DK1105508T3 - Fremgangsmåde til oprensning af biomolekyle- eller proteinkomplekser - Google Patents
Fremgangsmåde til oprensning af biomolekyle- eller proteinkomplekserInfo
- Publication number
- DK1105508T3 DK1105508T3 DK99944420T DK99944420T DK1105508T3 DK 1105508 T3 DK1105508 T3 DK 1105508T3 DK 99944420 T DK99944420 T DK 99944420T DK 99944420 T DK99944420 T DK 99944420T DK 1105508 T3 DK1105508 T3 DK 1105508T3
- Authority
- DK
- Denmark
- Prior art keywords
- biomolecule
- protein complexes
- purification
- vectors
- detecting
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98115448 | 1998-08-17 | ||
| PCT/EP1999/006022 WO2000009716A1 (en) | 1998-08-17 | 1999-08-17 | Method for purifying proteins and/or biomolecule or protein complexes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1105508T3 true DK1105508T3 (da) | 2003-01-06 |
Family
ID=8232468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK99944420T DK1105508T3 (da) | 1998-08-17 | 1999-08-17 | Fremgangsmåde til oprensning af biomolekyle- eller proteinkomplekser |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20020061513A1 (da) |
| EP (2) | EP1105508B1 (da) |
| JP (1) | JP3676978B2 (da) |
| AT (1) | ATE223491T1 (da) |
| AU (1) | AU762961B2 (da) |
| CA (1) | CA2340974C (da) |
| DE (2) | DE1105508T1 (da) |
| DK (1) | DK1105508T3 (da) |
| ES (1) | ES2183601T3 (da) |
| PT (1) | PT1105508E (da) |
| WO (1) | WO2000009716A1 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7192596B2 (en) * | 1996-08-23 | 2007-03-20 | The Health Protection Agency Ipsen Limited | Recombinant toxin fragments |
| WO2000009716A1 (en) * | 1998-08-17 | 2000-02-24 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Method for purifying proteins and/or biomolecule or protein complexes |
| FR2830020B1 (fr) * | 2001-09-25 | 2003-12-19 | Univ Victor Segalen Bordeaux 2 | Acide nucleique pour l'obtention d'un polynucleotide codant une proteine de detection et d'isolement d'un ligand "proie" et applications de cet acide nucleique |
| US7825227B2 (en) * | 2002-05-09 | 2010-11-02 | Prolexys Pharmaceuticals, Inc. | Method for purification of a protein complex and identification of its components |
| CA2463301A1 (en) * | 2004-04-22 | 2005-10-22 | Atanas Iliev Lalev | Method for detecting protein interactions - affinity purification with switching the ligand |
| WO2005023858A1 (en) * | 2003-09-05 | 2005-03-17 | Cellzome Ag | Protein complexes associated with app-processing |
| US7238478B2 (en) | 2003-11-13 | 2007-07-03 | Stratagene California | Compositions and methods for protein isolation |
| WO2006070416A1 (en) * | 2004-12-30 | 2006-07-06 | Fondazione Santa Lucia I.R.C.C.S. | An immunoprecipitation-based method to purify and characterise biological macromolecular complexes |
| EP1841874B1 (en) * | 2005-01-27 | 2010-01-06 | Five Prime Therapeutics, Inc. | Leader sequences for directing secretion of polypeptides and methods for production thereof |
| JPWO2010123013A1 (ja) | 2009-04-22 | 2012-10-25 | 国立大学法人大阪大学 | プロテアーゼ認識配列を有するタグペプチドおよびその利用 |
| FR2944799B1 (fr) * | 2009-04-28 | 2011-05-13 | Ecole De Biolog Ind Ebi | Procede de production d'une proteine d'interet grace a une nouvelle proteine de fusion. |
| WO2016145416A2 (en) * | 2015-03-11 | 2016-09-15 | The Broad Institute, Inc. | Proteomic analysis with nucleic acid identifiers |
| WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5821088A (en) * | 1990-05-11 | 1998-10-13 | Siga Pharmaceuticals, Inc. | Use of gram-positive bacteria to express recombinant proteins |
| WO2000009716A1 (en) * | 1998-08-17 | 2000-02-24 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Method for purifying proteins and/or biomolecule or protein complexes |
-
1999
- 1999-08-17 WO PCT/EP1999/006022 patent/WO2000009716A1/en active IP Right Grant
- 1999-08-17 EP EP99944420A patent/EP1105508B1/en not_active Expired - Lifetime
- 1999-08-17 PT PT99944420T patent/PT1105508E/pt unknown
- 1999-08-17 JP JP2000565150A patent/JP3676978B2/ja not_active Expired - Fee Related
- 1999-08-17 AT AT99944420T patent/ATE223491T1/de active
- 1999-08-17 EP EP02004765A patent/EP1231276A1/en not_active Withdrawn
- 1999-08-17 DK DK99944420T patent/DK1105508T3/da active
- 1999-08-17 DE DE1105508T patent/DE1105508T1/de active Pending
- 1999-08-17 AU AU57362/99A patent/AU762961B2/en not_active Ceased
- 1999-08-17 DE DE69902796T patent/DE69902796T2/de not_active Expired - Lifetime
- 1999-08-17 CA CA002340974A patent/CA2340974C/en not_active Expired - Fee Related
- 1999-08-17 ES ES99944420T patent/ES2183601T3/es not_active Expired - Lifetime
-
2001
- 2001-02-16 US US09/785,793 patent/US20020061513A1/en not_active Abandoned
-
2008
- 2008-06-03 US US12/156,626 patent/US20090042216A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU762961B2 (en) | 2003-07-10 |
| EP1105508B1 (en) | 2002-09-04 |
| JP2002522085A (ja) | 2002-07-23 |
| US20020061513A1 (en) | 2002-05-23 |
| DE69902796D1 (de) | 2002-10-10 |
| US20090042216A1 (en) | 2009-02-12 |
| DE69902796T2 (de) | 2003-08-07 |
| CA2340974C (en) | 2010-01-12 |
| EP1105508A1 (en) | 2001-06-13 |
| ES2183601T3 (es) | 2003-03-16 |
| JP3676978B2 (ja) | 2005-07-27 |
| AU5736299A (en) | 2000-03-06 |
| ATE223491T1 (de) | 2002-09-15 |
| CA2340974A1 (en) | 2000-02-24 |
| DE1105508T1 (de) | 2002-02-21 |
| WO2000009716A1 (en) | 2000-02-24 |
| EP1231276A1 (en) | 2002-08-14 |
| PT1105508E (pt) | 2003-01-31 |
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