MX2021014229A - Metodos y composiciones para generar alelos dominantes de baja estatura utilizando edicion genomica. - Google Patents

Metodos y composiciones para generar alelos dominantes de baja estatura utilizando edicion genomica.

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Publication number
MX2021014229A
MX2021014229A MX2021014229A MX2021014229A MX2021014229A MX 2021014229 A MX2021014229 A MX 2021014229A MX 2021014229 A MX2021014229 A MX 2021014229A MX 2021014229 A MX2021014229 A MX 2021014229A MX 2021014229 A MX2021014229 A MX 2021014229A
Authority
MX
Mexico
Prior art keywords
methods
compositions
alleles
gibberellin
genome editing
Prior art date
Application number
MX2021014229A
Other languages
English (en)
Inventor
Sivalinganna Manjunath
Thomas L Slewinski
Linda A Rymarquis
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of MX2021014229A publication Critical patent/MX2021014229A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8297—Gibberellins; GA3
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11012—Gibberellin-44 dioxygenase (1.14.11.12)

Abstract

La presente descripción proporciona composiciones y métodos para alterar el contenido de giberelina (GA) en maíz u otras plantas de cereales. Se proporcionan también métodos y composiciones para alterar la expresión de genes relacionados con la biosíntesis de giberelina mediante la edición de subtipos específicos de genes de la oxidasa GA20 para introducir una secuencia o segmento antisentido en el gen. Se proporcionan además células vegetales y plantas modificadas que poseen un alelo dominante que reducen la expresión o actividad de uno o más genes de la oxidasa GA, que comprenden niveles menores de giberelina y mejores características, tales como una menor altura de la planta y una mayor resistencia al encamado, pero sin desviaciones del tipo.
MX2021014229A 2019-05-29 2020-05-28 Metodos y composiciones para generar alelos dominantes de baja estatura utilizando edicion genomica. MX2021014229A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962854142P 2019-05-29 2019-05-29
US201962886726P 2019-08-14 2019-08-14
PCT/US2020/034993 WO2020243361A1 (en) 2019-05-29 2020-05-28 Methods and compositions for generating dominant short stature alleles using genome editing

Publications (1)

Publication Number Publication Date
MX2021014229A true MX2021014229A (es) 2022-01-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021014229A MX2021014229A (es) 2019-05-29 2020-05-28 Metodos y composiciones para generar alelos dominantes de baja estatura utilizando edicion genomica.

Country Status (8)

Country Link
US (1) US20220195445A1 (es)
EP (1) EP3977061A4 (es)
CN (1) CN113557408A (es)
BR (1) BR112021014917A2 (es)
CA (1) CA3131193A1 (es)
MX (1) MX2021014229A (es)
UY (1) UY38724A (es)
WO (1) WO2020243361A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114540529B (zh) * 2022-02-16 2023-07-14 吉林省农业科学院 一种半矮秆增产的玉米基因型、功能性分子标记InDel-K2及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9311147D0 (en) * 1993-05-28 1993-07-14 Long Ashton Research Station Regulation of plant growth
BR112014013853A2 (pt) * 2011-12-09 2020-01-14 Ceres Inc uso de um ácido nucleico isolado, método de obtenção de uma célula vegetal transgênica, método de obtenção de uma planta transgênica, método de produção de uma planta, método de produção de biomassa, método de processamento de biomassa, método de alteração da composição de biomassa, método de modulação de composição de biomassa, método de produção de um produto de forragem
CN117604023A (zh) * 2016-08-17 2024-02-27 孟山都技术公司 通过操纵赤霉素代谢增加可收获产量的用于矮株型植物的方法和组合物
WO2018119225A1 (en) * 2016-12-22 2018-06-28 Monsanto Technology Llc Genome editing-based crop engineering and production of brachytic plants
EP3752621A4 (en) * 2018-02-15 2021-12-01 Monsanto Technology LLC COMPOSITIONS AND METHODS FOR IMPROVING CROP YIELD BY STACKING CHARACTERS

Also Published As

Publication number Publication date
CA3131193A1 (en) 2020-12-03
WO2020243361A9 (en) 2021-11-18
BR112021014917A2 (pt) 2021-09-28
CN113557408A (zh) 2021-10-26
UY38724A (es) 2020-12-31
US20220195445A1 (en) 2022-06-23
EP3977061A1 (en) 2022-04-06
EP3977061A4 (en) 2023-07-26
WO2020243361A1 (en) 2020-12-03

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