GB1530238A - Method of determining lipase activity using a triglyceride reagent and method for preparing that reagent - Google Patents

Method of determining lipase activity using a triglyceride reagent and method for preparing that reagent

Info

Publication number
GB1530238A
GB1530238A GB1585977A GB1585977A GB1530238A GB 1530238 A GB1530238 A GB 1530238A GB 1585977 A GB1585977 A GB 1585977A GB 1585977 A GB1585977 A GB 1585977A GB 1530238 A GB1530238 A GB 1530238A
Authority
GB
United Kingdom
Prior art keywords
triglyceride
reagent
lipase activity
carrier
preparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1585977A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of GB1530238A publication Critical patent/GB1530238A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The reagent for the determination of lipase activity in a solution consists of a solid matrix of an inert carrier which is soluble in an aqueous reaction solution and is impregnated with a triglyceride. The carrier is preferably impregnated with triolein; the carrier is, for example, a sugar. To prepare the reagent, at least one triglyceride is dissolved in a solvent. The resulting solution is then mixed with an appropriate amount of the inert carrier which is soluble in an aqueous reaction solution. Finally, the solvent is evaporated off so that a solid matrix of the carrier impregnated with the triglyceride remains. The lipase activity in a sample is determined by reacting the sample with an emulsion of a triglyceride in an aqueous reaction solution and measuring the rate at which the triglyceride is hydrolysed to soluble fragments.
GB1585977A 1976-05-04 1977-04-15 Method of determining lipase activity using a triglyceride reagent and method for preparing that reagent Expired GB1530238A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US68297776A 1976-05-04 1976-05-04

Publications (1)

Publication Number Publication Date
GB1530238A true GB1530238A (en) 1978-10-25

Family

ID=24742046

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1585977A Expired GB1530238A (en) 1976-05-04 1977-04-15 Method of determining lipase activity using a triglyceride reagent and method for preparing that reagent

Country Status (12)

Country Link
JP (1) JPS52134499A (en)
BE (1) BE854219A (en)
CA (1) CA1109373A (en)
CH (1) CH628929A5 (en)
DE (1) DE2719704A1 (en)
DK (1) DK194577A (en)
FR (1) FR2350604A1 (en)
GB (1) GB1530238A (en)
IE (1) IE44834B1 (en)
IT (1) IT1114613B (en)
LU (1) LU77256A1 (en)
NL (1) NL7704782A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4555483A (en) * 1982-08-11 1985-11-26 Eastman Kodak Company Methods, compositions and elements for the determination of lipase
EP1992700A1 (en) * 2007-05-16 2008-11-19 FUJIFILM Corporation Method for producing dry analytical element for pancreatic lipase measurement

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5510447A (en) * 1978-07-07 1980-01-24 Nippon Electric Co Oxide permittivity material
DE2904305C2 (en) * 1979-02-05 1981-07-02 Boehringer Mannheim Gmbh, 6800 Mannheim Lipase determination reagent and process for its preparation
DE2905531A1 (en) * 1979-02-14 1981-01-08 Boehringer Mannheim Gmbh DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS
EP0021572A1 (en) * 1979-06-04 1981-01-07 American Hospital Supply Corporation A substrate for use in a turbidimetric assay for lipase and a method for making this substrate
US5009994A (en) * 1986-03-24 1991-04-23 Em Diagnostic Systems, Inc. Particles containing mannitol suitable for tabletting into diagnostic reagents
US4820627A (en) * 1986-03-24 1989-04-11 Em Diagnostic Systems, Inc. Method of preparing particles suitable for tabletting into diagnostic reagents
FR2619219A1 (en) * 1987-08-07 1989-02-10 Strasbourg I Louis Pasteur Uni Dry reagent for the determination of pancreatic lipase, process for preparing the said dry reagent and dry reagent and reagent in emulsion form obtained by reconstituting the dry reagent
JP2711332B2 (en) * 1988-04-15 1998-02-10 株式会社ヤトロン Method for producing freeze-dried product from which transparent aqueous solution of water-insoluble substance is obtained
FR2824334B1 (en) * 2001-05-03 2003-10-10 Coletica METHOD FOR TESTING A SUBSTANCE POSSIBLY ACTIVE IN THE FIELD OF LIPOLYSIS AND ITS MAINLY COSMETIC USE

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539450A (en) * 1966-06-30 1970-11-10 Calbiochem Stabilization of enzymes
ZA707714B (en) * 1969-12-10 1971-09-29 Boehringer Mannheim Gmbh Reagent for the determination of lipase activity
US3917515A (en) * 1974-03-13 1975-11-04 Jack M Goldberg Serum lipase method and medium

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4555483A (en) * 1982-08-11 1985-11-26 Eastman Kodak Company Methods, compositions and elements for the determination of lipase
EP1992700A1 (en) * 2007-05-16 2008-11-19 FUJIFILM Corporation Method for producing dry analytical element for pancreatic lipase measurement

Also Published As

Publication number Publication date
FR2350604B1 (en) 1983-08-05
DE2719704A1 (en) 1977-11-24
CA1109373A (en) 1981-09-22
NL7704782A (en) 1977-11-08
IE44834L (en) 1977-11-04
LU77256A1 (en) 1977-12-13
BE854219A (en) 1977-11-03
JPS52134499A (en) 1977-11-10
DK194577A (en) 1977-11-05
IT1114613B (en) 1986-01-27
IE44834B1 (en) 1982-04-07
CH628929A5 (en) 1982-03-31
FR2350604A1 (en) 1977-12-02

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee