CN1154717C - 改良无限增殖化人类皮肤细胞系和用于其生产的新型无血清培育基 - Google Patents

改良无限增殖化人类皮肤细胞系和用于其生产的新型无血清培育基 Download PDF

Info

Publication number
CN1154717C
CN1154717C CNB961991755A CN96199175A CN1154717C CN 1154717 C CN1154717 C CN 1154717C CN B961991755 A CNB961991755 A CN B961991755A CN 96199175 A CN96199175 A CN 96199175A CN 1154717 C CN1154717 C CN 1154717C
Authority
CN
China
Prior art keywords
keratinocyte
substratum
keratinocytes
immortalization
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB961991755A
Other languages
English (en)
Chinese (zh)
Other versions
CN1205737A (zh
Inventor
M
M·鲍尔
C·马斯
��˹��ŵ��
A·马尔诺伊
A���շƷ�
A·M·A·普菲菲
M·雷尼尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe des Produits Nestle SA
Original Assignee
Societe des Produits Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe des Produits Nestle SA filed Critical Societe des Produits Nestle SA
Publication of CN1205737A publication Critical patent/CN1205737A/zh
Application granted granted Critical
Publication of CN1154717C publication Critical patent/CN1154717C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides or bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/81—Adrenaline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CNB961991755A 1995-12-21 1996-12-19 改良无限增殖化人类皮肤细胞系和用于其生产的新型无血清培育基 Expired - Fee Related CN1154717C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57648395A 1995-12-21 1995-12-21
US08/576,483 1995-12-21

Publications (2)

Publication Number Publication Date
CN1205737A CN1205737A (zh) 1999-01-20
CN1154717C true CN1154717C (zh) 2004-06-23

Family

ID=24304605

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB961991755A Expired - Fee Related CN1154717C (zh) 1995-12-21 1996-12-19 改良无限增殖化人类皮肤细胞系和用于其生产的新型无血清培育基

Country Status (23)

Country Link
US (2) US6423540B2 (https=)
EP (2) EP0780469B1 (https=)
JP (1) JP4404965B2 (https=)
KR (1) KR100455006B1 (https=)
CN (1) CN1154717C (https=)
AT (1) ATE199390T1 (https=)
AU (1) AU730222B2 (https=)
BR (1) BR9612256B1 (https=)
CA (1) CA2234118C (https=)
CZ (1) CZ297289B6 (https=)
DE (1) DE69611894T2 (https=)
DK (1) DK0780469T3 (https=)
ES (1) ES2155166T3 (https=)
GR (1) GR3035719T3 (https=)
HU (1) HU226195B1 (https=)
IL (1) IL124191A (https=)
NO (1) NO326190B1 (https=)
NZ (1) NZ325814A (https=)
PL (1) PL195108B1 (https=)
PT (1) PT780469E (https=)
RU (1) RU2215030C2 (https=)
SK (1) SK283314B6 (https=)
WO (1) WO1997023602A1 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU226195B1 (hu) * 1995-12-21 2008-06-30 Nestle Sa Javított immortalizált humán bõrsejtvonalak és szérrummentes tápközeg elõállításukra
NZ506944A (en) * 1998-04-17 2003-10-31 Nestle Sa Immortalised keratinocyte cell line derived from normal human skin tissues
US6964869B2 (en) 1998-07-13 2005-11-15 Wisconsin Alumni Research Foundation Method and composition for skin grafts
CN1087778C (zh) * 1999-03-02 2002-07-17 华东理工大学 杂交瘤细胞无血清培养基
DE19912798C1 (de) * 1999-03-10 2000-02-17 Andreas Jordan Verfahren zur Kultivierung von Krebszellen aus Humangewebe und Vorrichtung zur Aufbereitung von Gewebeproben
AUPR298901A0 (en) 2001-02-07 2001-03-08 McComb Foundation, Inc., The Cell suspension preparation technique and device
NZ529035A (en) * 2001-04-24 2007-01-26 Wisconsin Alumni Res Found Chimeric skin comprised of human immortalised keratinocyte cells and human donor cells for use in skin grafting and plastic surgery methods
US7262174B2 (en) 2001-05-09 2007-08-28 Geron Corporation Treatment for wounds
US20030022369A1 (en) * 2001-05-18 2003-01-30 Helen Fillmore Differentiation of specialized dermal and epidermal cells into neuronal cells
EP1417629B1 (en) * 2001-07-06 2008-02-20 Lipomics Technologies, Inc. Generating, viewing, interpreting, and utilizing a quantitative database of metabolites
GB0208041D0 (en) 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US20050025737A1 (en) * 2003-07-30 2005-02-03 Sebagh Jean Louis Compositions containing melon extracts
MXPA06003028A (es) * 2003-09-18 2006-06-23 Raven Biotechnologies Inc Medios de cultivo de celulas.
WO2005071065A1 (en) * 2004-01-26 2005-08-04 Cognis France S.A.S Method for studying functional interactions between sensory neurons and keratinocytes or melanocytes
US9295543B2 (en) 2005-03-17 2016-03-29 Stratatech Corporation Skin substitutes with improved purity
EP1818392B1 (en) * 2006-02-14 2010-08-25 Genetix Limited Cell culture medium
WO2010065907A2 (en) * 2008-12-05 2010-06-10 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
KR101768632B1 (ko) * 2011-05-18 2017-08-17 (주)아모레퍼시픽 칼슘 펄스를 통한 배양상피세포층의 분리방법
CN103797110B (zh) * 2011-07-12 2017-08-25 食品安全测试系统公司 沙门氏菌和大肠杆菌的培养基和培养方法以及用于检测沙门氏菌和大肠杆菌的方法
WO2013129885A1 (ko) * 2012-02-28 2013-09-06 건국대학교 산학협력단 세포 배양액
EP2975117B1 (en) 2013-03-11 2018-01-10 JCR Pharmaceuticals Co., Ltd. Method for producing human corneal epithelium sheet
AU2013205148B2 (en) 2013-03-14 2014-10-30 AVITA Medical Americas, LLC Systems and methods for tissue processing and preparation of cell suspension therefrom
KR20160065038A (ko) 2014-11-28 2016-06-08 (주)아모레퍼시픽 멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
US10280688B2 (en) * 2015-01-26 2019-05-07 Halliburton Energy Services, Inc. Rotating superhard cutting element
CN105238739A (zh) * 2015-11-11 2016-01-13 朱宁文 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) * 2016-12-24 2017-03-22 严志海 一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) * 2017-05-12 2017-09-01 青岛赛欧凯普图乐生物医药有限公司 一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) * 2020-07-31 2022-02-18 上海尚瑞生物医药科技有限公司 一种无血清的黑素母细胞培养液及其培养方法
KR102583179B1 (ko) * 2020-11-27 2023-09-26 (주)엑셀세라퓨틱스 칼슘, 상피세포성장인자 및 알부민을 포함하는 각질형성세포 수립 또는 배양용 배지 조성물
US12570949B2 (en) 2022-12-27 2026-03-10 AVITA Medical Americas, LLC Systems and devices for wound therapy and related methods of use
US12180456B2 (en) 2022-12-27 2024-12-31 AVITA Medical Americas, LLC Tissue healing
USD1112812S1 (en) 2023-12-22 2026-02-10 AVITA Medical Americas, LLC Tissue processing cartridge
CN119684410B (zh) * 2025-02-21 2025-04-25 深圳市容大生物科技股份有限公司 一种小分子多功能信息肽及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940666A (en) * 1983-07-15 1990-07-10 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
US4885238A (en) 1987-10-30 1989-12-05 The United States Of America As Represented By The Department Of Health And Human Services Immortalized human bronchial epitherial mesothelial cell lines
US5292655A (en) * 1990-01-29 1994-03-08 Wille Jr John J Method for the formation of a histologically-complete skin substitute
US5376542A (en) 1992-04-27 1994-12-27 Georgetown University Method for producing immortalized cell lines using human papilluma virus genes
WO1995027510A1 (en) * 1994-04-12 1995-10-19 Alza Corporation Screening methods for integumental inflammation modulating agents
DE19617261A1 (de) 1995-04-21 1996-11-28 Naeher Helmut Dr Med Priv Doz Hautverträglichkeitstest
HU226195B1 (hu) * 1995-12-21 2008-06-30 Nestle Sa Javított immortalizált humán bõrsejtvonalak és szérrummentes tápközeg elõállításukra

Also Published As

Publication number Publication date
SK283314B6 (sk) 2003-05-02
DE69611894T2 (de) 2001-09-13
EP0877797A1 (en) 1998-11-18
DK0780469T3 (da) 2001-03-26
US20020012993A1 (en) 2002-01-31
NO982810L (no) 1998-08-21
HUP9903742A3 (en) 2000-10-30
CZ194598A3 (cs) 1998-11-11
SK83598A3 (en) 1999-01-11
DE69611894D1 (de) 2001-04-05
US6949381B2 (en) 2005-09-27
ES2155166T3 (es) 2001-05-01
AU730222B2 (en) 2001-03-01
PL195108B1 (pl) 2007-08-31
NO326190B1 (no) 2008-10-13
EP0780469B1 (fr) 2001-02-28
CA2234118C (en) 2009-04-07
CN1205737A (zh) 1999-01-20
PT780469E (pt) 2001-06-29
KR19990071817A (ko) 1999-09-27
BR9612256A (pt) 1999-07-13
ATE199390T1 (de) 2001-03-15
WO1997023602A1 (en) 1997-07-03
JP2000506374A (ja) 2000-05-30
GR3035719T3 (en) 2001-07-31
CA2234118A1 (en) 1997-07-03
AU1305497A (en) 1997-07-17
RU2215030C2 (ru) 2003-10-27
EP0780469A1 (fr) 1997-06-25
JP4404965B2 (ja) 2010-01-27
IL124191A (en) 2004-06-01
US20020042129A1 (en) 2002-04-11
NO982810D0 (no) 1998-06-18
PL327320A1 (en) 1998-12-07
BR9612256B1 (pt) 2010-08-10
KR100455006B1 (ko) 2005-01-13
CZ297289B6 (cs) 2006-10-11
HUP9903742A2 (hu) 2000-03-28
HU226195B1 (hu) 2008-06-30
US6423540B2 (en) 2002-07-23
NZ325814A (en) 2000-10-27

Similar Documents

Publication Publication Date Title
CN1154717C (zh) 改良无限增殖化人类皮肤细胞系和用于其生产的新型无血清培育基
JP2000506374A5 (https=)
CN100347293C (zh) 来源于单核细胞的去分化的可程序化干细胞及其制备和应用
AU756151B2 (en) Immortalised cell lines derived from normal human skin tissues
CN1458973A (zh) 前脂肪细胞系
CN1649999A (zh) 用于生产目的物质的禽细胞系
CN1649998A (zh) 为研究、临床眼晴表面移植和组织工程的应用而生长人类结膜组织等同物的方法
CN1126489A (zh) 来自人骨髓的新的基质细胞系及其用途
CN1826406A (zh) 用附着或非附着鸟细胞系生产痘病毒
CN101052711A (zh) 制备星形胶质细胞样细胞的条件培养基的方法
US20080085554A1 (en) Culture Medium for Culturing Feeder Cells for Embryonic Stem Cells Culture and the Prepared Feeder Cells
HK1060592A (en) Pre-adipose cell lines
HK1038243A (en) Immortalised cell lines derived from normal human skin tissues

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20040623

Termination date: 20151219

EXPY Termination of patent right or utility model