ES475984A1 - Enzyme modifier immunoassay - Google Patents

Enzyme modifier immunoassay

Info

Publication number
ES475984A1
ES475984A1 ES475984A ES475984A ES475984A1 ES 475984 A1 ES475984 A1 ES 475984A1 ES 475984 A ES475984 A ES 475984A ES 475984 A ES475984 A ES 475984A ES 475984 A1 ES475984 A1 ES 475984A1
Authority
ES
Spain
Prior art keywords
enzyme
nature
degree
active material
immunoassay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES475984A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of ES475984A1 publication Critical patent/ES475984A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D475/00—Heterocyclic compounds containing pteridine ring systems
    • C07D475/06—Heterocyclic compounds containing pteridine ring systems with a nitrogen atom directly attached in position 4
    • C07D475/08—Heterocyclic compounds containing pteridine ring systems with a nitrogen atom directly attached in position 4 with a nitrogen atom directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D489/00—Heterocyclic compounds containing 4aH-8, 9 c- Iminoethano-phenanthro [4, 5-b, c, d] furan ring systems, e.g. derivatives of [4, 5-epoxy]-morphinan of the formula:
    • C07D489/02—Heterocyclic compounds containing 4aH-8, 9 c- Iminoethano-phenanthro [4, 5-b, c, d] furan ring systems, e.g. derivatives of [4, 5-epoxy]-morphinan of the formula: with oxygen atoms attached in positions 3 and 6, e.g. morphine, morphinone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J43/00—Normal steroids having a nitrogen-containing hetero ring spiro-condensed or not condensed with the cyclopenta(a)hydrophenanthrene skeleton
    • C07J43/003—Normal steroids having a nitrogen-containing hetero ring spiro-condensed or not condensed with the cyclopenta(a)hydrophenanthrene skeleton not condensed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a process for the determination of an immunologically active material in a sample. The process is characterised in that the sample is brought together with a receptor which is able to bind the immunologically active material specifically, with the immunologically active material, which is labelled with a substance which is able to modify the degree and the nature of the activity of the enzyme, and with an enzyme and an enzyme substrate, whereupon the degree or the nature of the enzyme activity obtained is measured and compared with that obtained using a standard. The invention further relates to a reagent for carrying out the process. A suitable substance which is able to modify the degree or the nature of the activity of an enzyme is in particular methotrexate.
ES475984A 1977-03-15 1978-12-01 Enzyme modifier immunoassay Expired ES475984A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB10859/77A GB1595101A (en) 1977-03-15 1977-03-15 Enzyme modifier immunoassay

Publications (1)

Publication Number Publication Date
ES475984A1 true ES475984A1 (en) 1979-06-16

Family

ID=9975639

Family Applications (2)

Application Number Title Priority Date Filing Date
ES467831A Expired ES467831A1 (en) 1977-03-15 1978-03-14 Enzyme modifier immunoassay
ES475984A Expired ES475984A1 (en) 1977-03-15 1978-12-01 Enzyme modifier immunoassay

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES467831A Expired ES467831A1 (en) 1977-03-15 1978-03-14 Enzyme modifier immunoassay

Country Status (16)

Country Link
JP (1) JPS53115814A (en)
AT (1) AT367203B (en)
AU (1) AU519326B2 (en)
BE (1) BE864856A (en)
CA (1) CA1102789A (en)
CH (1) CH641570A5 (en)
DE (1) DE2811257A1 (en)
DK (1) DK152313C (en)
ES (2) ES467831A1 (en)
FR (1) FR2384262A1 (en)
GB (1) GB1595101A (en)
IL (1) IL54234A0 (en)
IT (1) IT1158665B (en)
NL (1) NL7802845A (en)
NO (2) NO152955C (en)
SE (1) SE447026B (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4273866A (en) 1979-02-05 1981-06-16 Abbott Laboratories Ligand analog-irreversible enzyme inhibitor conjugates and methods for use
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
DE3006709A1 (en) * 1980-02-22 1981-08-27 Hans A. Dipl.-Chem. Dr. 8000 München Thoma HOMOGENEOUS METHOD FOR COMPETITIVE DETERMINATION OF LIGANDS
US4341865A (en) 1980-07-21 1982-07-27 Abbott Laboratories Determination of thyroxine binding globulin
FR2502786B1 (en) * 1981-03-24 1985-06-21 Stallergenes Laboratoire METHOD FOR FIXING ANTIGENS AND ANTIBODIES TO POLYSACCHARIDE SUPPORT, AND USE OF THE PRODUCT OBTAINED THEREFOR FOR IMMUNOASSAYS
GB2116979B (en) * 1982-02-25 1985-05-15 Ward Page Faulk Conjugates of proteins with anti-tumour agents
AU574646B2 (en) * 1982-07-19 1988-07-14 Cooperbiomedical Inc. Enzyme assay method
GB2135773B (en) * 1983-01-31 1985-12-04 Boots Celltech Diagnostics Enzyme inhibitor labelled immunoassay
US4650751A (en) * 1983-04-29 1987-03-17 Technicon Instruments Corporation Protected binding assay avoiding non-specific protein interference
JPS607362A (en) * 1983-06-27 1985-01-16 Fujirebio Inc Measurement of antigen determinant-containing substance using enzyme
US4837395A (en) * 1985-05-10 1989-06-06 Syntex (U.S.A.) Inc. Single step heterogeneous assay
CA1330378C (en) * 1986-05-08 1994-06-21 Daniel J. Coughlin Amine derivatives of folic acid analogs
US4939264A (en) * 1986-07-14 1990-07-03 Abbott Laboratories Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies
IL85596A (en) * 1987-05-18 1992-06-21 Technicon Instr Method for a specific binding enzyme immunoassay
US5972630A (en) * 1991-08-19 1999-10-26 Dade Behring Marburg Gmbh Homogeneous immunoassays using enzyme inhibitors
US5965106A (en) * 1992-03-04 1999-10-12 Perimmune Holdings, Inc. In vivo binding pair pretargeting
EP0590109A4 (en) * 1992-03-04 1995-04-19 Akzo Nv -i(IN VIVO) BINDING PAIR PRETARGETING.
EP1079231A4 (en) * 1998-05-15 2003-07-23 Sekisui Chemical Co Ltd Immunoassay reagents and immunoassay method
US6811998B2 (en) 1999-06-25 2004-11-02 Roche Diagnostics Operations, Inc. Conjugates of uncompetitive inhibitors of inosine monophosphate dehydrogenase
US8394813B2 (en) * 2000-11-14 2013-03-12 Shire Llc Active agent delivery systems and methods for protecting and administering active agents
US8664181B2 (en) * 2007-02-16 2014-03-04 Ktb Tumorforschungsgesellschaft Mbh Dual acting prodrugs

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2308005A1 (en) * 1975-04-15 1976-11-12 Hadaway Robert Drive in wall plug fastener - has body provided with indentations and covered with pliable externally smooth coating

Also Published As

Publication number Publication date
NO154814B (en) 1986-09-15
ES467831A1 (en) 1979-09-01
GB1595101A (en) 1981-08-05
FR2384262A1 (en) 1978-10-13
CA1102789A (en) 1981-06-09
DK152313B (en) 1988-02-15
AT367203B (en) 1982-06-11
NO152955B (en) 1985-09-09
SE447026B (en) 1986-10-20
NL7802845A (en) 1978-09-19
NO844764L (en) 1978-09-18
NO152955C (en) 1985-12-18
NO154814C (en) 1986-12-29
ATA182178A (en) 1981-10-15
DE2811257A1 (en) 1978-09-21
NO780902L (en) 1978-09-18
DK114778A (en) 1978-09-16
DK152313C (en) 1988-09-26
IL54234A0 (en) 1978-06-15
JPS53115814A (en) 1978-10-09
BE864856A (en) 1978-09-14
FR2384262B1 (en) 1983-04-08
IT1158665B (en) 1987-02-25
AU519326B2 (en) 1981-11-26
AU3396578A (en) 1979-09-13
CH641570A5 (en) 1984-02-29
SE7802923L (en) 1978-09-16
IT7821256A0 (en) 1978-03-15

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19991102