DE602005016220D1 - Aufreinigung von antikörpern - Google Patents

Aufreinigung von antikörpern

Info

Publication number
DE602005016220D1
DE602005016220D1 DE602005016220T DE602005016220T DE602005016220D1 DE 602005016220 D1 DE602005016220 D1 DE 602005016220D1 DE 602005016220 T DE602005016220 T DE 602005016220T DE 602005016220 T DE602005016220 T DE 602005016220T DE 602005016220 D1 DE602005016220 D1 DE 602005016220D1
Authority
DE
Germany
Prior art keywords
cation
aromatic
solution
modal
antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005016220T
Other languages
English (en)
Inventor
Bo-Lennart Johansson
Hans J Johansson
Anders Lungloef
Jean-Luc Maloisel
Nicolas Thevenin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of DE602005016220D1 publication Critical patent/DE602005016220D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/04—Processes using organic exchangers
    • B01J39/07—Processes using organic exchangers in the weakly acidic form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/014—Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DE602005016220T 2004-02-27 2005-02-24 Aufreinigung von antikörpern Active DE602005016220D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0400501A SE0400501D0 (sv) 2004-02-27 2004-02-27 Antibody purification
PCT/SE2005/000292 WO2005082926A1 (en) 2004-02-27 2005-02-24 Antibody purification

Publications (1)

Publication Number Publication Date
DE602005016220D1 true DE602005016220D1 (de) 2009-10-08

Family

ID=32067281

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005016220T Active DE602005016220D1 (de) 2004-02-27 2005-02-24 Aufreinigung von antikörpern

Country Status (13)

Country Link
US (1) US7385040B2 (de)
EP (1) EP1718668B1 (de)
JP (1) JP4776615B2 (de)
KR (1) KR101149969B1 (de)
CN (2) CN1926146B (de)
AT (1) ATE440858T1 (de)
AU (1) AU2005217348B2 (de)
CA (1) CA2552823C (de)
DE (1) DE602005016220D1 (de)
IL (1) IL176635A (de)
NZ (1) NZ548127A (de)
SE (1) SE0400501D0 (de)
WO (1) WO2005082926A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160279239A1 (en) 2011-05-02 2016-09-29 Immunomedics, Inc. Subcutaneous administration of anti-cd74 antibody for systemic lupus erythematosus and autoimmune disease
US20160355591A1 (en) 2011-05-02 2016-12-08 Immunomedics, Inc. Subcutaneous anti-hla-dr monoclonal antibody for treatment of hematologic malignancies
US7589183B2 (en) * 2006-11-28 2009-09-15 Bio-Rad Laboratories, Inc. Prevention of leaching of ligands from affinity-based purification systems
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7999085B2 (en) * 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
EP2099957A1 (de) * 2007-01-10 2009-09-16 GE Healthcare Bio-Sciences AB Multimodale ionenaustausch-chromotographie-harze
AU2008231721B8 (en) 2007-03-28 2013-01-31 Patheon Holdings I B.V. Expanded bed column and disposable chromatography
MX2009012786A (es) * 2007-06-01 2009-12-10 Hoffmann La Roche Purificacion de inmunoglobulina.
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
EP2027875A1 (de) 2007-08-23 2009-02-25 Octapharma AG Verfahren zur Isolierung und Reinigung eines prionenproteinfreien Target-Proteins
JP5778389B2 (ja) * 2007-10-26 2015-09-16 旭化成ケミカルズ株式会社 たんぱく質の精製方法
US8158405B2 (en) * 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8753868B2 (en) 2008-08-04 2014-06-17 General Electric Company Method and system for selective isolation of target biological molecules in a general purpose system
US20100051554A1 (en) * 2008-08-28 2010-03-04 Dong June Ahn Molecular basket coated micro particles
EP2360183B1 (de) * 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antikörperreinigungsverfahren mittels "mixed-mode" chromatography unter verwendung einer arginine-haltigen beladungslösung
CN101797493B (zh) * 2009-02-06 2014-05-21 上海抗体药物国家工程研究中心有限公司 一种在反应釜中制备亲和层析介质的方法
DE102009057993A1 (de) 2009-06-13 2011-01-20 Sartorius Stedim Biotech Gmbh Polysaccharidmatrix mit aufgepfropftem Polymer, Verfahren zu ihrer Herstellung und Verwendung
US9441011B2 (en) 2009-07-03 2016-09-13 Asahi Kasei Chemicals Corporation Method for purification of antibody using porous membrane having amino group and alkyl group both bound to graft chain immobilized on porous substrate
WO2011049798A1 (en) * 2009-10-20 2011-04-28 Merck Sharp & Dohme Corp. Use of mixed mode chromatography for the capture and purification of basic antibody products
US8277649B2 (en) * 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
EP2695889A1 (de) * 2009-12-29 2014-02-12 Dr. Reddy's Laboratories Limited Proteinreinigung durch Ionenaustausch
KR101152036B1 (ko) 2010-04-27 2012-06-08 (주)셀트리온 컬럼 순환 및 시료 연속주입법을 이용한 단백질 ａ 크로마토그래피 정제 방법
KR101871683B1 (ko) 2010-07-30 2018-06-27 이엠디 밀리포어 코포레이션 크로마토그래피 매질 및 방법
CA2831572C (en) 2011-05-02 2019-11-26 Immunomedics, Inc. Ultrafiltration concentration of allotype selected antibodies for small-volume administration
SG10201605397PA (en) 2011-07-01 2016-08-30 Hoffmann La Roche Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides
US20130109807A1 (en) * 2011-10-26 2013-05-02 Bio-Rad Laboratories, Inc. Removal of virucidal agents in mixed mode chromatography
GB201202762D0 (en) 2012-02-17 2012-04-04 Autology Health Ltd High efficiency cell capture
CN103506079B (zh) * 2012-06-19 2016-04-06 汪志友 一种用于分离纯化抗体的介质及其制备方法
JP6335785B2 (ja) * 2012-09-03 2018-05-30 株式会社カネカ ミックスモード抗体アフィニティー分離マトリックスとそれを用いた精製方法および標的分子
AU2013330344B2 (en) 2012-09-17 2018-07-05 W. R. Grace & Co.-Conn. Chromatography media and devices
CN104797332B (zh) 2012-11-13 2018-05-29 通用电气医疗集团生物工艺研发股份公司 多元阴离子交换基质
AU2014223583B2 (en) 2013-02-26 2019-03-28 Merck Millipore Ltd. Mixed-mode chromatography membranes
TWI596107B (zh) 2013-06-25 2017-08-21 卡地拉保健有限公司 單株抗體之新穎純化方法
KR20160054597A (ko) * 2013-09-17 2016-05-16 가부시키가이샤 가네카 신규 항체 정제 방법 및 그로부터 얻어지는 항체, 및 양이온 교환기를 사용한 신규 항체 정제법 및 그로부터 얻어지는 항체
WO2015071913A1 (en) * 2013-11-17 2015-05-21 New Proteintech Inc. Method of preparing chromatographic materials
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
DE102014010353B4 (de) 2014-07-10 2024-09-12 Sartorius Stedim Biotech Gmbh Vorrichtung, Verwendung dieser Vorrichtung und Verfahren für die Stofftrennung mit verbesserter Ausnutzung der Kapazität chromatographischer Medien
US10308678B2 (en) 2014-07-25 2019-06-04 Asahi Kasei Medical Co., Ltd. Cation exchange chromatographic support and method for using same
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
ES2749383T3 (es) 2014-11-06 2020-03-20 Hoffmann La Roche Variantes de la región Fc con unión al FcRn modificada y procedimientos de uso
WO2016093926A1 (en) 2014-12-08 2016-06-16 Emd Millipore Corporation Mixed bed ion exchange adsorber
EP3302784B1 (de) 2015-06-05 2021-10-06 W.R. Grace & Co.-Conn. Adsorbierende klärmittel für bioprozesstechnik und verfahren zur herstellung und verwendung davon
JP2019070528A (ja) * 2016-02-26 2019-05-09 Jsr株式会社 多孔質担体、アフィニティクロマトグラフィー用多孔質担体、標的物の精製方法、及び抗体
JP6846434B2 (ja) * 2016-04-29 2021-03-24 クレオプティクス・アーゲーＣｒｅｏｐｔｉｘ Ａｇ 分子を回収するための方法およびアセンブリ
IL264631B2 (en) 2016-08-16 2024-05-01 Regeneron Pharma A method for quantifying individual antibodies from a mixture
SG11201902667UA (en) 2016-10-25 2019-05-30 Regeneron Pharma Methods and systems for chromatography data analysis
WO2018187074A1 (en) 2017-04-03 2018-10-11 Immunomedics, Inc. Subcutaneous administration of antibody-drug conjugates for cancer therapy
SG11202001564QA (en) 2017-09-19 2020-04-29 Regeneron Pharma Methods of reducing particle formation and compositions formed thereby
WO2019173731A1 (en) * 2018-03-08 2019-09-12 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
EP3816177A4 (de) * 2018-06-29 2021-12-01 Mitsubishi Chemical Corporation Verfahren zur polypeptidisolierung, verfahren zur polypeptidherstellung und vorrichtung zur polypeptidreinigung
US11884698B2 (en) 2018-07-02 2024-01-30 Regeneron Pharmaceuticals, Inc. Systems and methods for preparing a polypeptide from a mixture
JP2022546901A (ja) 2019-09-05 2022-11-10 バイオ－ラッド・ラボラトリーズ・インコーポレーテッド アニオン交換－疎水性混合モードクロマトグラフィー樹脂

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462046C (sv) 1982-08-23 1998-04-27 Pharmacia Biotech Ab Hybrid-DNA-vektor innehållande DNA-sekvens från Staphylococcus aureus, förfarande för dess framställning och bakterie innehållande densamma
US5151350A (en) * 1982-10-27 1992-09-29 Repligen Corporation Cloned genes encoding recombinant protein a
US4983722A (en) 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
EP0921855B1 (de) * 1996-08-30 2003-11-19 Upfront Chromatography A/S Isolierung von immunglobulinen
SE0002688D0 (sv) 2000-07-17 2000-07-17 Amersham Pharm Biotech Ab Adsorption method and ligands
EP1345694B1 (de) * 2000-12-31 2006-08-02 GE Healthcare Bio-Sciences AB Verfahren zur herstellung von zusammensetzungen mit geringer salzkonzentration
US20020110495A1 (en) * 2001-01-05 2002-08-15 Denis Hunt Devices and methods for purification
SE0103084D0 (sv) 2001-09-14 2001-09-14 Amersham Pharm Biotech Ab Generation of ion exchange media
DE10155984A1 (de) 2001-11-15 2003-05-28 Boehringer Ingelheim Pharma Verfahren zur Reduktion des Liganden-leakage von Affinitätschromatographie-Matrices
CA2473144C (en) 2002-02-05 2013-05-28 Genentech, Inc. Protein purification
PT1601697E (pt) * 2003-02-28 2007-09-04 Lonza Biologics Plc Purificação de anticorpos através de proteína a e cromatografia de troca iónica.

Also Published As

Publication number Publication date
EP1718668B1 (de) 2009-08-26
CN1925898B (zh) 2010-09-01
JP2008505851A (ja) 2008-02-28
CN1926146B (zh) 2010-06-16
CN1925898A (zh) 2007-03-07
KR20070001969A (ko) 2007-01-04
CN1926146A (zh) 2007-03-07
KR101149969B1 (ko) 2012-07-05
US20070112178A1 (en) 2007-05-17
CA2552823C (en) 2013-11-19
ATE440858T1 (de) 2009-09-15
JP4776615B2 (ja) 2011-09-21
AU2005217348A1 (en) 2005-09-09
EP1718668A1 (de) 2006-11-08
IL176635A (en) 2011-07-31
IL176635A0 (en) 2006-10-31
CA2552823A1 (en) 2005-09-09
NZ548127A (en) 2009-09-25
US7385040B2 (en) 2008-06-10
SE0400501D0 (sv) 2004-02-27
WO2005082926A1 (en) 2005-09-09
AU2005217348B2 (en) 2011-03-31

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