PT1105508E - Metodo de purificacao de complexos de biomoleculas ou de proteinas - Google Patents

Metodo de purificacao de complexos de biomoleculas ou de proteinas

Info

Publication number
PT1105508E
PT1105508E PT99944420T PT99944420T PT1105508E PT 1105508 E PT1105508 E PT 1105508E PT 99944420 T PT99944420 T PT 99944420T PT 99944420 T PT99944420 T PT 99944420T PT 1105508 E PT1105508 E PT 1105508E
Authority
PT
Portugal
Prior art keywords
protein complexes
biomolecules
purification
biomolecule
vectors
Prior art date
Application number
PT99944420T
Other languages
English (en)
Inventor
Bertrand Seraphin
Guillaume Rigaut
Original Assignee
Europ Lab Molekularbiolog
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europ Lab Molekularbiolog filed Critical Europ Lab Molekularbiolog
Publication of PT1105508E publication Critical patent/PT1105508E/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PT99944420T 1998-08-17 1999-08-17 Metodo de purificacao de complexos de biomoleculas ou de proteinas PT1105508E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98115448 1998-08-17

Publications (1)

Publication Number Publication Date
PT1105508E true PT1105508E (pt) 2003-01-31

Family

ID=8232468

Family Applications (1)

Application Number Title Priority Date Filing Date
PT99944420T PT1105508E (pt) 1998-08-17 1999-08-17 Metodo de purificacao de complexos de biomoleculas ou de proteinas

Country Status (11)

Country Link
US (2) US20020061513A1 (pt)
EP (2) EP1231276A1 (pt)
JP (1) JP3676978B2 (pt)
AT (1) ATE223491T1 (pt)
AU (1) AU762961B2 (pt)
CA (1) CA2340974C (pt)
DE (2) DE69902796T2 (pt)
DK (1) DK1105508T3 (pt)
ES (1) ES2183601T3 (pt)
PT (1) PT1105508E (pt)
WO (1) WO2000009716A1 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192596B2 (en) * 1996-08-23 2007-03-20 The Health Protection Agency Ipsen Limited Recombinant toxin fragments
JP3676978B2 (ja) * 1998-08-17 2005-07-27 ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー(イーエムビーエル) タンパク質および/または生体分子もしくはタンパク質複合体の精製方法
FR2830020B1 (fr) * 2001-09-25 2003-12-19 Univ Victor Segalen Bordeaux 2 Acide nucleique pour l'obtention d'un polynucleotide codant une proteine de detection et d'isolement d'un ligand "proie" et applications de cet acide nucleique
US7825227B2 (en) * 2002-05-09 2010-11-02 Prolexys Pharmaceuticals, Inc. Method for purification of a protein complex and identification of its components
CA2463301A1 (en) * 2004-04-22 2005-10-22 Atanas Iliev Lalev Method for detecting protein interactions - affinity purification with switching the ligand
AU2003298171A1 (en) * 2003-09-05 2005-03-29 Cellzome Ag Protein complexes associated with app-processing
US7238478B2 (en) 2003-11-13 2007-07-03 Stratagene California Compositions and methods for protein isolation
US20080254551A1 (en) * 2004-12-30 2008-10-16 Achsel Tilmann Immunoprecipitaion-Based Method to Purify and Characterise Biological Macromolecular Complexes
CA2589895A1 (en) * 2005-01-27 2006-08-03 Five Prime Therapeutics, Inc. Leader sequences for directing secretion of polypeptides and methods for production thereof
JPWO2010123013A1 (ja) 2009-04-22 2012-10-25 国立大学法人大阪大学 プロテアーゼ認識配列を有するタグペプチドおよびその利用
FR2944799B1 (fr) * 2009-04-28 2011-05-13 Ecole De Biolog Ind Ebi Procede de production d'une proteine d'interet grace a une nouvelle proteine de fusion.
US20180284125A1 (en) * 2015-03-11 2018-10-04 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
CN111902720A (zh) * 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
JP3676978B2 (ja) * 1998-08-17 2005-07-27 ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー(イーエムビーエル) タンパク質および/または生体分子もしくはタンパク質複合体の精製方法

Also Published As

Publication number Publication date
DE69902796T2 (de) 2003-08-07
CA2340974A1 (en) 2000-02-24
EP1105508A1 (en) 2001-06-13
DE69902796D1 (de) 2002-10-10
JP3676978B2 (ja) 2005-07-27
WO2000009716A1 (en) 2000-02-24
EP1231276A1 (en) 2002-08-14
US20020061513A1 (en) 2002-05-23
AU5736299A (en) 2000-03-06
EP1105508B1 (en) 2002-09-04
JP2002522085A (ja) 2002-07-23
US20090042216A1 (en) 2009-02-12
ES2183601T3 (es) 2003-03-16
DE1105508T1 (de) 2002-02-21
CA2340974C (en) 2010-01-12
DK1105508T3 (da) 2003-01-06
AU762961B2 (en) 2003-07-10
ATE223491T1 (de) 2002-09-15

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