DD297187A5 - Wirksame herstellung von mutantenproteasen - Google Patents

Wirksame herstellung von mutantenproteasen Download PDF

Info

Publication number
DD297187A5
DD297187A5 DD90343328A DD34332890A DD297187A5 DD 297187 A5 DD297187 A5 DD 297187A5 DD 90343328 A DD90343328 A DD 90343328A DD 34332890 A DD34332890 A DD 34332890A DD 297187 A5 DD297187 A5 DD 297187A5
Authority
DD
German Democratic Republic
Prior art keywords
protease
gene
strain
plasmid
bacillus
Prior art date
Application number
DD90343328A
Other languages
German (de)
English (en)
Inventor
Johannes C Van Der Laan
Christiaan A G Van Eekelen
Original Assignee
����`��������@�K@�Kk��
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8202457&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DD297187(A5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ����`��������@�K@�Kk�� filed Critical ����`��������@�K@�Kk��
Publication of DD297187A5 publication Critical patent/DD297187A5/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Mushroom Cultivation (AREA)
DD90343328A 1989-08-11 1990-08-08 Wirksame herstellung von mutantenproteasen DD297187A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP89202117 1989-08-11

Publications (1)

Publication Number Publication Date
DD297187A5 true DD297187A5 (de) 1992-01-02

Family

ID=8202457

Family Applications (1)

Application Number Title Priority Date Filing Date
DD90343328A DD297187A5 (de) 1989-08-11 1990-08-08 Wirksame herstellung von mutantenproteasen

Country Status (19)

Country Link
US (1) US20040014175A1 (pt)
EP (1) EP0414297B1 (pt)
JP (1) JP3490435B2 (pt)
KR (1) KR0169978B1 (pt)
CN (1) CN1049866A (pt)
AT (1) ATE144283T1 (pt)
AU (1) AU629970B2 (pt)
BG (1) BG60567B1 (pt)
BR (1) BR9003956A (pt)
CA (1) CA2023094C (pt)
DD (1) DD297187A5 (pt)
DE (1) DE69028890T2 (pt)
DK (1) DK0414297T3 (pt)
ES (1) ES2095233T3 (pt)
FI (1) FI110365B (pt)
GR (1) GR3021669T3 (pt)
IE (1) IE77037B1 (pt)
PT (1) PT94961B (pt)
RU (1) RU2060276C1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
ATE301719T1 (de) 1994-06-17 2005-08-15 Genencor Int Von der alpha-amylase aus b. licheniformis abgeleitete amylolytische enzyme mit verbesserten eigenschaften
DK0894126T3 (da) * 1996-03-27 2006-06-12 Novozymes As Alkalisk phosphatase-deficient trådformet svamp
JP3440694B2 (ja) * 1996-05-16 2003-08-25 ライオン株式会社 口腔用組成物
GB0024554D0 (en) 2000-10-06 2000-11-22 Agrol Ltd Ethanol production
WO2003083125A1 (en) 2002-03-29 2003-10-09 Genencor International, Inc. Ehanced protein expression in bacillus
US7968091B2 (en) 2005-02-16 2011-06-28 The General Hospital Corporation Methods and compositions to regulate iron metabolism
RU2510821C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) Федеральный Университет" (ФГАОУ ВПО КФУ) Штамм бактерий bacillus pumilus мк-10 с низкой протеолитической активностью и его применение
RU2511416C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) федеральный университет" (ФГАО ВПО КФУ) Штамм бактерий bacillus pumilus 2a-5 с низкой протеолитической активностью, повышенной активностью фосфатазы, способ его получения и применения

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (pt) * 1967-10-03 1971-06-03
JPS6055118B2 (ja) * 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
JP2637532B2 (ja) * 1987-02-27 1997-08-06 ギスト ブロカデス ナームローゼ フェンノートチャップ 原核微生物の染色体dnaにおける安定した遺伝子増幅
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用

Also Published As

Publication number Publication date
US20040014175A1 (en) 2004-01-22
CA2023094C (en) 2000-03-28
GR3021669T3 (en) 1997-02-28
BG92653A (bg) 1993-12-24
JP3490435B2 (ja) 2004-01-26
DE69028890D1 (de) 1996-11-21
CA2023094A1 (en) 1991-02-12
ES2095233T3 (es) 1997-02-16
KR0169978B1 (ko) 1999-02-01
DK0414297T3 (da) 1997-07-07
FI110365B (fi) 2002-12-31
EP0414297B1 (en) 1996-10-16
AU629970B2 (en) 1992-10-15
PT94961A (pt) 1991-04-18
PT94961B (pt) 1997-05-28
IE902904A1 (en) 1991-02-27
FI903927A0 (fi) 1990-08-08
BR9003956A (pt) 1991-09-03
KR910004805A (ko) 1991-03-29
BG60567B1 (bg) 1995-08-28
IE77037B1 (en) 1997-11-19
DE69028890T2 (de) 1997-02-27
CN1049866A (zh) 1991-03-13
JPH03210177A (ja) 1991-09-13
EP0414297A1 (en) 1991-02-27
AU6096190A (en) 1991-02-14
RU2060276C1 (ru) 1996-05-20
ATE144283T1 (de) 1996-11-15

Similar Documents

Publication Publication Date Title
DE3486139T2 (de) Bacillus, der weder Subtilisin noch neutrale Protease ausscheiden kann.
DE3853328T2 (de) Subtilisin-analoge.
DE3688920T2 (de) Hybride Polypeptide und Verfahren zu deren Herstellung.
DE69033388T2 (de) Alkalisches proteolytisches enzym und verfahren zur herstellung
DE3856331T2 (de) Molekulare Klonierung und Expression von proteolytische Enzyme kodierenden Genen
DE3883041T2 (de) Stabile Genamplifizierung in prokaryotischer chromosonaler DNA.
DE69028040T2 (de) Mehrfach mutierte Subtilisine
DE68926163T2 (de) Mutierte subtilisingene
DE69833652T2 (de) System zum exprimieren einer hyperthermostabilen protease
Camilli et al. Insertional mutagenesis of Listeria monocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions
DE3750916T2 (de) Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
EP0415296B2 (de) Neue hochalkalische Proteasen
DE68926333T2 (de) Bacillus-Stämme
EP0995801A1 (de) Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme
DE69723855T2 (de) Verfahren zur herstellung von polypeptiden in surfaktinmutanten von bacillus zellen
DE69615055T2 (de) Gene kodierend für ultrathermostabile proteasen
DD297187A5 (de) Wirksame herstellung von mutantenproteasen
DE69837086T2 (de) Verbesserte prokariontische proteinenexpression
DE69026260T2 (de) Induzierbare Expressionsvektoren
DE69230825T2 (de) Genexpression in bazillen
EP0572992A1 (de) Alkalische Proteasen aus Bacillus pumilus
DE3586410T2 (de) Verfahren zur herstellung von neutraler protease.
DE69105188T2 (de) Asporogener Stamm von Bacillus substilis und seine Verwendung als Wirt in der Herstellung von heterologen Produkten.
EP0503346A2 (de) Verfahren zur Verbesserung der Stabilität von hochalkalischen Proteasen
EP0610808A2 (de) Verbesserte hochalkalische Proteasen

Legal Events

Date Code Title Description
ENJ Ceased due to non-payment of renewal fee