CN1352522A - 单子叶植物的超速转化方法 - Google Patents

单子叶植物的超速转化方法 Download PDF

Info

Publication number
CN1352522A
CN1352522A CN99816766A CN99816766A CN1352522A CN 1352522 A CN1352522 A CN 1352522A CN 99816766 A CN99816766 A CN 99816766A CN 99816766 A CN99816766 A CN 99816766A CN 1352522 A CN1352522 A CN 1352522A
Authority
CN
China
Prior art keywords
plant
agrobacterium
seed
gene
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN99816766A
Other languages
English (en)
Other versions
CN1279172C (zh
Inventor
田中宥司
萱野晓明
宇垣正志
盐原文绪
涩谷直人
小野寺治子
小野和子
田切明美
西泽八重子
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Director Of Agricultural Biological Resource Inst Ministry Of Agriculture For
Original Assignee
Director Of Agricultural Biological Resource Inst Ministry Of Agriculture For
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Director Of Agricultural Biological Resource Inst Ministry Of Agriculture For filed Critical Director Of Agricultural Biological Resource Inst Ministry Of Agriculture For
Publication of CN1352522A publication Critical patent/CN1352522A/zh
Application granted granted Critical
Publication of CN1279172C publication Critical patent/CN1279172C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Botany (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Semiconductor Memories (AREA)
  • Electric Clocks (AREA)

Abstract

单子叶植物的土壤杆菌转化方法,它包含用含有所需的重组基因的土壤杆菌感染无伤种子的步骤。

Description

单子叶植物的超速转化方法
技术领域
本发明涉及土壤杆菌介导的单子叶植物的转化方法。
技术背景
“转化技术”是改良植物的手段之一，它将用于改变性状的所需的重组基因导入植物。高效、快速的转化方法对有用植物的分子育种极为重要，尤其对谷物，它是作为主食的重要粮食。
大多数谷物(例如水稻、小麦、大麦及玉米)属于单子叶植物。目前为止已开发出多种转化单子叶植物的转化方法。这些转化方法大体分为直接转化法和间接转化法。
直接转化法包括，如电穿孔法(Shimamofo等，Nature，338：274-276，1989；Rhodes C.A.等，Science，240：204-207，1989)、粒子枪法(ChristouP.等，Bio/Technology 9：957-962，1991)及聚乙二醇(PEG)法(Datta，S.K.等，Bio/Technology 8：736-740，1990)。电穿孔法和粒子枪法通常用于转化单子叶植物，它们是较为有效的导入基因地方法。
间接转化法包括土壤杆菌介导的转化方法(以下叫做土壤杆菌转化法)。土壤杆菌是植物病原菌的一种。土壤杆菌的性质是一旦感染植物，就将自身质粒(例如，Ti质粒或Ri质粒)上存在的T-DNA区域整合到植物中。土壤杆菌转化法利用T-DNA区域整合到植物中作为将基因导入植物的手段。简单地说就是用包含所需的重组基因的土壤杆菌感染植物。感染后，目的基因从土壤杆菌移到植物细胞内，从而整合到植物基因组中。
土壤杆菌转化法对于双子叶植物已建立了有效的方法，到目前为止已建立了大量稳定表达目的基因的转化植物。
与此相对，一般认为土壤杆菌转化法用于单子叶植物很困难。例如，Portrykus等，(Bio/Technology，535-542，1990)报道，土壤杆菌不感染单子叶植物。但是，另一方面，大量尝试了使用土壤杆菌转化单子叶植物，结果发现土壤杆菌转化法适用于单子叶植物的可能性。
例如，Ranineri等取出水稻的胚盘部分，造成损伤，放置到诱导去分化的培养基上，数日后，用土壤杆菌感染其胚盘部分。结果，未得到正常再生个体，但成功诱导了导入外来基因的愈伤组织(RanineriD.M.等，Bio/Technology，8：33-38，1990)。
国际公开WO94/00977号公开了水稻和玉米的土壤杆菌转化法。该方法中有必要应用去分化过程中的或去分化了的培养组织(例如，愈伤组织)作为用土壤杆菌进行转化的植物样品。用土壤杆菌感染前，为了从要被转化的植物样品(例如叶子切片)制备去分化了的培养组织，通常要用3-4周诱导去分化。
国际公开WO95/06722号公开了用土壤杆菌转化水稻和玉米的未成熟胚的方法。但是，取出未成熟胚很麻烦。
因此，若能利用更快速、有效的单子叶植物的土壤杆菌转化法，对包含水稻等谷物的有用单子叶植物的分子育种将是一大贡献。
发明内容
本发明意在解决上述问题。本发明的目的是改良单子叶植物的土壤杆菌转化法。依据本发明的方法可比以往的土壤杆菌转化法更有效地或迅速地制备出转化植物。
本发明涉及单子叶植物的转化方法，它包括用包含所需的重组基因的土壤杆菌感染无伤种子的步骤。本发明的方法中是在无伤状态下感染种子，没必要将要进行转化的植物样品进行去分化处理。
用于被土壤杆菌感染的种子是播种后第4-5天的种子。另外，感染的时间是，种子处于发芽状态。
被转化的单子叶植物优选为水稻科植物，更优选水稻(Oryza sativaL.)。
附图简述
图1是表示生物状态的照片，它表示感染土壤杆菌之前的水稻种子的状态。
图2是表示生物状态的照片，它表示播种后第50天，用本发明的方法而得到的水稻再生个体。
图3(a)是表示生物状态的照片，它比较了播种后第90天用以往的方法得到的转化体与播种后第50天用本发明的方法得到的转化体；图3(b)是表示生物状态的照片，它比较了播种后第50天用以往的方法得到的转化体与播种后第50天用本发明的方法得到的转化体。
本发明的最佳实施方式
以下对本发明进行详细说明。
本发明的方法适用的“植物”为单子叶植物。优选的单子叶植物包括水稻科植物(例如水稻和玉米)。本发明的方法适用的最佳植物是水稻，尤其是Japonica水稻。另外，如无其它说明，“植物”指植物体及由植物体而得到的种子。
(植物表达用载体的构建)
为了将所需的重组基因导入单子叶植物，构建包含所需的重组基因的植物表达用载体。这种植物表达用载体可应用业内人士熟知的基因重组技术进行制备。例如pBI系的载体适用于构建土壤杆菌转化方法中应用的植物表达用载体，但并不限定于此。
“所需的重组基因”是指所希望的导入植物的任何多聚核苷酸。本发明的所需的重组基因并不限定于从天然物分离出的基因，它还包含合成的多聚核苷酸。合成的多聚核苷酸可以这样获得，如用该领域的技术人员所熟知的方法合成或修饰序列已知的基因。本发明的所需的重组基因包括，例如：希望能在转化的植物中表达的、对于植物来讲是内源性的或外源性的任意多聚核苷酸，或者在希望调控植物内某种内源性基因的表达时，包含该目的基因的反义序列的多聚核苷酸。
意图在植物内表达时，所需的重组基因包含可操作方式的自己的启动子(即，与该基因天然可操作性连接到启动子)，或不包含自己的启动子时，或者希望还包含自己启动子之外的启动子时，与任意合适的启动子可操作性连接。所使用的启动子包括组成性启动子，在植物的一部分选择性表达的启动子，及诱导性启动子。
植物表达用载体中还可以在宿主植物细胞内可操作的方式连接各种调控元件。调控元件包括选择性标记基因、植物启动子、终止子及增强子。所使用的植物表达用载体的类型及调控元件的种类要根据转化的目的进行变动，这是业内人士众所周知的事情。
“选择性标记基因”是为了容易筛选转化植物而使用的。可选择性使用赋予潮霉素抗性的潮霉素磷酸转移酶(HPT)基因及赋予新霉素抗性的新霉素磷酸转移酶II(NPTII)这样的耐药基因，但并不限定于此。
“植物启动子”是指可与选择性标记基因可操作性连接、在植物中表达的启动子。这样的启动子的例子有，花椰菜花叶病毒(CaMV)的35S启动子，胭脂碱合成酶基因的启动子，但并不限定于此。
“终止子”指位于基因编码蛋白质区域的下游，DNA转录成mRNA时与转录的终结及polyA序列的添加相关的序列。终止子的例子包括CaMV 35S终止子和胭脂碱合成酶基因的终止子(Tnos)等，但并不限定于此。
“增强子”是为了提高目的基因的表达效率而应用的。作为增强子优选应用包含CaMV 35S启动子上游序列的增强子区域。每一个植物表达用载体可应用多个增强子。
(植物的转化)
用于单子叶植物转化的土壤杆菌可是任意的土壤杆菌属细菌，优选根癌土壤杆菌(Agrobacterium tumefaciens)。用包含所需的重组基因的植物表达用载体(例如通过电穿孔法)转化土壤杆菌。用转化了的土壤杆菌感染种子，从而得到导入了所需的重组基因的植物。导入的重组基因整合到植物的基因组中存在。植物中的基因组不单指核染色体，还包含植物细胞中的各种细胞器(例如线粒体、叶绿体等)中的基因组。
将用于转化的植物的种子去皮后，在无伤状态下预培养。种子“无伤”是指：种子不接受去除种子的胚珠及损伤其胚盘等人为操作的状态。
预培养是将种子撒到含适当浓度植物生长激素(例如2，4-D)的培养基(例如N6D培养基)中，一般保温4-5天，优选5天。预培养在种子组织进入去分化过程以前停止。此时的温度，一般25-35℃，优选27-32℃。预培养结束后，给种子灭菌，然后用水充分洗涤。接着，无菌操作下，用转化的土壤杆菌感染种子。
用土壤杆菌感染种子(共培养)期间，黑暗处将种子一般保温2-5天，优选3天。此时的温度，一般26-38℃，优选28℃。接着，为了去除培养基中的土壤杆菌，用适当的除菌剂(例如羧苄青霉素)处理种子。以选择标记(例如潮霉素抗性等抗药性)为基准筛选转化了的种子。
在适当的除菌条件和筛选条件下培养后，将筛选出的转化种子转移到含适当植物调节物质的再生培养基(例如MS培养基)中，适当的时间内保温。为了让植物体再生，将再生了的转化体移到发根培养基(例如不含植物调节物质的MS培养基)。确定发根后，将转化体入盆。
导入植物的目的重组基因在植物体内发挥预期的目的(例如表达期望的新性状，或者调控某内源性基因的表达)。
可用该领域的技术人员所熟知的方法确认目的重组基因是否导入植物。例如，可用Northern blot分析进行确认。具体而言，从再生植物的叶中提取总RNA，经变性琼脂糖电泳后，点到适当的膜上。该斑点上，导入基因的一部分与互补的标记的RNA探针杂交，由此检测出目的基因的mRNA。或者，希望通过导入目的重组基因来控制植物中内源性基因的表达时，可用上述的Northern blot分析来检测内源性目的基因的表达。当内源性目的基因的表达与非转化的对照植物中该基因的表达被显著抑制时，可确认目的重组基因导入了植物，发挥表达调控的作用。
以往的方法中，在用土壤杆菌感染之前，通常有必要进行3-4周的去分化诱导。与此相对，本发明的方法，没必要进行去分化诱导过程，所以可缩短制备转化的单子叶植物所必要的时间。再者，依照本发明的方法，可缩短以往方法中筛选所需的时间，因此可减低培养变异的影响。
在本发明方法的一个优选实施方案中，制备转化的单子叶植物所需的时间为50天，是以往土壤杆菌转化法(例如参照下述实施例2)所必需时间(约90天)的三分之二以下。另外，依照本发明的方法，应用日本晴的种子时，转化率为10-15％。用Dontokol、Kitaake等其它品种时也能得到同样高的转化率。因此，通过使用本发明的方法，可比以往的转化方法更有效、迅速地制备出转化植物。
(实施例)
列举以下实施例对本发明进行具体说明。本发明并不限定于此。若无其它特殊说明，实施例中所使用的试剂、材料等源自商业渠道。
实施例1：应用本发明的方法进行水稻植物的转化
水稻的代表性品种为日本晴，将日本晴种子去皮后，在无伤状态下，在2.5％次氯酸钠(NaCIO)溶液中灭菌。用水充分洗涤后，将水稻进行以下无菌操作。
1.1预培养
将种子撒到含2，4-D的N6D的培养基(30g/l蔗糖，0.3g/l酪蛋白氨基酸，2.8g/l脯氨酸，2mg/l 2，4-D，4g/l gellite，pH 5.8)上，27-32℃保温5天。这期间种子发芽(图1)。
1.2植物表达用载体
用质粒pIG121Hm(中村等，植物生物技术II，现代化学增刊，pp123-132，(1991))作转化土壤杆菌用的植物表达用载体，该载体是将包含Ricinus的过氧化氢酶第1内含子的GUS基因与潮霉素抗性基因连接的质粒。用pIG121Hm转化土壤杆菌EHA101(Hood等，J.Bacteriol.，168：1291-1301(1986))。EHA101是辅助性质粒的vir区域来自强病原性土壤杆菌A281的细菌。
1.3土壤杆菌感染
将预培养的上述种子浸渍到转化的土壤杆菌的悬浮液中，移植到2N6-AS培养基(，10g/l葡萄糖，0.3g/l酪蛋白氨基酸，2mg/l 2，4-D，10mg/l acetosyringon，4g/l gellite，pH 5.2)中，暗处28℃保温共培养3天。
1.4除菌及筛选
共培养完了后，用含羧苄青霉素的N6D培养基从种子中洗去土壤杆菌。接着在以下条件下筛选转化了的种子。
第一次筛选：将种子放置到含2mg/l补充了羧苄青霉素(500mg/l)和潮霉素(25mg/l)的2，4-D的N6D培养基上，再在27-32℃保温7天。
第二次筛选：将种子放置到含2-4mg/l补充了羧苄青霉素(500mg/l)和潮霉素(25mg/l)的2，4-D的N6D培养基上，再在27-32℃保温7天。
1.5再生
在以下条件下使筛选出的转化种子再生。
第一次再生：将筛选的种子放到再生培养基(补充了羧苄青霉素(500mg/l)和潮霉素(25mg/l)的MS培养基(30g/l蔗糖，30g/l山梨糖醇，2g/l酪蛋白氨基酸，2mg/l激动素，0.002mg/l NAA，4g/l gellite，pH5.8))，27-32℃保温2周。
第二次再生：使用与第一次再生相同的再生培养基，27-32℃再保温2周。
1.6入盆
将再生的转化体移到发根培养基(补充了潮霉素(25mg/l)不含激素的MS培养基)上，确认发根后，入盆(图2)。
实施例2：用以往的方法转化水稻植物
为了与实施例1的方法进行比较，使用日本晴作为转化材料，以往转化水稻植物的方法如下进行。
2.1诱导愈伤组织
将日本晴种子去皮后，灭菌，播种到愈伤组织诱导培养基(含2mg/l2.4-D的N6D培养基)上，在亮处30℃保温。从诱导开始大约4周后，使用来源于胚盘的增殖的愈伤组织进行转化。
2.2转化
如实施例1那样用植物表达用载体pIG121Hm转化土壤杆菌EHA101，用它感染所得愈伤组织，在2N6-AS培养基上，暗处28℃保温共培养3天。
2.3除菌及筛选
用含500mg/l羧苄青霉素的N6D培养基从愈伤组织中洗去土壤杆菌。接着在以下条件下筛选转化了的愈伤组织。
第一次筛选：将愈伤组织放置到含2-4mg/l补充了羧苄青霉素(500mg/l)和潮霉素(25mg/l)的2，4-D的N6D培养基上，再在27-32℃保温2周。
第二次筛选：将愈伤组织放置到含2-4mg/l补充了羧苄青霉素(500mg/l)和潮霉素(25mg/l)的2，4-D的N6D培养基上，再在27-32℃保温2周。
2.4再生、发根及入盆
在与实施例1同样的条件下使筛选出的转化种子再生、入盆。
2.5结果
用以往的方法进行转化的实施例与用本发明的方法进行转化的实施例进行比较的结果如图3所示。播种后到入盆所必需的日期是，以往的方法中约90天，而本发明的方法中约50天(图3(a))。在播种后第50天的时间点进行比较，本发明方法中的转化体已达到可入盆的状态，而以往的方法中，转化体还处于再生过程(图3(b))。总之，本发明的方法将转化所需的时间缩短到以往的约三分之二以下。
产业实用性
本发明提供改良的、土壤杆菌介导的单子叶植物的转化方法。本发明的方法中，用包含所需的重组基因的土壤杆菌感染打算用于转化的植物的无伤的种子。通过使用本发明，可更有效且迅速地制备出转化植物。

Claims (5)

1转化单子叶植物的方法，它包含用含有所需重组基因的土壤杆菌感染无伤种子的步骤。
2权利要求1的方法，其中的种子是播种后第4-5天的种子。
3权利要求2的方法，其中的种子是发芽的种子。
4权利要求3的方法，其中单子叶植物是水稻科植物。
5权利要求4的方法，其中水稻科植物是水稻。
CNB998167665A 1999-07-22 1999-07-22 转化水稻植物的方法 Expired - Fee Related CN1279172C (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP1999/003920 WO2001006844A1 (fr) 1999-07-22 1999-07-22 Procede de transformation ultrarapide de monocotyledon

Publications (2)

Publication Number Publication Date
CN1352522A true CN1352522A (zh) 2002-06-05
CN1279172C CN1279172C (zh) 2006-10-11

Family

ID=14236281

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB998167665A Expired - Fee Related CN1279172C (zh) 1999-07-22 1999-07-22 转化水稻植物的方法

Country Status (9)

Country Link
EP (1) EP1198985B1 (zh)
KR (1) KR100455617B1 (zh)
CN (1) CN1279172C (zh)
AT (1) ATE480140T1 (zh)
AU (1) AU775233B2 (zh)
CA (1) CA2366104C (zh)
DE (1) DE69942750D1 (zh)
HK (1) HK1045237B (zh)
WO (1) WO2001006844A1 (zh)

Families Citing this family (161)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100402513B1 (ko) * 2001-02-16 2003-10-22 주식회사 싸이젠하베스트 유전자 조작을 통한 식물체의 효과적인 형질전환 방법
ATE509111T1 (de) 2002-03-20 2011-05-15 Simplot Co J R Verfeinerte pflanzentransformation
US7847156B2 (en) 2003-10-20 2010-12-07 Cropdesign N.V. Plants having improved growth characteristics and methods for making the same
KR20040042619A (ko) * 2002-11-15 2004-05-20 최용의 아그로박테리움 튜머파시언스를 이용한 오갈피 형질전환 방법
CN1732266B (zh) 2002-12-24 2011-11-16 作物培植股份有限公司 具有改变的生长特性的植物及其生产方法
EP1613146B1 (en) 2003-04-14 2013-06-19 CropDesign N.V. Plants having modified growth characteristics and method for making the same
ATE448314T1 (de) 2003-09-05 2009-11-15 Cropdesign Nv Pflanzen mit verbesserten wachstumseigenschaften sowie verfahren zu deren herstellung
CN1914323B (zh) 2003-12-17 2012-10-10 作物培植股份有限公司 具有改良的生长特性的植物及其制备方法
CN1946848A (zh) 2004-03-01 2007-04-11 克罗普迪塞恩股份有限公司 产量增加的植物及其制备方法
EP1580275B1 (en) 2004-03-22 2009-09-02 CropDesign N.V. Plants having increased seed yield and method for making the same
JPWO2005092082A1 (ja) * 2004-03-25 2008-02-07 独立行政法人農業生物資源研究所 単子葉植物の種子の形質転換法
RU2409938C2 (ru) 2004-04-02 2011-01-27 КРОПДИЗАЙН Н.Фи. Способ повышения выхода семян растения, способ производства трансгенного растения, имеющего повышенную урожайность семян, генная конструкция для экспрессии в растении и трансгенное растение
US7825293B2 (en) 2004-05-28 2010-11-02 Cropdesign N.V. Plants having improved growth characteristics and a method for making the same
WO2006005751A1 (en) 2004-07-12 2006-01-19 Cropdesign N.V. Plants having improved growth characteristics and method for making the same
EP2166105A3 (en) 2004-08-16 2010-04-14 CropDesign N.V. Plants having improved growth characteristics and method for making the same
CN101022719B (zh) 2004-09-16 2010-06-09 克罗普迪塞恩股份有限公司 评价植物根的方法和装置
ES2358913T3 (es) 2004-10-29 2011-05-16 Cropdesign N.V. Plantas que tienen características de crecimiento mejoradas y método para obtener la mismas.
AR051866A1 (es) 2004-12-24 2007-02-14 Cropdesign Nv Plantas con aumento del rendimiento y metodo de prepracion
BRPI0607211A2 (pt) 2005-01-27 2009-12-22 Cropdesign Nv métodos para incrementar rendimento de planta relativamente a plantas correspondentes de tipo selvagem, para incrementar rendimento, particularmente rendimento de sementes, relativamente àquele de plantas correspondentes de tipo selvagem e para a produção de uma planta transgênica, planta, parte de planta ou célula de planta, construção, planta transgênica ou parte da mesma, partes colhìveis, produtos, e, uso de um gene/ácido nucleico de syt ou variante do mesmo, ou uso de um polipeptìdeo de syt ou homólogo do mesmo, ou uso de uma construção
US8029482B2 (en) 2005-03-04 2011-10-04 C. R. Bard, Inc. Systems and methods for radiographically identifying an access port
CN101258246B (zh) 2005-07-06 2012-09-05 克罗普迪塞恩股份有限公司 Ste20样基因表达对植物产率的提高
US8071840B2 (en) 2005-09-15 2011-12-06 Cropdesign N.V. Plants having increase yield and method for making the same
WO2007051866A2 (en) 2005-11-07 2007-05-10 Cropdesign N.V. Plants having improved growth characteristics and a method for making the same
US8853492B2 (en) 2005-11-07 2014-10-07 Cropdesign N.V. Plants having improved growth characteristics and a method for making the same
AR056790A1 (es) 2005-11-08 2007-10-24 Cropdesign Nv Plantas con caracteristicas de crecimiento aumentado y un metodo para obtenerlas
AU2006320596B2 (en) 2005-12-01 2013-02-07 Cropdesign N.V. Plants having improved growth characteristics and methods for making the same
ES2390919T3 (es) 2006-03-31 2012-11-19 Basf Plant Science Gmbh Plantas que tienen rasgos relacionados con el rendimiento mejorados y un método para elaborar las mismas
EP2441839A1 (en) 2006-05-30 2012-04-18 CropDesign N.V. Plants with reduced expression of REVOLUTA (REV) having enhanced yield-related traits and a method for making the same
CN101460611B (zh) 2006-06-08 2013-04-24 巴斯福植物科学有限公司 具有改良生长特性的植物及其制备方法
CA2654100A1 (en) 2006-06-15 2007-12-21 Cropdesign N.V. Use of nac transcription factor for enchancing yield-related traits in plants
EP2599870A3 (en) 2006-06-15 2013-09-11 CropDesign N.V. Plants having enhanced yield-related traits and a method for making the same
ES2451669T3 (es) 2006-08-02 2014-03-28 Cropdesign N.V. Plantas que tienen características mejoradas y un procedimiento de fabricación de las mismas
EP2199395A1 (en) 2006-08-02 2010-06-23 CropDesign N.V. Plants transformed with SYT-polypeptide having increased yield under abiotic stress and a method for making the same
WO2008059048A1 (en) 2006-11-16 2008-05-22 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same using consensus sequences from the yabby protein family
US8350119B2 (en) 2006-11-24 2013-01-08 Cropdesign N.V. Transgenic plants comprising as transgene A class I TCP or Clavata 1 (CLV1) or CAH3 polypeptide having increased seed yield and a method for making the same
WO2008071767A1 (en) 2006-12-15 2008-06-19 Cropdesign N.V. Plants having enhanced seed yield-related traits and a method for making the same
WO2008074891A2 (en) 2006-12-21 2008-06-26 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for method for making the same
AR065100A1 (es) 2007-01-30 2009-05-13 Cropdesign Nv Plantas con rasgos relacionados con el rendimiento mejorado y un metodo para producirlas
ES2485384T3 (es) 2007-01-31 2014-08-13 Basf Plant Science Gmbh Plantas que tienen rasgos potenciados relacionados con el rendimiento y/o mayor resistencia al estrés abiótico y un procedimiento para fabricar las mismas
DE112008000521T5 (de) 2007-02-28 2010-03-04 Cropdesign N.V. Pflanzen mit verbesserten Ertragsmerkmalen und Verfahren zu ihrer Herstellung
BRPI0810703A2 (pt) 2007-05-03 2012-12-25 Basf Plant Science Gmbh mÉtodo para intensificar traÇos relacionados com rendimento em plantas com relaÇço Ás plantas de controle, molÉcula de Ácido nucleico isolada, polipeptÍdeo isolado, mÉtodo para a produÇço de uma planta transgÊnica, planta ou parte da mesma, construÇço, e, uso de um polipeptÍdeo ou de um Ácido nucleico
BRPI0811185A2 (pt) 2007-05-23 2014-10-07 Cropdesign Nv Método para intensificação de características relacionadas a rendimento em plantas com relação às plantas de controle, planta, parte de planta ou célula de planta, construto, uso de um construto, método para a produção de uma planta transgênica tendo características relacionadas a rendimento intensificadas com relação às plantas de controle, planta transgênica, partes colhíveis de uma planta, produtos derivados de uma planta, e, uso de uma seqüência de ácido nucleico
WO2009003977A2 (en) 2007-06-29 2009-01-08 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
WO2009013263A2 (en) 2007-07-20 2009-01-29 Basf Plant Science Gmbh Plants having increased yield-related traits and a method for making the same
AR067748A1 (es) 2007-07-31 2009-10-21 Basf Plant Science Gmbh Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas
AR067766A1 (es) 2007-07-31 2009-10-21 Basf Plant Science Gmbh Metodo para modular la expresion de una planta de fosfoenolpiruvato carboxilasa (pepc), proteina de la caja u de clase iii y proteina de pqqc.
US9617557B2 (en) 2007-09-14 2017-04-11 Basf Plant Science Gmbh Plants having increased yield-related traits by expressing a growth-regulating factor (GRF) polypeptide and method for making the same
AU2008300478B2 (en) 2007-09-21 2014-11-06 Basf Plant Science Gmbh Plants having increased yield-related traits and a method for making the same
DE112008002848T5 (de) 2007-10-29 2010-11-25 Basf Plant Science Gmbh Pflanzen mit verbesserten Ertragsmerkmalen und Verfahren zu ihrer Herstellung
MX2010004699A (es) 2007-11-22 2010-05-14 Cropdesign Nv Plantas que tienen rasgos incrementados relacionados con el rendimiento y un metodo para producirlas.
EP2581385A1 (en) 2007-11-26 2013-04-17 BASF Plant Science GmbH Plants having enhanced yield-related traits and a method for making the same
WO2009080802A2 (en) 2007-12-20 2009-07-02 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
US20110209241A1 (en) 2008-01-25 2011-08-25 Basf Plant Science Gmbh Plants Having Enhanced Yield-Related Traits and a Method for Making the Same
RU2522480C2 (ru) 2008-01-31 2014-07-20 Нэшнл Инститьют Фо Байолоджикэл Сайенсес Растения с измененным ростом и/или развитием и способ их создания
AU2009237642A1 (en) 2008-04-16 2009-10-22 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
US20110061134A1 (en) 2008-05-05 2011-03-10 Basf Plant Science Gmbh Plants Having Enhanced Yield-Related Traits and a Method for Making the Same
US20110099669A1 (en) 2008-06-20 2011-04-28 Basf Plant Science Gmbh Plants having enhanced yield-related traits and a method for making the same
CN103789343A (zh) 2008-06-26 2014-05-14 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物及其制备方法
DE112009001560T5 (de) 2008-07-04 2012-01-26 Basf Plant Science Gmbh Planzen mit gesteigerten ertragsbezogenen Eigenschaften und Verfahren zur Herstellung derselben
EP2657341A3 (en) 2008-07-17 2014-01-22 BASF Plant Science GmbH Plants having enhanced yield-related traits and a method for making the same
MX2011000778A (es) 2008-07-31 2011-03-15 Basf Plant Science Gmbh Plantas con caracteristicas modificadas de crecimiento y un metodo para obtenerlas.
MX2011001475A (es) 2008-08-20 2011-03-29 Basf Plant Science Gmbh Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas.
EP3081077B1 (en) 2008-09-16 2019-06-19 BASF Plant Science GmbH Method for improved plant breeding
EA201100515A1 (ru) 2008-09-24 2012-02-28 Басф Плант Сайенс Гмбх Растения с улучшенными характеристиками урожайности и способ их получения
AU2009315732A1 (en) 2008-11-12 2010-05-20 Basf Plant Science Gmbh Plants having enhanced abiotic stress tolerance and/or enhanced yield-related traits and a method for making the same
JP5458348B2 (ja) * 2008-12-02 2014-04-02 国立大学法人九州大学 コエンザイムｑ１０強化糖質突然変異植物体およびその製造方法
DE112009003515T5 (de) 2008-12-03 2012-05-24 Basf Plant Science Gmbh Pflanzen mit gesteigerter abiotischer Stresstoleranz und/oder gesteigerten ertragsbezogenen Eigenschaften und Verfahren zur Herstellung derselben
DE112009003749T5 (de) 2008-12-17 2012-11-15 Basf Plant Science Gmbh Pflanzen mit gesteigerten ertragsbezogenen Eigenschaften und/oder gesteigerter abiotischerStresstoleranz und Verfahren zur Herstellung derselben
CN104232679A (zh) 2009-01-28 2014-12-24 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物及其制备方法
BRPI1008725A2 (pt) 2009-02-25 2016-03-08 Basf Plant Science Co Gmbh métodos para intensificar e melhorar características relacionadas ao rendimento em plantas com relação às plantas de controle, e para a produção de uma planta transgênica, construto, usos do construto, de um ácido nucleico, e de uma sequência de ácido nucleic, planta, parte da planta ou célula da planta, planta transgênica, partes colhíveis, produtos, molécula de ácido nucleico isolada, e, polipeptídeo isolado
US20120102599A1 (en) 2009-04-29 2012-04-26 Crop Functional Genomics Center Plants Having Enhanced Yield-Related Traits and a Method for Making the Same
CN102459613A (zh) 2009-04-29 2012-05-16 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物及其制备方法
WO2010127969A1 (en) 2009-05-06 2010-11-11 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and/or enhanced abiotic stress tolerance and a method for making the same
WO2011006717A2 (en) 2009-06-19 2011-01-20 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
EA201200284A1 (ru) 2009-08-19 2013-03-29 Басф Плант Сайенс Компани Гмбх Растения, имеющие улучшенные характеристики, связанные с урожаем, и способ создания таких
DE112010003793T5 (de) 2009-09-25 2012-11-22 Basf Plant Science Company Gmbh Pflanzen mit gesteigerten Ertragsmerkmalen und Verfahren zu deren Herstellung
MX2012003451A (es) 2009-09-25 2012-05-22 Basf Plant Science Co Gmbh Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para prepararlas.
CA2777802A1 (en) 2009-10-22 2011-04-28 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
DE112010004383T5 (de) 2009-11-13 2012-08-23 Basf Plant Science Company Gmbh Pflanzen mit gesteigerten Ertragsmerkmalen und Verfahren zu deren Herstellung
WO2011104155A2 (en) 2010-02-24 2011-09-01 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
MX2012009524A (es) 2010-02-24 2012-09-12 Basf Plant Science Co Gmbh Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas.
EP2361985A1 (en) 2010-02-26 2011-08-31 BASF Plant Science Company GmbH Plants having enhanced yield-related traits and a method for making the same
EP2361927A1 (en) 2010-02-26 2011-08-31 BASF Plant Science Company GmbH Plants having enhanced yield-related traits and a method for making the same
MX2012010221A (es) 2010-03-18 2013-02-26 Basf Plant Science Co Gmbh Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas.
MX2012010600A (es) 2010-03-18 2012-10-05 Basf Plant Science Co Gmbh Plantas que tienen mejores rasgos relacionados con el redimiento y un metodo para producirlas.
US20130019347A1 (en) 2010-03-19 2013-01-17 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
US20130025002A1 (en) 2010-03-19 2013-01-24 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
EP2371845A1 (en) 2010-03-22 2011-10-05 BASF Plant Science Company GmbH Plants having enhanced yield-related traits and a method for making the same
JP2013529074A (ja) 2010-05-04 2013-07-18 ビーエーエスエフ ソシエタス・ヨーロピア 除草剤に対する耐性が増加した植物
CN103068992A (zh) 2010-06-24 2013-04-24 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物和用于制备该植物的方法
KR101429473B1 (ko) 2010-06-25 2014-08-22 바스프 플랜트 사이언스 컴퍼니 게엠베하 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법
AU2011277925A1 (en) 2010-07-16 2013-02-21 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and method for making the same
EP2835427A3 (en) 2010-08-24 2015-05-20 BASF Plant Science Company GmbH Plants having enhanced yield-related traits and a method for making the same
EP2426204A1 (en) 2010-09-02 2012-03-07 Ludwig-Maximilians-Universität München Spontaneous nodule organogenesis in plants
WO2012028673A1 (en) 2010-09-02 2012-03-08 Ludwig-Maximilians-Universitaet Muenchen Spontaneous organogenesis in plants
WO2012063200A1 (en) 2010-11-10 2012-05-18 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and method for making the same
CN103261424B (zh) 2010-12-16 2017-12-12 巴斯夫农业公司 对除草剂具有增强的耐受性的植物
WO2012098517A1 (en) 2011-01-20 2012-07-26 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and method for making the same
EP2681325A4 (en) 2011-02-28 2014-10-22 Basf Plant Science Co Gmbh PLANTS WITH IMPROVED EFFICIENCY AND MANUFACTURING METHOD THEREFOR
US20130340120A1 (en) 2011-02-28 2013-12-19 Basf Plant Science Company Gmbh Plants Having Enhanced Yield-Related Traits and Producing Methods Thereof
AU2012222946A1 (en) 2011-03-01 2013-09-19 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and producing methods thereof
ES2679995T3 (es) 2011-03-11 2018-09-03 Vib Vzw Moléculas y métodos para la inhibición y detección de proteínas
GB201106845D0 (en) 2011-04-26 2011-06-01 Vib Vzw Methods and means to produce abiotic stress tolerant plants
US9422573B2 (en) 2011-05-11 2016-08-23 Vib Vzw Methods and means for generating microbial disease resistant plants
CA2841782A1 (en) 2011-07-15 2013-01-24 Syngenta Participations Ag Methods of increasing yield and stress tolerance in a plant
WO2013012643A1 (en) 2011-07-15 2013-01-24 Syngenta Participations Ag Polynucleotides encoding trehalose-6-phosphate phosphatase and methods of use thereof
GB201116129D0 (en) 2011-09-19 2011-11-02 Vib Vzw Methods and means to produce abiotic stress tolerant plants
KR101317891B1 (ko) * 2011-11-04 2013-10-11 대한민국(농촌진흥청장) 발아초기 종자 이용 단기 형질전환 벼 식물체의 제조 방법 및 그에 따른 식물체
WO2013092275A2 (en) 2011-12-23 2013-06-27 Basf Plant Science Company Gmbh Genes to enhance the defense against pathogens in plants
AR091489A1 (es) 2012-06-19 2015-02-11 Basf Se Plantas que tienen una mayor tolerancia a herbicidas inhibidores de la protoporfirinogeno oxidasa (ppo)
EP2677035A1 (en) 2012-06-22 2013-12-25 BASF Plant Science Company GmbH Plants having enhanced yield-related traits and a method for making the same
US10006041B2 (en) 2012-08-16 2018-06-26 Vib Vzw Means and methods for altering the lignin pathway in plants
WO2014147249A1 (en) 2013-03-21 2014-09-25 Vib Vzw Means and methods for the reduction of photorespiration in crops
TR201819577T4 (tr) 2013-04-29 2019-01-21 Agrosavfe N V Sfingolipitleri bağlayan antikorlar içeren agrokimyasal bileşimler.
EP2992102A4 (en) 2013-04-30 2016-12-28 Basf Se PLANTS WITH INCREASED TOLERANCE AGAINST HERBICIDES
GB201309866D0 (en) 2013-06-03 2013-07-17 Vib Vzw Means and methods for yield performance in plants
WO2014198885A1 (en) 2013-06-14 2014-12-18 Bayer Cropscience Nv Plant fibers with improved dyeing properties
EP2816115A1 (en) 2013-06-17 2014-12-24 BASF Plant Science Company GmbH Plants having one or more enhanced yield-related traits and a method for making the same
EP2896698A1 (en) 2014-01-17 2015-07-22 BASF Plant Science Company GmbH Plants having one or more enhanced yield-related traits and a method for making the same
WO2015150465A2 (en) 2014-04-03 2015-10-08 Basf Se Plants having increased tolerance to herbicides
CA2954201A1 (en) 2014-07-08 2016-01-14 Vib Vzw Means and methods to increase plant yield
EP3201327B1 (en) 2014-10-03 2018-10-03 VIB vzw Functional mutant alleles of ein5 for yield increase
JP7089877B2 (ja) 2014-11-05 2022-06-23 バイオタリス・エン・フェー 重鎖抗体の可変ドメインをコードするポリヌクレオチドを含むトランスジェニック植物
EP3247200A4 (en) 2015-01-21 2018-06-13 Basf Se Plants having increased tolerance to herbicides
US20180312864A1 (en) 2015-10-22 2018-11-01 Basf Se Plants having increased tolerance to herbicides
CN109154003A (zh) 2016-05-20 2019-01-04 巴斯夫农业公司 用于靶向多肽的双转运肽
US10897903B2 (en) 2016-05-24 2021-01-26 Basf Se Method for controlling PPO resistant weeds
CA3030803A1 (en) 2016-07-15 2018-01-18 Basf Se Plants having increased tolerance to herbicides
BR112019001483A2 (pt) 2016-07-27 2019-09-10 Basf Agro Bv método para controlar a vegetação indesejada em um local de cultivo de plantas
CA3030354A1 (en) 2016-07-29 2018-02-01 Basf Se Method for controlling ppo resistant weeds
WO2018019845A1 (en) 2016-07-29 2018-02-01 Basf Se Method for controlling ppo resistant weeds
US20210352900A1 (en) 2016-08-05 2021-11-18 Basf Se Method for Controlling PPO Resistant Weeds
EP3278667A1 (en) 2016-08-05 2018-02-07 Basf Se Method for controlling ppo-inhibitor resistant weeds
US20210368793A1 (en) 2016-08-05 2021-12-02 Basf Se Method for Controlling PPO Resistant Weeds
BR112019001862B1 (pt) 2016-08-09 2022-10-18 Basf Se Métodos para controlar o crescimento de ervas daninhas resistentes a ppo, e uso do composto de fórmula (i).1
EP3281525A1 (en) 2016-08-09 2018-02-14 Basf Se Method for controlling ppo resistant weeds
EP3281523A1 (en) 2016-08-09 2018-02-14 Basf Se Method for controlling ppo resistant weeds
WO2018029031A1 (en) 2016-08-09 2018-02-15 Basf Se Method for controlling ppo resistant weeds
CA3032223A1 (en) 2016-08-09 2018-02-15 Basf Se Method for controlling ppo resistant weeds
EP3281524A1 (en) 2016-08-09 2018-02-14 Basf Se Method for controlling ppo resistant weeds
EP3510158A1 (en) 2016-09-06 2019-07-17 Vib Vzw Means and methods to increase coumarin production
CA3044728A1 (en) 2016-12-20 2018-06-28 BASF Agro B.V. Plants having increased tolerance to herbicides
AU2018376915A1 (en) 2017-11-29 2020-05-21 Basf Se Plants having increased tolerance to herbicides
BR112020013929A2 (pt) 2018-01-17 2020-12-01 Basf Se plantas ou partes da planta, semente, células vegetais, produto vegetal, progênie ou planta descendente, métodos para controlar ervas daninhas, para produzir uma planta e para produzir uma planta descendente, molécula de ácido nucleico, cassete de expressão, vetor, polipeptídeo, método para produzir um produto vegetal e produto vegetal
GB201809273D0 (en) 2018-06-06 2018-07-25 Vib Vzw Novel mutant plant cinnamoyl-coa reductase proteins
GB201813011D0 (en) 2018-08-10 2018-09-26 Vib Vzw Means and methods for drought tolerance in crops
WO2020049155A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
US20210317467A1 (en) 2018-09-07 2021-10-14 Basf Plant Science Copmany Gmbh Improved method for the production of high levels of pufa in plants
CA3110656A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
GB201817464D0 (en) 2018-10-26 2018-12-12 Vib Vzw Means and methods to increase organ size in plants
GB201820109D0 (en) 2018-12-11 2019-01-23 Vib Vzw Plants with a lignin trait and udp-glycosyltransferase mutation
CA3124395A1 (en) 2018-12-20 2020-06-25 Basf Plant Science Company Gmbh Native delivery of biomolecules into plant cells using ionic complexes with cell-penetrating peptides
EP4125379A1 (en) 2020-03-31 2023-02-08 Biotalys NV Anti-fungal polypeptides
WO2022152660A1 (en) 2021-01-12 2022-07-21 Vib Vzw Means and methods for producing drought tolerant cereals
CN117897378A (zh) 2021-09-03 2024-04-16 巴斯夫农业种子解决方案美国有限责任公司 对除草剂具有增加的耐受性的植物
WO2023057601A1 (en) 2021-10-06 2023-04-13 Biotalys NV Anti-fungal polypeptides
WO2023131616A1 (en) 2022-01-05 2023-07-13 Vib Vzw Means and methods to increase abiotic stress tolerance in plants
WO2023131637A1 (en) 2022-01-06 2023-07-13 Vib Vzw Improved silage grasses
WO2023144199A1 (en) 2022-01-26 2023-08-03 Vib Vzw Plants having reduced levels of bitter taste metabolites
WO2024047057A1 (en) 2022-09-01 2024-03-07 Vib Vzw Means and methods to produce triterpene saponins in eukaryotic cells
WO2024126113A1 (en) 2022-12-12 2024-06-20 BASF Agricultural Solutions Seed US LLC Plants having increased tolerance to herbicides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591616A (en) * 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons

Also Published As

Publication number Publication date
EP1198985B1 (en) 2010-09-08
WO2001006844A1 (fr) 2001-02-01
KR20010109314A (ko) 2001-12-08
AU775233B2 (en) 2004-07-22
CA2366104A1 (en) 2001-02-01
CN1279172C (zh) 2006-10-11
AU4799099A (en) 2001-02-13
EP1198985A1 (en) 2002-04-24
KR100455617B1 (ko) 2004-11-06
HK1045237A1 (en) 2002-11-22
ATE480140T1 (de) 2010-09-15
CA2366104C (en) 2010-07-06
HK1045237B (zh) 2011-03-11
EP1198985A4 (en) 2004-09-29
DE69942750D1 (de) 2010-10-21

Similar Documents

Publication Publication Date Title
CN1279172C (zh) 转化水稻植物的方法
Pino et al. The Rg1 allele as a valuable tool for genetic transformation of the tomato'Micro-Tom'model system
CN87106120A (zh) 种子特异性转录调节
Hankoua et al. Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos
Bakshi et al. Seedling preconditioning in thidiazuron enhances axillary shoot proliferation and recovery of transgenic cowpea plants
Chen et al. Agrobacterium tumefaciens-mediated transformation system for the important medicinal plant Dendrobium catenatum Lindl
CN113512547B (zh) 一种橡胶树泛素基因启动子proHbUBI1及其克隆与应用
Sarria et al. Agrobacterium-mediated transformation of Stylosanthes guianensis and production of transgenic plants
JP3141084B2 (ja) 単子葉植物の超迅速形質転換法
US20040237133A1 (en) Method for transformation of mono-and di-cotyledonous plants using meristematic tissue and nodal callus from dicotyledonous plants
Tang et al. Slash pine genetic transformation through embryo cocultivation with A. tumefaciens and transgenic plant regeneration
EP0996329A1 (en) Method of genetically transforming banana plants
Akbulut et al. Analysis and optimization of DNA delivery into chickpea (Cicer arietinum L.) seedlings by Agrobacterium tumefacience
Thirukkumaran et al. Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida
CN114774464A (zh) 一种农杆菌介导甘蔗愈伤组织高效遗传转化方法
Duan et al. Co-culturing on dry filter paper significantly increased the efficiency of Agrobacterium-mediated transformations of maize immature embryos
CN1137995C (zh) 一种建立植物基因标签系统的方法
US8519227B2 (en) Ultra-fast transformation technique for monocotyledons
Raman et al. Rapid and efficient Agrobacterium mediated transformation of early scutellum derived calli of indica rice
KR101198648B1 (ko) 직접 신초 유도를 통한 오이 계통의 형질전환 방법 및 상기방법에 의해 제조된 오이 계통 형질전환체
Shi et al. Improved production of transgenic Dioscorea zingiberensis (Dioscoreaceae) by Agrobacterium tumefaciens-mediated transformation
CN101260405A (zh) 一种植物生长素调控的启动子及其应用
US20130347144A1 (en) Promoters and methods for transforming tubers and transformed tubers
CN118290557A (zh) 水稻分蘖数量调控的Tn1a蛋白及其相关生物材料与应用
CA2830548A1 (en) Methods for transforming tarwi and for producing molecular farming products in transgenic tarwi seed

Legal Events

Date Code Title Description
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C06 Publication
PB01 Publication
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20061011

Termination date: 20150722

EXPY Termination of patent right or utility model