DE2858792C2 - - Google Patents
Info
- Publication number
- DE2858792C2 DE2858792C2 DE2858792A DE2858792A DE2858792C2 DE 2858792 C2 DE2858792 C2 DE 2858792C2 DE 2858792 A DE2858792 A DE 2858792A DE 2858792 A DE2858792 A DE 2858792A DE 2858792 C2 DE2858792 C2 DE 2858792C2
- Authority
- DE
- Germany
- Prior art keywords
- antibody
- antigen
- reaction
- luminescent
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000427 antigen Substances 0.000 claims description 50
- 102000036639 antigens Human genes 0.000 claims description 50
- 108091007433 antigens Proteins 0.000 claims description 50
- 238000006243 chemical reaction Methods 0.000 claims description 50
- 238000004020 luminiscence type Methods 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 24
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 9
- -1 Trÿodthyronine Chemical compound 0.000 claims description 8
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 claims description 7
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 claims description 7
- 229940095074 cyclic amp Drugs 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 102000003992 Peroxidases Human genes 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 6
- 230000001900 immune effect Effects 0.000 claims description 6
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims description 4
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 claims description 4
- 125000004122 cyclic group Chemical group 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 4
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 4
- BQJCRHHNABKAKU-KBQPJGBKSA-N morphine Chemical compound O([C@H]1[C@H](C=C[C@H]23)O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4O BQJCRHHNABKAKU-KBQPJGBKSA-N 0.000 claims description 4
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 4
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
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- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 claims description 3
- PROQIPRRNZUXQM-UHFFFAOYSA-N (16alpha,17betaOH)-Estra-1,3,5(10)-triene-3,16,17-triol Natural products OC1=CC=C2C3CCC(C)(C(C(O)C4)O)C4C3CCC2=C1 PROQIPRRNZUXQM-UHFFFAOYSA-N 0.000 claims description 2
- GMRQFYUYWCNGIN-UHFFFAOYSA-N 1,25-Dihydroxy-vitamin D3' Natural products C1CCC2(C)C(C(CCCC(C)(C)O)C)CCC2C1=CC=C1CC(O)CC(O)C1=C GMRQFYUYWCNGIN-UHFFFAOYSA-N 0.000 claims description 2
- GMRQFYUYWCNGIN-ZVUFCXRFSA-N 1,25-dihydroxy vitamin D3 Chemical compound C1([C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](CCCC(C)(C)O)C)=CC=C1C[C@@H](O)C[C@H](O)C1=C GMRQFYUYWCNGIN-ZVUFCXRFSA-N 0.000 claims description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims description 2
- JWUBBDSIWDLEOM-UHFFFAOYSA-N 25-Hydroxycholecalciferol Natural products C1CCC2(C)C(C(CCCC(C)(C)O)C)CCC2C1=CC=C1CC(O)CCC1=C JWUBBDSIWDLEOM-UHFFFAOYSA-N 0.000 claims description 2
- 239000003872 25-hydroxy-cholecalciferol Substances 0.000 claims description 2
- WCPTXJJVVDAEMW-XVFCMESISA-N 3',5'-cyclic CMP Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H]2OP(O)(=O)OC[C@H]2O1 WCPTXJJVVDAEMW-XVFCMESISA-N 0.000 claims description 2
- ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 claims description 2
- PQSUYGKTWSAVDQ-ZVIOFETBSA-N Aldosterone Chemical compound C([C@@]1([C@@H](C(=O)CO)CC[C@H]1[C@@H]1CC2)C=O)[C@H](O)[C@@H]1[C@]1(C)C2=CC(=O)CC1 PQSUYGKTWSAVDQ-ZVIOFETBSA-N 0.000 claims description 2
- PQSUYGKTWSAVDQ-UHFFFAOYSA-N Aldosterone Natural products C1CC2C3CCC(C(=O)CO)C3(C=O)CC(O)C2C2(C)C1=CC(=O)CC2 PQSUYGKTWSAVDQ-UHFFFAOYSA-N 0.000 claims description 2
- 235000021318 Calcifediol Nutrition 0.000 claims description 2
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 claims description 2
- GVGLGOZIDCSQPN-PVHGPHFFSA-N Heroin Chemical compound O([C@H]1[C@H](C=C[C@H]23)OC(C)=O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4OC(C)=O GVGLGOZIDCSQPN-PVHGPHFFSA-N 0.000 claims description 2
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 claims description 2
- CXOFVDLJLONNDW-UHFFFAOYSA-N Phenytoin Chemical compound N1C(=O)NC(=O)C1(C=1C=CC=CC=1)C1=CC=CC=C1 CXOFVDLJLONNDW-UHFFFAOYSA-N 0.000 claims description 2
- 229960002478 aldosterone Drugs 0.000 claims description 2
- 229940125717 barbiturate Drugs 0.000 claims description 2
- JWUBBDSIWDLEOM-DTOXIADCSA-N calcidiol Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](CCCC(C)(C)O)C)=C\C=C1\C[C@@H](O)CCC1=C JWUBBDSIWDLEOM-DTOXIADCSA-N 0.000 claims description 2
- ZOOGRGPOEVQQDX-UHFFFAOYSA-N cyclic GMP Natural products O1C2COP(O)(=O)OC2C(O)C1N1C=NC2=C1NC(N)=NC2=O ZOOGRGPOEVQQDX-UHFFFAOYSA-N 0.000 claims description 2
- 229960002069 diamorphine Drugs 0.000 claims description 2
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 claims description 2
- 229960005156 digoxin Drugs 0.000 claims description 2
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 claims description 2
- 229930182833 estradiol Natural products 0.000 claims description 2
- PROQIPRRNZUXQM-ZXXIGWHRSA-N estriol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H]([C@H](O)C4)O)[C@@H]4[C@@H]3CCC2=C1 PROQIPRRNZUXQM-ZXXIGWHRSA-N 0.000 claims description 2
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- 239000000186 progesterone Substances 0.000 claims description 2
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- WBWWGRHZICKQGZ-UHFFFAOYSA-N Taurocholic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(=O)NCCS(O)(=O)=O)C)C1(C)C(O)C2 WBWWGRHZICKQGZ-UHFFFAOYSA-N 0.000 claims 1
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- 229960005309 estradiol Drugs 0.000 claims 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 claims 1
- 229960001860 salicylate Drugs 0.000 claims 1
- WBWWGRHZICKQGZ-GIHLXUJPSA-N taurocholic acid Chemical compound C([C@@H]1C[C@H]2O)[C@@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@@H]([C@@H](CCC(=O)NCCS(O)(=O)=O)C)[C@@]2(C)[C@H](O)C1 WBWWGRHZICKQGZ-GIHLXUJPSA-N 0.000 claims 1
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Classifications
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Inorganic Chemistry (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
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| GB4783977 | 1977-11-17 |
Publications (1)
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| DE2858792C2 true DE2858792C2 (en, 2012) | 1991-08-22 |
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| DE19782849708 Granted DE2849708A1 (de) | 1977-11-17 | 1978-11-16 | Verfahren zur feststellung und quantitativen bestimmung biologisch wichtiger substanzen |
| DE2858792A Expired - Lifetime DE2858792C2 (en, 2012) | 1977-11-17 | 1978-11-16 |
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| DE19782849708 Granted DE2849708A1 (de) | 1977-11-17 | 1978-11-16 | Verfahren zur feststellung und quantitativen bestimmung biologisch wichtiger substanzen |
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| CA (1) | CA1113392A (en, 2012) |
| CH (2) | CH640061A5 (en, 2012) |
| DE (2) | DE2849708A1 (en, 2012) |
| FR (1) | FR2409516B1 (en, 2012) |
| GB (1) | GB2095830B (en, 2012) |
| SE (1) | SE7811630L (en, 2012) |
Families Citing this family (47)
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| AU533026B2 (en) * | 1979-10-26 | 1983-10-27 | Dynasciences Corp. | Passively adsorbing immuno-reactive haptens to solid phases |
| DE2947459C2 (de) * | 1979-11-24 | 1983-06-23 | Battelle-Institut E.V., 6000 Frankfurt | Vorrichtung zum in-situ-Nachweis von in Bodenbelägen vermuteten giftigen Substanzen |
| ATE8130T1 (de) * | 1980-08-25 | 1984-07-15 | Kabivitrum Ab | Peptidsubstrate zum bestimmen der proteaseaktivitaet. |
| GB2112779B (en) * | 1981-12-11 | 1986-10-15 | Welsh Nat School Med | Aryl acridinium esters as luminescent labelling materials |
| JPS59500252A (ja) * | 1982-03-03 | 1984-02-23 | ブリティッシュ・テクノロジー・グループ・リミテッド | 増強ルミネツセンスおよび発光分析 |
| ES8607563A1 (es) * | 1983-10-13 | 1986-06-01 | Univ Georgia Res Found | Un metodo para detectar una interaccion ligante |
| US4647531A (en) * | 1984-02-06 | 1987-03-03 | Ortho Diagnostic Systems, Inc. | Generalized cytometry instrument and methods of use |
| ATE56096T1 (de) * | 1984-03-15 | 1990-09-15 | Immunex Corp | Test zur sofortigen feststellung von liganden, testsatz und seine herstellung. |
| FR2562252B1 (fr) * | 1984-03-27 | 1988-01-22 | Inst Nat Sante Rech Med | Procede de dosage immunoenzymatique sans etape de separation et reactifs et necessaires pour sa mise en oeuvre |
| US4977080A (en) * | 1984-04-06 | 1990-12-11 | Minnesota Mining And Manufacturing Co. | Chemiluminescent methods and a kit therefor involving a beta-lactam |
| EP0165072A3 (en) * | 1984-06-15 | 1986-12-30 | Mast Immunosystems, Inc. | Specific binding assay reagent medium, test kit and process |
| US4687747A (en) * | 1984-07-02 | 1987-08-18 | Mallinckrodt, Inc. | Phenanthridinium ester as a labelling compound in luminometric immunoassay |
| US5422266A (en) * | 1984-12-31 | 1995-06-06 | University Of Georgia Research Foundation, Inc. | Recombinant DNA vectors capable of expressing apoaequorin |
| US5155216A (en) * | 1985-08-15 | 1992-10-13 | Amoco Corporation | Nucleic acids labeled with a chemiluminescent acridine ester |
| DE3537877A1 (de) * | 1985-10-24 | 1987-04-30 | Geiger Reinhard | Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate |
| DE3545398A1 (de) * | 1985-12-20 | 1987-06-25 | Boehringer Mannheim Gmbh | Verfahren zur steigerung der quanten-ausbeute bei der oxidation von luminol durch peroxide in gegenwart von peroxidase |
| CA1293725C (en) * | 1986-05-08 | 1991-12-31 | Universite Laval | Luminescent cyclic hydrazides for analytical assays |
| US4777128A (en) * | 1986-05-27 | 1988-10-11 | Ethigen Corporation | Fluorescence immunoassay involving energy transfer between two fluorophores |
| FR2602592B1 (fr) | 1986-08-06 | 1989-06-30 | Alain Baret | Utilisation du systeme enzymatique xanthine-oxydase en immuno-analyse, procedes de dosage correspondants et coffrets de reactifs necessaires pour la mise en oeuvre de ces procedes. |
| FR2617974B1 (fr) * | 1987-07-07 | 1992-11-13 | Stabiligen | Procede de dosage immunologique par bio- ou chimio- luminescence |
| DE3737649A1 (de) * | 1987-11-06 | 1989-05-24 | Inst Zellforschung Und Biolumi | Verfahren zur bestimmung der lumineszenz von zellkulturen sowie vorrichtung zur durchfuehrung des verfahrens |
| JPH01219563A (ja) * | 1988-02-27 | 1989-09-01 | Doujin Kagaku Kenkyusho:Kk | 化学発光の写真法による検出方法 |
| IL92124A (en) | 1988-10-28 | 1996-10-31 | Sidney Pestka | Recombinant proteins modified to contain post-phosphorylation that do not occur in nature |
| US6747131B1 (en) | 1988-10-28 | 2004-06-08 | Pestka Biomedical Laboratories, Inc. | Phosphorylated fusion proteins |
| US5986061A (en) * | 1988-10-28 | 1999-11-16 | Pbl Biomedical Laboratories | Phosphorylated fusion proteins |
| JP3184894B2 (ja) * | 1989-10-05 | 2001-07-09 | エックスオックスエミス， インコーポレイテッド | ハロペルオキシターゼ酸至適化学的発光分析系 |
| US5108899A (en) * | 1989-10-31 | 1992-04-28 | Exoxemis, Inc. | Chemiluminescence assay of in vivo inflammation |
| US5168047A (en) * | 1989-12-04 | 1992-12-01 | Takeda Chemical Industries, Inc. | Method for improvement of sensitivity of enzyme detection by photo irradiation |
| EP0476556A3 (en) * | 1990-09-18 | 1992-11-19 | Takeda Chemical Industries, Ltd. | Method for determination of a light sensitive substance |
| DE4224738A1 (de) * | 1992-07-27 | 1994-02-03 | Engler Blum Gabriele | Verfahren zur Analyse molekularer organischer Strukturen |
| JP3207933B2 (ja) * | 1992-09-09 | 2001-09-10 | 株式会社ヤトロン | アクリジニウム誘導体の発光方法及びそれを用いた検査対象物質の検出方法 |
| ATE148237T1 (de) * | 1993-03-11 | 1997-02-15 | Packard Instr Bv | Szintillationszählmedium und verfahren |
| EP0650044A3 (en) * | 1993-04-02 | 1996-06-19 | Hitachi Chemical Co Ltd | Analytical chemiluminescence method. |
| US5512753A (en) | 1994-06-08 | 1996-04-30 | Packard Instrument, B.V. | Scintillation counting system using scintillator capsules |
| CN1201150C (zh) * | 1994-06-24 | 2005-05-11 | 乔治·W·卡特西罗米特斯 | 一种组合物,化学发光体系及其应用 |
| US7255851B2 (en) * | 1994-07-01 | 2007-08-14 | The Board Of Trustees Of The Leland Stanford Junior University | Non-invasive localization of a light-emitting conjugate in a mammal |
| US6800747B1 (en) | 1995-06-07 | 2004-10-05 | Pestka Biomedical Laboratories, Inc. | Nucleic acids encoding phosphorylated fusion proteins |
| DE19623814C2 (de) * | 1995-06-15 | 1999-02-11 | Lab Molecular Biophotonics | Verfahren und Vorrichtung zur analytischen Bestimmung von 5-Hydroxyindolen oder Catecholaminen |
| US6416960B1 (en) | 1996-08-08 | 2002-07-09 | Prolume, Ltd. | Detection and visualization of neoplastic tissues and other tissues |
| US5902727A (en) * | 1996-09-04 | 1999-05-11 | Washington University | Method for localization and quantitation of a substance in a biological sample |
| US5795784A (en) | 1996-09-19 | 1998-08-18 | Abbott Laboratories | Method of performing a process for determining an item of interest in a sample |
| US5856194A (en) | 1996-09-19 | 1999-01-05 | Abbott Laboratories | Method for determination of item of interest in a sample |
| CA2271717A1 (en) | 1996-12-12 | 1998-06-18 | Prolume, Ltd. | Apparatus and method for detecting and identifying infectious agents |
| US6232107B1 (en) | 1998-03-27 | 2001-05-15 | Bruce J. Bryan | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
| US7109315B2 (en) | 2000-03-15 | 2006-09-19 | Bruce J. Bryan | Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
| US7635571B2 (en) * | 2000-12-07 | 2009-12-22 | Siemens Healthcare Diagnostics Products Gmbh | Amplified signal in binding assays |
| WO2002078906A2 (en) * | 2001-03-29 | 2002-10-10 | Cellect Technologies Corp. | Methods devices and systems for sorting and separating particles |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2618419A1 (de) * | 1975-04-28 | 1976-11-04 | Miles Lab | Heterogenes spezifisches bindungsverfahren zur bestimmung eines liganden in einem fluessigen medium und mittel zu dessen durchfuehrung |
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| US3366572A (en) * | 1965-03-09 | 1968-01-30 | American Cyanamid Co | Oxidation of chemiluminescent substances |
| CA1025772A (en) * | 1972-06-26 | 1978-02-07 | Ivar Giaever | Method and apparatus for detection and purification of proteins and antibodies |
| US4000252A (en) * | 1974-01-04 | 1976-12-28 | Kenneth Kosak | Immunoscintillation cell |
| US3992631A (en) * | 1975-02-27 | 1976-11-16 | International Diagnostic Technology, Inc. | Fluorometric system, method and test article |
| US4011219A (en) * | 1975-03-28 | 1977-03-08 | Chugai Seiyaku Kabushiki Kaisha | Phthalazine derivatives and salts thereof |
| US4041146A (en) * | 1975-05-01 | 1977-08-09 | General Electric Company | Method for detection of biological particles |
| US4181650A (en) * | 1975-08-25 | 1980-01-01 | Maier Charles L Jr | Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids |
| US4036946A (en) * | 1975-10-20 | 1977-07-19 | Marcos Kleinerman | Immunofluorometric method for measuring minute quantities of antigens, antibodies and other substances |
| US4020151A (en) * | 1976-02-17 | 1977-04-26 | International Diagnostic Technology, Inc. | Method for quantitation of antigens or antibodies on a solid surface |
| US4128628A (en) * | 1976-03-12 | 1978-12-05 | University Of Virginia Alumni Patents Foundation | Automated immunoassay |
| US4025310A (en) * | 1976-05-28 | 1977-05-24 | International Diagnostic Technology, Inc. | Method for reading a wet fluorescent surface |
| GB1578275A (en) * | 1977-06-14 | 1980-11-05 | Maier C | Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids |
| US4208479A (en) * | 1977-07-14 | 1980-06-17 | Syva Company | Label modified immunoassays |
| US4160645A (en) * | 1977-07-14 | 1979-07-10 | Syva Company | Catalyst mediated competitive protein binding assay |
-
1978
- 1978-11-10 SE SE7811630A patent/SE7811630L/xx unknown
- 1978-11-13 CH CH1163678A patent/CH640061A5/fr not_active IP Right Cessation
- 1978-11-14 GB GB8133207A patent/GB2095830B/en not_active Expired
- 1978-11-14 US US05/961,389 patent/US4478817A/en not_active Expired - Lifetime
- 1978-11-16 CA CA316,349A patent/CA1113392A/en not_active Expired
- 1978-11-16 DE DE19782849708 patent/DE2849708A1/de active Granted
- 1978-11-16 DE DE2858792A patent/DE2858792C2/de not_active Expired - Lifetime
- 1978-11-17 FR FR7832459A patent/FR2409516B1/fr not_active Expired
-
1983
- 1983-12-14 CH CH667283A patent/CH645725A5/fr not_active IP Right Cessation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2618419A1 (de) * | 1975-04-28 | 1976-11-04 | Miles Lab | Heterogenes spezifisches bindungsverfahren zur bestimmung eines liganden in einem fluessigen medium und mittel zu dessen durchfuehrung |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2095830B (en) | 1983-03-23 |
| US4478817A (en) | 1984-10-23 |
| FR2409516A1 (fr) | 1979-06-15 |
| DE2849708C2 (en, 2012) | 1990-04-26 |
| CH645725A5 (fr) | 1984-10-15 |
| FR2409516B1 (fr) | 1985-05-17 |
| DE2849708A1 (de) | 1979-05-23 |
| SE7811630L (sv) | 1979-05-18 |
| GB2095830A (en) | 1982-10-06 |
| CA1113392A (en) | 1981-12-01 |
| CH640061A5 (fr) | 1983-12-15 |
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