JP2001515356A - Dnaライブラリーの試験管内作製方法 - Google Patents
Dnaライブラリーの試験管内作製方法Info
- Publication number
- JP2001515356A JP2001515356A JP54004398A JP54004398A JP2001515356A JP 2001515356 A JP2001515356 A JP 2001515356A JP 54004398 A JP54004398 A JP 54004398A JP 54004398 A JP54004398 A JP 54004398A JP 2001515356 A JP2001515356 A JP 2001515356A
- Authority
- JP
- Japan
- Prior art keywords
- primer
- template
- polymerase
- dna
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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| PCT/DK1998/000104 WO1998041653A1 (en) | 1997-03-18 | 1998-03-18 | An in vitro method for construction of a dna library |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2002519038A (ja) * | 1998-06-29 | 2002-07-02 | フィロス インク. | 多様性の高いライブラリーを製造する方法 |
| JP5625908B2 (ja) * | 2008-08-08 | 2014-11-19 | 東ソー株式会社 | 機能改善したrnaポリメラーゼ変異体 |
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| US5965408A (en) * | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
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1998
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- 1998-03-18 IL IL13196698A patent/IL131966A0/xx unknown
- 1998-03-18 AT AT98907911T patent/ATE399880T1/de not_active IP Right Cessation
- 1998-03-18 CN CN98802958A patent/CN1249784A/zh active Pending
- 1998-03-18 BR BR9808368-6A patent/BR9808368A/pt not_active IP Right Cessation
- 1998-03-18 CA CA002284253A patent/CA2284253A1/en not_active Abandoned
- 1998-03-18 EP EP98907911A patent/EP0973940B1/en not_active Expired - Lifetime
- 1998-03-18 PL PL98335860A patent/PL335860A1/xx unknown
- 1998-03-18 DK DK98907911T patent/DK0973940T3/da active
- 1998-03-18 JP JP54004398A patent/JP2001515356A/ja not_active Ceased
- 1998-03-18 NZ NZ337900A patent/NZ337900A/en unknown
- 1998-03-18 KR KR1019997008465A patent/KR20000076363A/ko not_active Withdrawn
- 1998-03-18 WO PCT/DK1998/000104 patent/WO1998041653A1/en not_active Ceased
- 1998-03-18 AU AU66115/98A patent/AU741834B2/en not_active Ceased
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002519038A (ja) * | 1998-06-29 | 2002-07-02 | フィロス インク. | 多様性の高いライブラリーを製造する方法 |
| JP5625908B2 (ja) * | 2008-08-08 | 2014-11-19 | 東ソー株式会社 | 機能改善したrnaポリメラーゼ変異体 |
Also Published As
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|---|---|
| AU741834B2 (en) | 2001-12-13 |
| DK0973940T3 (da) | 2008-10-20 |
| EP0973940B1 (en) | 2008-07-02 |
| WO1998041653A1 (en) | 1998-09-24 |
| PL335860A1 (en) | 2000-05-22 |
| KR20000076363A (ko) | 2000-12-26 |
| AU6611598A (en) | 1998-10-12 |
| EP0973940A1 (en) | 2000-01-26 |
| ATE399880T1 (de) | 2008-07-15 |
| DE69839668D1 (de) | 2008-08-14 |
| CN1249784A (zh) | 2000-04-05 |
| IL131966A0 (en) | 2001-03-19 |
| NZ337900A (en) | 2001-05-25 |
| BR9808368A (pt) | 2000-05-23 |
| CA2284253A1 (en) | 1998-09-24 |
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