebs
CN103361415A (zh) * 2013-04-11 2013-10-23 百瑞德（南京）生物科技有限公司 一种前列腺癌生物标志物miR-379、诊断试剂盒及其应用
WO2016134059A1 (en) * 2015-02-17 2016-08-25 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
CN104877992A (zh) * 2015-03-31 2015-09-02 四川大学华西医院 一种microRNA-30a-5p的检测试剂盒和检测方法
JP6623548B2 (ja) * 2015-05-12 2019-12-25 三菱ケミカル株式会社 膵管内乳頭粘液性腫瘍検出用マーカーとキット
CN105567683A (zh) * 2016-01-13 2016-05-11 深圳市坤健创新药物研究院 Dna探针组合和试剂盒
CN105861730B (zh) * 2016-06-15 2019-09-06 中国水产科学研究院淡水渔业研究中心 一种团头鲂饲料糖利用和代谢调控分子标记miRNA及其应用
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JP7226926B2 (ja) * 2018-05-30 2023-02-21 シスメックス株式会社 ｃＤＮＡの合成方法、標的ＲＮＡの検出方法及び試薬キット

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Also Published As

Publication number Publication date
ATE480644T1 (de) 2010-09-15
CA2595716A1 (en) 2006-08-03
WO2006081284A3 (en) 2007-01-18
EP1851336B1 (de) 2010-09-08
US20090123912A1 (en) 2009-05-14
AU2006208134A1 (en) 2006-08-03
EP1851336A2 (de) 2007-11-07
WO2006081284A2 (en) 2006-08-03
JP2008528003A (ja) 2008-07-31

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