DE69028040T2 - Mehrfach mutierte Subtilisine - Google Patents

Mehrfach mutierte Subtilisine

Info

Publication number
DE69028040T2
DE69028040T2 DE69028040T DE69028040T DE69028040T2 DE 69028040 T2 DE69028040 T2 DE 69028040T2 DE 69028040 T DE69028040 T DE 69028040T DE 69028040 T DE69028040 T DE 69028040T DE 69028040 T2 DE69028040 T2 DE 69028040T2
Authority
DE
Germany
Prior art keywords
multiple mutant
subtilisins
mutant subtilisins
subtilisin
admixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69028040T
Other languages
English (en)
Other versions
DE69028040D1 (de
Inventor
Mark M Zukowski
Linda O Narhi
Michael Levitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of DE69028040D1 publication Critical patent/DE69028040D1/de
Publication of DE69028040T2 publication Critical patent/DE69028040T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)
DE69028040T 1989-05-17 1990-05-01 Mehrfach mutierte Subtilisine Expired - Lifetime DE69028040T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17

Publications (2)

Publication Number Publication Date
DE69028040D1 DE69028040D1 (de) 1996-09-19
DE69028040T2 true DE69028040T2 (de) 1997-02-27

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69028040T Expired - Lifetime DE69028040T2 (de) 1989-05-17 1990-05-01 Mehrfach mutierte Subtilisine

Country Status (14)

Country Link
US (1) US5397705A (de)
EP (1) EP0398539B1 (de)
JP (1) JPH03505976A (de)
KR (1) KR100188532B1 (de)
AT (1) ATE141324T1 (de)
AU (1) AU618675B2 (de)
CA (1) CA2016211C (de)
DE (1) DE69028040T2 (de)
DK (1) DK0398539T3 (de)
ES (1) ES2091224T3 (de)
GR (1) GR3021010T3 (de)
HK (1) HK1006857A1 (de)
SG (1) SG47797A1 (de)
WO (1) WO1990014420A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0916732B1 (de) * 1987-04-06 2007-08-22 Novozymes A/S Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
AU2591992A (en) * 1991-10-16 1993-05-21 Unilever Plc Aqueous enzymatic detergent compositions
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
BR9407833A (pt) * 1993-10-14 1997-05-13 Procter & Gamble Composições alvejantes compreendendo enzimas protease
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
DE69720043T2 (de) * 1996-12-20 2003-10-16 Unilever Nv Enzymatische bleichmittelzusammensetzung
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
WO1999049056A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
AU5928100A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
CZ2002212A3 (cs) 1999-07-22 2002-07-17 The Procter & Gamble Company Varianty subtilisinové proteasy mající aminokyselinové substituce v definovaných epitopových oblastech
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CA2379729A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Protease conjugates having sterically protected clip sites
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
EP2336331A1 (de) * 1999-08-31 2011-06-22 Novozymes A/S Neue Proteasen und Varianten davon
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
WO2002010183A1 (en) * 2000-07-31 2002-02-07 Menzel, Rolf Compositions and methods for directed gene assembly
EP1998266A3 (de) 2001-02-19 2009-02-11 Merck Patent GmbH Verfahren zur Identifizierung von T-Zell-Epitopen und Verfahren zur Herstellung von Molekülen mit verminderter Immunogenität
CA2441595C (en) * 2001-03-23 2012-07-03 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
US20050227229A1 (en) * 2001-07-09 2005-10-13 Lebo Roger V Multiple controls for molecular genetic analyses
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
EP2287321B1 (de) * 2002-01-16 2014-08-13 Danisco US Inc. Mehrfach substituierte Proteasevarianten
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
US7507569B2 (en) * 2003-05-07 2009-03-24 Novozymes A/S Variant subtilisin enzymes (subtilases)
EP3887515A1 (de) * 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin-varianten mit verbesserter stabilität
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
EP0254735B2 (de) * 1986-01-15 1998-06-17 Amgen Inc. Herstellung von ph- und wärmestabile subtilisinanaloge
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
ES2068181T3 (es) * 1986-04-30 1995-04-16 Genencor Int Mutantes de la carbonil hidrolasa no humana, secuencias de adn y vectores que codifican las mismas y huespedes transformados con dichos vectores.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
EP0916732B1 (de) * 1987-04-06 2007-08-22 Novozymes A/S Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
US5397705A (en) 1995-03-14
DK0398539T3 (da) 1996-12-09
EP0398539A1 (de) 1990-11-22
ES2091224T3 (es) 1996-11-01
EP0398539B1 (de) 1996-08-14
KR920701426A (ko) 1992-08-11
AU618675B2 (en) 1992-01-02
SG47797A1 (en) 1998-04-17
HK1006857A1 (en) 1999-03-19
CA2016211C (en) 1998-07-21
JPH03505976A (ja) 1991-12-26
DE69028040D1 (de) 1996-09-19
ATE141324T1 (de) 1996-08-15
CA2016211A1 (en) 1990-11-17
GR3021010T3 (en) 1996-12-31
KR100188532B1 (ko) 1999-06-01
WO1990014420A1 (en) 1990-11-29
AU5558090A (en) 1990-12-18

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Legal Events

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