KR850003733A - 효모에 의한 조직 플라스미노겐 활성화제의 제조방법 - Google Patents

효모에 의한 조직 플라스미노겐 활성화제의 제조방법 Download PDF

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KR850003733A
KR850003733A KR1019840007284A KR840007284A KR850003733A KR 850003733 A KR850003733 A KR 850003733A KR 1019840007284 A KR1019840007284 A KR 1019840007284A KR 840007284 A KR840007284 A KR 840007284A KR 850003733 A KR850003733 A KR 850003733A
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yeast
plasminogen activator
tissue plasminogen
hybrid vector
tpa
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메이 헥 베른드 (외 1)
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아놀드 자일러, 에른스트 알테르
시바-가이기 에이지
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Abstract

내용 없음

Description

효모에 의한 조직 플라스미노겐 활성화제의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 pH05 유전자 또는 DNA서열의 재료로 각각 사용되는 플라스미드 pJDB207/pH05, pH03 및 pBR322/pH05 Bam-Sal의 부분제한 엔도뉴클레아제 지도에 관한 것이다.
제2도는 5.1kb BamHI 단퍼이 효모유전자에서 단리된 pH05 및 pH03산 포스파타아제 유전자의 퍼재(localization)에 관한 것이다.
제3a도 및 3b도는 각각 pH05 및 pH03의 프로모터 부위의 DNA서열에 관한 것이다.

Claims (20)

  1. 적절한 영양조건하에서 효모 프로모터와 이 프로모터에 의해 조절되는 조직 플라스미노겐 활성화제(Tissue plasminogen activator, TPA) 코드화부위를 함유하는 하이브리드 벡터로 형질전환된 효모균주, 효모염색체에 안정하게 결합되고 염색체상 효모프로모터의 조절하에 있는 조직플라스미노겐 활성화제에 대한 유전자를 함유하는 효모균주 또는 상기 효모균주의 돌연변이체를 배양시키고, 필요하면, 수득된 조직플라-스미노겐 활성화제(TPA) 및 전-조직 플라스미노겐 활성화제(프로-TPA)의 혼합물을 개별 성분으로 분리시키고, 프로-TPA를 함유하지 않은 TPA를 생성하기 위해 생성된 프로-TPA를 TPA로 전환시키고, 필요하면, 혼합물중에서 수득된 글리코실화 생성물과 비글리코실화 생성물을 분리시키고, 글리코실화 단백질을 생성하기 위해 수득된 단백질에서 글리코실 잔기를 제거함을 특징으로 하여 글리코실화 또는 비글리코실화 형태 또는 이들의 혼합물 형태인 조직플라스미노겐 활성화제(TPA), 전-조직 플라스미노겐 활성화제(프로-TPA) 또는 그의 혼합물을 제조하는 방법.
  2. 제1항에 있어서, 효모 하이브리드 벡터가 인체 색소세포종 세포, 섬유육종, 피부암세포, 방광암세포, 부신종양세포, 지방육종, 헬라세포, 섬유아세포, 케라토사이트, 유방 상피 세포 및 신장 세포중에서 유도된 조직 플라스미노겐 활성화제의 코드화부위를 함유함을 특징으로 하는 방법.
  3. 제1항에 있어서, 효모 하이브리드 벡터가 헬라 세포로부터 유도된 조직플라스미노겐 활성화제 코드화부위를 함유함을 특징으로 하는 방법.
  4. 제1항에 있어서, 효모 하이브리드 벡터가 외인적으로 조절될 수 있는 효모 프로모터를 함유함을 특징으로 하는 방법.
  5. 제1항에 있어서, 효모 하이브리드 벡터가 효모pH05프로모터를 함유함을 특징으로 하는 방법.
  6. 제1항에 있어서, 효모 하이브리드 벡터가 접합부위에 삽입된 ATG해독 개시 시그널과 함께 숙성조직 플라스미노겐 활성화제의 코드화부위에 직접 결합된 효모 프로모터를 함유함을 특징으로 하는 방법.
  7. 제1항에 있어서, 효모 하이브리드 벡터가 시그널 서열 다음에 조직 플라스미노겐 활성화제 코드화 부위를 함유함을 특징으로 하는 방법.
  8. 제1항에 있어서, 효모 하이브리드 벡터가pH05시그널 서열 다음에 조직 플라스미노겐 활성화제 코드화부위를 함유함을 특징으로 하는 방법.
  9. 제1항에 있어서, 효모 하이브리드 벡터가 조직 플라스미노겐 활성화제 프레프로-서열 다음에 조직 플라스미노겐 활성화제 코드화 부위를 함유함을 특징으로 하는 방법.
  10. 제1항에 있어서, 효모 하이브리드 벡터가 프로모터에 의해 천연적으로 조절된 유전자의 코드부위에 연장되고 효모 엔드펩티다아제에 대한 진행부위를 함유하는 합성 결합제(linker)를 통해 조직 플라스미노겐 활성화제 코드화부위에 연결된 효모 프로모터를 함유함을 특징으로 하는 방법.
  11. 제1항에 있어서, 효모 하이브리드 벡터가 효모프로모터 및pH05유전자로부터 유도된 연장된 시그널 서열을 함유함을 특징으로 하는 방법.
  12. 제1항에 있어서, 조직 플라스미노겐 활성화제 전사가 효모 유전자의 전사 종결 시그널을 함유하는 DNA 서열에서 종결됨을 특징으로 하는 방법.
  13. 제1항에 있어서, 효모가시카로마이세스 세레비지아에(saccharomyces cerevisiae)인 방법.
  14. 제1항에 있어서, 효모 균주를 탄소, 질소 및 무기염 동화원을 함유하는 액체 배지중에서 배양시키는 방법.
  15. 제1항에 있어서, 조직 플라스미노겐 활성화제, 전-조직 플라스미노겐 활성화제 및 그의 혼합물을 DE-3 세파로즈 컬럼 상에서 선택적 흡착에 의해 배양액에서 분리시키는 방법.
  16. 제1항에 있어서, 조직 플라스미노겐 활성화제, 전-조직 플라스미노겐 활성화제 및 그의 혼합물을 모노-클로날 항체를 사용하여친화 크로마토그라피시켜 배양액에서 분리시키는 방법.
  17. 제1항에 있어서, 수득된 전-조직 플라-스미노겐 활성화제를 효소적으로 조직 플라스미노겐 활성화제로 전환시키는 방법.
  18. 제1항에 있어서, 조직 플라스미노겐 활성화제를 거의 함유하지 않는 전-조직 플라스미노겐 활성화제를 제조하기 위해, 정제 공정을 수행하는 동안 프로테아제 억제제를 함유시키고 DE-3 세파로즈 컬럼상의 크로마토-그라피를 조직 플라스미노겐 활성화제에만 선택적으로 결합하는 억제제의 존재하에 수행하는 방법.
  19. 제1항에 있어서, 수득된 글리코실화 및 비글리코실화 생성물을 콘카나발린-A 세파로즈 컬럼상의 크로마토그라피에 의해 분리하는 방법.
  20. 제1항에 있어서, 효모특이성 글리코실화를 함유하는 조직 플라스미노겐 활성화제, 전-조직 플라스미노겐 활성화제 및 그의 혼합물의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840007284A 1983-11-21 1984-11-21 효모에 의한 조직 플라스미노겐 활성화제의 제조방법 KR930002889B1 (ko)

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GB838331045A GB8331045D0 (en) 1983-11-21 1983-11-21 Synthesis of fibrinolytic agents by yeast
GB8331045 1983-11-21
GB8423112 1984-09-13
GB848423112A GB8423112D0 (en) 1983-11-21 1984-09-13 Synthesis of fibrino lytic agents by yeast

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KR850003733A true KR850003733A (ko) 1985-06-26
KR930002889B1 KR930002889B1 (ko) 1993-04-12

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JPS624233A (ja) * 1985-07-01 1987-01-10 Toyobo Co Ltd 組織性プラスミノ−ゲン活性化因子の製造法
JPS6229975A (ja) * 1985-08-01 1987-02-07 Mitsui Toatsu Chem Inc 粗ｔＰＡの精製法
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PH22337A (en) 1988-08-12
PT79518A (en) 1984-12-01
DK164941C (da) 1993-02-01
FI844544A0 (fi) 1984-11-19
HU201115B (en) 1990-09-28
IE842965L (en) 1985-05-21
EP0143081A2 (en) 1985-05-29
IL73568A (en) 1992-07-15
JPS6119489A (ja) 1986-01-28
KR930002889B1 (ko) 1993-04-12
ES537828A0 (es) 1986-07-16
EP0143081B1 (en) 1989-08-23
IE57699B1 (en) 1993-03-10
GR80966B (en) 1985-03-15
PT79518B (pt) 1986-12-12
DK551084D0 (da) 1984-11-20
DE3479520D1 (en) 1989-09-28
DK551084A (da) 1985-05-22
FI86746B (fi) 1992-06-30
FI844544L (fi) 1985-05-22
DD229151A5 (de) 1985-10-30
DK164941B (da) 1992-09-14
NZ210266A (en) 1988-11-29
FI86746C (fi) 1992-10-12
EP0143081A3 (en) 1987-01-07
ES8609471A1 (es) 1986-07-16
IL73568A0 (en) 1985-02-28
NO844618L (no) 1985-05-22
AU595478B2 (en) 1990-04-05
AU3571984A (en) 1986-03-20
HUT36185A (en) 1985-08-28

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