DE2719704A1 - Verfahren zur bestimmung der lipase-aktivitaet, fuer die bestimmung zu verwendendes reagenz und verfahren zu seiner herstellung - Google Patents
Verfahren zur bestimmung der lipase-aktivitaet, fuer die bestimmung zu verwendendes reagenz und verfahren zu seiner herstellungInfo
- Publication number
- DE2719704A1 DE2719704A1 DE19772719704 DE2719704A DE2719704A1 DE 2719704 A1 DE2719704 A1 DE 2719704A1 DE 19772719704 DE19772719704 DE 19772719704 DE 2719704 A DE2719704 A DE 2719704A DE 2719704 A1 DE2719704 A1 DE 2719704A1
- Authority
- DE
- Germany
- Prior art keywords
- triglyceride
- reaction solution
- reagent
- impregnated
- aqueous reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims description 25
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 18
- 235000019626 lipase activity Nutrition 0.000 title claims description 15
- 238000004519 manufacturing process Methods 0.000 title description 4
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 64
- 239000002904 solvent Substances 0.000 claims description 23
- 239000007787 solid Substances 0.000 claims description 22
- 239000000839 emulsion Substances 0.000 claims description 20
- 239000011159 matrix material Substances 0.000 claims description 19
- PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 claims description 19
- BAECOWNUKCLBPZ-HIUWNOOHSA-N Triolein Natural products O([C@H](OCC(=O)CCCCCCC/C=C\CCCCCCCC)COC(=O)CCCCCCC/C=C\CCCCCCCC)C(=O)CCCCCCC/C=C\CCCCCCCC BAECOWNUKCLBPZ-HIUWNOOHSA-N 0.000 claims description 18
- PHYFQTYBJUILEZ-UHFFFAOYSA-N Trioleoylglycerol Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCCCCCCCC)COC(=O)CCCCCCCC=CCCCCCCCC PHYFQTYBJUILEZ-UHFFFAOYSA-N 0.000 claims description 18
- 229940117972 triolein Drugs 0.000 claims description 18
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 229920001223 polyethylene glycol Polymers 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 239000006193 liquid solution Substances 0.000 claims description 8
- 239000002202 Polyethylene glycol Substances 0.000 claims description 7
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 6
- 229930195725 Mannitol Natural products 0.000 claims description 6
- 239000000594 mannitol Substances 0.000 claims description 6
- 235000010355 mannitol Nutrition 0.000 claims description 6
- 229920006395 saturated elastomer Polymers 0.000 claims description 6
- 150000003626 triacylglycerols Chemical class 0.000 claims description 6
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 4
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 4
- 239000008121 dextrose Substances 0.000 claims description 4
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 4
- 229960000367 inositol Drugs 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 4
- 239000000600 sorbitol Substances 0.000 claims description 4
- 150000005846 sugar alcohols Chemical class 0.000 claims description 4
- 239000002671 adjuvant Substances 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims 1
- 239000000243 solution Substances 0.000 description 19
- 102000004882 Lipase Human genes 0.000 description 10
- 108090001060 Lipase Proteins 0.000 description 10
- 239000004367 Lipase Substances 0.000 description 9
- 235000019421 lipase Nutrition 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000005352 clarification Methods 0.000 description 2
- 235000021588 free fatty acids Nutrition 0.000 description 2
- 125000005456 glyceride group Chemical group 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000212384 Bifora Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 229940093761 bile salts Drugs 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229940009976 deoxycholate Drugs 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 208000024691 pancreas disease Diseases 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000008247 solid mixture Substances 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 125000005457 triglyceride group Chemical group 0.000 description 1
- 239000002569 water oil cream Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68297776A | 1976-05-04 | 1976-05-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2719704A1 true DE2719704A1 (de) | 1977-11-24 |
Family
ID=24742046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19772719704 Withdrawn DE2719704A1 (de) | 1976-05-04 | 1977-05-03 | Verfahren zur bestimmung der lipase-aktivitaet, fuer die bestimmung zu verwendendes reagenz und verfahren zu seiner herstellung |
Country Status (12)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0014252A1 (de) * | 1979-02-05 | 1980-08-20 | Roche Diagnostics GmbH | Reagens zur Lipasebestimmung und Verfahren zu seiner Herstellung |
| US5009994A (en) * | 1986-03-24 | 1991-04-23 | Em Diagnostic Systems, Inc. | Particles containing mannitol suitable for tabletting into diagnostic reagents |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5510447A (en) * | 1978-07-07 | 1980-01-24 | Nippon Electric Co | Oxide permittivity material |
| DE2905531A1 (de) * | 1979-02-14 | 1981-01-08 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten |
| EP0021572A1 (en) * | 1979-06-04 | 1981-01-07 | American Hospital Supply Corporation | A substrate for use in a turbidimetric assay for lipase and a method for making this substrate |
| US4555483A (en) * | 1982-08-11 | 1985-11-26 | Eastman Kodak Company | Methods, compositions and elements for the determination of lipase |
| US4820627A (en) * | 1986-03-24 | 1989-04-11 | Em Diagnostic Systems, Inc. | Method of preparing particles suitable for tabletting into diagnostic reagents |
| FR2619219A1 (fr) * | 1987-08-07 | 1989-02-10 | Strasbourg I Louis Pasteur Uni | Reactif sec pour la determination de la lipase pancreatique, procede de preparation dudit reactif sec et reactif sec et reactif sous forme d'emulsion obtenu par reconstitution du reactif sec. |
| JP2711332B2 (ja) * | 1988-04-15 | 1998-02-10 | 株式会社ヤトロン | 非水溶性物質の透明な水溶液が得られる凍結乾燥品の製造方法 |
| FR2824334B1 (fr) * | 2001-05-03 | 2003-10-10 | Coletica | Procede pour tester une substance eventuellement active dans le domaine de la lipolyse et son utilisation principalement cosmetique |
| US20090093007A1 (en) * | 2007-05-16 | 2009-04-09 | Shigeki Kageyama | Method for producing dry analytical element for pancreatic lipase measurement |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1961983B2 (de) * | 1969-12-10 | 1972-08-31 | Substrat fuer die bestimmung der lipase-aktivitaet und verfahren zur herstellung des substrats | |
| DE2225608B2 (de) * | 1972-05-26 | 1975-07-10 | C.H. Boehringer Sohn, 6507 Ingelheim | Verfahren und Mittel zur Bestimmung der Lipase in Körperflüssigkeiten |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3539450A (en) * | 1966-06-30 | 1970-11-10 | Calbiochem | Stabilization of enzymes |
| US3917515A (en) * | 1974-03-13 | 1975-11-04 | Jack M Goldberg | Serum lipase method and medium |
-
1977
- 1977-04-15 GB GB1585977A patent/GB1530238A/en not_active Expired
- 1977-05-02 CA CA277,628A patent/CA1109373A/en not_active Expired
- 1977-05-02 NL NL7704782A patent/NL7704782A/xx not_active Application Discontinuation
- 1977-05-03 DE DE19772719704 patent/DE2719704A1/de not_active Withdrawn
- 1977-05-03 DK DK194577A patent/DK194577A/da unknown
- 1977-05-03 IT IT2313477A patent/IT1114613B/it active
- 1977-05-03 FR FR7713341A patent/FR2350604A1/fr active Granted
- 1977-05-03 CH CH554077A patent/CH628929A5/de not_active IP Right Cessation
- 1977-05-03 BE BE177233A patent/BE854219A/xx not_active IP Right Cessation
- 1977-05-04 JP JP5194277A patent/JPS52134499A/ja active Pending
- 1977-05-04 IE IE90777A patent/IE44834B1/en unknown
- 1977-05-04 LU LU77256A patent/LU77256A1/xx unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1961983B2 (de) * | 1969-12-10 | 1972-08-31 | Substrat fuer die bestimmung der lipase-aktivitaet und verfahren zur herstellung des substrats | |
| DE2225608B2 (de) * | 1972-05-26 | 1975-07-10 | C.H. Boehringer Sohn, 6507 Ingelheim | Verfahren und Mittel zur Bestimmung der Lipase in Körperflüssigkeiten |
Non-Patent Citations (1)
| Title |
|---|
| Bergmeyer, H.U.: Methoden der Enzymatischen Analyse, 3. Aufl., Bd. I, S. 843-848, 1974 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0014252A1 (de) * | 1979-02-05 | 1980-08-20 | Roche Diagnostics GmbH | Reagens zur Lipasebestimmung und Verfahren zu seiner Herstellung |
| US5009994A (en) * | 1986-03-24 | 1991-04-23 | Em Diagnostic Systems, Inc. | Particles containing mannitol suitable for tabletting into diagnostic reagents |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS52134499A (en) | 1977-11-10 |
| FR2350604B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1983-08-05 |
| DK194577A (da) | 1977-11-05 |
| GB1530238A (en) | 1978-10-25 |
| BE854219A (fr) | 1977-11-03 |
| IE44834L (en) | 1977-11-04 |
| IT1114613B (it) | 1986-01-27 |
| CH628929A5 (en) | 1982-03-31 |
| IE44834B1 (en) | 1982-04-07 |
| LU77256A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1977-12-13 |
| FR2350604A1 (fr) | 1977-12-02 |
| CA1109373A (en) | 1981-09-22 |
| NL7704782A (nl) | 1977-11-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8125 | Change of the main classification |
Ipc: C12Q 1/34 |
|
| 8130 | Withdrawal |