CN1249784A - 一种体外构建dna文库的方法 - Google Patents

一种体外构建dna文库的方法 Download PDF

Info

Publication number
CN1249784A
CN1249784A CN98802958A CN98802958A CN1249784A CN 1249784 A CN1249784 A CN 1249784A CN 98802958 A CN98802958 A CN 98802958A CN 98802958 A CN98802958 A CN 98802958A CN 1249784 A CN1249784 A CN 1249784A
Authority
CN
China
Prior art keywords
template
polymerase
dna
primers
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN98802958A
Other languages
English (en)
Chinese (zh)
Inventor
杰斯珀·文德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of CN1249784A publication Critical patent/CN1249784A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN98802958A 1997-03-18 1998-03-18 一种体外构建dna文库的方法 Pending CN1249784A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK30797 1997-03-18
DK0307/1997 1997-03-18
DK0434/1997 1997-04-17
DK43497 1997-04-17
DK0625/1997 1997-05-30
DK62597 1997-05-30

Publications (1)

Publication Number Publication Date
CN1249784A true CN1249784A (zh) 2000-04-05

Family

ID=27220576

Family Applications (1)

Application Number Title Priority Date Filing Date
CN98802958A Pending CN1249784A (zh) 1997-03-18 1998-03-18 一种体外构建dna文库的方法

Country Status (14)

Country Link
EP (1) EP0973940B1 (enExample)
JP (1) JP2001515356A (enExample)
KR (1) KR20000076363A (enExample)
CN (1) CN1249784A (enExample)
AT (1) ATE399880T1 (enExample)
AU (1) AU741834B2 (enExample)
BR (1) BR9808368A (enExample)
CA (1) CA2284253A1 (enExample)
DE (1) DE69839668D1 (enExample)
DK (1) DK0973940T3 (enExample)
IL (1) IL131966A0 (enExample)
NZ (1) NZ337900A (enExample)
PL (1) PL335860A1 (enExample)
WO (1) WO1998041653A1 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103539744A (zh) * 2013-10-17 2014-01-29 北京颖泰嘉和生物科技有限公司 一种制备3-(3,5-二氯苯基)-2,4-咪唑烷二酮的方法
WO2016134521A1 (en) * 2015-02-27 2016-09-01 The University Of Hong Kong Dna display and methods thereof
CN108588050A (zh) * 2018-05-14 2018-09-28 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
CN114197061A (zh) * 2022-02-17 2022-03-18 北京贝瑞和康生物技术有限公司 用于构建检测染色体拷贝数变异的测序文库的方法和试剂盒
CN117157148A (zh) * 2021-02-08 2023-12-01 胡桃夹子治疗公司 制备合成模板的方法

Families Citing this family (126)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
KR20010071613A (ko) * 1998-06-29 2001-07-28 추후기재 매우 다양한 라이브러리의 생산방법
EP1104459A1 (en) 1998-08-12 2001-06-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
EP1119616A2 (en) 1998-10-07 2001-08-01 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000024883A1 (en) 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
AU1619400A (en) 1998-11-10 2000-05-29 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plantphenotypes
ES2328429T3 (es) * 1998-11-27 2009-11-12 Novozymes A/S Variantes de enzimas lipoliticas.
US7312062B2 (en) 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
AU3210100A (en) 1999-01-19 2000-08-01 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
KR20010102069A (ko) 1999-02-11 2001-11-15 추후제출 고효율 질량 분석
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
WO2000073433A1 (en) * 1999-05-31 2000-12-07 Novozymes A/S Screening method for peptides
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
WO2001051663A2 (en) 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
WO2002000897A2 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel chimeric promoters
CA2411828A1 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US6783941B2 (en) 2000-12-06 2004-08-31 Novozymes A/S Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
AU2002220529A1 (en) * 2000-12-06 2002-06-18 Novozymes A/S Method for producing a polynucleotide library
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
WO2002083868A2 (en) 2001-04-16 2002-10-24 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
WO2003008563A2 (en) 2001-07-20 2003-01-30 California Institute Of Technology Improved cytochrome p450 oxygenases
EP1493027B1 (en) 2002-03-01 2014-10-15 Codexis Mayflower Holdings, LLC Methods, systems, and software for identifying functional bio-molecules
JP4851687B2 (ja) 2002-03-09 2012-01-11 マキシジェン， インコーポレイテッド 定向進化のための交叉点の最適化
CN102174546A (zh) 2002-04-19 2011-09-07 维莱尼姆公司 磷脂酶，编码磷脂酶的核酸以及制备和应用磷脂酶的方法
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7262012B2 (en) 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
EP2275536A1 (en) 2002-08-06 2011-01-19 Verdia, Inc. AP1 amine oxidase variants
WO2004016811A2 (en) * 2002-08-19 2004-02-26 Danmarks Tekniske Universitet (Dtu) Methods and kit for genes shuffling using tagged primers
CN102618564B (zh) 2003-03-06 2014-10-29 维莱尼姆公司 淀粉酶、编码它们的核酸及其制备和应用方法
DK2853593T3 (en) 2003-03-07 2018-01-08 Dsm Ip Assets Bv Hydrolases, nucleic acids encoding them, and processes for their preparation and use
PL1613733T3 (pl) 2003-04-04 2015-11-30 Basf Enzymes Llc Liazy pektynianowe, kodujące je kwasy nukleinowe oraz sposoby ich wytwarzania i zastosowania
EP1620557A2 (en) 2003-04-29 2006-02-01 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
EP1639091B1 (en) 2003-06-17 2012-12-05 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
CN101370819A (zh) 2003-07-02 2009-02-18 维莱尼姆公司 葡聚糖酶,编码它们的核酸以及制备和使用它们的方法
US7741089B2 (en) 2003-08-11 2010-06-22 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
US7435570B2 (en) 2003-08-11 2008-10-14 California Institute Of Technology Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
CA2734561A1 (en) 2004-06-09 2005-12-29 Pioneer Hi-Bred International, Inc. Plastid transit peptides
AU2005264938B2 (en) 2004-06-16 2011-11-24 Verenium Corporation Compositions and methods for enzymatic decolorization of chlorophyll
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
DK1861506T3 (en) 2005-03-15 2015-07-20 Bp Corp North America Inc Cellulases, nucleic acids encoding them, and methods of making and using them
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
CN100366752C (zh) * 2005-12-28 2008-02-06 河北省农林科学院遗传生理研究所 一种利用原子变构衍生dna序列多样性的方法
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
DK1989301T3 (en) 2006-02-10 2015-01-05 Bp Corp North America Inc Cellulolytic enzymes, nucleic acids encoding them, and methods for making and using them
DK1989302T3 (en) 2006-02-14 2018-07-23 Bp Corp North America Inc XYLANASES, NUCLEIC ACID CODING THEM AND PROCEDURES FOR THEIR PREPARATION AND USE
ES2416104T3 (es) 2006-03-07 2013-07-30 Cargill, Incorporated Aldolasas, ácidos nucleicos que las codifican y métodos para producir y usar las mismas
AU2007223086B2 (en) 2006-03-07 2013-09-26 Basf Enzymes Llc Aldolases, nucleic acids encoding them and methods for making and using them
WO2008016709A2 (en) 2006-08-04 2008-02-07 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EP2069389B1 (en) 2006-08-04 2014-10-08 BP Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
DK2057178T3 (da) 2006-09-21 2014-02-03 Verenium Corp Phytaser, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og benytte dem
EA015591B1 (ru) 2006-09-21 2011-10-31 Верениум Корпорейшн Фосфолипазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
HUE033455T2 (en) 2006-12-21 2017-12-28 Basf Enzymes Llc Amylases and glucoamylases, nucleic acids encoding them, and methods for their preparation and use
AU2008210495B2 (en) 2007-01-30 2014-02-27 Bp Corporation North America, Inc. Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
USRE46733E1 (en) 2007-10-03 2018-02-27 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
CA2710922A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
WO2009143397A2 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
CN102177236B (zh) * 2008-08-08 2013-11-06 东曹株式会社 功能改善的rna聚合酶突变体
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
CN102227503B (zh) 2008-09-26 2015-10-21 托卡根公司 重组载体
AU2010249500B2 (en) 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
WO2011127405A1 (en) 2010-04-08 2011-10-13 Sanford-Burnham Medical Research Institute Methods and compositions for enhanced delivery of compounds
US9187762B2 (en) 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
CN102560688B (zh) * 2010-12-15 2013-11-20 深圳华大基因科技服务有限公司 一种新的基于illumina测序平台的文库构建方法
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
BR112014027468A2 (pt) 2012-05-04 2017-06-27 Du Pont polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
EP2912169A4 (en) 2012-10-29 2016-11-23 Univ Rochester ARTEMISININE DERIVATIVES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE AS ANTIMALARIAMIDAL
BR112015023286A2 (pt) 2013-03-14 2018-03-06 Arzeda Corp polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase
EP2970995A1 (en) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
EA030896B1 (ru) 2013-08-16 2018-10-31 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CA3223359A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3087088B1 (en) 2013-12-23 2022-05-25 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
RU2021113662A (ru) 2014-02-07 2021-05-31 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CA2938979C (en) 2014-02-07 2024-02-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX381370B (es) 2014-10-16 2025-03-12 Corteva Agriscience Llc Proteinas insecticidas y metodos de uso.
RU2740312C2 (ru) 2015-01-15 2021-01-13 Пайониэ Хай-Бред Интернешнл, Инк. Инсектицидные белки и способы их применения
CA2977026A1 (en) 2015-03-11 2016-09-15 E.I. Du Pont De Nemours And Company Insecticidal combinations of pip-72 and methods of use
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017023486A1 (en) 2015-08-06 2017-02-09 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11008585B2 (en) 2016-05-04 2021-05-18 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3954202A1 (en) 2016-07-01 2022-02-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
EP3535285B1 (en) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112019012339A2 (pt) 2016-12-14 2019-11-26 Pioneer Hi Bred Int polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão
MX2019007491A (es) 2016-12-22 2019-09-06 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
WO2018148001A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use
US11555203B2 (en) 2017-05-11 2023-01-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
AU2019234562B2 (en) 2018-03-14 2024-08-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3092078A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US20230051453A1 (en) 2020-01-10 2023-02-16 The Regents Of The University Of California Biosynthetic platform for the production of olivetolic acid and analogues of olivetolic acid
WO2022015619A2 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5629179A (en) * 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
JP4156666B2 (ja) 1995-08-11 2008-09-24 ノボザイムス アクティーゼルスカブ ポリペプチド変異体を調整するための方法
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103539744A (zh) * 2013-10-17 2014-01-29 北京颖泰嘉和生物科技有限公司 一种制备3-(3,5-二氯苯基)-2,4-咪唑烷二酮的方法
CN103539744B (zh) * 2013-10-17 2016-03-23 北京颖泰嘉和生物科技股份有限公司 一种制备3-(3,5-二氯苯基)-2,4-咪唑烷二酮的方法
WO2016134521A1 (en) * 2015-02-27 2016-09-01 The University Of Hong Kong Dna display and methods thereof
CN108588050A (zh) * 2018-05-14 2018-09-28 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
CN117157148A (zh) * 2021-02-08 2023-12-01 胡桃夹子治疗公司 制备合成模板的方法
CN114197061A (zh) * 2022-02-17 2022-03-18 北京贝瑞和康生物技术有限公司 用于构建检测染色体拷贝数变异的测序文库的方法和试剂盒

Also Published As

Publication number Publication date
AU741834B2 (en) 2001-12-13
DK0973940T3 (da) 2008-10-20
EP0973940B1 (en) 2008-07-02
WO1998041653A1 (en) 1998-09-24
PL335860A1 (en) 2000-05-22
KR20000076363A (ko) 2000-12-26
AU6611598A (en) 1998-10-12
EP0973940A1 (en) 2000-01-26
ATE399880T1 (de) 2008-07-15
DE69839668D1 (de) 2008-08-14
JP2001515356A (ja) 2001-09-18
IL131966A0 (en) 2001-03-19
NZ337900A (en) 2001-05-25
BR9808368A (pt) 2000-05-23
CA2284253A1 (en) 1998-09-24

Similar Documents

Publication Publication Date Title
CN1249784A (zh) 一种体外构建dna文库的方法
JP4263248B2 (ja) Ｄｎａのシャッフリングによるライブラリーの作成方法
US6159687A (en) Methods for generating recombined polynucleotides
US6159688A (en) Methods of producing polynucleotide variants
CN1128875C (zh) 制备多肽变异体的方法
CN1197965C (zh) 在丝状真菌细胞内构建和筛选目的dna文库
CN1177376A (zh) 新的脂酶基因和用该基因产生脂酶的方法
CN1210557A (zh) 用于在细胞中体内产生突变体文库方法
US20150050658A1 (en) Gene shuffling methods
CN1276082C (zh) 合成cDNA的方法
CN1164868A (zh) 三肽基氨肽酶
CN1325445A (zh) 制备基因库的方法
CN1993475A (zh) 用dna文库转化和表达筛选丝状真菌细胞的方法
CN1483078A (zh) 合成单链核酸的方法
WO2002090536A2 (en) A method for producing recombined polynucleotides
CN100336906C (zh) 脂肪酶基因序列及其在酵母中的应用
CN1219063C (zh) 一种耐高温木聚糖酶和编码该酶的基因
CN1834236A (zh) 野生型枯草芽胞杆菌脂肪酶a变体及其应用
CN1153832C (zh) 一种耐热碱性磷酸酯酶及其表达
CN1371423A (zh) 编码环状脂肽酰基转移酶的基因及其表达
CN1802436A (zh) 具有t载体和表达载体功能的质粒以及采用其对靶基因的表达
CN1800401A (zh) 一种酯水解酶及其基因和重组酶
MXPA99008619A (en) An in vitro
CN1259416C (zh) 人DNA聚合酶β突变基因
CN1884533A (zh) 一种透明颤菌血红蛋白基因及其应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB02 Change of applicant information

Applicant after: Novo Nordisk A/S

Applicant before: Novo Nordisk A/S

COR Change of bibliographic data

Free format text: CORRECT: APPLICANT; FROM: NOVO NORDISK A/S TO: NOVOZYMES COMPANY

C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication