IE44834L - Determining lipase activity - Google Patents

Determining lipase activity

Info

Publication number
IE44834L
IE44834L IE90777A IE90777A IE44834L IE 44834 L IE44834 L IE 44834L IE 90777 A IE90777 A IE 90777A IE 90777 A IE90777 A IE 90777A IE 44834 L IE44834 L IE 44834L
Authority
IE
Ireland
Prior art keywords
triglyceride
carrier
lipase activity
soluble
impregnated
Prior art date
Application number
IE90777A
Other versions
IE44834B1 (en
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of IE44834L publication Critical patent/IE44834L/en
Publication of IE44834B1 publication Critical patent/IE44834B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The reagent for the determination of lipase activity in a solution consists of a solid matrix of an inert carrier which is soluble in an aqueous reaction solution and is impregnated with a triglyceride. The carrier is preferably impregnated with triolein; the carrier is, for example, a sugar. To prepare the reagent, at least one triglyceride is dissolved in a solvent. The resulting solution is then mixed with an appropriate amount of the inert carrier which is soluble in an aqueous reaction solution. Finally, the solvent is evaporated off so that a solid matrix of the carrier impregnated with the triglyceride remains. The lipase activity in a sample is determined by reacting the sample with an emulsion of a triglyceride in an aqueous reaction solution and measuring the rate at which the triglyceride is hydrolysed to soluble fragments. [GB1530238A]
IE90777A 1976-05-04 1977-05-04 Method of determining lipase activity using a novel triglyceride reagent and method for preparing that reagent IE44834B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US68297776A 1976-05-04 1976-05-04

Publications (2)

Publication Number Publication Date
IE44834L true IE44834L (en) 1977-11-04
IE44834B1 IE44834B1 (en) 1982-04-07

Family

ID=24742046

Family Applications (1)

Application Number Title Priority Date Filing Date
IE90777A IE44834B1 (en) 1976-05-04 1977-05-04 Method of determining lipase activity using a novel triglyceride reagent and method for preparing that reagent

Country Status (12)

Country Link
JP (1) JPS52134499A (en)
BE (1) BE854219A (en)
CA (1) CA1109373A (en)
CH (1) CH628929A5 (en)
DE (1) DE2719704A1 (en)
DK (1) DK194577A (en)
FR (1) FR2350604A1 (en)
GB (1) GB1530238A (en)
IE (1) IE44834B1 (en)
IT (1) IT1114613B (en)
LU (1) LU77256A1 (en)
NL (1) NL7704782A (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5510447A (en) * 1978-07-07 1980-01-24 Nippon Electric Co Oxide permittivity material
DE2904305C2 (en) * 1979-02-05 1981-07-02 Boehringer Mannheim Gmbh, 6800 Mannheim Lipase determination reagent and process for its preparation
DE2905531A1 (en) * 1979-02-14 1981-01-08 Boehringer Mannheim Gmbh DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS
EP0021572A1 (en) * 1979-06-04 1981-01-07 American Hospital Supply Corporation A substrate for use in a turbidimetric assay for lipase and a method for making this substrate
US4555483A (en) * 1982-08-11 1985-11-26 Eastman Kodak Company Methods, compositions and elements for the determination of lipase
US5009994A (en) * 1986-03-24 1991-04-23 Em Diagnostic Systems, Inc. Particles containing mannitol suitable for tabletting into diagnostic reagents
US4820627A (en) * 1986-03-24 1989-04-11 Em Diagnostic Systems, Inc. Method of preparing particles suitable for tabletting into diagnostic reagents
FR2619219A1 (en) * 1987-08-07 1989-02-10 Strasbourg I Louis Pasteur Uni Dry reagent for the determination of pancreatic lipase, process for preparing the said dry reagent and dry reagent and reagent in emulsion form obtained by reconstituting the dry reagent
JP2711332B2 (en) * 1988-04-15 1998-02-10 株式会社ヤトロン Method for producing freeze-dried product from which transparent aqueous solution of water-insoluble substance is obtained
FR2824334B1 (en) 2001-05-03 2003-10-10 Coletica METHOD FOR TESTING A SUBSTANCE POSSIBLY ACTIVE IN THE FIELD OF LIPOLYSIS AND ITS MAINLY COSMETIC USE
JP5463012B2 (en) * 2007-05-16 2014-04-09 富士フイルム株式会社 Method for producing dry analytical element for pancreatic lipase measurement

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539450A (en) * 1966-06-30 1970-11-10 Calbiochem Stabilization of enzymes
ZA707714B (en) * 1969-12-10 1971-09-29 Boehringer Mannheim Gmbh Reagent for the determination of lipase activity
US3917515A (en) * 1974-03-13 1975-11-04 Jack M Goldberg Serum lipase method and medium

Also Published As

Publication number Publication date
CH628929A5 (en) 1982-03-31
LU77256A1 (en) 1977-12-13
NL7704782A (en) 1977-11-08
DE2719704A1 (en) 1977-11-24
GB1530238A (en) 1978-10-25
CA1109373A (en) 1981-09-22
IT1114613B (en) 1986-01-27
DK194577A (en) 1977-11-05
JPS52134499A (en) 1977-11-10
FR2350604A1 (en) 1977-12-02
IE44834B1 (en) 1982-04-07
FR2350604B1 (en) 1983-08-05
BE854219A (en) 1977-11-03

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