DD229151A5 - Verfahren zur herstellung von gewebeplasminogenaktivatoren - Google Patents

Verfahren zur herstellung von gewebeplasminogenaktivatoren Download PDF

Info

Publication number
DD229151A5
DD229151A5 DD84269628A DD26962884A DD229151A5 DD 229151 A5 DD229151 A5 DD 229151A5 DD 84269628 A DD84269628 A DD 84269628A DD 26962884 A DD26962884 A DD 26962884A DD 229151 A5 DD229151 A5 DD 229151A5
Authority
DD
German Democratic Republic
Prior art keywords
gpa
yeast
dna
pho5
promoter
Prior art date
Application number
DD84269628A
Other languages
German (de)
English (en)
Inventor
Bernd Meyhack
Albert Hinnen
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB838331045A external-priority patent/GB8331045D0/en
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of DD229151A5 publication Critical patent/DD229151A5/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DD84269628A 1983-11-21 1984-11-19 Verfahren zur herstellung von gewebeplasminogenaktivatoren DD229151A5 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB838331045A GB8331045D0 (en) 1983-11-21 1983-11-21 Synthesis of fibrinolytic agents by yeast
GB848423112A GB8423112D0 (en) 1983-11-21 1984-09-13 Synthesis of fibrino lytic agents by yeast

Publications (1)

Publication Number Publication Date
DD229151A5 true DD229151A5 (de) 1985-10-30

Family

ID=26287024

Family Applications (1)

Application Number Title Priority Date Filing Date
DD84269628A DD229151A5 (de) 1983-11-21 1984-11-19 Verfahren zur herstellung von gewebeplasminogenaktivatoren

Country Status (17)

Country Link
EP (1) EP0143081B1 (xx)
JP (1) JPS6119489A (xx)
KR (1) KR930002889B1 (xx)
AU (1) AU595478B2 (xx)
DD (1) DD229151A5 (xx)
DE (1) DE3479520D1 (xx)
DK (1) DK164941C (xx)
ES (1) ES8609471A1 (xx)
FI (1) FI86746C (xx)
GR (1) GR80966B (xx)
HU (1) HU201115B (xx)
IE (1) IE57699B1 (xx)
IL (1) IL73568A (xx)
NO (1) NO844618L (xx)
NZ (1) NZ210266A (xx)
PH (1) PH22337A (xx)
PT (1) PT79518B (xx)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
US7138505B1 (en) 1984-01-12 2006-11-21 Novartis Vaccines And Diagnostics, Inc. Factor VIII:C nucleic acid molecules
JPS61502655A (ja) * 1984-07-09 1986-11-20 インテグレ−テッド・ジェネティックス・インコ−ポレ−テッド 酵母クロ−ニングビヒクル
IE81135B1 (en) * 1984-10-01 2000-03-22 Genzyme Corp Recombinant DNA techniques and the products thereof
JPS624233A (ja) * 1985-07-01 1987-01-10 Toyobo Co Ltd 組織性プラスミノ−ゲン活性化因子の製造法
JPS6229975A (ja) * 1985-08-01 1987-02-07 Mitsui Toatsu Chem Inc 粗ｔＰＡの精製法
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
DE3675724D1 (de) * 1985-09-18 1991-01-03 Green Cross Corp Hefe-promotor und verfahren zur herstellung von heterologen proteinen.
USH2055H1 (en) * 1985-09-20 2002-12-03 Zymogenetics, Inc. Expression of tissue-type plasminogen activator in bacteria
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
US5106741A (en) * 1985-12-20 1992-04-21 The Upjohn Company Tissue plasminogen activator (TPA) analogs
US4898825A (en) * 1986-05-07 1990-02-06 Mitsui Toatsu Chemicals, Incorporated Methods for purification of single-chain and double-chain tissue plasminogen activator
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
PT84991B (pt) * 1986-06-06 1990-03-08 Genentech Inc Processo para a producao de actividador do plasminogenio biologicamente activo
JPS6463378A (en) * 1986-08-11 1989-03-09 Mitsui Toatsu Chemicals Separation of single stranded tpa and double standard tpa
GB8621118D0 (en) * 1986-09-01 1986-10-08 Whitbread & Co Plc Stabilised polypeptides
EP0263072B1 (en) 1986-10-03 1994-03-23 Ciba-Geigy Ag Novel lymphokine related peptides
PT86162B (pt) * 1986-11-21 1990-11-20 Smithkline Beecham Corp Expressao de proteina recombinante
ZA879286B (en) 1986-12-16 1988-10-26 Smith Kline Rit New plasminogen activators
US5149533A (en) * 1987-06-04 1992-09-22 Zymogenetics, Inc. Modified t-PA with kringle-/replaced by another kringle
US5089409A (en) * 1989-09-28 1992-02-18 Monsanto Company Method of increasing specific activity of t-pa
US5326700A (en) * 1990-11-06 1994-07-05 Eli Lilly And Company DNA sequences encoding t-PA derivatives with cleavable sites
JPH08502661A (ja) * 1993-08-04 1996-03-26 チバ−ガイギー アクチェンゲゼルシャフト 高分子量デスルファトヒルジン
JP3732516B2 (ja) 1995-02-09 2006-01-05 ノバルティス・アクチエンゲゼルシャフト タンパク質の製造法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
GR79202B (xx) * 1982-05-05 1984-10-22 Genentech Inc
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
EP0174835A1 (en) * 1984-09-11 1986-03-19 The Upjohn Company Human tissue plasminogen activator and recombinant DNA compounds
IE81135B1 (en) * 1984-10-01 2000-03-22 Genzyme Corp Recombinant DNA techniques and the products thereof

Also Published As

Publication number Publication date
IE842965L (en) 1985-05-21
DK164941B (da) 1992-09-14
ES8609471A1 (es) 1986-07-16
IL73568A (en) 1992-07-15
DE3479520D1 (en) 1989-09-28
ES537828A0 (es) 1986-07-16
JPS6119489A (ja) 1986-01-28
EP0143081A2 (en) 1985-05-29
IE57699B1 (en) 1993-03-10
KR930002889B1 (ko) 1993-04-12
GR80966B (en) 1985-03-15
FI86746B (fi) 1992-06-30
PH22337A (en) 1988-08-12
AU595478B2 (en) 1990-04-05
KR850003733A (ko) 1985-06-26
PT79518A (en) 1984-12-01
FI844544L (fi) 1985-05-22
HUT36185A (en) 1985-08-28
EP0143081B1 (en) 1989-08-23
NO844618L (no) 1985-05-22
DK164941C (da) 1993-02-01
FI844544A0 (fi) 1984-11-19
FI86746C (fi) 1992-10-12
PT79518B (pt) 1986-12-12
DK551084D0 (da) 1984-11-20
DK551084A (da) 1985-05-22
NZ210266A (en) 1988-11-29
IL73568A0 (en) 1985-02-28
EP0143081A3 (en) 1987-01-07
AU3571984A (en) 1986-03-20
HU201115B (en) 1990-09-28

Similar Documents

Publication Publication Date Title
DD229151A5 (de) Verfahren zur herstellung von gewebeplasminogenaktivatoren
DE3316297C2 (xx)
DE69434332T2 (de) Thrombin mutanten
DD218118A5 (de) Verfahren zur herstellung eines hefe- oder nichthefepolypeptids
DD296965A5 (de) Hefeverarbeitungssystem
WO2003066842A2 (de) Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen
DE3537176C2 (de) Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung
EP0408945B1 (de) Plasmide, deren Herstellung und deren Verwendung bei der Gewinnung eines Plasminogenaktivators
DD274053A5 (de) Verfahren zur Produktion eines menschlichen, einkettigen Plasminogenaktivators
EP0496327B1 (de) Verfahren zur Herstellung von Polypeptiden mit Prourokinase-Aktivität
WO1996001312A1 (de) Nicht-glykosylierte plasminogenaktivator-derivate und ihre verwendung bei erhöhtem blutungsrisiko
WO1992004444A1 (en) Methods for producing acyloxyacyl hydrolase
EP1169461A2 (de) Herstellung von pankreatischer procarboxypeptidase b, isoformen und muteinen davon und ihre verwendung
EP0300425A2 (de) Verfahren zur Herstellung von Proteinen in löslicher Form
WO1998005762A1 (de) Thrombinaktivierbarer plasminogenaktivator
WO1987006611A1 (en) Process for producing plasminogen-activators in procaryotes
JPS62500103A (ja) ヒト結合組織アクチベ−タ−及び関連ペプチドの発現
DE3348289C2 (de) Verfahren zur Herstellung von Human-Gewebe-Plasminogen-Aktivator
DD231372A5 (de) Herstellung funktioneller menschlicher urokinaseproteine
DD260293A5 (de) Gewebs-Plasminogen-Aktivator (Tpa) - Derivat und seine Herstellung
DD283839A5 (de) Verfahren zur herstellung eines hefestammes ohne carboxyteptidase-ysc alpha-aktivitaet
WO1990000603A1 (de) Neue polynukleotidsequenzen
DD255170A5 (de) Verfahren zur Herstellung von proteinen mit Hirudinaktivität
EP0312941A2 (de) Polypeptide mit Prourokinase-Aktivität, ihre Herstellung und Verwendung
DD257645A5 (de) Verfahren zur Herstellung eines Plasminogen-Aktivator

Legal Events

Date Code Title Description
ENJ Ceased due to non-payment of renewal fee