ES2091224T3 - Subtilisinas con mutaciones multiples. - Google Patents
Subtilisinas con mutaciones multiples.Info
- Publication number
- ES2091224T3 ES2091224T3 ES90304715T ES90304715T ES2091224T3 ES 2091224 T3 ES2091224 T3 ES 2091224T3 ES 90304715 T ES90304715 T ES 90304715T ES 90304715 T ES90304715 T ES 90304715T ES 2091224 T3 ES2091224 T3 ES 2091224T3
- Authority
- ES
- Spain
- Prior art keywords
- subtilisins
- multiple mutations
- subtilisin
- admixture
- expressing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Cosmetics (AREA)
- Tires In General (AREA)
Abstract
UNA CLASE DE ANALOGOS DE LA SUBTILISINA ADECUADA PARA SER MEZCLADA CON COMPOSICIONES DE LIMPIEZA Y CON UNA ESTABILIDAD MEJORADA SOBRE EL BACILO DE LA SUBTISILINA. ESTOS ANALOGOS SON PREPARADOS EXPRESANDO UN GEN MODIFICADO CODIFICADOR DEL ANALOGO DE LA SUBTILISINA EN EL BACILLUS SUBTILIS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35312489A | 1989-05-17 | 1989-05-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2091224T3 true ES2091224T3 (es) | 1996-11-01 |
Family
ID=23387862
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90304715T Expired - Lifetime ES2091224T3 (es) | 1989-05-17 | 1990-05-01 | Subtilisinas con mutaciones multiples. |
Country Status (14)
Country | Link |
---|---|
US (1) | US5397705A (es) |
EP (1) | EP0398539B1 (es) |
JP (1) | JPH03505976A (es) |
KR (1) | KR100188532B1 (es) |
AT (1) | ATE141324T1 (es) |
AU (1) | AU618675B2 (es) |
CA (1) | CA2016211C (es) |
DE (1) | DE69028040T2 (es) |
DK (1) | DK0398539T3 (es) |
ES (1) | ES2091224T3 (es) |
GR (1) | GR3021010T3 (es) |
HK (1) | HK1006857A1 (es) |
SG (1) | SG47797A1 (es) |
WO (1) | WO1990014420A1 (es) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3856362T2 (de) * | 1987-04-06 | 2000-05-18 | Novo Nordisk A/S, Bagsvaerd | Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
US5863783A (en) * | 1991-03-27 | 1999-01-26 | Gist-Brocades, N.V. | Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin |
WO1993008253A1 (en) * | 1991-10-16 | 1993-04-29 | Unilever N.V. | Aqueous enzymatic detergent compositions |
US5989887A (en) * | 1992-11-25 | 1999-11-23 | Dsm N.V. | Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin |
US5567601A (en) * | 1993-06-01 | 1996-10-22 | University Of Maryland | Subtilisin mutants lacking a primary calcium binding site |
US6440717B1 (en) | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
US6436690B1 (en) | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
MA23346A1 (fr) | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
DE69434962T2 (de) * | 1993-10-14 | 2008-01-17 | The Procter & Gamble Company, Cincinnati | Proteasehaltige reinigungsmittel |
CZ105296A3 (en) * | 1993-10-14 | 1996-09-11 | Procter & Gamble | Bleaching agent containing protease enzymes, cleansing agents, agent for cleaning fabrics and fabric cleaning method |
DE4411223A1 (de) * | 1994-03-31 | 1995-10-05 | Solvay Enzymes Gmbh & Co Kg | Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren |
US6599730B1 (en) | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US6066611A (en) * | 1994-10-13 | 2000-05-23 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
US6455295B1 (en) | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
DE69720043T2 (de) * | 1996-12-20 | 2003-10-16 | Unilever N.V., Rotterdam | Enzymatische bleichmittelzusammensetzung |
US6204234B1 (en) * | 1997-07-09 | 2001-03-20 | The Proctor & Gamble Company | Cleaning compositions comprising a specific oxygenase |
AR015977A1 (es) * | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
ES2368718T3 (es) * | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
US6908757B1 (en) | 1998-03-26 | 2005-06-21 | The Procter & Gamble Company | Serine protease variants having amino acid deletions and substitutions |
WO1999049056A1 (en) * | 1998-03-26 | 1999-09-30 | The Procter & Gamble Company | Serine protease variants having amino acid substitutions |
MXPA02000842A (es) | 1999-07-22 | 2002-07-30 | Procter & Gamble | Conjugados de proteasa que tienen sitios de corte protegidos estericamente. |
CZ2002212A3 (cs) | 1999-07-22 | 2002-07-17 | The Procter & Gamble Company | Varianty subtilisinové proteasy mající aminokyselinové substituce v definovaných epitopových oblastech |
US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
CN1373802A (zh) | 1999-07-22 | 2002-10-09 | 宝洁公司 | 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体 |
US7217554B2 (en) | 1999-08-31 | 2007-05-15 | Novozymes A/S | Proteases and variants thereof |
NZ517409A (en) | 1999-08-31 | 2004-05-28 | Novozymes As | RP-II properties with amino acid substitutions used in detergent compositions and additives |
US6727085B2 (en) * | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
AU2001280968A1 (en) * | 2000-07-31 | 2002-02-13 | Menzel, Rolf | Compositions and methods for directed gene assembly |
HUP0303199A2 (hu) | 2001-02-19 | 2003-12-29 | Merck Patent Gmbh | Eljárás T-sejt-epitópok azonosítására és csökkentett immunogenitású molekulák előállítására |
CA2441595C (en) * | 2001-03-23 | 2012-07-03 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
CA2455607C (en) * | 2001-07-09 | 2013-01-08 | Children's Hospital Medical Center Of Akron | Multiple controls for molecular genetic analyses |
DE10153792A1 (de) | 2001-10-31 | 2003-05-22 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
JP5328002B2 (ja) * | 2002-01-16 | 2013-10-30 | ジェネンコー・インターナショナル・インク | 複数置換プロテアーゼ変異体 |
US7888093B2 (en) * | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
US7294499B2 (en) | 2003-01-30 | 2007-11-13 | Novozymes A/S | Subtilases |
US7727756B2 (en) * | 2003-03-21 | 2010-06-01 | Novozymes A/S | Subtilases |
BRPI0409992A (pt) * | 2003-05-07 | 2006-05-09 | Novozymes As | enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar |
US20230028935A1 (en) * | 2018-11-28 | 2023-01-26 | Danisco Us Inc | Subtilisin variants having improved stability |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5155033A (en) * | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
WO1987004461A1 (en) * | 1986-01-15 | 1987-07-30 | Amgen | THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF |
US4990452A (en) * | 1986-02-12 | 1991-02-05 | Genex Corporation | Combining mutations for stabilization of subtilisin |
US4980288A (en) * | 1986-02-12 | 1990-12-25 | Genex Corporation | Subtilisin with increased thermal stability |
US5013657A (en) * | 1988-04-12 | 1991-05-07 | Bryan Philip N | Subtilisin mutations |
SG30639G (en) * | 1986-04-30 | 1995-09-01 | Genencor Int | Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors |
NZ221627A (en) * | 1986-09-09 | 1993-04-28 | Genencor Inc | Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios |
EP0896062A3 (en) * | 1987-02-27 | 1999-04-07 | Genencor International, Inc. | Transformation of alkalophilic bacillus strains |
DE3856362T2 (de) * | 1987-04-06 | 2000-05-18 | Novo Nordisk A/S, Bagsvaerd | Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen |
US4914031A (en) * | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
FR2614467A1 (fr) * | 1987-04-21 | 1988-10-28 | Telemecanique Electrique | Appareil interrupteur de protection a mecanisme de declenchement simplifie |
-
1990
- 1990-04-23 JP JP2507107A patent/JPH03505976A/ja active Pending
- 1990-04-23 WO PCT/US1990/002234 patent/WO1990014420A1/en unknown
- 1990-04-23 KR KR1019910700067A patent/KR100188532B1/ko not_active IP Right Cessation
- 1990-04-23 AU AU55580/90A patent/AU618675B2/en not_active Expired
- 1990-05-01 EP EP90304715A patent/EP0398539B1/en not_active Expired - Lifetime
- 1990-05-01 DK DK90304715.7T patent/DK0398539T3/da active
- 1990-05-01 ES ES90304715T patent/ES2091224T3/es not_active Expired - Lifetime
- 1990-05-01 DE DE69028040T patent/DE69028040T2/de not_active Expired - Lifetime
- 1990-05-01 AT AT90304715T patent/ATE141324T1/de not_active IP Right Cessation
- 1990-05-01 SG SG1996004414A patent/SG47797A1/en unknown
- 1990-05-07 CA CA002016211A patent/CA2016211C/en not_active Expired - Lifetime
-
1992
- 1992-03-25 US US07/857,714 patent/US5397705A/en not_active Expired - Lifetime
-
1996
- 1996-09-12 GR GR960402360T patent/GR3021010T3/el unknown
-
1998
- 1998-06-23 HK HK98106058A patent/HK1006857A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR100188532B1 (ko) | 1999-06-01 |
CA2016211C (en) | 1998-07-21 |
AU618675B2 (en) | 1992-01-02 |
WO1990014420A1 (en) | 1990-11-29 |
DK0398539T3 (da) | 1996-12-09 |
EP0398539A1 (en) | 1990-11-22 |
KR920701426A (ko) | 1992-08-11 |
DE69028040D1 (de) | 1996-09-19 |
SG47797A1 (en) | 1998-04-17 |
US5397705A (en) | 1995-03-14 |
ATE141324T1 (de) | 1996-08-15 |
JPH03505976A (ja) | 1991-12-26 |
AU5558090A (en) | 1990-12-18 |
EP0398539B1 (en) | 1996-08-14 |
CA2016211A1 (en) | 1990-11-17 |
DE69028040T2 (de) | 1997-02-27 |
HK1006857A1 (en) | 1999-03-19 |
GR3021010T3 (en) | 1996-12-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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