ES2155166T3 - Lineas inmortalizadas de celulas derivadas de tejidos cutaneos humanos normales y medio de cultivo sin suero, adaptados a su cultivo. - Google Patents

Lineas inmortalizadas de celulas derivadas de tejidos cutaneos humanos normales y medio de cultivo sin suero, adaptados a su cultivo.

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Publication number
ES2155166T3
ES2155166T3 ES96203641T ES96203641T ES2155166T3 ES 2155166 T3 ES2155166 T3 ES 2155166T3 ES 96203641 T ES96203641 T ES 96203641T ES 96203641 T ES96203641 T ES 96203641T ES 2155166 T3 ES2155166 T3 ES 2155166T3
Authority
ES
Spain
Prior art keywords
serum
normal human
crop
fabrics
growth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96203641T
Other languages
English (en)
Inventor
Markus Baur
Catherine Mace
Armand Malnoe
Andrea M A Pfeifer
Marcelle Regnier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe des Produits Nestle SA
Nestle SA
Original Assignee
Societe des Produits Nestle SA
Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe des Produits Nestle SA, Nestle SA filed Critical Societe des Produits Nestle SA
Application granted granted Critical
Publication of ES2155166T3 publication Critical patent/ES2155166T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides or bases
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/81—Adrenaline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A PROLES CELULARES CONTINUOS (INMORTALIZADOS), EN PARTICULAR DE QUERATINOCITOS Y MELANOCITOS DERIVADOS DE UN TEJIDO CUTANEO HUMANO NORMAL, ASI COMO MEDIOS NUEVOS SIN SUERO PARA AISLAR, PRODUCIR Y MANTENER ESTAS PROLES. SE REFIERE TAMBIEN A PROCEDIMIENTOS PARA PRODUCIR MELANOCITOS Y QUERATINOCITOS PRIMARIOS EN AUSENCIA DE SUERO Y SIN CELULAS NUTRITIVAS. APLICACION: ANALISIS PERFECCIONADOS DE REACCIONES CUTANEAS Y EXPRESION DE GENES HETEROLOGOS.
ES96203641T 1995-12-21 1996-12-19 Lineas inmortalizadas de celulas derivadas de tejidos cutaneos humanos normales y medio de cultivo sin suero, adaptados a su cultivo. Expired - Lifetime ES2155166T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57648395A 1995-12-21 1995-12-21

Publications (1)

Publication Number Publication Date
ES2155166T3 true ES2155166T3 (es) 2001-05-01

Family

ID=24304605

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96203641T Expired - Lifetime ES2155166T3 (es) 1995-12-21 1996-12-19 Lineas inmortalizadas de celulas derivadas de tejidos cutaneos humanos normales y medio de cultivo sin suero, adaptados a su cultivo.

Country Status (23)

Country Link
US (2) US6423540B2 (es)
EP (2) EP0780469B1 (es)
JP (1) JP4404965B2 (es)
KR (1) KR100455006B1 (es)
CN (1) CN1154717C (es)
AT (1) ATE199390T1 (es)
AU (1) AU730222B2 (es)
BR (1) BR9612256B1 (es)
CA (1) CA2234118C (es)
CZ (1) CZ297289B6 (es)
DE (1) DE69611894T2 (es)
DK (1) DK0780469T3 (es)
ES (1) ES2155166T3 (es)
GR (1) GR3035719T3 (es)
HU (1) HU226195B1 (es)
IL (1) IL124191A (es)
NO (1) NO326190B1 (es)
NZ (1) NZ325814A (es)
PL (1) PL195108B1 (es)
PT (1) PT780469E (es)
RU (1) RU2215030C2 (es)
SK (1) SK283314B6 (es)
WO (1) WO1997023602A1 (es)

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CZ297289B6 (cs) * 1995-12-21 2006-10-11 Societe Des Produits Nestle S. A. Imortalizované bunecné linie a zivné médium
DK1071747T3 (da) * 1998-04-17 2004-11-29 Nestle Sa Immortaliseret cellelinie afledt af normale, humane hudvæv
US6964869B2 (en) 1998-07-13 2005-11-15 Wisconsin Alumni Research Foundation Method and composition for skin grafts
CN1087778C (zh) * 1999-03-02 2002-07-17 华东理工大学 杂交瘤细胞无血清培养基
DE19912798C1 (de) * 1999-03-10 2000-02-17 Andreas Jordan Verfahren zur Kultivierung von Krebszellen aus Humangewebe und Vorrichtung zur Aufbereitung von Gewebeproben
AUPR298901A0 (en) 2001-02-07 2001-03-08 McComb Foundation, Inc., The Cell suspension preparation technique and device
IL158526A0 (en) * 2001-04-24 2004-05-12 Wisconsin Alumni Res Found Method and composition for skin grafts
AU2002342613A1 (en) 2001-05-09 2002-11-25 Geron Corporation Treatment for wounds
US20030022369A1 (en) * 2001-05-18 2003-01-30 Helen Fillmore Differentiation of specialized dermal and epidermal cells into neuronal cells
EP1417629B1 (en) * 2001-07-06 2008-02-20 Lipomics Technologies, Inc. Generating, viewing, interpreting, and utilizing a quantitative database of metabolites
GB0208041D0 (en) 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US20050025737A1 (en) * 2003-07-30 2005-02-03 Sebagh Jean Louis Compositions containing melon extracts
CA2537462A1 (en) * 2003-09-18 2005-03-31 Raven Biotechnologies, Inc. Cell culture media comprising fructose as the primary energy source
WO2005071065A1 (en) * 2004-01-26 2005-08-04 Cognis France S.A.S Method for studying functional interactions between sensory neurons and keratinocytes or melanocytes
WO2006101834A1 (en) 2005-03-17 2006-09-28 Stratatech Corporation Skin substitutes with improved purity
DE602007008627D1 (de) * 2006-02-14 2010-10-07 Genetix Ltd Zellkulturmedium
WO2010065907A2 (en) * 2008-12-05 2010-06-10 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
KR101768632B1 (ko) * 2011-05-18 2017-08-17 (주)아모레퍼시픽 칼슘 펄스를 통한 배양상피세포층의 분리방법
BR112014000522B1 (pt) * 2011-07-12 2021-03-23 Foodchek Systems, Inc. Meio de cultura e método para cultivar salmonella e e. coli
WO2013129885A1 (ko) * 2012-02-28 2013-09-06 건국대학교 산학협력단 세포 배양액
WO2014142038A1 (ja) 2013-03-11 2014-09-18 Ｊｃｒファーマ株式会社 ヒト角膜上皮シートの製造法
AU2013205148B2 (en) 2013-03-14 2014-10-30 AVITA Medical Americas, LLC Systems and methods for tissue processing and preparation of cell suspension therefrom
KR20160065038A (ko) 2014-11-28 2016-06-08 (주)아모레퍼시픽 멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
CA2971150A1 (en) * 2015-01-26 2016-08-04 Halliburton Energy Services, Inc. Rotating superhard cutting element
CN105238739A (zh) * 2015-11-11 2016-01-13 朱宁文 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) * 2016-12-24 2017-03-22 严志海 一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) * 2017-05-12 2017-09-01 青岛赛欧凯普图乐生物医药有限公司 一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) * 2020-07-31 2022-02-18 上海尚瑞生物医药科技有限公司 一种无血清的黑素母细胞培养液及其培养方法
KR102583179B1 (ko) * 2020-11-27 2023-09-26 (주)엑셀세라퓨틱스 칼슘, 상피세포성장인자 및 알부민을 포함하는 각질형성세포 수립 또는 배양용 배지 조성물

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JPH10500669A (ja) * 1994-04-12 1998-01-20 アルザ・コーポレーション サイトカイン阻害薬に関するスクリーニング法
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CZ297289B6 (cs) * 1995-12-21 2006-10-11 Societe Des Produits Nestle S. A. Imortalizované bunecné linie a zivné médium

Also Published As

Publication number Publication date
GR3035719T3 (en) 2001-07-31
HU226195B1 (hu) 2008-06-30
CN1154717C (zh) 2004-06-23
PL327320A1 (en) 1998-12-07
CN1205737A (zh) 1999-01-20
US6423540B2 (en) 2002-07-23
BR9612256A (pt) 1999-07-13
US20020042129A1 (en) 2002-04-11
SK283314B6 (sk) 2003-05-02
KR19990071817A (ko) 1999-09-27
US6949381B2 (en) 2005-09-27
JP4404965B2 (ja) 2010-01-27
PL195108B1 (pl) 2007-08-31
RU2215030C2 (ru) 2003-10-27
WO1997023602A1 (en) 1997-07-03
KR100455006B1 (ko) 2005-01-13
NO982810L (no) 1998-08-21
US20020012993A1 (en) 2002-01-31
DE69611894T2 (de) 2001-09-13
EP0877797A1 (en) 1998-11-18
SK83598A3 (en) 1999-01-11
PT780469E (pt) 2001-06-29
NO326190B1 (no) 2008-10-13
HUP9903742A2 (hu) 2000-03-28
DK0780469T3 (da) 2001-03-26
IL124191A (en) 2004-06-01
EP0780469B1 (fr) 2001-02-28
CZ297289B6 (cs) 2006-10-11
CA2234118A1 (en) 1997-07-03
EP0780469A1 (fr) 1997-06-25
DE69611894D1 (de) 2001-04-05
AU1305497A (en) 1997-07-17
HUP9903742A3 (en) 2000-10-30
CA2234118C (en) 2009-04-07
NO982810D0 (no) 1998-06-18
CZ194598A3 (cs) 1998-11-11
AU730222B2 (en) 2001-03-01
JP2000506374A (ja) 2000-05-30
NZ325814A (en) 2000-10-27
ATE199390T1 (de) 2001-03-15
BR9612256B1 (pt) 2010-08-10

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