DE2217911A1 - Verfahren zur Herstellung von Desoxyribonucleinsäuren mit erhöhtem Molekulargewicht - Google Patents
Verfahren zur Herstellung von Desoxyribonucleinsäuren mit erhöhtem MolekulargewichtInfo
- Publication number
- DE2217911A1 DE2217911A1 DE19722217911 DE2217911A DE2217911A1 DE 2217911 A1 DE2217911 A1 DE 2217911A1 DE 19722217911 DE19722217911 DE 19722217911 DE 2217911 A DE2217911 A DE 2217911A DE 2217911 A1 DE2217911 A1 DE 2217911A1
- Authority
- DE
- Germany
- Prior art keywords
- phenol
- solution
- bentonite
- treatment
- aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 17
- 108020004414 DNA Proteins 0.000 title claims description 14
- 102000053602 DNA Human genes 0.000 title claims description 5
- 238000002360 preparation method Methods 0.000 title description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 23
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims description 8
- 239000000440 bentonite Substances 0.000 claims description 7
- 229910000278 bentonite Inorganic materials 0.000 claims description 7
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 7
- 108010061100 Nucleoproteins Proteins 0.000 claims description 5
- 102000011931 Nucleoproteins Human genes 0.000 claims description 5
- 229920006395 saturated elastomer Polymers 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 4
- 239000008267 milk Substances 0.000 claims description 4
- 235000013336 milk Nutrition 0.000 claims description 4
- 210000004080 milk Anatomy 0.000 claims description 4
- 239000011775 sodium fluoride Substances 0.000 claims description 4
- 235000013024 sodium fluoride Nutrition 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000005909 Kieselgur Substances 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims 5
- 150000001447 alkali salts Chemical class 0.000 claims 3
- 239000007864 aqueous solution Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 241000252203 Clupea harengus Species 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 229940122426 Nuclease inhibitor Drugs 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 102000007327 Protamines Human genes 0.000 description 1
- 108010007568 Protamines Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000003544 deproteinization Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011085 pressure filtration Methods 0.000 description 1
- 229940070353 protamines Drugs 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH559871A CH529769A (fr) | 1971-04-16 | 1971-04-16 | Procédé de préparation d'acides désoxyribonucléiques à poids moléculaire élevé |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2217911A1 true DE2217911A1 (de) | 1972-11-09 |
Family
ID=4296285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19722217911 Pending DE2217911A1 (de) | 1971-04-16 | 1972-04-13 | Verfahren zur Herstellung von Desoxyribonucleinsäuren mit erhöhtem Molekulargewicht |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US3838148A (enExample) |
| JP (1) | JPS4848500A (enExample) |
| BE (1) | BE781609A (enExample) |
| CA (2) | CA961034A (enExample) |
| CH (1) | CH529769A (enExample) |
| DE (1) | DE2217911A1 (enExample) |
| DK (1) | DK131294B (enExample) |
| ES (1) | ES401831A1 (enExample) |
| FR (1) | FR2133634B1 (enExample) |
| GB (1) | GB1392330A (enExample) |
| IE (1) | IE36280B1 (enExample) |
| IT (1) | IT986015B (enExample) |
| LU (1) | LU65120A1 (enExample) |
| NL (1) | NL7204889A (enExample) |
| SE (1) | SE386187B (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750671A (en) * | 1996-11-13 | 1998-05-12 | Khon; Trinh Cam | Method for isolating DNA from biological cells |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4654647A (en) * | 1984-09-24 | 1987-03-31 | Wedam Jack M | Finger actuated electronic control apparatus |
| US5182269A (en) * | 1987-09-23 | 1993-01-26 | Crinos Industria Farmacobiologica Spa | Composition for topical use having hair stimulating, anti-dandruff and anti-seborrhoic activity |
| FR2628440B1 (fr) * | 1988-03-14 | 1991-01-04 | Davigel Sa | Procede de preparation industrielle d'acide desoxyribonucleique a poids moleculaire eleve et a grande purete |
| JP2548494B2 (ja) * | 1992-07-27 | 1996-10-30 | 三光純薬株式会社 | 核酸抽出方法 |
| EP1206531A1 (en) * | 1999-08-26 | 2002-05-22 | Terje Strom | A method for preparation of dna from biological material |
| KR100320753B1 (ko) | 1999-10-23 | 2002-01-17 | 박한오 | 어류의 정원세포로부터 ｄｎａ 추출방법 |
| US6661140B2 (en) * | 2001-12-11 | 2003-12-09 | Black & Decker Inc. | Brushless motor having housing enabling alignment of stator and sensor |
| DE60318570T2 (de) | 2002-04-26 | 2009-01-08 | Kaneka Corp. | Härtbare zusammensetzung, härtendes produkt, herstellungsverfahren dafür und mit dem gehärteten produkt versiegelte lichtemittierende diode |
| EP2343326B1 (en) | 2008-10-02 | 2018-08-15 | Kaneka Corporation | Photocurable composition and cured product |
-
1971
- 1971-04-16 CH CH559871A patent/CH529769A/fr not_active IP Right Cessation
-
1972
- 1972-03-31 BE BE781609A patent/BE781609A/xx unknown
- 1972-04-06 LU LU65120D patent/LU65120A1/xx unknown
- 1972-04-07 IT IT22824/72A patent/IT986015B/it active
- 1972-04-10 GB GB1648172A patent/GB1392330A/en not_active Expired
- 1972-04-11 US US00243070A patent/US3838148A/en not_active Expired - Lifetime
- 1972-04-11 IE IE475/72A patent/IE36280B1/xx unknown
- 1972-04-12 FR FR7212732A patent/FR2133634B1/fr not_active Expired
- 1972-04-12 NL NL7204889A patent/NL7204889A/xx unknown
- 1972-04-13 DK DK178772AA patent/DK131294B/da unknown
- 1972-04-13 DE DE19722217911 patent/DE2217911A1/de active Pending
- 1972-04-14 CA CA139,773A patent/CA961034A/en not_active Expired
- 1972-04-14 SE SE7204882A patent/SE386187B/xx unknown
- 1972-04-17 JP JP47038565A patent/JPS4848500A/ja active Pending
- 1972-04-17 ES ES401831A patent/ES401831A1/es not_active Expired
- 1972-07-19 CA CA147,525A patent/CA981475A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750671A (en) * | 1996-11-13 | 1998-05-12 | Khon; Trinh Cam | Method for isolating DNA from biological cells |
Also Published As
| Publication number | Publication date |
|---|---|
| IE36280B1 (en) | 1976-09-29 |
| IE36280L (en) | 1972-10-16 |
| CH529769A (fr) | 1972-10-31 |
| DK131294B (da) | 1975-06-23 |
| FR2133634B1 (enExample) | 1977-04-01 |
| JPS4848500A (enExample) | 1973-07-09 |
| FR2133634A1 (enExample) | 1972-12-01 |
| IT986015B (it) | 1975-01-10 |
| US3838148A (en) | 1974-09-24 |
| ES401831A1 (es) | 1975-03-01 |
| LU65120A1 (enExample) | 1972-07-12 |
| NL7204889A (enExample) | 1972-10-18 |
| GB1392330A (en) | 1975-04-30 |
| BE781609A (fr) | 1972-07-17 |
| DK131294C (enExample) | 1975-11-17 |
| CA981475A (en) | 1976-01-13 |
| SE386187B (sv) | 1976-08-02 |
| CA961034A (en) | 1975-01-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHA | Expiration of time for request for examination |