AU741834B2 - An (in vitro) method for construction of a dna library - Google Patents
An (in vitro) method for construction of a dna library Download PDFInfo
- Publication number
- AU741834B2 AU741834B2 AU66115/98A AU6611598A AU741834B2 AU 741834 B2 AU741834 B2 AU 741834B2 AU 66115/98 A AU66115/98 A AU 66115/98A AU 6611598 A AU6611598 A AU 6611598A AU 741834 B2 AU741834 B2 AU 741834B2
- Authority
- AU
- Australia
- Prior art keywords
- primers
- polynucleotide
- polynucleotides
- shuffled
- starting substrates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims abstract description 236
- 238000000338 in vitro Methods 0.000 title abstract description 11
- 238000010276 construction Methods 0.000 title abstract description 5
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 158
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 158
- 239000002157 polynucleotide Substances 0.000 claims abstract description 157
- 238000006243 chemical reaction Methods 0.000 claims abstract description 38
- 229920001184 polypeptide Polymers 0.000 claims description 81
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 81
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 81
- 108090000623 proteins and genes Proteins 0.000 claims description 58
- 239000012634 fragment Substances 0.000 claims description 51
- 239000000758 substrate Substances 0.000 claims description 47
- 108020004414 DNA Proteins 0.000 claims description 42
- 239000013598 vector Substances 0.000 claims description 31
- 102000004190 Enzymes Human genes 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 26
- 229940088598 enzyme Drugs 0.000 claims description 26
- 238000012216 screening Methods 0.000 claims description 23
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 18
- 108090001060 Lipase Proteins 0.000 claims description 18
- 102000004882 Lipase Human genes 0.000 claims description 16
- 239000004367 Lipase Substances 0.000 claims description 16
- 235000019421 lipase Nutrition 0.000 claims description 16
- 230000001747 exhibiting effect Effects 0.000 claims description 15
- 239000002773 nucleotide Substances 0.000 claims description 15
- 125000003729 nucleotide group Chemical group 0.000 claims description 15
- 108010017826 DNA Polymerase I Proteins 0.000 claims description 14
- 102000004594 DNA Polymerase I Human genes 0.000 claims description 14
- 108091005804 Peptidases Proteins 0.000 claims description 12
- 239000004365 Protease Substances 0.000 claims description 12
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 12
- 108010065511 Amylases Proteins 0.000 claims description 11
- 102000013142 Amylases Human genes 0.000 claims description 11
- 241000588724 Escherichia coli Species 0.000 claims description 11
- 102000004316 Oxidoreductases Human genes 0.000 claims description 11
- 108090000854 Oxidoreductases Proteins 0.000 claims description 11
- 235000019418 amylase Nutrition 0.000 claims description 11
- 230000010076 replication Effects 0.000 claims description 11
- 229960002685 biotin Drugs 0.000 claims description 9
- 235000020958 biotin Nutrition 0.000 claims description 9
- 239000011616 biotin Substances 0.000 claims description 9
- 230000002255 enzymatic effect Effects 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 102000014150 Interferons Human genes 0.000 claims description 8
- 108010050904 Interferons Proteins 0.000 claims description 8
- 229940079322 interferon Drugs 0.000 claims description 8
- 239000004382 Amylase Substances 0.000 claims description 7
- 230000000692 anti-sense effect Effects 0.000 claims description 7
- 229940088597 hormone Drugs 0.000 claims description 7
- 239000005556 hormone Substances 0.000 claims description 7
- 241000894007 species Species 0.000 claims description 7
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 102000003951 Erythropoietin Human genes 0.000 claims description 6
- 108090000394 Erythropoietin Proteins 0.000 claims description 6
- 108090000371 Esterases Proteins 0.000 claims description 6
- 108060003199 Glucagon Proteins 0.000 claims description 6
- 108010051696 Growth Hormone Proteins 0.000 claims description 6
- 102000004877 Insulin Human genes 0.000 claims description 6
- 108090001061 Insulin Proteins 0.000 claims description 6
- 102000009151 Luteinizing Hormone Human genes 0.000 claims description 6
- 108010073521 Luteinizing Hormone Proteins 0.000 claims description 6
- 102000003946 Prolactin Human genes 0.000 claims description 6
- 108010057464 Prolactin Proteins 0.000 claims description 6
- 102000003743 Relaxin Human genes 0.000 claims description 6
- 108090000103 Relaxin Proteins 0.000 claims description 6
- 102000007501 Thymosin Human genes 0.000 claims description 6
- 108010046075 Thymosin Proteins 0.000 claims description 6
- 102000011923 Thyrotropin Human genes 0.000 claims description 6
- 108010061174 Thyrotropin Proteins 0.000 claims description 6
- 102000002852 Vasopressins Human genes 0.000 claims description 6
- 108010004977 Vasopressins Proteins 0.000 claims description 6
- 108010089934 carbohydrase Proteins 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- 229940105423 erythropoietin Drugs 0.000 claims description 6
- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 claims description 6
- 229960004666 glucagon Drugs 0.000 claims description 6
- 229940125396 insulin Drugs 0.000 claims description 6
- 229940040129 luteinizing hormone Drugs 0.000 claims description 6
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims description 6
- 229940097325 prolactin Drugs 0.000 claims description 6
- LCJVIYPJPCBWKS-NXPQJCNCSA-N thymosin Chemical compound SC[C@@H](N)C(=O)N[C@H](CO)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CO)C(=O)N[C@H](CO)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H]([C@@H](C)CC)C(=O)N[C@H]([C@H](C)O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@H](CCC(O)=O)C(O)=O LCJVIYPJPCBWKS-NXPQJCNCSA-N 0.000 claims description 6
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 5
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 5
- 102000003982 Parathyroid hormone Human genes 0.000 claims description 5
- 108090000445 Parathyroid hormone Proteins 0.000 claims description 5
- 108091081021 Sense strand Proteins 0.000 claims description 5
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 239000000199 parathyroid hormone Substances 0.000 claims description 5
- 229960001319 parathyroid hormone Drugs 0.000 claims description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 4
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims description 4
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 4
- 102000013275 Somatomedins Human genes 0.000 claims description 4
- 102000005157 Somatostatin Human genes 0.000 claims description 4
- 108010056088 Somatostatin Proteins 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims description 4
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims description 4
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims description 4
- 239000002303 hypothalamus releasing factor Substances 0.000 claims description 4
- 230000006872 improvement Effects 0.000 claims description 4
- NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 claims description 4
- 229960000553 somatostatin Drugs 0.000 claims description 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 3
- 239000013604 expression vector Substances 0.000 claims description 3
- 101800000414 Corticotropin Proteins 0.000 claims description 2
- 239000000055 Corticotropin-Releasing Hormone Substances 0.000 claims description 2
- 102100027467 Pro-opiomelanocortin Human genes 0.000 claims description 2
- IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 claims description 2
- 229960000258 corticotropin Drugs 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 102000051325 Glucagon Human genes 0.000 claims 5
- 102000018997 Growth Hormone Human genes 0.000 claims 5
- 102000004157 Hydrolases Human genes 0.000 claims 5
- 108090000604 Hydrolases Proteins 0.000 claims 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 5
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims 5
- 230000001737 promoting effect Effects 0.000 claims 3
- 230000005026 transcription initiation Effects 0.000 claims 3
- 230000005030 transcription termination Effects 0.000 claims 3
- 230000000052 comparative effect Effects 0.000 claims 2
- 230000002267 hypothalamic effect Effects 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 239000003488 releasing hormone Substances 0.000 claims 2
- 230000014621 translational initiation Effects 0.000 claims 2
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 claims 1
- 101100166829 Mus musculus Cenpk gene Proteins 0.000 claims 1
- 101710150104 Sensory rhodopsin-1 Proteins 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract description 15
- 230000008569 process Effects 0.000 abstract description 15
- 238000003786 synthesis reaction Methods 0.000 abstract description 15
- 238000000137 annealing Methods 0.000 abstract description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 description 38
- 102220644676 Galectin-related protein_D96L_mutation Human genes 0.000 description 29
- 102200121669 rs104894915 Human genes 0.000 description 27
- 102000004169 proteins and genes Human genes 0.000 description 21
- 230000001976 improved effect Effects 0.000 description 20
- 238000005215 recombination Methods 0.000 description 20
- 230000006798 recombination Effects 0.000 description 20
- 235000018102 proteins Nutrition 0.000 description 19
- 230000035772 mutation Effects 0.000 description 14
- 239000000203 mixture Substances 0.000 description 10
- 102200105090 rs121909506 Human genes 0.000 description 9
- 102220126864 rs147455726 Human genes 0.000 description 9
- 239000011543 agarose gel Substances 0.000 description 8
- 150000001413 amino acids Chemical class 0.000 description 8
- 229940025131 amylases Drugs 0.000 description 8
- 238000002703 mutagenesis Methods 0.000 description 8
- 231100000350 mutagenesis Toxicity 0.000 description 8
- 102000053602 DNA Human genes 0.000 description 7
- 102000035195 Peptidases Human genes 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 7
- 239000003599 detergent Substances 0.000 description 7
- 230000004071 biological effect Effects 0.000 description 6
- 102220360655 c.170A>G Human genes 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 238000002708 random mutagenesis Methods 0.000 description 6
- 102220598266 Cancer/testis antigen family 45 member A10_C36A_mutation Human genes 0.000 description 5
- 108010084185 Cellulases Proteins 0.000 description 5
- 102000005575 Cellulases Human genes 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 230000037429 base substitution Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 5
- 102200088128 rs3213422 Human genes 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 230000006820 DNA synthesis Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 101100055551 Bacillus licheniformis amyS gene Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 241000055915 Heterocoma lanuginosa Species 0.000 description 3
- 101100216056 Lactobacillus amylovorus amyL gene Proteins 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- -1 amyL Chemical class 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000000977 initiatory effect Effects 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 108010011619 6-Phytase Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 2
- 108010029541 Laccase Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000007801 affinity label Substances 0.000 description 2
- 101150039703 amyL gene Proteins 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004851 dishwashing Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000007423 screening assay Methods 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
- 102000003849 Cytochrome P450 Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 102400000321 Glucagon Human genes 0.000 description 1
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 101710130181 Protochlorophyllide reductase A, chloroplastic Proteins 0.000 description 1
- 102000001218 Rec A Recombinases Human genes 0.000 description 1
- 108010055016 Rec A Recombinases Proteins 0.000 description 1
- 102100038803 Somatotropin Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 108090000787 Subtilisin Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000003348 filter assay Methods 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 102220330036 rs1555864368 Human genes 0.000 description 1
- 102200028671 rs3765620 Human genes 0.000 description 1
- 102200026915 rs776679653 Human genes 0.000 description 1
- 102220098472 rs878853116 Human genes 0.000 description 1
- 102220410746 rs886044165 Human genes 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000012409 standard PCR amplification Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK307/97 | 1997-03-18 | ||
| DK30797 | 1997-03-18 | ||
| DK434/97 | 1997-04-17 | ||
| DK43497 | 1997-04-17 | ||
| DK625/97 | 1997-05-30 | ||
| DK62597 | 1997-05-30 | ||
| PCT/DK1998/000104 WO1998041653A1 (en) | 1997-03-18 | 1998-03-18 | An in vitro method for construction of a dna library |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU6611598A AU6611598A (en) | 1998-10-12 |
| AU741834B2 true AU741834B2 (en) | 2001-12-13 |
Family
ID=27220576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU66115/98A Ceased AU741834B2 (en) | 1997-03-18 | 1998-03-18 | An (in vitro) method for construction of a dna library |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP0973940B1 (enExample) |
| JP (1) | JP2001515356A (enExample) |
| KR (1) | KR20000076363A (enExample) |
| CN (1) | CN1249784A (enExample) |
| AT (1) | ATE399880T1 (enExample) |
| AU (1) | AU741834B2 (enExample) |
| BR (1) | BR9808368A (enExample) |
| CA (1) | CA2284253A1 (enExample) |
| DE (1) | DE69839668D1 (enExample) |
| DK (1) | DK0973940T3 (enExample) |
| IL (1) | IL131966A0 (enExample) |
| NZ (1) | NZ337900A (enExample) |
| PL (1) | PL335860A1 (enExample) |
| WO (1) | WO1998041653A1 (enExample) |
Families Citing this family (131)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US20060257890A1 (en) | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| WO1999029902A1 (en) | 1997-12-08 | 1999-06-17 | California Institute Of Technology | Method for creating polynucleotide and polypeptide sequences |
| US7390619B1 (en) | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| EP1092039B1 (en) * | 1998-06-29 | 2012-02-29 | Bristol-Myers Squibb Company | Methods for generating highly diverse libraries |
| WO2000009682A1 (en) | 1998-08-12 | 2000-02-24 | Maxygen, Inc. | Dna shuffling of monooxygenase genes for production of industrial chemicals |
| WO2000020573A2 (en) | 1998-10-07 | 2000-04-13 | Maxygen, Inc. | Dna shuffling to produce nucleic acids for mycotoxin detoxification |
| CN1197965C (zh) | 1998-10-26 | 2005-04-20 | 诺维信公司 | 在丝状真菌细胞内构建和筛选目的dna文库 |
| EP1129184A1 (en) | 1998-11-10 | 2001-09-05 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes |
| CA2353379C (en) * | 1998-11-27 | 2011-01-04 | Novozymes A/S | Lipolytic enzyme variants |
| US7312062B2 (en) | 1998-11-27 | 2007-12-25 | Novozymes A/S | Lipolytic enzyme variants |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| WO2000042560A2 (en) | 1999-01-19 | 2000-07-20 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| IL144657A0 (en) | 1999-02-11 | 2002-06-30 | Maxygen Inc | High throughput mass spectrometry |
| US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
| JP2003509010A (ja) * | 1999-05-31 | 2003-03-11 | ノボザイムス アクティーゼルスカブ | ペプチドのスクリーニング方法 |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
| US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
| IL150291A0 (en) | 2000-01-11 | 2002-12-01 | Maxygen Inc | Integrated systems and methods for diversity generation and screening |
| US6248541B1 (en) | 2000-04-21 | 2001-06-19 | Genencor International, Inc. | Screening under nutrient limited conditions |
| US6534292B1 (en) | 2000-05-08 | 2003-03-18 | Genencor International, Inc. | Methods for forming recombined nucleic acids |
| US7115403B1 (en) | 2000-05-16 | 2006-10-03 | The California Institute Of Technology | Directed evolution of galactose oxidase enzymes |
| AU2001268716A1 (en) | 2000-06-23 | 2002-01-08 | Maxygen, Inc. | Novel chimeric promoters |
| AU2001272978A1 (en) | 2000-06-23 | 2002-01-08 | Maxygen, Inc. | Novel co-stimulatory molecules |
| US6994963B1 (en) * | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
| US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
| AUPQ974900A0 (en) | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
| US6582914B1 (en) | 2000-10-26 | 2003-06-24 | Genencor International, Inc. | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
| AU2002220529A1 (en) * | 2000-12-06 | 2002-06-18 | Novozymes A/S | Method for producing a polynucleotide library |
| US6783941B2 (en) | 2000-12-06 | 2004-08-31 | Novozymes A/S | Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| US7465567B2 (en) | 2001-04-16 | 2008-12-16 | California Institute Of Technology | Peroxide-driven cytochrome P450 oxygenase variants |
| WO2003008563A2 (en) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Improved cytochrome p450 oxygenases |
| AU2003216507A1 (en) | 2002-03-01 | 2003-09-16 | Maxygen, Inc. | Methods, systems, and software for identifying functional bio-molecules |
| AU2003213846A1 (en) | 2002-03-09 | 2003-09-29 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
| EA010903B1 (ru) | 2002-04-19 | 2008-12-30 | Дайверса Корпорейшн | Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| DK1504098T4 (da) | 2002-05-17 | 2011-05-23 | Alligator Bioscience Ab | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
| US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| MXPA05001287A (es) | 2002-08-06 | 2005-08-03 | Verdia Llc | Variantes de amino oxidasa ap1. |
| WO2004016811A2 (en) * | 2002-08-19 | 2004-02-26 | Danmarks Tekniske Universitet (Dtu) | Methods and kit for genes shuffling using tagged primers |
| CA2920416A1 (en) | 2003-03-06 | 2004-10-28 | Basf Enzymes Llc | Amylases, nucleic acids encoding them and methods for making and using them |
| MX295545B (es) | 2003-03-07 | 2012-02-03 | Diversa Corp | Hidrolasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas. |
| DK1613733T3 (en) | 2003-04-04 | 2015-08-31 | Basf Enzymes Llc | PEKTATLYASER, nucleic acids encoding them, and methods for making and using the |
| JP2007500514A (ja) | 2003-04-29 | 2007-01-18 | パイオニア ハイ−ブレッド インターナショナル, インコーポレイテッド | 新規なグリホセートn−アセチルトランスフェラーゼ(gat)遺伝子 |
| WO2005017105A2 (en) | 2003-06-17 | 2005-02-24 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
| BRPI0412279A (pt) | 2003-07-02 | 2006-09-19 | Diversa Corp | glucanases, ácidos nucléicos codificando as mesmas e métodos para preparar e aplicar os mesmos |
| US7435570B2 (en) | 2003-08-11 | 2008-10-14 | California Institute Of Technology | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
| CA2535526C (en) | 2003-08-11 | 2015-09-29 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
| CA2735315A1 (en) | 2004-06-09 | 2005-12-29 | Pioneer Hi-Bred International, Inc. | Plastid transit peptides |
| EP2468853B1 (en) | 2004-06-16 | 2015-04-01 | DSM IP Assets B.V. | Method for enzymatic decolorization of pheophytin |
| CA2614769A1 (en) | 2005-03-10 | 2006-09-21 | Verenium Corporation | Lyase enzymes, nucleic acids encoding them and methods for making and using them |
| CN102943085A (zh) | 2005-03-15 | 2013-02-27 | 维莱尼姆公司 | 纤维素酶、编码它们的核酸及其制备和应用的方法 |
| US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| CN100366752C (zh) * | 2005-12-28 | 2008-02-06 | 河北省农林科学院遗传生理研究所 | 一种利用原子变构衍生dna序列多样性的方法 |
| EP2216403A3 (en) | 2006-02-02 | 2010-11-24 | Verenium Corporation | Esterases and related nucleic acids and methods |
| NZ595497A (en) | 2006-02-10 | 2013-09-27 | Verenium Corp | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
| EP2548954A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
| EP1999256B1 (en) | 2006-03-07 | 2012-03-07 | Verenium Corporation | Aldolases, nucleic acids encoding them and methods for making and using them |
| AU2007223913B2 (en) | 2006-03-07 | 2013-06-13 | Cargill, Incorporated | Aldolases, nucleic acids encoding them and methods for making and using them |
| US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| WO2008016709A2 (en) | 2006-08-04 | 2008-02-07 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| MX369001B (es) | 2006-08-04 | 2019-10-24 | Basf Enzymes Llc | Glucanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas. |
| PL2057274T3 (pl) | 2006-09-21 | 2014-05-30 | Dsm Ip Assets Bv | Fosfolipazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i stosowania |
| EP2617823B1 (en) | 2006-09-21 | 2015-07-01 | BASF Enzymes LLC | Phytases, nucleic acids encoding them and methods for making and using them |
| ES2575912T3 (es) | 2006-12-21 | 2016-07-04 | Basf Enzymes Llc | Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas |
| WO2008095033A2 (en) | 2007-01-30 | 2008-08-07 | Verenium Corporation | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
| NZ584967A (en) | 2007-10-03 | 2012-08-31 | Verenium Corp | Xylanases, nucleic acids encoding them and methods for making and using them |
| US8541220B2 (en) | 2008-01-03 | 2013-09-24 | Verenium Corporation | Isomerases, nucleic acids encoding them and methods for making and using them |
| PL2238242T3 (pl) | 2008-01-03 | 2015-06-30 | Basf Enzymes Llc | Transferazy i oksydoreduktazy, kwasy nukleinowe kodujące je oraz sposoby ich wytwarzania i zastosowania |
| WO2009143397A2 (en) | 2008-05-23 | 2009-11-26 | E. I. Du Pont De Nemours And Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| KR20110044753A (ko) * | 2008-08-08 | 2011-04-29 | 토소가부시키가이샤 | 기능 개선된 rna 중합효소 변이체 |
| US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| CN105274125A (zh) | 2008-09-26 | 2016-01-27 | 托卡根公司 | 基因治疗载体和胞嘧啶脱氨酶 |
| BRPI1011160A8 (pt) | 2009-05-21 | 2018-01-02 | Verenium Corp | Fitases, preparação de proteína que as compreende, e seus usos |
| UA111708C2 (uk) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
| UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
| JP2013525285A (ja) | 2010-04-08 | 2013-06-20 | サンフォード−バーナム メディカル リサーチ インスティテュート | 化合物の送達を増強するための方法および組成物 |
| CA2805937A1 (en) | 2010-08-13 | 2012-02-16 | Pioneer Hi-Bred International, Inc. | Chimeric promoters and methods of use |
| CN102560688B (zh) * | 2010-12-15 | 2013-11-20 | 深圳华大基因科技服务有限公司 | 一种新的基于illumina测序平台的文库构建方法 |
| US9322007B2 (en) | 2011-07-22 | 2016-04-26 | The California Institute Of Technology | Stable fungal Cel6 enzyme variants |
| CA2872096A1 (en) | 2012-05-04 | 2013-11-07 | E. I. Du Pont De Nemours And Company | Compositions and methods comprising sequences having meganuclease activity |
| WO2014070673A1 (en) | 2012-10-29 | 2014-05-08 | University Of Rochester | Artemisinin derivatives, methods for their preparation and their use as antimalarial agents |
| US20140287419A1 (en) | 2013-03-14 | 2014-09-25 | Arzeda Corp. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
| EP2970935A1 (en) | 2013-03-14 | 2016-01-20 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
| WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
| EP3032942B1 (en) | 2013-08-16 | 2020-03-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CA2923726C (en) | 2013-09-13 | 2024-01-30 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN103539744B (zh) * | 2013-10-17 | 2016-03-23 | 北京颖泰嘉和生物科技股份有限公司 | 一种制备3-(3,5-二氯苯基)-2,4-咪唑烷二酮的方法 |
| EP3087088B1 (en) | 2013-12-23 | 2022-05-25 | University Of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
| BR112016018103B1 (pt) | 2014-02-07 | 2024-01-16 | E.I. Du Pont De Nemours And Company | Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto |
| CN106536545A (zh) | 2014-02-07 | 2017-03-22 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| US10435706B2 (en) | 2014-10-16 | 2019-10-08 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US10231460B2 (en) | 2015-01-15 | 2019-03-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| WO2016134521A1 (en) * | 2015-02-27 | 2016-09-01 | The University Of Hong Kong | Dna display and methods thereof |
| MX376048B (es) | 2015-03-11 | 2025-03-07 | Corteva Agriscience Llc | Combinaciones insecticidas de pip-72 y métodos de uso. |
| WO2016186986A1 (en) | 2015-05-19 | 2016-11-24 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
| US11198709B2 (en) | 2015-08-06 | 2021-12-14 | E. I. Du Pont De Nemours And Company | Plant derived insecticidal proteins and methods for their use |
| US20180325119A1 (en) | 2015-12-18 | 2018-11-15 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US11008585B2 (en) | 2016-05-04 | 2021-05-18 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| PH12018502706B1 (en) | 2016-07-01 | 2024-05-24 | Pioneer Hi Bred Int | Insecticidal proteins from plants and methods for their use |
| CN109863167B (zh) | 2016-11-01 | 2022-12-23 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| US11174295B2 (en) | 2016-12-14 | 2021-11-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CA3046226A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| WO2018148001A1 (en) | 2017-02-08 | 2018-08-16 | Pioneer Hi-Bred International Inc | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
| BR112019023628A2 (pt) | 2017-05-11 | 2020-06-02 | Pioneer Hi-Bred International, Inc. | Polipeptídeo inseticida recombinante, proteína inseticida quimérica, proteína de fusão, composição agrícola, polinucleotídeo recombinante, construto de dna, planta transgênica, método para inibir o crescimento ou exterminar uma praga de inseto ou população de praga, método para controlar os danos por praga de inseto, método para controlar infestação de praga e método para melhorar o rendimento de uma cultura |
| WO2019040335A1 (en) | 2017-08-19 | 2019-02-28 | University Of Rochester | MICHELOLID DERIVATIVES, PREPARATION METHODS AND THEIR USE AS ANTICANCER AND ANTI-INFLAMMATORY AGENTS |
| MX2020009435A (es) | 2018-03-14 | 2020-11-11 | Pioneer Hi Bred Int | Proteinas con actividad insecticida de plantas y metodos para sus usos. |
| EP4659582A2 (en) | 2018-03-14 | 2025-12-10 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| CN112941155B (zh) * | 2018-05-14 | 2023-07-14 | 北京艾克伦医疗科技有限公司 | 具有茎环结构的dna引物对及其应用 |
| CN115151643A (zh) | 2020-01-10 | 2022-10-04 | 加利福尼亚大学董事会 | 用于产生橄榄醇酸和橄榄醇酸类似物的生物合成平台 |
| WO2022015619A2 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| NL2027654B1 (en) * | 2021-02-08 | 2022-09-09 | Nutcracker Therapeutics Inc | Methods for manufacturing a syntetic template |
| CN114197061A (zh) * | 2022-02-17 | 2022-03-18 | 北京贝瑞和康生物技术有限公司 | 用于构建检测染色体拷贝数变异的测序文库的方法和试剂盒 |
| WO2023173084A1 (en) | 2022-03-11 | 2023-09-14 | University Of Rochester | Cyclopeptibodies and uses thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3662697A (en) * | 1996-07-09 | 1998-02-02 | Bp Corporation North America Inc. | Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5629179A (en) * | 1995-03-17 | 1997-05-13 | Novagen, Inc. | Method and kit for making CDNA library |
| JP4156666B2 (ja) | 1995-08-11 | 2008-09-24 | ノボザイムス アクティーゼルスカブ | ポリペプチド変異体を調整するための方法 |
-
1998
- 1998-03-18 EP EP98907911A patent/EP0973940B1/en not_active Expired - Lifetime
- 1998-03-18 CA CA002284253A patent/CA2284253A1/en not_active Abandoned
- 1998-03-18 IL IL13196698A patent/IL131966A0/xx unknown
- 1998-03-18 AT AT98907911T patent/ATE399880T1/de not_active IP Right Cessation
- 1998-03-18 WO PCT/DK1998/000104 patent/WO1998041653A1/en not_active Ceased
- 1998-03-18 NZ NZ337900A patent/NZ337900A/en unknown
- 1998-03-18 DE DE69839668T patent/DE69839668D1/de not_active Expired - Lifetime
- 1998-03-18 CN CN98802958A patent/CN1249784A/zh active Pending
- 1998-03-18 PL PL98335860A patent/PL335860A1/xx unknown
- 1998-03-18 BR BR9808368-6A patent/BR9808368A/pt not_active IP Right Cessation
- 1998-03-18 DK DK98907911T patent/DK0973940T3/da active
- 1998-03-18 AU AU66115/98A patent/AU741834B2/en not_active Ceased
- 1998-03-18 JP JP54004398A patent/JP2001515356A/ja not_active Ceased
- 1998-03-18 KR KR1019997008465A patent/KR20000076363A/ko not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3662697A (en) * | 1996-07-09 | 1998-02-02 | Bp Corporation North America Inc. | Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process |
Also Published As
| Publication number | Publication date |
|---|---|
| DK0973940T3 (da) | 2008-10-20 |
| PL335860A1 (en) | 2000-05-22 |
| AU6611598A (en) | 1998-10-12 |
| WO1998041653A1 (en) | 1998-09-24 |
| DE69839668D1 (de) | 2008-08-14 |
| JP2001515356A (ja) | 2001-09-18 |
| CA2284253A1 (en) | 1998-09-24 |
| ATE399880T1 (de) | 2008-07-15 |
| EP0973940B1 (en) | 2008-07-02 |
| IL131966A0 (en) | 2001-03-19 |
| EP0973940A1 (en) | 2000-01-26 |
| CN1249784A (zh) | 2000-04-05 |
| NZ337900A (en) | 2001-05-25 |
| BR9808368A (pt) | 2000-05-23 |
| KR20000076363A (ko) | 2000-12-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU741834B2 (en) | An (in vitro) method for construction of a dna library | |
| US6159687A (en) | Methods for generating recombined polynucleotides | |
| US6159688A (en) | Methods of producing polynucleotide variants | |
| EP0996718B1 (en) | Method for constructing a library using dna shuffling | |
| EP1015575B1 (en) | Shuffling of heterologous dna sequences | |
| Sen et al. | Developments in directed evolution for improving enzyme functions | |
| EP2009102A2 (en) | Random mutagenesis and amplification of nucleic acid | |
| JP6552969B2 (ja) | 定方向進化のためのライブラリーの作製方法 | |
| JPH04507346A (ja) | アルカリ性タンパク質分解酵素およびその製造方法 | |
| CN104789556A (zh) | 一种基于合成单链dna文库快速高效组合进化dna的方法 | |
| WO2002038757A1 (en) | Method for generating recombinant dna library using unidirectional single-stranded dna fragments | |
| US6291165B1 (en) | Shuffling of heterologous DNA sequences | |
| EP1263987B1 (en) | Random truncation and amplification of nucleic acid | |
| MXPA99008619A (en) | An in vitro | |
| EP1670932A2 (en) | Libraries of recombinant chimeric proteins | |
| Poluri et al. | Expanding the synthetic protein universe by guided evolutionary concepts | |
| CN106544331B (zh) | 一种新的定向进化技术snds及所获得耐热高效甲基对硫磷水解杂合酶 | |
| Shao et al. | Random-priming in vitro recombination: an effective | |
| Harayama | Effective DNA Shuffling Methods for Enzyme Evolution | |
| Jain et al. | MODERN STRATEGIES FOR ENHANCED CATALYTIC ACTIVITY OF ENZYMES | |
| MXPA99008622A (en) | Shuffling of heterologous dna sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1 | Assignment before grant (sect. 113) |
Owner name: NOVOZYMES A/S Free format text: THE FORMER OWNER WAS: NOVO-NORDISK A/S |
|
| FGA | Letters patent sealed or granted (standard patent) |