EA200702210A1 - Способ очистки белков - Google Patents

Способ очистки белков

Info

Publication number
EA200702210A1
EA200702210A1 EA200702210A EA200702210A EA200702210A1 EA 200702210 A1 EA200702210 A1 EA 200702210A1 EA 200702210 A EA200702210 A EA 200702210A EA 200702210 A EA200702210 A EA 200702210A EA 200702210 A1 EA200702210 A1 EA 200702210A1
Authority
EA
Eurasian Patent Office
Prior art keywords
proteins
cleaning
cleaning proteins
affinity chromatography
filtration step
Prior art date
Application number
EA200702210A
Other languages
English (en)
Other versions
EA013504B1 (ru
Inventor
Алахари Арунакумари
Гизела Мария Маркес Феррейра
Original Assignee
Медарекс, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Медарекс, Инк. filed Critical Медарекс, Инк.
Publication of EA200702210A1 publication Critical patent/EA200702210A1/ru
Publication of EA013504B1 publication Critical patent/EA013504B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

В настоящем изобретении предложены способы очистки белков. В частности, в указанных способах используется процесс двухстадийной неаффинной хроматографии без использования стадии фильтрации с тангенциальным потоком в ходе процесса.
EA200702210A 2005-04-11 2006-03-21 Способ очистки белков EA013504B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67045705P 2005-04-11 2005-04-11
PCT/US2006/010412 WO2006110277A1 (en) 2005-04-11 2006-03-21 Protein purification using hcic amd ion exchange chromatography

Publications (2)

Publication Number Publication Date
EA200702210A1 true EA200702210A1 (ru) 2008-04-28
EA013504B1 EA013504B1 (ru) 2010-06-30

Family

ID=36764783

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200702210A EA013504B1 (ru) 2005-04-11 2006-03-21 Способ очистки белков

Country Status (11)

Country Link
US (3) US8129508B2 (ru)
EP (1) EP1869067A1 (ru)
JP (1) JP2008535913A (ru)
KR (1) KR20080006601A (ru)
CN (1) CN101218247A (ru)
AU (1) AU2006234909A1 (ru)
CA (1) CA2604877A1 (ru)
EA (1) EA013504B1 (ru)
MX (1) MX2007012499A (ru)
NZ (1) NZ562949A (ru)
WO (1) WO2006110277A1 (ru)

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RU2606609C2 (ru) * 2010-10-27 2017-01-10 Филип Моррис Продактс С.А. Способ (варианты) захвата представляющих интерес частиц из смеси

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JP6182210B2 (ja) 2012-06-29 2017-08-16 イー・エム・デイー・ミリポア・コーポレイシヨン タンパク質精製プロセス中のウイルスの不活性化方法
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CN106455621B (zh) * 2014-04-23 2021-07-20 阿雷克森制药公司 蛋清处理
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Publication number Priority date Publication date Assignee Title
RU2606609C2 (ru) * 2010-10-27 2017-01-10 Филип Моррис Продактс С.А. Способ (варианты) захвата представляющих интерес частиц из смеси

Also Published As

Publication number Publication date
US20090105465A1 (en) 2009-04-23
JP2008535913A (ja) 2008-09-04
US20140179904A1 (en) 2014-06-26
AU2006234909A1 (en) 2006-10-19
EP1869067A1 (en) 2007-12-26
US8129508B2 (en) 2012-03-06
US8697847B2 (en) 2014-04-15
WO2006110277A1 (en) 2006-10-19
EA013504B1 (ru) 2010-06-30
CA2604877A1 (en) 2006-10-19
NZ562949A (en) 2009-05-31
CN101218247A (zh) 2008-07-09
US20120165511A1 (en) 2012-06-28
KR20080006601A (ko) 2008-01-16
MX2007012499A (es) 2007-12-06

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Designated state(s): AM AZ BY KZ KG MD TJ TM RU