CA3033373A1 - Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield - Google Patents

Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield Download PDF

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Publication number
CA3033373A1
CA3033373A1 CA3033373A CA3033373A CA3033373A1 CA 3033373 A1 CA3033373 A1 CA 3033373A1 CA 3033373 A CA3033373 A CA 3033373A CA 3033373 A CA3033373 A CA 3033373A CA 3033373 A1 CA3033373 A1 CA 3033373A1
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Prior art keywords
plant
sequence
oxidase
seq
complementary
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CA3033373A
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English (en)
French (fr)
Inventor
Edwards M. Allen
Jayanand BODDU
Charles R. Dietrich
Alexander Goldshmidt
Miya HOWELL
Kevin R. Kosola
Sivalinganna Manjunath
Anil Neelam
Linda RYMARQUIS
Thomas L. SLEWINSKI
Tyamagondlu V. Venkatesh
Huai WANG
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Monsanto Technology LLC
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Monsanto Technology LLC
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684Zea mays [maize]
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    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
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    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • C12N15/8225Leaf-specific, e.g. including petioles, stomata
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    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291Hormone-influenced development
    • C12N15/8297Gibberellins; GA3
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Natural Medicines & Medicinal Plants (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
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  • Enzymes And Modification Thereof (AREA)
CA3033373A 2016-08-17 2017-08-17 Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield Pending CA3033373A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662376298P 2016-08-17 2016-08-17
US62/376,298 2016-08-17
US201762442377P 2017-01-04 2017-01-04
US62/442,377 2017-01-04
US201762502313P 2017-05-05 2017-05-05
US62/502,313 2017-05-05
PCT/US2017/047405 WO2018035354A1 (en) 2016-08-17 2017-08-17 Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield

Publications (1)

Publication Number Publication Date
CA3033373A1 true CA3033373A1 (en) 2018-02-22

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CA3033373A Pending CA3033373A1 (en) 2016-08-17 2017-08-17 Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield

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US (5) US10724047B2 (es)
EP (1) EP3500666A4 (es)
JP (3) JP2019524147A (es)
KR (2) KR20230164771A (es)
CN (4) CN117604024A (es)
BR (1) BR112019003114A2 (es)
CA (1) CA3033373A1 (es)
CL (3) CL2019000421A1 (es)
CO (1) CO2019002363A2 (es)
CR (1) CR20190130A (es)
MX (9) MX2019001956A (es)
PH (1) PH12019500341A1 (es)
UA (1) UA126801C2 (es)
UY (1) UY37369A (es)
WO (1) WO2018035354A1 (es)

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KR20220034872A (ko) * 2019-07-18 2022-03-18 몬산토 테크놀로지 엘엘씨 신규의 유전자간 서열 영역 및 이의 용도
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JP2021061824A (ja) * 2019-10-10 2021-04-22 株式会社カネカ コムギのゲノム編集方法、及びその利用
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CN112062823B (zh) * 2020-09-22 2022-05-17 中国农业大学 Glk7蛋白及其编码基因在植物抗旱中的应用
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