CR20190130A - Métodos y composiciones para plantas de estatura corta a través de la manipulación del metabolismo de giberelina para aumentar el rendimiento cosechable - Google Patents
Métodos y composiciones para plantas de estatura corta a través de la manipulación del metabolismo de giberelina para aumentar el rendimiento cosechableInfo
- Publication number
- CR20190130A CR20190130A CR20190130A CR20190130A CR20190130A CR 20190130 A CR20190130 A CR 20190130A CR 20190130 A CR20190130 A CR 20190130A CR 20190130 A CR20190130 A CR 20190130A CR 20190130 A CR20190130 A CR 20190130A
- Authority
- CR
- Costa Rica
- Prior art keywords
- gibberellin
- methods
- compositions
- plants
- expression
- Prior art date
Links
- 229930191978 Gibberellin Natural products 0.000 title abstract 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 title abstract 5
- 239000003448 gibberellin Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 5
- 208000020221 Short stature Diseases 0.000 title 1
- 230000004060 metabolic process Effects 0.000 title 1
- 241000196324 Embryophyta Species 0.000 abstract 8
- 230000001629 suppression Effects 0.000 abstract 4
- 101100121112 Oryza sativa subsp. indica 20ox2 gene Proteins 0.000 abstract 2
- 101100121113 Oryza sativa subsp. japonica GA20OX2 gene Proteins 0.000 abstract 2
- 108090000854 Oxidoreductases Proteins 0.000 abstract 2
- 241000209149 Zea Species 0.000 abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 235000013339 cereals Nutrition 0.000 abstract 2
- 235000005822 corn Nutrition 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract 2
- 238000002703 mutagenesis Methods 0.000 abstract 2
- 231100000350 mutagenesis Toxicity 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8225—Leaf-specific, e.g. including petioles, stomata
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8291—Hormone-influenced development
- C12N15/8297—Gibberellins; GA3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Abstract
La presente invención se refiere a composiciones y métodos para alterar el contenido de giberelina (GA) en plantas de maíz u otro cereal. También se proporcionan métodos y composiciones para alterar la expresión de genes relacionados con la biosíntesis de la giberelina mediante la supresión, mutagenésis y/o edición de los subtipos específicos del gen de GA20 o GA3 oxidasas. Se proporcionan adicionalmente plantas y células de plantas modificadas que tienen un elemento de supresión o mutación que redice la expresión o actividad de un gen de GA oxidasa que comprenden niveles de giberelina reducidos y características mejoradas, tales como altura reducida de la planta y resistencia al encamado aumentada, pero sin fueras de tipo.The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US201662376298P | 2016-08-17 | 2016-08-17 | |
US201762442377P | 2017-01-04 | 2017-01-04 | |
US201762502313P | 2017-05-05 | 2017-05-05 | |
PCT/US2017/047405 WO2018035354A1 (en) | 2016-08-17 | 2017-08-17 | Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield |
Publications (1)
Publication Number | Publication Date |
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CR20190130A true CR20190130A (es) | 2019-05-07 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CR20190130A CR20190130A (es) | 2016-08-17 | 2017-08-17 | Métodos y composiciones para plantas de estatura corta a través de la manipulación del metabolismo de giberelina para aumentar el rendimiento cosechable |
Country Status (15)
Country | Link |
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US (5) | US10724047B2 (es) |
EP (1) | EP3500666A4 (es) |
JP (3) | JP2019524147A (es) |
KR (2) | KR102607893B1 (es) |
CN (5) | CN117604021A (es) |
BR (1) | BR112019003114A2 (es) |
CA (1) | CA3033373A1 (es) |
CL (3) | CL2019000421A1 (es) |
CO (1) | CO2019002363A2 (es) |
CR (1) | CR20190130A (es) |
MX (9) | MX2019001956A (es) |
PH (1) | PH12019500341A1 (es) |
UA (1) | UA126801C2 (es) |
UY (1) | UY37369A (es) |
WO (1) | WO2018035354A1 (es) |
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BR112020015486A2 (pt) | 2018-02-15 | 2020-12-08 | Monsanto Technology Llc | Métodos melhorados para a produção de semente de milho híbrido |
BR112020015693A2 (pt) * | 2018-02-15 | 2020-12-08 | Monsanto Technology Llc | Métodos e composições para aumentar o rendimento colheitável através da edição de genes de ga20 oxidase para gerar plantas de estatura curta |
MX2020008561A (es) | 2018-02-15 | 2021-01-08 | Monsanto Technology Llc | Gestión mejorada de maíz a través de sistemas semienanos. |
MX2020008593A (es) * | 2018-02-15 | 2020-11-13 | Monsanto Technology Llc | Composiciones y metodos para mejorar rendimientos de cultivos a traves del apilamiento de rasgos. |
US11275941B2 (en) | 2018-03-08 | 2022-03-15 | Regents Of The University Of Minnesota | Crop models and biometrics |
CN109338001B (zh) * | 2018-11-01 | 2021-05-14 | 浙江省农业科学院 | 一种鉴定水稻半矮秆基因sd1等位基因的分子标记及矮源基因鉴定方法 |
MX2021006525A (es) * | 2018-12-04 | 2021-07-21 | Monsanto Technology Llc | Cosecha retrasada de plantas de maiz de poca altura. |
CN110128518A (zh) * | 2019-05-06 | 2019-08-16 | 中国农业科学院作物科学研究所 | 利用基因编辑技术创制玉米矮化材料的方法 |
MX2021014229A (es) * | 2019-05-29 | 2022-01-06 | Monsanto Technology Llc | Metodos y composiciones para generar alelos dominantes de baja estatura utilizando edicion genomica. |
WO2020243363A1 (en) * | 2019-05-29 | 2020-12-03 | Monsanto Technology Llc | Methods and compositions for generating dominant short stature alleles using genome editing |
US11225669B2 (en) * | 2019-07-18 | 2022-01-18 | Monsanto Technology Llc | Intergenic sequence regions and uses thereof |
WO2021030539A1 (en) * | 2019-08-14 | 2021-02-18 | Monsanto Technology Llc | Compositions and methods for improving crop yields through trait stacking |
UY38833A (es) * | 2019-08-14 | 2021-02-26 | Monsanto Technology Llc | Composiciones y métodos para mejorar los rendimientos de cultivos mediante el apilamiento de rasgos |
JP2021061824A (ja) * | 2019-10-10 | 2021-04-22 | 株式会社カネカ | コムギのゲノム編集方法、及びその利用 |
CN110950942B (zh) * | 2019-12-11 | 2020-12-08 | 广东省农业科学院蔬菜研究所 | 与丝瓜蔓长相关的基因及其应用 |
CN111778265B (zh) * | 2020-07-14 | 2022-06-21 | 吉林省农业科学院 | 玉米赤霉素氧化酶的突变基因、突变体、表达载体和应用 |
CN112062823B (zh) * | 2020-09-22 | 2022-05-17 | 中国农业大学 | Glk7蛋白及其编码基因在植物抗旱中的应用 |
CN112626113B (zh) * | 2020-12-22 | 2022-06-24 | 吉林省农业科学院 | 一种利用基因编辑技术创制玉米矮化材料的方法 |
CN112899292B (zh) * | 2021-02-05 | 2022-10-25 | 浙江农林大学 | 陆地棉株高调控基因GhGA20ox6及其用途 |
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