ES2091224T3 - Subtilisinas con mutaciones multiples. - Google Patents

Subtilisinas con mutaciones multiples.

Info

Publication number
ES2091224T3
ES2091224T3 ES90304715T ES90304715T ES2091224T3 ES 2091224 T3 ES2091224 T3 ES 2091224T3 ES 90304715 T ES90304715 T ES 90304715T ES 90304715 T ES90304715 T ES 90304715T ES 2091224 T3 ES2091224 T3 ES 2091224T3
Authority
ES
Spain
Prior art keywords
subtilisins
multiple mutations
subtilisin
admixture
expressing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90304715T
Other languages
English (en)
Inventor
Mark M Zukowski
Linda O Narhi
Michael Levitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2091224T3 publication Critical patent/ES2091224T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)

Abstract

UNA CLASE DE ANALOGOS DE LA SUBTILISINA ADECUADA PARA SER MEZCLADA CON COMPOSICIONES DE LIMPIEZA Y CON UNA ESTABILIDAD MEJORADA SOBRE EL BACILO DE LA SUBTISILINA. ESTOS ANALOGOS SON PREPARADOS EXPRESANDO UN GEN MODIFICADO CODIFICADOR DEL ANALOGO DE LA SUBTILISINA EN EL BACILLUS SUBTILIS.
ES90304715T 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples. Expired - Lifetime ES2091224T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17

Publications (1)

Publication Number Publication Date
ES2091224T3 true ES2091224T3 (es) 1996-11-01

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90304715T Expired - Lifetime ES2091224T3 (es) 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples.

Country Status (14)

Country Link
US (1) US5397705A (es)
EP (1) EP0398539B1 (es)
JP (1) JPH03505976A (es)
KR (1) KR100188532B1 (es)
AT (1) ATE141324T1 (es)
AU (1) AU618675B2 (es)
CA (1) CA2016211C (es)
DE (1) DE69028040T2 (es)
DK (1) DK0398539T3 (es)
ES (1) ES2091224T3 (es)
GR (1) GR3021010T3 (es)
HK (1) HK1006857A1 (es)
SG (1) SG47797A1 (es)
WO (1) WO1990014420A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3856362T2 (de) * 1987-04-06 2000-05-18 Novo Nordisk A/S, Bagsvaerd Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
WO1993008253A1 (en) * 1991-10-16 1993-04-29 Unilever N.V. Aqueous enzymatic detergent compositions
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
CZ105296A3 (en) * 1993-10-14 1996-09-11 Procter & Gamble Bleaching agent containing protease enzymes, cleansing agents, agent for cleaning fabrics and fabric cleaning method
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
DE69720043T2 (de) * 1996-12-20 2003-10-16 Unilever N.V., Rotterdam Enzymatische bleichmittelzusammensetzung
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
WO1999049056A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
CZ2002212A3 (cs) 1999-07-22 2002-07-17 The Procter & Gamble Company Varianty subtilisinové proteasy mající aminokyselinové substituce v definovaných epitopových oblastech
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
NZ517409A (en) 1999-08-31 2004-05-28 Novozymes As RP-II properties with amino acid substitutions used in detergent compositions and additives
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
AU2001280968A1 (en) * 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
HUP0303199A2 (hu) 2001-02-19 2003-12-29 Merck Patent Gmbh Eljárás T-sejt-epitópok azonosítására és csökkentett immunogenitású molekulák előállítására
CA2441595C (en) * 2001-03-23 2012-07-03 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
CA2455607C (en) * 2001-07-09 2013-01-08 Children's Hospital Medical Center Of Akron Multiple controls for molecular genetic analyses
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
JP5328002B2 (ja) * 2002-01-16 2013-10-30 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
BRPI0409992A (pt) * 2003-05-07 2006-05-09 Novozymes As enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar
US20230028935A1 (en) * 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
DE3856362T2 (de) * 1987-04-06 2000-05-18 Novo Nordisk A/S, Bagsvaerd Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
KR100188532B1 (ko) 1999-06-01
CA2016211C (en) 1998-07-21
AU618675B2 (en) 1992-01-02
WO1990014420A1 (en) 1990-11-29
DK0398539T3 (da) 1996-12-09
EP0398539A1 (en) 1990-11-22
KR920701426A (ko) 1992-08-11
DE69028040D1 (de) 1996-09-19
SG47797A1 (en) 1998-04-17
US5397705A (en) 1995-03-14
ATE141324T1 (de) 1996-08-15
JPH03505976A (ja) 1991-12-26
AU5558090A (en) 1990-12-18
EP0398539B1 (en) 1996-08-14
CA2016211A1 (en) 1990-11-17
DE69028040T2 (de) 1997-02-27
HK1006857A1 (en) 1999-03-19
GR3021010T3 (en) 1996-12-31

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