ES8609471A1 - Procedimiento para preparar agentes fibrinoliticos - Google Patents

Procedimiento para preparar agentes fibrinoliticos

Info

Publication number
ES8609471A1
ES8609471A1 ES537828A ES537828A ES8609471A1 ES 8609471 A1 ES8609471 A1 ES 8609471A1 ES 537828 A ES537828 A ES 537828A ES 537828 A ES537828 A ES 537828A ES 8609471 A1 ES8609471 A1 ES 8609471A1
Authority
ES
Spain
Prior art keywords
tissue plasminogen
yeast
plasminogen activator
tpa
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES537828A
Other languages
English (en)
Other versions
ES537828A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB838331045A external-priority patent/GB8331045D0/en
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Publication of ES8609471A1 publication Critical patent/ES8609471A1/es
Publication of ES537828A0 publication Critical patent/ES537828A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR AGENTES FIBRINOLITICOS. COMPRENDE: A) CULTIVAR UNA CEPA DE LEVADURA TRANSFORMADA CON UN VECTOR HIBRIDO QUE CONTIENE UN PROMOTOR DE LEVADURA Y UNA REGION DE CODIFICACION DE TPA QUE ES CONTROLADA POR DICHO PROMOTOR, EN UN MEDIO LIQUIDO CONTENIENDO FUENTES ASIMILABLES DE CARBONO, NITROGENO Y SALES INORGANICAS, EN CULTIVO SUMERGIDO CON AGITACION A UNA TEMPERATURA DE 25 A 35JC, A UN PH DE 4 A 8 Y DURANTE UN TIEMPO DE 4 A 20 HORAS, PARA CONSEGUIR LOS MAXIMOS RENDIMIENTOS DE PROTEINAS; Y B) AISLAR Y PURIFICAR DICHAS PROTEINAS, SEPARANDO PRIMERAMENTE LA PARED CELULAR POR DIGESTION ENZIMATICA CON GLUCOSIDADES Y LOS ESFEROPLASTOS RESULTANTES SE TRATAN CON DETERGENTES, COMO TRITON, LA MEZCLA PROTEINICA RESULTANTE SE ENRIQUECE EN TPA POR MEDIOS CONVENCIONALES Y LA PURIFICACION FINAL SE CONSIGUE POR CROMATOGRAFIA POR AFINIDAD. SE UTILIZAN PARA LA PREVENCION O TRATAMIENTO DE LA TROMBOSIS.
ES537828A 1983-11-21 1984-11-21 Procedimiento para preparar agentes fibrinoliticos Expired ES8609471A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB838331045A GB8331045D0 (en) 1983-11-21 1983-11-21 Synthesis of fibrinolytic agents by yeast
GB848423112A GB8423112D0 (en) 1983-11-21 1984-09-13 Synthesis of fibrino lytic agents by yeast

Publications (2)

Publication Number Publication Date
ES8609471A1 true ES8609471A1 (es) 1986-07-16
ES537828A0 ES537828A0 (es) 1986-07-16

Family

ID=26287024

Family Applications (1)

Application Number Title Priority Date Filing Date
ES537828A Expired ES8609471A1 (es) 1983-11-21 1984-11-21 Procedimiento para preparar agentes fibrinoliticos

Country Status (17)

Country Link
EP (1) EP0143081B1 (es)
JP (1) JPS6119489A (es)
KR (1) KR930002889B1 (es)
AU (1) AU595478B2 (es)
DD (1) DD229151A5 (es)
DE (1) DE3479520D1 (es)
DK (1) DK164941C (es)
ES (1) ES8609471A1 (es)
FI (1) FI86746C (es)
GR (1) GR80966B (es)
HU (1) HU201115B (es)
IE (1) IE57699B1 (es)
IL (1) IL73568A (es)
NO (1) NO844618L (es)
NZ (1) NZ210266A (es)
PH (1) PH22337A (es)
PT (1) PT79518B (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
US7138505B1 (en) 1984-01-12 2006-11-21 Novartis Vaccines And Diagnostics, Inc. Factor VIII:C nucleic acid molecules
JPS61502655A (ja) * 1984-07-09 1986-11-20 インテグレ−テッド・ジェネティックス・インコ−ポレ−テッド 酵母クロ−ニングビヒクル
IE81135B1 (en) * 1984-10-01 2000-03-22 Genzyme Corp Recombinant DNA techniques and the products thereof
JPS624233A (ja) * 1985-07-01 1987-01-10 Toyobo Co Ltd 組織性プラスミノ−ゲン活性化因子の製造法
JPS6229975A (ja) * 1985-08-01 1987-02-07 Mitsui Toatsu Chem Inc 粗ｔＰＡの精製法
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
DE3675724D1 (de) * 1985-09-18 1991-01-03 Green Cross Corp Hefe-promotor und verfahren zur herstellung von heterologen proteinen.
USH2055H1 (en) * 1985-09-20 2002-12-03 Zymogenetics, Inc. Expression of tissue-type plasminogen activator in bacteria
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
US5106741A (en) * 1985-12-20 1992-04-21 The Upjohn Company Tissue plasminogen activator (TPA) analogs
US4898825A (en) * 1986-05-07 1990-02-06 Mitsui Toatsu Chemicals, Incorporated Methods for purification of single-chain and double-chain tissue plasminogen activator
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
PT84991B (pt) * 1986-06-06 1990-03-08 Genentech Inc Processo para a producao de actividador do plasminogenio biologicamente activo
JPS6463378A (en) * 1986-08-11 1989-03-09 Mitsui Toatsu Chemicals Separation of single stranded tpa and double standard tpa
GB8621118D0 (en) * 1986-09-01 1986-10-08 Whitbread & Co Plc Stabilised polypeptides
EP0263072B1 (en) 1986-10-03 1994-03-23 Ciba-Geigy Ag Novel lymphokine related peptides
PT86162B (pt) * 1986-11-21 1990-11-20 Smithkline Beecham Corp Expressao de proteina recombinante
ZA879286B (en) 1986-12-16 1988-10-26 Smith Kline Rit New plasminogen activators
US5149533A (en) * 1987-06-04 1992-09-22 Zymogenetics, Inc. Modified t-PA with kringle-/replaced by another kringle
US5089409A (en) * 1989-09-28 1992-02-18 Monsanto Company Method of increasing specific activity of t-pa
US5326700A (en) * 1990-11-06 1994-07-05 Eli Lilly And Company DNA sequences encoding t-PA derivatives with cleavable sites
JPH08502661A (ja) * 1993-08-04 1996-03-26 チバ−ガイギー アクチェンゲゼルシャフト 高分子量デスルファトヒルジン
JP3732516B2 (ja) 1995-02-09 2006-01-05 ノバルティス・アクチエンゲゼルシャフト タンパク質の製造法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
GR79202B (es) * 1982-05-05 1984-10-22 Genentech Inc
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
EP0174835A1 (en) * 1984-09-11 1986-03-19 The Upjohn Company Human tissue plasminogen activator and recombinant DNA compounds
IE81135B1 (en) * 1984-10-01 2000-03-22 Genzyme Corp Recombinant DNA techniques and the products thereof

Also Published As

Publication number Publication date
IE842965L (en) 1985-05-21
DK164941B (da) 1992-09-14
IL73568A (en) 1992-07-15
DE3479520D1 (en) 1989-09-28
ES537828A0 (es) 1986-07-16
JPS6119489A (ja) 1986-01-28
EP0143081A2 (en) 1985-05-29
IE57699B1 (en) 1993-03-10
KR930002889B1 (ko) 1993-04-12
GR80966B (en) 1985-03-15
FI86746B (fi) 1992-06-30
PH22337A (en) 1988-08-12
AU595478B2 (en) 1990-04-05
KR850003733A (ko) 1985-06-26
PT79518A (en) 1984-12-01
FI844544L (fi) 1985-05-22
HUT36185A (en) 1985-08-28
EP0143081B1 (en) 1989-08-23
DD229151A5 (de) 1985-10-30
NO844618L (no) 1985-05-22
DK164941C (da) 1993-02-01
FI844544A0 (fi) 1984-11-19
FI86746C (fi) 1992-10-12
PT79518B (pt) 1986-12-12
DK551084D0 (da) 1984-11-20
DK551084A (da) 1985-05-22
NZ210266A (en) 1988-11-29
IL73568A0 (en) 1985-02-28
EP0143081A3 (en) 1987-01-07
AU3571984A (en) 1986-03-20
HU201115B (en) 1990-09-28

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Legal Events

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FD1A Patent lapsed

Effective date: 19970519