DE3642829A1 - Resistenzgen gegen phosphinothricin - Google Patents

Resistenzgen gegen phosphinothricin

Info

Publication number
DE3642829A1
DE3642829A1 DE19863642829 DE3642829A DE3642829A1 DE 3642829 A1 DE3642829 A1 DE 3642829A1 DE 19863642829 DE19863642829 DE 19863642829 DE 3642829 A DE3642829 A DE 3642829A DE 3642829 A1 DE3642829 A1 DE 3642829A1
Authority
DE
Germany
Prior art keywords
resistance
ptc
phosphinothricin
resistance gene
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19863642829
Other languages
English (en)
Inventor
Eckhard Strauch
Wolfgang Dr Wohlleben
Walter Arnold
Renate Alijah
Alfred Prof Dr Puehler
Gerhard Dr Woehner
Ruediger Dr Marquardt
Susanne Dr Grabley
Dieter Dr Brauer
Klaus Dr Bartsch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19863628747 external-priority patent/DE3628747A1/de
Application filed by Hoechst AG filed Critical Hoechst AG
Priority to DE19863642829 priority Critical patent/DE3642829A1/de
Priority to DE19873700313 priority patent/DE3700313A1/de
Priority to DE19873701623 priority patent/DE3701623A1/de
Priority to EP87112023A priority patent/EP0257542B1/de
Priority to ES198787112023T priority patent/ES2038631T3/es
Priority to AT87112023T priority patent/ATE75776T1/de
Priority to FI873610A priority patent/FI100251B/fi
Priority to CA000545037A priority patent/CA1337597C/en
Priority to NZ221526A priority patent/NZ221526A/xx
Priority to AU77318/87A priority patent/AU604743B2/en
Priority to DK198704378A priority patent/DK175254B1/da
Priority to IL8360487A priority patent/IL83604A/en
Priority to JP62209123A priority patent/JPH0797994B2/ja
Priority to CN87105764A priority patent/CN1040772C/zh
Priority to HU727/87A priority patent/HU217208B/hu
Publication of DE3642829A1 publication Critical patent/DE3642829A1/de
Priority to JP2049140A priority patent/JP2749424B2/ja
Priority to US07/736,316 priority patent/US5276268A/en
Priority to US07/795,275 priority patent/US5273894A/en
Priority to GR920401371T priority patent/GR3005200T3/el
Priority to JP6249627A priority patent/JPH07147985A/ja
Priority to US08/487,695 priority patent/US5637489A/en
Priority to US08/479,717 priority patent/US5879903A/en
Priority to JP8268910A priority patent/JP2815847B2/ja
Priority to CN98115017A priority patent/CN1124347C/zh
Priority to CL200401481A priority patent/CL2004001481A1/es
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C12P41/007—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures by reactions involving acyl derivatives of racemic amines

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

Die Hauptanmeldung P 36 28 747.4 betrifft ein Resistenzgen gegen Phosphinothricin (PTC), erhältlich aus der Gesamt-DNA von auf Phosphinothricyl-alanyl-alanin (PTT)-Resistenz selektiertem Streptomyces viridochromogenes DSM 40736 durch Schneiden mit BamHI, Klonieren eines 4,0 kb großen Fragments und Selektion auf PTT-Resistenz, sowie die Verwendung dieses Gens zur Herstellung PTC-resistenter Pflanzen, als PTT-Resistenz-Marker in Bakterien und als PTC-Resistenz-Marker in Pflanzenzellen. Das Resistenzgen ist durch eine Restriktionskarte (Fig. 1) charakterisiert, die ein 4 kb großes BamHI-Fragment näher definiert.
Durch Klonieren von Teilbereichen dieses 4 kb-Fragmentes wurde die Lage des Codierbereichs näher eingegrenzt. Hierbei zeigte sich, daß das Resistenzgen auf dem 1,6 kb SstII-SstI-Fragment (Positionen 0,55 bis 2,15 in Fig. 1 der Hauptanmeldung) liegt. Durch Verdauung mit BglII wird das 0,8 kb große Fragment gewonnen, das nach Einbau in ein Plasmid und Transformation von S. lividans PTT-Resistenz vermittelt. Diese Resistenz ist durch die N-Acetylierung von PCT bedingt (Nachweis wie in der Hauptanmeldung angegeben).
Die Sequenzierung nach Maxam und Gilbert des 0,8 kb-Fragments ergibt die DNA-Sequenz I (Anhang). Aus den offenen Leserahmen dieser Sequenz läßt sich die Lage des Resistenzgens ermitteln (ab Position 258). Es zeigt sich, daß das Ende des Gens auf dem nichtsequenzierten Bereich jenseits der BglII-Schnittstelle (Position 1,8 in Fig. 1 der Hauptanmeldung) liegt. Da jedoch das 0,8 kb große BglII-Fragment PTT-Resistenz vermittelt, und zwar auch - nach Einbau in einen E. coli-Vektor unter Vorschaltung eines E. coli-Promotors wie lac - in E. coli, kann der nichtsequenzierte Teil nur wenige Aminosäuren umfassen.
In der DNA-Sequenz I ist die "Shine-Dalgarno-Sequenz" durch Unterstreichen hervorgehoben, ebenso das als Startcodon wirkende GTG. Der maßgebliche Leserahmen ist also in der obersten Zeile ausgedruckt.
Die DNA-Sequenz II zeigt die Restriktionsschnittstellen innerhalb des sequenzierten Gens. Enzyme, die die Sequenz mehr als sechsmal schneiden, sind nicht angegeben.

Claims (4)

1. Resistenzgen gegen Phosphinothricin (PTC), enthaltend die DNA Sequenz I (Anhang) ab Position 258.
2. Verwendung des Gens nach Anspruch 1 zur Herstellung PTC-resistenter Pflanzen.
3. Verwendung des Gens nach Anspruch 1 als Phosphinothricyl-alanyl-alanin (PTT)-Resistenz-Marker in Bakterien.
4. Verwendung des Gens nach Anspruch 1 als PTC-Resistenz-Marker in Pflanzenzellen.
DE19863642829 1986-08-23 1986-12-16 Resistenzgen gegen phosphinothricin Withdrawn DE3642829A1 (de)

Priority Applications (25)

Application Number Priority Date Filing Date Title
DE19863642829 DE3642829A1 (de) 1986-08-23 1986-12-16 Resistenzgen gegen phosphinothricin
DE19873700313 DE3700313A1 (de) 1986-08-23 1987-01-08 Verwendung eines resistenzgens gegen phosphinothricin
DE19873701623 DE3701623A1 (de) 1986-08-23 1987-01-21 In pflanzen wirksames resistenzgen gegen phosphinothricin und seine verwendung
AT87112023T ATE75776T1 (de) 1986-08-23 1987-08-19 Resistenzgen gegen phosphinothricin und seine verwendung.
ES198787112023T ES2038631T3 (es) 1986-08-23 1987-08-19 Procedimiento para la obtencion de un gen de resistencia frente a fosfinotricina (ptc).
EP87112023A EP0257542B1 (de) 1986-08-23 1987-08-19 Resistenzgen gegen Phosphinothricin und seine Verwendung
FI873610A FI100251B (fi) 1986-08-23 1987-08-20 Fosfinotrisiiniresistenssigeeni ja sen käyttö
CA000545037A CA1337597C (en) 1986-08-23 1987-08-21 Phosphinotricin-resistance gene, and its use
NZ221526A NZ221526A (en) 1986-08-23 1987-08-21 Phosphinothricin resistance gene, cloning and marker
AU77318/87A AU604743B2 (en) 1986-08-23 1987-08-21 Phosphinothricin-resistance gene, and its use
DK198704378A DK175254B1 (da) 1986-08-23 1987-08-21 Resistensgensekvens mod phosphinothricin og anvendelse deraf
IL8360487A IL83604A (en) 1986-08-23 1987-08-21 Phosphinothricin resistance gene obtainable from streptomyces viridochromogenes and its use
CN87105764A CN1040772C (zh) 1986-08-23 1987-08-22 获得2－氨基－4－甲膦酰丁酸基因的方法
JP62209123A JPH0797994B2 (ja) 1986-08-23 1987-08-22 ホスフィノトリシン耐性遺伝子及びその使用
HU727/87A HU217208B (hu) 1986-08-23 1987-08-23 Eljárás foszfinotricinnel szemben rezisztens gén előállítása
JP2049140A JP2749424B2 (ja) 1986-08-23 1990-02-28 ホスフィノトリシン耐性遺伝子の使用
US07/736,316 US5276268A (en) 1986-08-23 1991-07-29 Phosphinothricin-resistance gene, and its use
US07/795,275 US5273894A (en) 1986-08-23 1991-11-20 Phosphinothricin-resistance gene, and its use
GR920401371T GR3005200T3 (de) 1986-08-23 1992-07-16
JP6249627A JPH07147985A (ja) 1986-08-23 1994-10-14 ホスフィノトリシン耐性遺伝子の使用
US08/487,695 US5637489A (en) 1986-08-23 1995-06-07 Phosphinothricin-resistance gene, and its use
US08/479,717 US5879903A (en) 1986-08-23 1995-06-07 Phosphinothricin-resistance gene, and its use
JP8268910A JP2815847B2 (ja) 1986-08-23 1996-10-09 ホスフィノトリシン耐性遺伝子の使用
CN98115017A CN1124347C (zh) 1986-08-23 1998-06-19 2－氨基－4－甲膦酰丁酸抗性基因
CL200401481A CL2004001481A1 (es) 1986-08-23 2004-06-15 Gen de resistencia empleado en plantas para generar resistencia a fosfinotricina, estructura genica y vector que posee el gen de resistencia, celulas vegetales transformadas con el gen de resistencia y procediientos para generar celulas y plantas y s

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19863628747 DE3628747A1 (de) 1986-08-23 1986-08-23 Resistenzgen gegen phosphinothricin und seine verwendung
DE3637307 1986-11-03
DE19863642829 DE3642829A1 (de) 1986-08-23 1986-12-16 Resistenzgen gegen phosphinothricin

Publications (1)

Publication Number Publication Date
DE3642829A1 true DE3642829A1 (de) 1988-05-05

Family

ID=27194757

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19863642829 Withdrawn DE3642829A1 (de) 1986-08-23 1986-12-16 Resistenzgen gegen phosphinothricin

Country Status (2)

Country Link
CL (1) CL2004001481A1 (de)
DE (1) DE3642829A1 (de)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3923085A1 (de) * 1988-07-27 1990-02-01 Hoechst Ag In pflanzen wirksames resistenzgen gegen phosphinothricin und seine verwendung
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
WO2003099216A2 (en) 2002-05-22 2003-12-04 Monsanto Technology Llc Fatty acid desaturases from fungi
EP2123764A1 (de) 1999-05-14 2009-11-25 Dekalb Genetics Corporation Reis-Aktin-2-Promoter und -Intron und Verwendungsverfahren dafür
WO2011050271A1 (en) 2009-10-23 2011-04-28 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
WO2011146754A1 (en) 2010-05-19 2011-11-24 The Samuel Roberts Noble Foundation, Inc. Altered leaf morphology and enhanced agronomic properties in plants
WO2012061615A1 (en) 2010-11-03 2012-05-10 The Samuel Roberts Noble Foundation, Inc. Transcription factors for modification of lignin content in plants
WO2014047653A2 (en) 2012-09-24 2014-03-27 Seminis Vegetable Seeds, Inc. Methods and compositions for extending shelf life of plant products
WO2016077624A1 (en) 2014-11-12 2016-05-19 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
WO2022261067A1 (en) 2021-06-09 2022-12-15 Morehouse School Of Medicine Antimicrobial hevamine a-related compositions and methods

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3923085A1 (de) * 1988-07-27 1990-02-01 Hoechst Ag In pflanzen wirksames resistenzgen gegen phosphinothricin und seine verwendung
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
EP2123764A1 (de) 1999-05-14 2009-11-25 Dekalb Genetics Corporation Reis-Aktin-2-Promoter und -Intron und Verwendungsverfahren dafür
WO2003099216A2 (en) 2002-05-22 2003-12-04 Monsanto Technology Llc Fatty acid desaturases from fungi
WO2011050271A1 (en) 2009-10-23 2011-04-28 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
WO2011146754A1 (en) 2010-05-19 2011-11-24 The Samuel Roberts Noble Foundation, Inc. Altered leaf morphology and enhanced agronomic properties in plants
WO2012061615A1 (en) 2010-11-03 2012-05-10 The Samuel Roberts Noble Foundation, Inc. Transcription factors for modification of lignin content in plants
WO2014047653A2 (en) 2012-09-24 2014-03-27 Seminis Vegetable Seeds, Inc. Methods and compositions for extending shelf life of plant products
EP3622810A1 (de) 2012-09-24 2020-03-18 Seminis Vegetable Seeds, Inc. Verfahren und zusammensetzungen zur verlängerung der haltbarkeit von pflanzlichen produkten
WO2016077624A1 (en) 2014-11-12 2016-05-19 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
US10745708B2 (en) 2014-11-12 2020-08-18 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
US11111497B2 (en) 2014-11-12 2021-09-07 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
WO2022261067A1 (en) 2021-06-09 2022-12-15 Morehouse School Of Medicine Antimicrobial hevamine a-related compositions and methods

Also Published As

Publication number Publication date
CL2004001481A1 (es) 2005-06-03

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